生态农业在元谋干热河谷生态旅游中的开发前景

元谋干热河谷生态旅游资源丰富,但缺乏对资源的总体结构组装和综合功能的发挥,生态农业在该区的兴起和完善,解决了该区发展生态旅游的这个关键问题。从元谋干热河谷的区位、旅游市场、资源等优势出发,初步提出利用生态农业对该区各种旅游资源进行组装后的多种具有生态景观结构功能、观光旅游功能、生态环境保护功能、学习参与娱乐功能的生态农业模式,探讨生态农业在该区生态旅游发展中的重要性、可行性及开发前景。     

基于灰色神经网络模型的重庆能源需求预测

能源是支撑人类生存、经济发展和现代文明不可缺少的物质基础,随着社会经济的发展,对于能源的需求也在不断增长,因此准确地预测能源消费需求对于制定能源规划具有重要的指导意义.基于灰色神经网络预测模型,以重庆市历年能源统计数据为样本,根据能源消耗总量与能源消耗增长率、万元GDP能耗、万元财政收入能耗、居民收入能耗、能源消费弹性等因素之间的相关性,对重庆"十三五"期间能源的消费需求进行了预测,结果表明:运用灰色神经网络模型能够较为科学地预测重庆未来能源的实际需求趋势,可为政府相关部门的决策提供参考.     

Association between ERK5 -322G/T polymorphism and genetic susceptibility to sporadic colorectal cancer

AIM: To investigate the correlation between extracellular signal-regulated kinase 5 ( ERK5 ) -322G/T polymorphism (rs3866958) and the susceptibility to sporadic colorectal cancer (CRC) in southern Chinese population. METHODS: ERK5 -322G/T genotypes were determined by Taqman-MGB probes in 835 CRC cases and 908 healthy controls. RESULTS: No significance of ERK5 -322G/T genotype distribution between CRC patients and controls was observed, but -322G/T decreased the susceptibility to CRC in fat people whose BMI was ≥ 24 kg/m². Compared to GG genotypes, the carriers with GT and TT genotypes had a significant decrease in the risk of CRC(OR=0.576,95%CI 0.413-0.804, P<0.01). CONCLUSION: ERK5 -322G/T polymorphism (rs3866958) has no significant relevance with sporadic CRC susceptibility, but decrease, the risk of CRC in people with fatness. The T variant genotype is an independent protective factor against sporadic CRC of overweight patients in southern Chinese population.     

Role of translationally controlled tumor protein in PRL-3-promoted metastasis of colon cancer cells

AIM: To study the role of translationally controlled tumor protein (TCTP) in the proliferation, migration and invasion of phosphatase of regenerating liver-3(PRL-3)-promoted colon cancer cells.METHODS: The vectors pAcGFP-C3 and pAcGFP-C3-PRL-3 were constructed and transfected into the colon cancer cell line LoVo.LoVo-PRL-3 cells stably expressing PRL-3 and LoVo-control cells were established. The expression levels of PRL-3 and TCTP in both cells were detected by Western blotting and real-time PCR. The specific siRNA sequence for TCTP mRNA and control-siRNA were synthesized and transfected into the LoVo-PRL-3 cells. TCTP expression at mRNA and protein levels in LoVo-PRL-3 was detected by Western blotting and real-time PCR 24 h, 48 h and 72 h after transfection. The proliferation, migration and invasion abilities of LoVo-control cells, LoVo-PRL-3 cells, TCTP-siRNA and control-siRNA cells were detected by CCK-8 assay and the method of Transwell cell culture chambers.RESULTS: The expression of TCTP at mRNA and protein levels in LoVo cells was significantly increased after PRL-3 transfection (P<0.05). TCTP mRNA was significantly inhibited 24 h, 48 h and 72 h after transfection of TCTP-siRNA (P<0.01). TCTP protein was also significantly inhibited 48 h and 72 h after transfection (P<0.01). Compared with LoVo-control cells, the proliferation, migration and invasion abilities of LoVo-PRL-3 cells were significantly enhanced (P<0.05). However, lowering the up-regulated expression of TCTP in LoVo-PRL-3 cells inhibited the proliferation, migration and invasion abilities (P<0.05). CONCLUSION: PRL-3 promotes proliferation, migration and invasion of colon cancer cells by up-regulating the TCTP expression. siRNA targeting TCTP may be an effective method for prevention and treatment of colon cancer cell metastasis.     

滴灌下不同供磷方式对马铃薯生长的影响

通过盆栽试验,研究了在滴灌施肥条件下不同供磷方式对马铃薯生长、产量、品质的影响。结果表明,施磷处理马铃薯的生物学产量显著高于不施磷处理,而不同供磷方式对马铃薯生物学产量的差异不显著;滴施磷肥处理有利于提高马铃薯块茎中的磷质量浓度;马铃薯块茎产量以50%磷肥基施、50%磷肥分10次追施的处理最高,且其商品薯率也最高,达到88.96%,比不施磷肥的对照处理高出6.54%;不同供磷方式对马铃薯块茎中的淀粉含量、干物质含量等品质指标的影响表现为差异不显著。     

1％阿维·鱼藤新型乳油防治柑橘红蜘蛛田间药效试验

采用1％阿维·鱼藤新型乳油对柑橘红蜘蛛进行田间药效试验。结果表明,1％阿维·鱼藤新型乳油500、1000和1500倍液3个处理对活动螨态均具优异的杀灭效果,药后1d,500倍液处理组防效显著高于对照药剂200阿维·哒3000倍液处理,二者各为99．70%±0．30％和93．64%±2.29％;药后5d,1％阿维·鱼藤新型乳油500、1000和1500倍液3个处理对活动螨态的防效与对照药剂相当,4者防效均高达94．72％以上。此外,1％阿维·鱼藤新型乳油1500、1000和500倍3个处理均对柑橘红蜘蛛螨卵具一定的杀卵效果,其中500倍处理组的杀卵作用较好,校正杀卵率达80．59%±2．51％。     

向日葵菌核病菌的生长发育和侵染循环

向日葵菌核病菌的萌发和子囊盘形成的最适温度是10~25℃,最适土壤相对湿度80%~90%,良好的通气条件。菌核的萌发不需要光,但子囊盘的形成需要一定的散射光。菌核在最适宜的条件下的萌发,子囊盘柄的长度最长不超过8 cm。因此,秋季深翻灭茬,可有效防止第二年菌核病的发生。     

广西斜纹夜蛾核型多角体病毒的分离鉴定

从广西部分地区采集到自然罹病死亡的疑似具有病毒症状的斜纹夜蛾幼虫，对其进行病毒的分离鉴定。用研磨病虫尸体得到的浆液，经分离纯化后感染连续人工饲养至第三代的健康斜纹夜蛾三龄幼虫，部分样品能诱发病毒病症状。经差速离心的提纯物，在相差显微镜下观察到外形不规则的颗粒，进一步在电镜下观察到菱形、五边型、六边形、近似圆形等形状的多角体。用依据SpltNFV DNA的egt基因保守序列设计的引物，对多角体中提取的DNA进行PCR扩增，得到了与预期一致的380bp片段。经测序分析，该PCR片段与Splt NPV egt基因在核苷酸水平上的同源性为99．4％，演绎的氨基酸水平上的同源性为100％。进一步对采自广西16个地域的疑似具有病毒症状的200份斜纹夜蛾幼虫样本进行PCR扩增，其中6个地域的62份样本呈PCR阳性，这表明，广西斜纹夜蛾自然种群中存在着SpltNPV的流行。     

川西南常绿阔叶林土壤呼吸及其对氮沉降的响应

通过原位进行低氮(LN,50 kg N/hm2.a)、中氮(MN,100 kg N/hm2.a)和高氮(HN,150 kg N/hm2.a)处理,研究了川西南天然常绿阔叶林土壤呼吸及其对模拟N沉降的响应。结果表明:(1)该森林土壤呼吸速率最大值612.21±77.82 mg CO2/m2.h出现在7月份,最小值108.95±17.01 mg CO2/m2.h出现在2月,年均土壤呼吸速率为348.00±157.83 mg CO2/m2.h,年均土壤呼吸通量为8.31±3.77 t C/hm2.a。采用双因素关系模型(Rs=aebTWc),土壤温度和土壤湿度共同解释了该常绿阔叶林2005年10月~2006年7月土壤呼吸速率季节变化的68.6%~73.9%,其拟合结果优于以土壤湿度或温度为参数的单因素关系模型。影响土壤呼吸速率的主导因子是温度,其地表温度变化响应的敏感程度Q10值为2.12,以土壤5 cm深处的温度为参数时,Q10值为2.51。(2)N沉降处理3个月后,该森林中HN和MN处理的土壤呼吸速率(309.43±17.24 mg CO2/m2.h,303.82±11.50 mgCO2/m2.h)均显著高于CK(269.28±13.78 mg CO2/m2.h)(P<0.05);处理4个月后,HN的土壤呼吸速率(272.42±13.25 mg CO2/m2.h)均显著高于MN(239.65±10.33 mg CO2/m2.h)、LN(229.10±9.90 mg CO2/m2.h)和CK(234.51±12.77 mg CO2/m2.h)(P<0.05);但处理7~10个月时,各处理之间无显著差异。研究表明,N沉降初期明显促进了常绿阔叶林土壤呼吸,后期无明显影响。     

嘉陵江中下游地区土地利用格局变化的动态监测与预测

在遥感和GIS技术的支持下,定量分析研究区1986～2000年的土地利用动态变化过程,以及土地利用各类型之间的相互转化状况,并对研究区土地利用动态变化的驱动力进行了分析。运用马尔柯夫原理,对研究区未来土地利用演变趋势进行模拟与预测。作者认为,必须在提高全民环保意识、坚决贯彻国家相应法规政策的前提下,科学规划城镇建设,严格控制城镇用地,大力发展生态农业．加强天然林保护,搞好退耕还林还草并有规划的植树造林,建立区域外补偿机制,以实现研究区国土资源的可持续利用。