Differentiation of hog cholera and bovine virus diarrhoea viruses in pigs using monoclonal antibodies

Monoclonal antibodies against hog cholera and bovine viral diarrhoea viruses were assayed on organ tissue sections of experimentally infected animals. The animals had been infected simultaneously with both viruses. The antibodies were tested using an indirect immunofluorescence test and an indirect enzyme immunoassay with a biotin/streptavidin/peroxidase detection system. A polyclonal hyperimmune serum was used as a control in direct immunofluorescence tests. Both techniques based on monoclonal antibodies were more sensitive and more specific than the conventional test, the enzyme immunoassay being more sensitive than the immunofluorescence test. Small amounts of BVD viral antigen were demonstrable with monoclonal antibodies in most organ tissues.     

灌水量对不同类型晚稻生育及产量影响的初步研究

采取盆栽遮雨人工定量灌水的方法,探讨了常规晚稻和杂交晚稻前后两大生育时期不同灌水量对生育和产量的影响.结果表明:两类型晚稻品种前后两个生育时期节水灌溉都能达到高产之目的,但不同类型和不同生育时期的最佳灌水量和节水效果不同.     

对一起定点屠宰点宰杀病死猪案的查处及启示

1案由 2001年5月4日下午18时,我县金水兽医站检疫人员在槐树关食品站金水定点屠宰点执行检疫任务时,发现该场两头生猪患败血型猪丹毒而死亡,现场处理要求该点作销毁深埋处理,场地、圈舍进行无害化消毒.     

新疆高等寄生植物(二)——列当科

本文研究了新疆列当科2属23种植物,用检索表列出了它们的不同,总结了前人的工作,并对它们的分布、生境、寄主给予了描述。     

卷边板件屈曲分析的半能量法

A semi-energy method to analyse the buckling behaviour of edge-stiffened plates under combined compression and bending is presented in this paper, One unloaded edge of the plate is restrained elastically against rotation and the other is stiffened by a st     

桉苗灰霉病初步研究

按苗灰霉病(Botrytis cinerea Pers.ex Fr.)是近年在云南省新发现的病害,过去未曾报道。该病多发生在按苗幼嫩的茎上部和叶片上,可造成按苗迅速死亡。早春连绵阴雨,气温较低,致使该病易造成流行,该病的流行也与管理措施密切相关。采取平衡施肥,加强苗期管理是最有效的预防措施。发病期可施用75％百菌清可湿性粉剂1:500倍;或70％敌克松可湿性粉剂与等量的50％多菌灵可湿性粉剂混用,稀释1000倍常量喷雾,每7～10天一次,连续喷2～3次,可有效地控制按苗灰霉病蔓延流行。     

一类正规矩阵的反问题

In this paper, we consider the inverse problems for a kinds of normal matrices. Some necessary and sufficient conditions for existence of solution of these problems are given. The expression of the general solution is given.     

阻抗匹配平衡变压器低压电能的测量

According to the law for the current and voltage variance of the twophase system of the impedance matching balance transformer''s low equipment,we introduce the measuring method of real and reactive electric energy. A deep analysis for the error arising in     

两端不等弯矩作用下薄壁梁柱的弯扭屈曲

本文采用Ｒａｙｌｅｉｇｈ－Ｒｉｔｚ法求解两端不等弯矩作用下单轴对称开口薄壁截面梁柱弯扭屈曲临界荷载，分析中考虑了三种常见的边界条件以及屈曲前变形的影响，将本文弯扭屈由临界荷载理论值与试验值比较，吻合较好。     

牛腔前卵泡的简易机械分离方法

采用两种机械法分离牛腔前卵泡。方法一 (M -1) ,皮肤移植刀切割、剪碎、过滤镜下直接捡卵法。方法二 (M -2 ) ,皮质片剪碎、离心、悬浮、过滤镜下捡卵法。M -1平均每卵巢采卵数为 46 9个± 18 2个 ,M -2为 6 8 4个± 12 5个 ;处理时间M -1为 2 5 4min± 6 7min ,M -2为 46 3min± 16 8min ,两种方法采集腔前卵泡数量及处理时间均存在显著差异。平均每小时采集卵泡数分别为 99 5和 87 2个 ,M -1多于M -2。两种方法获得腔前卵泡大小分布规律也有明显差异 ,M -1采集腔前卵泡直径为 6 0～ 15 0 μm ,绝大部分是次级卵泡 ,而M -2获取腔前卵泡则偏小。总的看来 ,M -1处理时间短 ,回收效率高 ,方法简便 ,且分离卵泡直径较大 ,适于体外培养 ,是机械分离牛腔前卵泡的一种理想方法。