Molecular cloning and sequence analysis of interferon-gamma and interleukin-6 from Tibetan macaque (Macaca thibetana)

The cloning and sequence analysis of Tibetan macaque IFN-(gamma) and the IL-6 cDNAs are described. The Tibetan macaque IFN-gamma and IL-6 cDNAs were found to be 498 and 639 bp in length, with open reading frames encoding 165 and 212 amino acids, respectively. Homology analyses indicated that the identity levels of nucleotide and deduced amino acid sequences of IFN-gamma among primates ranged from 93.4 to 99.2%, and 87.3 to 99.4%, respectively, and that of IL-6 ranged from 92.6 to 99.8%, and 85.4 to 99.5%, respectively. Phylogenetic analysis based on amino acid sequences showed that the Tibetan macaque is most closely related to Old World monkeys, as compared to Hominoidea and New World monkeys. These findings provide insights into the evolution of primate IFN-gamma and IL-6 and additional valuable information regarding amino acid residues essential for their biological activity.     

不同氮肥浓度对薄壳山核桃苗木光合特性影响

以1 a生薄壳山核桃实生苗为研究对象,探讨浇施不同浓度尿素溶液对薄壳山核桃实生苗净光合速率、蒸腾速率、气孔导度、胞间CO2浓度、光合有效辐射等12个生理生态因子日变化的影响,以及各生理生态因子间的相关关系,为培育薄壳山核桃优质砧木提供合理施肥依据。采用完全随机试验设计,测定分别浇施0.3%、0.6%、0.9%浓度尿素溶液和对照处理薄壳山核桃实生苗的净光合速率等12个生理生态因子的日变化进程,并采用相关分析分析各因子间的相关关系,采用主成分分析法和通径分析法分析各生理生态因子对净光合速率的直接和间接影响。结果表明：薄壳山核桃实生苗净光合速率日变化曲线呈“双峰型”,有明显“午休”现象;各生理生态因子与净光合速率存在密切的相关关系,空气相对湿度是净光合速率变化的主要决策因子,气孔限制值也是重要的决策因子,蒸腾速率是净光合速率变化的主要限制因子;浇施不同浓度尿素溶液的薄壳山核桃实生苗具有比不施肥苗木更高光合效率,以0.6%浓度尿素溶液具有更高净光合速率;提高薄壳山核桃实生苗生长环境的空气相对湿度和限制苗木的蒸腾速率是提高薄壳山核桃实生苗净光合速率的重要措施。     

2008年山东省胶东地区鸡场疑似大肠杆菌的分离鉴定及药敏试验

试验对2008年春季山东省胶东地区疑似大肠杆菌病例进行病原分离鉴定及耐药性检测,共分离到大肠杆菌40株,鉴定出血清型35株,以O78、O5 、O24、O111四个血清型为主,约占定型菌株的80%.其中O78血清型占定型菌株的43% ,为优势血清型.细菌的耐药性检测结果显示,分离菌株呈现出不同程度的耐药性,对阿米卡星的高敏株为78%,头孢噻肟钠为70%,大观霉素、庆大霉素、硫酸安普霉素、硫酸粘菌素敏感菌株为60%～64%,乳酸环丙沙星、氟苯尼考,敏感菌株比例为50%～55%;硫酸新霉素、诺氟沙星、达氟沙星、盐酸沙拉沙星、恩诺沙星、盐酸多西环素的高敏菌株比例低于43%;林可霉素、金霉素、泰妙菌素、泰乐菌素、氨苄西林、阿莫西林对所分离的菌株几乎无抑制作用.结果表明,山东省胶东地区鸡致病性大肠杆菌血清型复杂,耐药性广泛.     

二氟沙星残留检测用单克隆抗体的制备及其鉴定

用DFX-BSA免疫BALB/c小鼠,采用显微克隆技术成功筛选出5株特异性分泌细胞株1a6、2b4、4a4、X1和X2。将X1株细胞株直接注入小鼠腹腔获取腹水,腹水纯化后效价达1:1638400。蛋白质含量为6.86mg/ml,单抗X1属IgG2a亚型。X1株单克隆抗体的检测标准曲线方程为Y=0.4813-3.1067X(R=0.9510),最低检测限(LOD)为2ng/ml,线性范围为0.002-5μg/ml,DFX对其IC50为0.085μg/ml。单抗对氟喹诺酮类药物有一定的交叉反应,对沙拉沙星最高可达10.9%,对兽医临床常用抗菌药物氨苄西林、卡那霉素、盐酸四环素、磺胺甲噁唑和氯霉素等没有交叉反应。Ci-ELISA反应中McAb对低浓度的乙腈和丙酮具有良好的耐受性,而低浓度的甲醇对McAb的结合力有一定的影响。     

徽县引进银杏品种光合作用特性研究

对从江苏泰兴引进甘肃的泰兴大佛指、七星果、大佛手、圆玲6号、大马玲等5个银杏优良品种的主要光合生理指标、光合日变化进行了比较研究,以净光合速率、光能利用效率、水分利用效率等参数对各品种进行了综合分析。试验结果表明:引进的5个品种(系)中,圆玲6号对强光和弱光利用能力均高,有较强的光合能力,非常适宜于徽县各种立地条件下栽植;大佛手对强光利用能力高而对弱光利用能力较差,抗旱能力最强,适宜栽植在山坡上光照条件较好的地方。在水分利用方面,除了大马玲外其余几个品种均高于当地品种。     

An overview of the “Three-North” Shelterbelt project in China

The "Three-North" Shelterbelt is the largest and most distinctive artificial ecological engineering project in China.It has been conducted since November 25,1978 in the "Three-North" (i.e.,Northeast China,North China and Northwest China) regions.In this study,the background and the essential benefits of the "Three-North" Shelterbelt project are summarized.The basic status and future development of the "Three-North" Shelterbelt project are also studied.As well,we tried to discuss the problems existing in the current shelterbelt system and the strategies to conduct this ecological project rationally.     

浙北春季大棚厚皮甜瓜品种比较试验

以嘉兴市主栽品种蜜天下、玉菇为对照,对8个厚皮甜瓜品种(组合)进行品比试验。试验结果表明,JN09-2产量最高,达2 418.4 kg/667 m2,品质与对照相当,且抗性较对照好,推广应用前景最好;明珠一号与明珠二号丰产性较好,早熟,但品质不及对照,适宜作春季早熟栽培;东方蜜一号、东方红为哈密瓜型厚皮品种,综合抗性较差,不易管理,但其商品外观优良,口感独特,建议栽培技术水平高的农户作为搭配品种适当种植。     

水稻近等基因系中5个抗旱主效QTL的验证

为了验证5个抗旱主效QTL的导入效果,将已精细定位的5个抗旱主效QTLs(DTY1.1,DTY2.1,DTY2.2,DTY3.1和DTY12.1)作供体建立近等基因系,利用与5个QTL位点紧密连锁的16个SSR分子标记对导入抗旱主效基因的23份家系进行目标QTL检测。结果显示,利用6个标记同时检测,获得携带DTY3.1 QTL的家系5株;利用2个标记同时检测,获得携带DTY12.1 QTL的家系5株;其他3个QTL位点未检测到目标QTL的家系;没有同时获得具备不同抗旱QTL的家系。相比较而言,携带DTY3.1 QTL以Apo为供体及携带DTY12.1 QTL以Way Rarem为供体导入效果更好。这些抗旱家系的获得,为抗旱品种的选育提供了新的抗旱基因来源。     

转录因子OsHOX4过量表达及T1代转基因植株中纯合系鉴定

OsHOX4基因是属于水稻植物同源结构域(HD-Zip)转录因子家族的一个成员。本研究利用农杆菌介导法将OsHOX4基因转入籼稻(Oryza sativa ssp.indica)IR64中,获得54株过量表达植株。我们利用southern杂交分析确定了各个株系的拷贝数后,利用TAIL-PCR方法对4个不同的单拷贝株系进行了研究,并在其中找出T-DNA在3个单拷贝株系染色体上的插入位点(分别为染色体5,8,10)。根据T-DNA在各个株系的插入位点分别设计特异性引物,从T1代转基因株系里鉴定出纯合的植株。我们观察到OsHOX4过量表达植株的分蘖数明显高于野生型,株高比野生型明显的变矮,在同一个株系的纯合和杂合的转基因植株表型也有很大的差异。在TAIL-PCR分析基础上,为从T1代转基因作物中鉴定出纯合的植株提供了方法和理论依据。     

Genetic dissection of intersubgenomic heterosis in Brassica napus carrying genomic components of B. rapa

Although strong intersubgenomic heterosis for seed production has been observed between “natural” domesticated Brassica napus (rapeseed, AACC) and a new type of rapeseed into which subgenomic components of Brassica rapa (AA) have been introgressed, the molecular genetic mechanism of this intersubgenomic heterosis is not understood. In this study, a recombinant inbred line population of new type rapeseed derived from a cross between B. napus and B. rapa, together with a population from a backcross with the parental line of B. napus, was used to identify single-locus quantitative trait locus (QTL) and interacting QTL pairs for yield and nine yield-related traits. More than half of single-locus QTLs and interacting QTL pairs detected were involved with the novel alleles induced by the introgression of B. rapa. The alleles directly from B. rapa A genome played a secondary role in contributing to intersubgenomic heterosis. Allelic and nonallelic interactions of both novel alleles generated by B. rapa introgression and the alleles directly from B. rapa A genome contributed to the intersubgenomic heterosis between “natural” domesticated rapeseed and new type rapeseed into which B. rapa had been introgressed. Six loci for fixed heterosis were identified and their possible applications are also discussed.