黑木耳醇提物清除自由基能力及对α-淀粉酶抑制效果研究

目的:研究不同黑木耳醇提物清除自由基及抑制α-淀粉酶的活性功能.方法:以黑木耳为原料,在料液比1∶60、水浴温度40℃、浸提时间3h的条件下,利用不同体积分数乙醇作为提取溶剂得到不同黑木耳粗提物,将粗提物过滤并旋转蒸发至相同体积经干燥后冻存;进行体外自由基清除及对α-淀粉酶抑制作用研究.结果:乙醇体积分数为50％,DP...     

亚硒酸钠对豇豆品质和活性氧的影响

【目的】探究不同浓度的亚硒酸钠溶液浸种处理对豇豆果实品质和活性氧的影响.【方法】在(25±1)℃条件下,将‘美国无架豇豆’在0、2、4、6、8 mg/L的亚硒酸钠溶液中浸种9 h,每隔3 h换1次溶液.按常规方法种植,在果实挂果约40 d时取样,测定果实品质等生理指标.【结果】适量亚硒酸钠溶液处理可极显著地提高豇豆的可溶性蛋白质、维生素C、可溶性糖含量,随着亚硒酸钠溶液浓度的升高,这3种物质含量呈峰型变化,当溶液浓度为4 mg/L时达到峰值;不同浓度的亚硒酸钠溶液处理均极显著地提高了豇豆的抗坏血酸过氧化物酶(APX)、超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性,随着溶液浓度的提高,酶活性呈先升后降趋势,当溶液浓度为6 mg/L时达到最大值,但高浓度的亚硒酸钠处理(8 mg/L)对SOD活性的增加并无抑制作用;不同浓度的亚硒酸钠溶液处理均降低了豇豆中O₂^-·生成速率和H₂O₂、丙二醛(MDA)、硝酸盐含量,整体表现为先降后升,溶液浓度为4 mg/L时达到最小值.【结论】不...     

干旱胁迫对甜高粱幼苗光合色素、保护酶活性及活性氧代谢的影响

为探究甜高粱幼苗对干旱胁迫的响应机制和对干旱胁迫的忍受范围,通过盆栽试验的方式,设置土壤相对含水量65％～70％(CK)、55％～60％(T1)、40％～45％(T2)、30％～35％(T3)和20％～25％(T4)5个水平,研究干旱胁迫对甜高粱叶片光合色素含量、保护酶活性及活性氧代谢的影响.结果 表明,随着干旱胁迫强...     

锌对花生活性氧代谢、衰老和产量的影响

为探明锌肥对花生叶片光合机构活性氧代谢和衰老的调控机制,通过大田和室内实验相结合的方法,研究锌肥(ZnSO₄·7H₂O)施用量对花生生长过程中衰老相关参数和产量的影响。结果表明,随着施锌量的增加,花生的叶绿素含量、净光合速率、硝酸还原酶活性、过氧化氢酶活性和产量呈现出先升高后降低的趋势,光呼吸速率、米勒反应和丙二醛含量先降低后升高。适当施用锌肥不仅可以促进活性氧的清除,而且可以抑制活性氧的产生,锌肥通过活性氧的产生和清除对花生衰老进行调控。     

非1B/1R类型小麦雄性不育系KTSP3314A败育的生化机制

为了揭示K型非1B/1R类型小麦不育系雄性败育的生化机制。利用K型非1B/1R类型小麦雄性不育系KTSP3314A,其同型保持系TSP3314B,以K型1B/1R类型小麦雄性不育系K3314A、其同型保持系3314B为对照,分别对其叶片和穗子在不同发育时期的超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)的活性和丙二醛(MDA)含量的变化进行测定。结果表明,K型非1B/1R类型小麦不育系中SOD,POD及CAT活性变化与K型1B/1R类型小麦雄性不育系酶活性变化一致,K型非1B/1R类型雄性不育小麦叶片和穗子的超氧化物歧化酶(SOD)后期则较低,过氧化氢酶(CAT)、过氧化物酶(POD)的活性后期较高,丙二醛(MDA)含量后期较高。说明二者的雄性败育生化机制基本一致,但前者比后者败育的时期较晚。且超氧化物歧化酶(SOD)活性与雄性育性有关。     

植物衰老的分子机制及其调控

综述了植物衰老的意义和影响衰老的内部、外部因素 ,并从分子水平上探讨了植物衰老的机制及其遗传学调控     

Effect of Psammaplin A on in vitro Development of Bovine Aging Oocytes

To investigate the effect of histone deacetylation inhibitor Psammaplin A (PsA) on the development of bovine aging oocytes in vitro,oocytes were randomly divided into control group,aging group and 50 mmol/L PsA treated aging group (PsA group).Immunofluorescence staining and JC-1 were used to detect the blastocyst rate of bovine oocytes after parthenogenetic activation,the number of cells in blastocysts,apoptosis,reactive oxygen species (ROS),glutathione (GSH) and mitochondrial membrane potential intensity of embryos.The results showed that the blastocyst rate of the aging group was significantly lower than that of PsA and control groups (P<0.05).The blastocyst rate of PsA group was not significantly different from that of control group (P>0.05).The number of cells in the blastocysts of control group and PsA group were significantly higher than that of aging group (P<0.05).The number of cells in the blastocysts of PsA group was not significantly different from that of control group (P>0.05).The apoptosis rate in aging group was significantly higher than that of control and PsA groups (P<0.05),the apoptosis rate of PsA group was significantly higher than that of control group (P<0.05).The GSH level of MⅡ oocytes in aging group was significantly lower than that of control and PsA groups (P<0.05).There was no significant difference in GSH level between control and PsA groups (P>0.05).The ROS level of the embryos in aging group was significantly higher than that of control and PsA groups (P<0.05).The ROS level in PsA group was significantly higher than that of control group (P<0.05).The mitochondrial membrane potential of early embryos of aging group 4-8 cells was significantly lower than that of control and PsA groups (P<0.05).The mitochondrial membrane potential intensity of control group was significantly higher than that of PsA group (P<0.05).In summary,PsA could effectively delay the aging of bovine oocytes and improve the quality of oocytes.     

针灸抗衰老作用研究进展

针灸保健抗衰老历史悠久,早在2000多年前《黄帝内经》中对针灸抗衰老理论和有关针灸临床治疗就有详细的记载,为后世针灸抗衰老医学发展奠定了基础。晋代《针灸甲乙经》、明代杨继洲《针灸大成》等针灸著作对针灸抗衰老及因衰而致的老年病防治理论与方法都有不同程度的论述,丰富了针灸抗衰老的内容…。现代研究认为针灸主要有调整内分泌、调节免疫、调整中枢神经系统、清除自由基、补充微量元素、调整脂质代谢、调控衰老基因、促进物质代谢及强身健体等功能。现已明确衰老是内源性因素和外源性因素共同作用的结果,内源性衰老（自然衰老）为不可避免的渐进过程;外源性衰老（如风、光、热、烟、化学物质等）中以紫外线作用（光衰老）最为明显。针灸抗衰老的原则为未病先治,未老防衰、早期诊断、早期治疗、滋补脾胃肾、调整阴阳、以平为期。     

肌肽活性的生化基础及其应用

肌肽是由β-丙氨酸和L-组氨酸组成天然活性二肽,可螫合过渡金属离子,具有抗氧化及抗衰老等生物学活性,其游离氨基羧基及咪唑氮可有效接纳质子或羟基,稳定溶液pH等。就肌肽活性的生化基础及应用研究作一综述。