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971.
972.
杂交水稻(Oryza sative)汕优桂34F_1的三个花培品系89 AH-97,89 AH-167和88AHW-16叶绿素含量在分蘖盛期明显低于亲本汕优桂34F_1,在孕穗期则差异不大。但三个花培品系的净光合强度在分蘖盛期与汕优桂34F_1无明显差异,在孕穗期则显著地高于汕优桂34F_1。各品系的乙醇酸氧化酶活性均无明显差别。同时,三个花培品系的硝酸还原酶活性和核酸量均极显著高于汕优桂34F_1,其可溶性蛋白质含量在分蘖盛期和孕穗期亦均显著地高于汕优桂34F_1。经4℃低温处理后,三个花培品系脯氨酸含量增加率极显著地低于汕优桂34F_1,其超氧物歧化酶(SOD)活性下降率则极显著地高于汕优桂34F_1。而三个花培品系的酯酶和SOD等同工酶谱无质的差别,只有某些酶带的活性与汕优桂34F_1有不同程度的差异。以上结果表明,通过花药(粉)培养所得植株后代可相对地将杂交水稻亲本某些优良性状“稳定”下来。 相似文献
973.
黄晓霞 《甘肃农业大学学报》1997,32(4):381-385,388
在编辑《中国牦牛文献题录》过程中,检索了《中文科技资料目录》和《全国报刊索引》,以及进行了相应的联机检索。比较了两种中文核心期刊在检索时的查全率和查准率,从其变动中分析了影响查全、查准率的因素,以期从根本上改进中文检索工具的检索效率。 相似文献
974.
蒙山县西河镇龙蟠村15户果农,在2001年春种下迟熟椪柑15 hm2,在果农精心护理下,果树长势良好,2003年开始挂果. 相似文献
975.
976.
Lee YC Leu SJ Hung HC Wu HH Huang IJ Hsieh WS Chiu WT Hsieh MS Cheng TF Yang YY 《Veterinary immunology and immunopathology》2007,117(1-2):75-85
Severe acute respiratory syndrome (SARS) is a newly emergent human disease, which requires rapid diagnosis and effective therapy. Among antibody sources, immunoglobulin Y (IgY) is the major antibody found in chicken eggs and can be used as an alternative to mammalian antibodies normally used in research and immunotherapy. In this study, phage-expressing chicken monoclonal scFv antibody was chosen and characterized with phage display antibody technology. Truncated fragments of SARS-CoV spike protein were cloned in pET-21 vector and expressed in BL-21 Escherichia coli (E. coli) cells. After purification, the purity of these recombinant spike proteins was examined on SDS-PAGE and their identity verified with Western blot analysis using anti-his antibodies and sera from convalescent stage SARS-CoV-infected patients. Using these bacteria-derived proteins to immunize chickens, it was found that polyclonal IgY antibodies in the egg yolk and sera were highly reactive to the immunogens, as shown by Western blot and immunocytochemical staining analysis. A phage displaying scFv library was also established from spleen B cells of immunized chicken with 5 x 10(7) clones. After four panning cycles, the eluted phage titer showed a 10-fold increase. In sequence analysis with chicken germline gene, five phage clones reacted, with large dissimilarities of between 31 and 62%, in the complementarity-determining regions, one dominant phage 4S1 had strong binding to fragment Se-e, located between amino acid residues 456-650 of the spike protein and this particular phage had significantly strong binding to SARS-CoV-infected Vero E6 cells. Based on the results, we conclude that generating specific scFv-expressing phage binders with the phage display system can be successfully achieved and that this knowledge can be applied in clinical or academic research. 相似文献
977.
978.
Chunhong Zhao Jian'en Gao Yuefei Huang Guangqian Wang Mengjie Zhang 《Land Degradation \u0026amp; Development》2016,27(3):748-757
Vegetation plays an important role in soil erosion control, but few studies have been performed to quantify the effects of vegetation stems on hydraulics of overland flow. Laboratory flume experiments were conducted to investigate the potential effects of vegetation stems on Reynolds number, Froude number, flow velocity and hydraulic resistance of silt‐laden overland flow. Cylinders with diameter D of 2·0, 3·2 and 4·0 × 10−2 m were glued onto the flume bed to simulate the vegetation stems, and a bare slope was used as control. The flow discharge varied from 0·5 to 1·5 × 10−3 m3 s−1 and slope gradient was 9°. Results showed that Reynolds number on vegetated slope was significantly higher than that on bare slope because of the effect of vegetation stems on effective flow width. All the flows were supercritical flow, but Froude number decreased as D increased, implying a decrease in runoff ability to carry sediment. The mean flow velocity also decreased with D, while the velocity profile became steeper, and no significant differences were found in surface flow velocities among longitudinal sections on all slopes. Darcy–Weisbach friction coefficient increased with D, implying that the energy consumption of overland flow on hydraulic resistance increased. Reynolds number was not a unique predictor of hydraulic roughness on vegetated slopes. The total resistance on vegetated slopes was partitioned into grain resistance and vegetation resistance, and vegetation resistance accounted for almost 80% of the total resistance and was the dominant roughness element. Further studies are needed to extend and apply the insights obtained under controlled conditions to actual overland flow conditions. Copyright © 2015 John Wiley & Sons, Ltd. 相似文献
979.
Jun Huang Juan Wang Xining Zhao Hongbin Li Zilong Jing Xiaodong Gao Xiaoli Chen Pute Wu 《Land Degradation \u0026amp; Development》2016,27(4):946-954
Land degradation is recognized as a major environmental problem in rainfed fruit orchards on the Chinese Loess Plateau. Six treatments were used to investigate surface runoff and soil moisture by means of simulated rainfall experiments: (i) a control (clean cultivation) (CC); (ii) strip cock's foot (Dactylis glomerata L.) cover (SCF); (iii) strip crown vetch (Coronilla varia L.) cover (SCV); (iv) strip bird's foot trefoil (Lotus corniculatus L.) cover (SBF); (v) strip white clover (Trifolium repens L.) cover (SWC); and (vi) complete white clover cover (WCC). The time to runoff was significantly longer under WCC than under other treatments (p < 0·05). The total runoff volume and sediment yield were significantly greater under CC than under the vegetation cover treatments (p < 0·05). The mean infiltration rate under WCC and CC was the largest and lowest and differed significantly from that under other treatments (p < 0·05). The change of soil water storage was the largest under WCC and the least under CC. The soil moisture was significantly greater under SCF than under other treatments (p < 0·05). Treatment SCF seemed to be the best groundcover for rainfed sloping jujube orchards on the Chinese Loess Plateau. Copyright © 2014 John Wiley & Sons, Ltd. 相似文献
980.