The effect of different combinations of local anaesthesia,sedative and non-steroidal anti-inflammatory drugs on daily growth rates of dairy calves after disbudding

AIM: To assess the effect of sedation and local anaesthesia (LA) at disbudding, and the addition of meloxicam or ketoprofen treatment, on weight gain in dairy calves following disbudding.

METHODS: Friesian-Jersey cross calves, from four dairy farms, were enrolled when 3–6 weeks old. All calves (n=271) were disbudded by veterinary personnel and randomly assigned to six groups: 136 were disbudded without sedation or LA, of which 31 received 20 mg meloxicam S/C and 75 received 150 mg ketoprofen I/M. A further 135 were disbudded with sedation (0.25 mg/kg xylazine I/M) and LA, of which 30 also received meloxicam and 75 received ketoprofen. Calves were weighed 3 days before, and 15 and 30 days after, disbudding (Day 0). Daily weight gain was analysed using mixed models and ANOVA.

RESULTS: Complete results were obtained from 263 calves. From Day ?3 to Day 15, the growth rate of calves disbudded without pain relief (0.53 (95% CI=0.47–0.60) kg/day) was less that of calves disbudded with some form of pain relief (0.65 (95% CI=0.62–0.68) kg/d; p=0.004). There was no difference between the effect of meloxicam or ketoprofen (p=1.00). An interaction between use of sedation and LA and additional non-steroidal anti-inflammatory drugs (NSAID) meant that NSAID treatment did not increase growth rates in calves disbudded with sedation and LA but did increase growth rates for calves disbudded without pain relief (p<0.05). From Day 16 to Day 30 there was no effect of NSAID treatment on growth rate, but calves receiving LA and sedation grew faster (0.74 (95% CI=0.69–0.80) kg/day) than calves disbudded without LA and sedation (0.66 (95% CI=0.61–0.71) kg/day; p=0.018). From Day ?3 to Day 30, calves disbudded with sedation and LA grew faster (0.71 (95%CI=0.64–0.77) kg/day) than calves disbudded without sedation and LA (0.60 (95% CI=0.55–0.65) kg/day; p=0.011). However, addition of NSAID to sedation and LA made no further difference to growth rates (p=0.69).

CONCLUSIONS: Dairy calves disbudded with no pain relief had slower growth rates than calves receiving pain relief. From Day 15 to 30 calves given no pain relief, or NSAID alone, grew more slowly than those receiving sedation and LA at disbudding. The addition of NSAID treatment to sedation and LA did not further increase growth rates.

CLINICAL RELEVANCE: This study adds to the evidence that pain management when disbudding is beneficial for calf productivity as well as calf welfare.     

An epizootic of bovine ephemeral fever in New South Wales in 2008 associated with long-distance dispersal of vectors

Objective To report the rapid transmission of bovine ephemeral fever ( BEF) virus from north-western New South Wales south to the Victorian border in January 2008 and to present data that suggests an uncommon meteorological event caused this rapid southward dispersal of vectors. Procedure The locations of reported clinical cases, data from sentinel herds and results from a survey of cattle in the southern affected area were examined to delineate the distribution of virus transmission. Synoptic weather charts for January 2008 were examined for meteorological conditions that may have favoured movement of vectors in a southerly direction. Results Cases of BEF and exposure to BEF virus in NSW were confirmed west of the Great Dividing Range, extending from the Queensland border to Finley, on the far North Coast and around the Hunter Valley. A low-pressure system moved south across the state on 18–19 January 2008, preceding the first cases of BEF in the south of NSW by 1–2 days. Conclusion Heavy rainfall in December 2007 provided a suitable environment for vector breeding, resulting in the initiation of and support for continuing BEF virus transmission in north-western NSW. The movement of a low-pressure system south across central western NSW in mid-January 2008 after the commencement of BEF virus transmission in the north-west of the state provided a vehicle for rapid southward movement of infected vectors.     

Vineyard yield estimation by combining remote sensing,computer vision and artificial neural network techniques

In viticulture, it is critical to predict productivity levels of the different vineyard zones to undertake appropriate cropping practices. To overcome this challenge, the final yield was predicted by combining vegetation indices (VIs) to sense the health status of the crop and by computer vision to obtain the vegetated fraction cover (F_c) as a measure of plant vigour. Multispectral imagery obtained from an unmanned aerial vehicle (UAV) is used to obtain VIs and F_c, which are used together with artificial neural networks (ANN) to model the relationship between VIs, F_c and yield. The proposed methodology was applied in a vineyard, where different irrigation and fertilisation doses were applied. The results showed that using computer vision techniques to differentiate between canopy and soil is necessary in precision viticulture to obtain accurate results. In addition, the combination of VIs (reflectance approach) and F_c (geometric approach) to predict vineyard yield results in higher accuracy (root mean square error (RMSE)?=?0.9 kg vine^?1 and relative error (RE)?=?21.8% for the image when close to harvest) compared to the simple use of VIs (RMSE?=?1.2 kg vine^?1 and RE?=?28.7%). The implementation of machine learning techniques resulted in much more accurate results than linear models (RMSE?=?0.5 kg vine^?1 and RE?=?12.1%). More precise yield predictions were obtained when images were taken close to the harvest date, although promising results were obtained at earlier stages. Given the perennial nature of grapevines and the multiple environmental and endogenous factors determining yield, seasonal calibration for yield prediction is required.

     

Platelet-Rich Plasma in the Treatment of Induced Tendinopathy in Horses: Histologic Evaluation

This study was carried out to evaluate the effect of platelet-rich plasma (PRP) on the treatment of tendinopathy induced in the superficial digital flexor tendon (TFDS) of horses, by using histologic evaluation. Six healthy crossbred geldings aged 8 to 15 years (12 ± 3) were used. The TFDS tendinopathy was provoked in both forelimbs, by intratendinous administration of 2.5 mg collagenase (2.5 mg/mL), and this procedure was considered as the beginning of the experimental phase. At 12 days after induction of the tendinopathy, the animals were subjected to the following treatments: (1) in the lesion caused in the right superficial digital flexor tendon (PRP-treated group), 2.5 mL PRP activated with calcium chloride at 0.0125 mol/L at concentrations from 320,000 to 500,000 platelets/μL, were injected; (2) in the tendinopathy of the left SDFT (control group), 2.5 mL 0.9% saline solution was administrated. Thirty-six days after the treatments, a biopsy of the injured area was performed for histologic evaluation. In both groups, the histologic analysis showed an increase in the fibroblastic density, as well as the presence of neovascularization, lymphocytes, and plasmocytes infiltrate and tissue organization at variable intensity. In the PRP-treated group, the SDFT was more organized, with the collagen fibers and fibroblasts being better arranged on the tendon matrix. The numbers of the fibroblasts and blood vessels did not differ between the groups. Histologic evaluation 36 days after tendinopathy showed that injuries under a single PRP treatment present a more uniform and organized tissue repair when compared with the control group.     

Grapevine cv. ‘Riesling’ water use in the northeastern United States

Vine water use was measured in a Vitis vinifera cv. Riesling vineyard located in New York. Vines were fully irrigated and were trained via vertical shoot positioning giving a narrow curtain intercepting about 30% of the incident light during the sunlight hours. Vine water use was estimated on six vines by sap flow gauges directly calibrated with whole canopy transpiration measurements. The regression analysis between estimates of transpiration showed that there were large differences between vines in the calibration values obtained. Sap flow monitoring started late in June, about 2 weeks after bloom, when the canopy already filled the trellis system, and continued until October. Results showed that vine water use during most of the summer days was between 1.0 and 2.0 mm day⁻¹, with peak values around 2.5 mm. The basal (e.g. vine transpiration/reference evapotranspiration) crop coefficient (K _cb) varied somewhat between days, but it was quite stable during the whole season. Averaged over the entire experimental period, the K _cb was 0.49. Some of the day-to-day variation in the K _cb was negatively related with daily average air vapour pressure deficit. This suggests that reference evapotranspiration models on grass may not be fully accurate for vines under these experimental conditions.     

Effect of Sperm Cryopreservation on the European Eel Sperm Viability and Spermatozoa Morphology

The main objective of the present work was to study the effect of cryopreservation of European eel sperm both on the sperm viability and the spermatozoa head morphology. Spermatozoa morphology was evaluated with computer-assisted morphology analysis after collection in fresh samples, after adding the freezing medium containing dimethyl sulfoxide as cryoprotectant and, finally, after the cryopreservation process and thawing. Cell viability was assessed, in both fresh and thawed samples, by Hoechst 33258 staining. Computer-assisted sperm analysis (CASA) was used to determine the percentage of motile cells and to measure motility parameters in sperm samples. A significant decrease of head perimeter (12.56%) and area (17.90%) was detected from spermatozoa in fresh to thawed samples, indicating that cells do not recover the original size after the cryopreservation process. CASA was used to measure the percentage of motile cells (51.9%) and spermatozoa motility parameters such as curvilinear, straight line and angular path velocities, as well as beating cross frequency. This technique was employed in the fresh sperm samples but proteins present at the freezing medium (L-alpha-phosphatidylcholine) made impossible to use this last technique in thawed samples. When sperm viability was assessed by Hoechst staining, a significant decrease of approximately 15% (73.10 vs 58.26%) of alive spermatozoa was registered from fresh to thawed samples. The percentage of motile cells measured by CASA in fresh samples (51.9%) was lower than the percentage of alive cells determined by Hoechst stainning, suggesting the existence of different batches of spermatozoa in different stages of development, even during the eight to tenth weeks of treatment, when the highest sperm quality was found.     

Response of Clementina de Nules citrus trees to summer deficit irrigation. Yield components and fruit composition

The effects of mid-summer regulated deficit irrigation (RDI) treatments were investigated on Clementina de Nules citrus trees over three seasons. Water restrictions applied from July, once the June physiological fruit drop had finished, until mid September were compared with a Control treatment irrigated during all the season to match full crop evapotranspiration (ET_c). Two degrees of water restrictions were imposed based on previous results also obtained in Clementina de Nules trees ( [Ginestar and Castel, 1996] and [González-Altozano and Castel, 1999]). During the RDI period, deficit irrigation was applied based on given reductions over the ET_c, but also taking into account threshold values of midday stem water potential (Ψ_s) of −1.3 to −1.5 MPa for RDI-1 and of −1.5 to −1.7 MPa for RDI-2. Results showed that water savings achieved in the RDI-2 treatment impaired yield by reducing fruit size. On the contrary, the RDI-1 strategy allowed for 20% water savings, with a reduction in tree growth but without any significant reduction in yield, fruit size nor in the economic return when irrigation was resumed to normal dose about three months before harvest. Water use efficiency (WUE) in the RDI trees was similar or even higher than in Control trees. RDI improved fruit quality increasing total soluble solids (TSS) and titratable acidity (TA). In conclusion, we suggest that the RDI-1 strategy here evaluated can be applied in commercial orchards not only in case of water scarcity, but also as a tool to control vegetative growth improving fruit composition and reducing costs associated with the crop management.     

Usefulness of trunk diameter variations as continuous water stress indicators of pomegranate (Punica granatum) trees

Pomegranate trees (Punica granatum L.) is a deciduous fruit tree included in the so-called group of minor fruit tree species, not widely grown but of some importance in the south east of Spain. Pomegranate trees are considered as a culture tolerant to soil water deficit. However, very little is known about pomegranate orchard water management. The objective of this research was to asses the feasibility of using trunk diameter variation (TDV) indexes, obtained by means of LVDT sensors, as a plant water stress indicators for pomegranate trees. The experiment was carried out with mature trees grown in the field under three irrigation regimes: control well watered trees; trees continuously deficit irrigated at 50% of the control regime (SDI); and trees that had a summer water stress cycle being irrigated at 25% of the control rates only in July and August (RDI). The seasonal variations of maximum diurnal trunk shrinkage (MDS) and trunk growth rates (TGR) were compared with midday stem water potential (Ψstem) measurements. During the course of the entire season, control trees maintained lower MDS values than the SDI ones. In the RDI treatment, as water restrictions began, there was a slow increase in MDS, in correspondence with a decrease in Ψstem. When water was returned at full dosage, the RDI quickly recovered to MDS and Ψstem values similar to the control. However, lower MDS for a given Ψstem values were observed as the season advanced. The magnitude of differences between well watered and deficit irrigated trees was much larger in the case of MDS than for Ψstem. However, the tree-to-tree variability of the MDS readings was more than four times higher than for Ψstem; average coefficient of variation of 7.5 and 36% for Ψstem and MDS, respectively. On the other hand, TGR did not clearly reflect differences in tree water status. Overall, results reported indicated that MDS is a good indicator of pomegranate tree water status and it can be further used for managing irrigation. However, the seasonal changes in the MDS-Ψstem relationship should be taken into account when attempting to use threshold MDS values for scheduling irrigation.