首页 | 本学科首页   官方微博 | 高级检索  
文章检索
  按 检索   检索词:      
出版年份:   被引次数:   他引次数: 提示:输入*表示无穷大
  收费全文   17915篇
  免费   822篇
  国内免费   1843篇
林业   2387篇
农学   2668篇
基础科学   1215篇
  3128篇
综合类   4964篇
农作物   1198篇
水产渔业   786篇
畜牧兽医   2288篇
园艺   601篇
植物保护   1345篇
  2024年   44篇
  2023年   192篇
  2022年   416篇
  2021年   624篇
  2020年   590篇
  2019年   623篇
  2018年   409篇
  2017年   482篇
  2016年   545篇
  2015年   715篇
  2014年   677篇
  2013年   884篇
  2012年   1039篇
  2011年   1170篇
  2010年   1061篇
  2009年   1009篇
  2008年   1025篇
  2007年   999篇
  2006年   1018篇
  2005年   916篇
  2004年   508篇
  2003年   387篇
  2002年   298篇
  2001年   337篇
  2000年   410篇
  1999年   571篇
  1998年   530篇
  1997年   459篇
  1996年   371篇
  1995年   376篇
  1994年   309篇
  1993年   331篇
  1992年   264篇
  1991年   243篇
  1990年   182篇
  1989年   148篇
  1988年   134篇
  1987年   88篇
  1986年   48篇
  1985年   24篇
  1984年   24篇
  1983年   17篇
  1982年   14篇
  1981年   17篇
  1980年   11篇
  1979年   5篇
  1974年   6篇
  1968年   6篇
  1964年   3篇
  1963年   3篇
排序方式: 共有10000条查询结果,搜索用时 10 毫秒
31.
为了解掌握养殖场中环境微生物对养殖业和公共卫生造成的影响,初步对我国养殖场环境微生物进行了研究。对吉林、湖北等省份的部分养猪场和养鸡场环境采样,并进行大肠杆菌和沙门氏菌分离鉴定,同时对从鸡舍和猪舍内分离大肠杆菌和沙门氏菌进行动物致病性实验及对13种抗生素的耐药性实验。实验结果表明,所有实验的沙门氏菌和90.7%的大肠杆菌菌株对小白鼠有致病性;所有分离菌株对磺胺类药物的耐药性非常严重,其次是对四环素,对其他药物也有不同程度的耐药性。  相似文献   
32.
不同来源微粒子孢子间血清学关系的比较研究   总被引:1,自引:4,他引:1  
郭锡杰  黄可威 《蚕业科学》1994,20(3):154-157
应用玻片凝集法和酶标抗体法比较了几种不同来源微粒子孢子间的血清学关系,包括本所保存的家蚕微粒子孢子(BmN.b),从安徽绩溪和江苏东台的饲养蚕中收集到的微粒子孢子和从四川收集到的蓝叶虫(phyllobrotiacarmataBaly)微粒子孢子。结果表明,绩溪、东台微粒子孢子与BmN.b之间具有相同的血清学关系,而蓝叶虫微粒子孢子与BmN.b之间无血清学关系。  相似文献   
33.
It has been found that fibroblast growth factor receptor (FGF-FGFR) signaling can regulate the expression of adipocyte differentiation genes. FGF9 is one of the members of FGFs that mainly binds receptors FGFR2 and FGFR3. FGF9 is highly expressed in the adipose tissue of humans and mice, but there are few reports on the role of FGF9 in goat intramuscular adipocyte differentiation. Therefore, this study explored the effect of FGF9 on adipocyte differentiation through cell culture, interference, and overexpression. The expression of receptors FGFR1–FGFR4 in adipocyte differentiation and their effects on differentiation were detected to screen receptor gene of FGF9. Finally, the interaction between FGF9 and the receptor was tested by cotransfection. Our results showed that FGF9 interacts with FGFR2 to inhibit goat intramuscular adipocyte differentiation by regulating peroxisome proliferator-activated receptor gamma (PPARγ) and preadipocyte factor 1 (Pref1), which is a data support for subsequent pathway research.  相似文献   
34.
Southeast Asian macaques are hosts of a number of Plasmodium infections, some of which are transmittable to humans. During examination of blood films of five wild-caught long-tailed macaques Macaca fascicularis from South China, malaria infection was detected in one of the monkeys. In order to isolate this parasite for identification and characterization, we experimentally passed this parasite through both Assamese (M. assamensis) and rhesus (M. mulatta) monkeys by intravenous injection of infected blood. This parasite morphologically resembled Plasmodium inui, and had a typical 72 h quartan periodicity. This parasite was infective to Anopheles dirus mosquitoes, and salivary gland sporozoites appeared 13 days post feeding. Feeding by 20 infected An. dirus mosquitoes on another Assamese monkey produced infection with a prepatent period of 8 days. Molecular analysis of the small subunit rRNA genes and the mitochondrial genome confirmed this parasite as an isolate of P. inui. In spleen-intact macaques, the infection had a protracted duration with parasites being detected during the rearing of the infected monkeys for over two years. In summary, this study identified a P. inui isolate and successfully passed this parasite through Assamese monkeys by both intravenous inoculation and mosquito transmission.  相似文献   
35.
The fertilization capacity of sex-sorted sperms is seriously decreased, which inhibits its wide application. However, little information is still available about the effect of vitamin C (VC) and lycopene (Lyc) on the fertilization capacity of sex-sorted bull sperm. In this study, the washing medium and fertilization medium of sex-sorted sperm from three bull individuals were supplemented with different concentrations of VC (0, 1 × 10–3, 1 × 10–4, 1 × 10–5, 1 × 10–6 M) or Lyc (0, 1 × 10–4, 1 × 10–5, 1 × 10–6, 1 × 10–7). After washing twice and incubation for 1.5 hr, the malondialdehyde (MDA) level, phosphatidylserine (PS) translocation, membrane potential (Δψm) and IVF (in vitro fertilization) ability of sex-sorted sperm were investigated. For the sex-sorted sperm of bulls A, B and C, 1 × 10–3 M VC or 1 × 10–4 M Lyc treatment significantly decreased their MDA levels and PS translocation and increased their Δψm levels and cleavage rates after IVF. When blastocysts were concerned, 1 × 10–4 M Lyc significantly improved the blastocyst rates and their IFN-tau expression of bulls A and C. In conclusion, supplementation of 1 × 10–3 M VC or 1 × 10–4 M Lyc in washing and fertilization medium contributed greatly to improving the fertilization capacity of sex-sorted bull sperm during IVF procedure.  相似文献   
36.
不同品系西方蜜蜂基因组DNA多态性图谱构建及杂交分析   总被引:1,自引:1,他引:1  
本研究应用随机引物K(5'-CGGCCCCTGT-3')对不同品系西蜂的基因组DNA扩增,获得了能区分不同品系西蜂的多态性DNA图谱,从多态性图谱中选取一个共同片段K1680bp,标记成探针,探针K1680bp与不同品系西蜂的基因组DNA匀有杂交,从而证明片段K1680bp是西蜂蜂种的共性,为西蜂的鉴定提供了直接依据。  相似文献   
37.
Nested RT-PCR was used to investigate bovine viral diarrhea virus in 511 specimens collected from Chinese pigs exhibiting clinical symptoms between 2007 and 2010. Of these, 137 samples were BVDV-positive and the BVDV prevalence rate was 23.1% (9/39) in 2007, 27.7% (44/159) in 2008, 33.6% (34/101) in 2009, and 23.6% (50/212) in 2010. Twenty of 137 BVDV-positive samples were used for further genetic analysis of the 5'-UTR. Phylogenetic analysis revealed that they were BVDV-1 and subtyped into BVDV-1a, BVDV-1b, BVDV-1m, BVDV-1o and an unknown subgenotype. This study showed that BVDVs were highly prevalent in Chinese pig herds and appropriate measures should be taken to control BVDV prevalence in pig herds.  相似文献   
38.
香菇多糖是从香菇中提取分离的一种活性多糖,具有较强的免疫活性作用。本文综述了香菇多糖的免疫调节作用及其在家禽中的应用效果。  相似文献   
39.
当养犬爱好者想给自己挑选一头爱犬时,怎样选择小犬将会是一个至关重要的问题.笔者认为,了解小犬的饲养繁殖者很重要.因为小犬的饲养繁殖者直接关系到犬的质量.……  相似文献   
40.
屠宰企业的非洲猪瘟自检对切断病毒传播链条、打好非洲猪瘟攻坚战有着重要作用。为摸清四川省宜宾市屠宰企业的非洲猪瘟检测水平,对全市10个区县83家屠宰企业组织开展了非洲猪瘟检测能力比对工作。结果显示:屠宰企业检测人员偏少、岗位工作时间短、文化程度不高;不同区县、不同类别、不同组合的检测结果存在差异,有的差异较大。总的检测敏感性为49.72%,特异性为87.41%,准确率为66.56%。结果表明:屠宰企业的检测能力较为一般,监测风险预警效果不甚理想,有待加强。建议从保障人员队伍和加强操作培训两方面入手,提高屠宰企业非洲猪瘟检测能力。  相似文献   
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号