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AGPT and HA tests were employed for rapid diagnosis of PPRV infection in sheep and goats in Sudan. Forty lymph nodes and spleen samples from suspected cases of PPR in both sheep and goats were examined by AGPT and HA tests for detection of PPRV antigen. Viral antigen was detected from (77.5%) of the samples tested by AGPT and (92.5%) tested by HA test. The results of both tests revealed that HA test was more sensitive than AGPT for detection of PPRV antigen (Kappa statistics 0.4366). Another advantage of the HA test over AGPT was that it can differentiate PPRV from RPV. Thus the HA test represents a quick, easy, simple, cheap and reliable confirmatory test for the diagnosis of PPR and differential diagnosis of PPRV and RPV. The HA test was carried out using chicken, goat and pig RBCs. Chicken RBCs were found to be the most sensitive for detection of PPRV antigen, followed by goat then pig RBCs. The HA time when using chicken RBCs was 20–25 minutes, using goat RBCs was 25–30 minutes and using pig RBCs was 40–45 minutes. The distribution of PPR infection in four different regions of Sudan was investigated.  相似文献
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采用Klein—Defors悬浮杀灭方法,考察了那氏(NAS)制剂对H5N1和H9N2亚型禽流感病毒的杀灭效果。将不同浓度的NAS制剂药物组与利巴韦林药物对照组与H5N1亚型和H9N2亚型病毒各分别混合10min和30min。后10倍递进稀释成10个稀释度,接种鸡胚尿囊腔。培养一定时间后采集尿囊液进行血凝试验(HA),计算NAS制剂对两种亚型禽流感病毒在不同时间的杀灭率。结果表明,以1:40、1:80、1:160、1:320、1:640的稀释浓度与10^7-63 EID50禽流感H5N1亚型病毒液作用10min,病毒杀灭率分别为99.99%、99.99%、32.39%、0、0;作用30min杀灭病毒率分别为100%、99.99%、99.43%、0、0。与10^0.17 EID50 H9N2亚型禽流感病毒液作用10min,其杀灭病毒率分别为99.99%、99.99%、32.39%、0、0;作用30min病毒杀灭率分别为100%、99.99%、90%、0、0。提示NAS制剂对这两种病毒都有较好的杀灭作用,而且杀灭效果与时间有关。  相似文献
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为进一步优化猪细小病毒HA、HI微量法试验,比较了不同红细胞悬液浓度、不同液体体积、不同形状微量板对试验的影响.结果表明,豚鼠红细胞悬液浓度由原来的0.25%提高到0.5%,用50 μL体积进行HA试验,用25 μL体积进行HI试验,在“V”型微量板上的试验结果更清晰、更易判断.  相似文献
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