调理素的研究进展

简述了补体、抗体、C-反应蛋白、血清淀粉样蛋白、甘露糖凝集素、表面活性蛋白、纤粘蛋白等七类调理素的研究,报道了新发现的调理素低密度脂蛋白和高密度脂蛋白,并对抗感染免疫学研究的新视角进行了展望。     

免深耕土壤调理剂在西瓜地上的试验初报

在土壤中施用浓度分别为2 000倍液、1 000倍液和500倍液的"Agri-Sc"免深耕土壤调理剂溶液.结果表明,与CK相比,"Agri-Sc"免深耕土壤调理剂对土壤容重、含水量、孔隙度及西瓜根、蔓的生长的影响均达到显著或极显著水平.对于黄棕壤的西瓜地,喷施"Agri-Sc"免深耕土壤调理剂最宜的浓度为1 000倍液.     

Characterization of the Dual Functions of LvCrustinVII from Litopenaeus vannamei as Antimicrobial Peptide and Opsonin

Crustin are a family of antimicrobial peptides that play an important role in protecting against pathogens infection in the innate immune system of crustaceans. Previously, we identified several novel types of crustins, including type VI and type VII crustins. However, their immune functions were still unclear. In the present study, the immune function of type VII crustin LvCrustinVII were investigated in Litopenaeus vannamei. LvCrustinVII was wildly expressed in all tested tissues, with relatively high expression levels in hepatopancreas, epidermis and lymphoid organ. Upon Vibrio parahaemolyticus infection, LvCrustinVII was significantly upregulated in hepatopancreas. Recombinant LvCrustinVII (rLvCrustinVII) showed strong inhibitory activities against Gram-negative bacteria Vibrio harveyi and V. parahaemolyticus, while weak activities against the Gram-positive bacteria Staphylococcus aureus. Binding assay showed that rLvCrustinVII could bind strongly to V. harveyi and V. parahaemolyticus, as well as the cell wall components Glu, LPS and PGN. In the presence of Ca²⁺, rLvCrustinVII could agglutinate V. parahaemolyticus and enhance hemocyte phagocytosis. The present data partially illustrate the immune function of LvCrustinVII, which enrich our understanding on the functional mechanisms of crustins and provide useful information for application of this kind of antimicrobial peptides.