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81.
AIM:To determine the migration mode of host lymphocytes into the intestinal graft by analyzing different lymphoid compartments of the graft. METHODS:2, 4, 6 days after transplantation, murine intestinal grafts were harvested for histologic assessment. Lymphocytes were collected from mesenteric lymph nodes (MLN), Peyer’s patch (PP), lamina propria lymphocytes (LPL) and intraepithelial lymphocytes (IEL) in the graft for analyzing by cytometry. RESULTS:At the day 2 and day 4, no evidence of rejection in intestinal allograft was found, while the rejection signs were apparent at the 6th day. At day 2 and day 4, host lymphocytes had rapidly replaced donor cell in graft MLN and PP. The migration of host lymphocytes into the LPL and IEL was not as fast as MLN and PP, however, the near-total replacement took place at the 6th post transplant day. The host lymphocytes were activated at the early stage of recruitment into the graft. CONCLUSION:The rapid recruitment of recipient lymphocytes and early activation could be responsible for the formidable rejection of intestinal transplantation. 相似文献
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AIM:To study the expression of telomerase inhibitor Pinx1 in acute leukemia cells and during the differentiation of acute promyelocytic leukemia cells,and to realize its effect on telomerase activity.METHODS:Realtime quantitative PCR with fluorescence probe hybridization was used to measure the expression of Pinx1 and hTERT mRNA in acute leukemia cells and during differentiation of NB4 cells induced by ATRA.The correlations between Pinx1 and hTERT expression were also analyzed.RESULTS:Pinx1 mRNA expression in acute leukemia samples (0.00312,5.42×10-4-0.024) was significantly higher than that in normal bone marrow mononuclear cells (7.89×10-4,0-0.00863,P<0.01).The expression of Pinx1 mRNA had significant positive correlation with hTERT mRNA expression (r=0.296,P<0.05).Pinx1 mRNA expression decreased during differentiation,its expression was positive correlated with hTERT mRNA expression (r=0.900,P<0.05).CONCLUSIONS:As an inhibitor of telomerase,however,Pinx1 also had the same direction of regulation with telomerase activity in acute leukemia cells,suggesting its expression variation may be a subsequent reaction induced by that of hTERT to stabilize telomerase activity.The exact mechanisms remained to be verified. 相似文献
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秦岭南麓贫困山区商南县自1999年实施退耕还林(草)工程以来,森林覆盖率达到52.7%,增加了1.5个百分点,林草覆盖率增加了4.6个百分点,但经济增长速度始终比较缓慢。为进一步了解退耕还林(草)工程对农村经济及退耕户带来的受益情况,以实地抽样调研为依据,通过对该县生态建设基本情况的深入调查,在初步掌握调查户普遍反映的主要问题基础上,依据该地区实际情况,提出了商南县生态与经济“双赢”互动模式,并对生态与经济互动途径提出了相关建议。 相似文献
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不同钠盐胁迫对高冰草种子萌发的影响 总被引:12,自引:0,他引:12
通过对高冰草种子在不同钠盐(NaCl、NaHCO3、Na2CO3)溶液中萌发情况的研究,旨在探明高冰草种子在不同盐胁迫下的萌发特性。结果表明:碱性盐(NaHCO3、Na2CO3)胁迫较中性盐(NaCl)胁迫显著降低了高冰草的发芽率。高冰草种子在NaCl浓度150mmol/L、NaHCO3浓度100mmol/L和Na2CO3浓度50mmol/L时萌发受到明显抑制。不同钠盐胁迫下高冰草种子胚根和胚芽的受抑制大小顺序为,中性盐NaCl胁迫:胚根<胚芽;碱性盐NaHCO3和Na2CO3胁迫:胚根>胚芽。 相似文献