Role of ghrelin in cell protection by up-regulating HSP70 through ERK1/2 signaling pathway in PC12 cells

AIM:To study the role of ghrelin in cell protection by up-regulating heat shock protein 70 (HSP70) and inhibiting apoptosis induced by oxidative stress through extracellular regulated protein kinases 1/2 (ERK1/2) signaling pathway in the PC12 cells. METHODS:Sodium nitoprusside (SNP) was used to induce oxidative stress injury in the PC12 cells. The cultured PC12 cells were divided into SNP-injured group (incubated with SNP at 0.5 mmol/L for 6, 12, 18 and 24 h), ghrelin pretreatment group (ghrelin at 100 nmol/L was given 30 min before adding SNP); HSP70 inhibitor group (quercetin at 10 μmol/L was added 60 min before ghrelin treatment), ERK inhibitor group (ERK 1/2 inhibitor PD98059 was added 60 min before ghrelin treatment) and control group (added same amount of culture medium only). The apoptotic rate was detected by flow cytometry. The protein expression was determined by Western blot and immunocytochemistry. RESULTS:Compared with control group, the apoptotic rate of PC12 cells in SNP-injured group was significantly increased (P<0.05). Compared with SNP-injured group, ghrelin (100 nmol/L) pretreatment significantly inhibited SNP-induced apoptosis of PC12 cells (P<0.05), and significantly up-regulated the protein expression of HSP70 (P<0.05). Time-effect analysis showed that ghrelin had the most significant effect at 18 h after SNP injury. Quercetin, an inhibitor of HSP 70, significantly reduced the anti-apoptotic effect of ghrelin (P<0.05). Ghrelin pretreatment promoted the phosphorylation of ERK1/2. ERK1/2 inhibitor PD98059 significantly inhibited the effects of ghrelin on up-regulation of HSP70 expression (P<0.05). CONCLUSION:Ghrelin upregulates the expression of HSP70 and inhibits the apoptosis in the PC12 cells induced by oxidative stress by promoting the phosphorylation of ERK1/2.     

温室机器人道路识别与路径导航研究——基于红外测距

针对温室内移动机器人的应用需求,提出了一种基于红外线测距的温室机器人自主导航算法,并使用模糊算法对导航误差进行控制,实现了温室机器人的精确自主移动功能。温室机器人导航过程中,当红外线接收管接受到红外线信号时,会产生一个光强电流,电流放大后可以输出一个模拟电压;根据电压值,通过编程计算,利用电压和距离的对应关系,可以得到机器人和标志物的距离误差;距离信息通过串口传输到PC机上,PC机利用模糊控制原理对距离误差进行判断,发出控制指令。实验测试发现:机器人导航的距离偏差平均值为-1.28cm,均方差为2.68,超调较小,可以实现较为精确的导航。     

Structure-Activity Relationship Study of the Neuritogenic Potential of the Glycan of Starfish Ganglioside LLG-3

LLG-3 is a ganglioside isolated from the starfish Linchia laevigata. To clarify the structure-activity relationship of the glycan of LLG-3 toward rat pheochromocytoma PC12 cells in the presence of nerve growth factor, a series of mono- to tetrasaccharide glycan derivatives were chemically synthesized and evaluated in vitro. The methyl group at C8 of the terminal sialic acid residue was crucial for neuritogenic activity, and the terminal trisaccharide moiety was the minimum active motif. Furthermore, the trisaccharide also stimulated neuritogenesis in human neuroblastoma SH-SY5Y cells via mitogen-activated protein kinase (MAPK) signaling. Phosphorylation of extracellular signal-regulated kinase (ERK) 1/2 was rapidly induced by adding 1 or 10 nM of the trisaccharide. The ratio of phosphorylated ERK to ERK reached a maximum 5 min after stimulation, and then decreased gradually. However, the trisaccharide did not induce significant Akt phosphorylation. These effects were abolished by pretreatment with the MAPK inhibitor U0126, which inhibits enzymes MEK1 and MEK2. In addition, U0126 inhibited the phosphorylation of ERK 1/2 in response to the trisaccharide dose-dependently. Therefore, we concluded that the trisaccharide promotes neurite extension in SH-SY5Y cells via MAPK/ERK signaling, not Akt signaling.     

喷油泵试验台智能量油系统的研究

为解决喷油泵试验台量油系统测量误差较大的问题，本研究通过采集喷油器针阀升程、喷油压力、转速等信号精确测得喷油量。     

PDA掌上电脑在广西森林资源一类调查中的应用

总结赛博星PDA掌上电脑在广西区第八次森林资源连续清查数据采集中的应用方法与优缺点.其数据采集功能模块包括样地调查总体信息、样地定位与测设、样地每木检尺记录、样地位置示意图及内业数据处理等.应用PDA进行数据采集具有提升数据采集精度和效率、提高调查质量和调查员责任心、方便质量检查和监督等优点,但也存在着CPU处理速度有限,屏幕避光性不好,电池供应能力弱,成本较高等问题.藉此,提出了相关建议.     

基于掌上电脑的农田信息快速采集与处理系统的研究

为了解决精细农业中空间信息及属性信息的快速获取与分析处理问题，提出并实现了基于掌上电脑的农田信息快速采集与实时处理系统。分析了具体实现系统功能的若干关键技术，如地理信息系统(GIS)、空间索引、Windows CE数据库技术、采样控制等因硬件环境的不同而产生的问题及相应的解决方案，又针对农场环境下的特殊性，提出并实现了一个面向对象的农场模型，以便于更好的描述农场环境及更方便的确定采样方案。作为一种实时系统，该系统可结合全球定位系统(GPS)和各种传感器，快速地测量和分析农田信息。应用该系统对两种GPS(Ag132与MAP330)进行了精度比较试验，快速而方便地证实了Ag132有更好的精确性与稳定性，更适于农业生产要求。试验的过程与结果也间接表明了该系统具有较好的实用性，为精细农业田间信息快速采集和处理设备的研制与开发奠定了基础。     

基于工控机和Visual C++的农机室内 模拟试验系统设计

该文对农机室内模拟试验系统进行了研究。采用面向对象的图形化编程语言Visual C++6.0编制农机试验台的运行工况监测系统,实现了试验平台运行工况参数的自动采集、数据存储、动态图形显示、分析和试验报表打印等。     

基于MSP430的温室大棚温度远程监控系统

研制了一种温室大棚的温度远程监控系统,系统以MSP430F149为控制终端的核心控制器,采用DS18B20作为温度传感器,利用GSM通信网络传输温度、故障等信息至农户手机或监控中心上位机。同时,详细阐述了系统温度采集、控制终端系统、GSM短信息系统和上位机监控系统等硬件设计思想。试验样机在某农户的蔬菜大棚中进行了试验,结果表明:系统能很好地完成温度控制、故障报警、GSM短信息传输功能,具有操作简单、智能化和人机界面友好等特点,在农业领域有良好的推广价值和应用前景。     

饲粮添加大豆磷脂对产蛋鸡生产性能和鸡蛋磷脂含量的影响

本试验旨在研究饲粮中添加大豆磷脂(SL)对产蛋鸡生产性能和鸡蛋磷脂含量的影响.选用360只22周龄健康产蛋鸡,随机分为5个处理,每个处理6个重复,每个重复12只鸡.5种试验饲粮中分别添加0、0.5%、1.0%、2.0%和4.0%的SL.试验期4周.试验结果表明:1)0.5%SL组平均蛋重最大(P>0.05),1.0%SL组平均日采食量最低(P>0.05),1.0%和2.0%SL组的料蛋比较低(P>0.05),均显著低于对照组(P<0.05),产蛋率以0.5%组最高(P>0.05),即饲粮中添加0.5%～2.0%的SL可改善产蛋鸡的生产性能;2)饲粮添加SL可以降低鸡蛋蛋黄水分含量(约1.7个百分点)(P>0.05)、1.0%、2.0%和4.0%SL组的蛋黄粗脂肪含量显著高于对照组(P<0.05);1.0%、2.0%和4.0%SL组全蛋中总磷脂显著高于对照组(P<0.05),1.0%和2.0%SL组蛋黄中总磷脂含量显著高于对照组(P<0.05),同时蛋黄胆固醇含量略有增加(P>0.05);1.0%、2.0%和4.0%SL组蛋黄和全蛋中磷脂酰胆碱(PC)含量显著高于对照组(P<0.05);2.0%SL组磷脂酰乙醇胺(PE)最高,但各组间差异不显著(P>0.05);PC/PE随饲粮SL添加量的增加而线性升高,0.5%、1.0%和2.0%SL组均显著高于对照组(P<0.05),4.0%SL组显著高于其他组(P<0.05).结果提示,饲粮添加一定水平的SL可改善产蛋鸡的生产性能,提高鸡蛋中总磷脂和PC含量.     

Retention of native vegetation within the plantation matrix improves its conservation value for a generalist woodpecker

The suitability of plantation monocultures for the conservation of forest animals is an issue under continous debate. The adaptability of forest dwellers and the forest management regime seem to play key roles. In this study, I investigated the habitat selection of a generalist bird, the great spotted woodpecker (Dendrocopos major), within a pine (Pine spp.) monoculture, as well as the importance of the native habitat features within the pine matrix for the species’ conservation. I compared 52 plots with woodpecker presence against 121 plots where the species was absent, as well as 68 nest-trees against 90 random ones. Regression analyses were used to investigate the habitat attributes involved in the habitat selection. Although the great spotted woodpecker is considered a generalist forest dweller, it shows a marked habitat selection. Based on presence/absence records, the woodpecker prefers well-forested patches with high levels of tree diversity and with good coverage of a secondary species such as the strawberry tree (Arbutus unedo). To excavate their nests, woodpeckers select large trees in patches where other trees are also larger, rejecting patches with a high number of small trees. The most striking conclusion from this work is the preference shown for native trees, especially Portuguese oaks (Quercus faginea), as nest-trees. This is noteworthy because native trees are smaller and they are surrounded by smaller trees than pines. These findings support that woodpecker conservation benefits from an increase of habitat heterogeneity, particularly by the retention of native woodland patches within the plantation matrix.