休闲农园节约建设规划设计的探索与实践

休闲农业是乡村经济发展的重要途径,也是乡村振兴的有力推手.休闲农园是实现农业多功能、三产融合、增产增收的重要载体.目前,资金短缺成为休闲农园发展的桎梏,研究其低成本打造及管理意义重大.从规划设计角度出发,结合多年规划设计实践经验,对休闲农园的低成本投入、低成本管理、集约高效生产、节约型景观美化提升以及绿色生态可持续发展等方面做了有益探索,以期为乡村振兴添砖加瓦.     

Protective efficacy of an H5/H7 trivalent inactivated vaccine produced from Re-11, Re-12, and H7-Re2 strains against challenge with different H5 and H7 viruses in chickens

We developed an H5/H7 trivalent inactivated vaccine by using Re-11, Re-12, and H7-Re2 vaccine seed viruses, which were generated by reverse genetics and derived their HA genes from A/duck/Guizhou/S4184/2017(H5 N6)(DK/GZ/S4184/17)(a clade 2.3.4.4 d virus), A/chicken/Liaoning/SD007/2017(H5 N1)(CK/LN/SD007/17)(a clade 2.3.2.1 d virus), and A/chicken/Guangxi/SD098/2017(H7 N9)(CK/GX/SD098/17), respectively. The protective efficacy of this novel vaccine and that of the recently used H5/H7 bivalent inactivated vaccine against different H5 and H7 N9 viruses was evaluated in chickens. We found that the H5/H7 bivalent vaccine provided solid protection against the H7 N9 virus CK/GX/SD098/17, but only 50–60% protection against different H5 viruses. In contrast, the novel H5/H7 trivalent vaccine provided complete protection against the H5 and H7 viruses tested. Our study underscores the importance of timely updating of vaccines for avian influenza control.     

Zerumbone attenuates MPP+-induced cytotoxicity in human neuroblastoma SH-SY5Y cells by inhibition of oxidative stress

AIM: To investigate the role of zerumbone (ZER) in 1-methyl-4-phenylpyridinium (MPP⁺)-induced cytotoxicity of human neuroblastoma SH-SY5Y cells. METHODS: Human neuroblastoma SH-SY5Y cells were cultured in vitro and the protective effect of ZER against MPP⁺-induced cytotoxicity was measured by CCK-8 assay. Flow cytometry was used to determine the apoptosis and reactive oxygen species (ROS). The expression of Parkinson disease protein 7 (PARK7) was knocked-down by using PARK7-specific short hairpin RNA (shRNA). The protein levels of PARK7, nuclear factor E2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) were determined by Western blot. RESULTS: MMP⁺ remarkably reduced the cell viability in a dose-dependent and time-dependent manner. The SH-SY5Y cell injury model was established by treatment with MPP⁺ at 600 μmol/L for 24 h. ZER up-regulated the protein levels of PARK7 and Nrf2 (P<0.05), alleviated apoptosis (P<0.05), and reduced ROS production (P<0.05) in the SH-SY5Y cell injury model. Meanwhile, N-acetyl-L-cysteine (NAC) had the similar functions. Moreover, significant reductions in the protein levels of Nrf2 and HO-1 (P<0.05), and obvious increases in apoptosis (P<0.05) and ROS level (P<0.05) were demonstrated in PARK7-knockdown cells. CONCLUSION: ZER protects SH-SY5Y cells against MPP⁺-induced cytotoxi-city, which may be related to activation of PARK7/Nrf2/HO-1 pathway, and subsequent attenuation of oxidative stress and apoptosis.     

右江流域水生态补偿机制研究初探

为保护右江流域生态环境,协调郁江乃至珠江上下游各方经济利益,更好的激励上游地区生态环境保护行为,通过分析右江流域水生态现状、存在问题、现有生态补偿政策环境及其在郁江流域、珠江流域中的地位和作用,利用外部性理论,分析提出了右江流域水生态补偿模式。通过对标对表有关生态保护规划和区划成果,立足百色市实际,提出了右江流域水生态补偿内容、补偿主客体、补偿标准和补偿方式,初步构建右江流域水生态补偿机制框架,并提出有针对性的补偿政策建议,为水生态补偿实施提供了参考。     

CRISPR/cas系统及其在家禽上的应用研究进展

CRISPR/cas是一种获得性免疫防御系统,广泛存在于细菌和古细菌中。Strep tococcus pyogenes cas9(Spcas9)作为目前研究最多、最清楚的效应蛋白,因其酶活性高和靶点广而被应用于生命科学各个领域,但Spcas9分子量大、脱靶率高等缺点在一定程度上限制了其应用。随着CRISPR系统技术被深入开发,一些新效应蛋白被发现,本文就CRISPR/cas系统分类、原理、新效应蛋白发现及cas9(S.Pyogenes)多种变构体技术开发在及家禽上的应用进行综述,为相关领域的研究提供参考。     

基于经济效益与生态效益的食用菌产业优化评价

为了优化食用菌产业结构,促进食用菌产业的可持续发展。给出了调整优化食用菌产业结构、促进食用菌产业生态经济建设的一些措施;提出重视经济效益和生态效益,合理引导发展中小食用菌企业的思路。对食用菌产业优化和生态经济的发展具有一定的参考价值。     

JT6120乘客座椅靠背曲线静态舒适性评价研究

The aim of this research was to evaluate a passenger seat of a new type bus in terms of eomfort. A fuzzy set model of multistage comfort scale (MCS) was adopted to the assessment of comfort, together with the techniques of human backshape and EMG measures     

交沙霉素等四种药物对雏鸡隐孢子虫病的预防效果

对经口感染(4.0×10~5个/鸡)贝氏隐孢子虫(Cryptosporidium baileyi)卵囊的雏鸡在其饲料中分别添加交沙霉素(4.0g/kg)、复方新诺明(4.9g/kg)、大蒜素(400mgkg/)、甲硝唑(2.0g/ks),任鸡自由采食,连用7d.结果表明,实验鸡潜隐期延长,粪中卵囊数显著降低,体重明显增加,血液学值及血清酶学指标与健康对照鸡无明显差异.由此证明,这4种药物对实验性雏鸡隐孢子虫病有不同程度的预防作用.     

L波段低噪声放大器的电路设计与性能分析

闸述了L波段低噪声晶体管放大器中各种降低噪声措施的基本原理,给出了低噪声电路设计的一般原则,以及L波段卫星地面接收系统前端低噪声高频放大器输入级中噪声匹配电路的设计举例.