玉米秸秆消化率全基因组关联分析

48h体外干物质消化率（48h in vitro dry matter digestibility, 48h IVDMD）是衡量青贮玉米品质的重要指标。为了初步探究玉米秸秆消化率的分子遗传机理,以341份玉米自交系为材料,于2018年在沈阳和通辽种植,收获后测定秸秆48h IVDMD。利用全基因组重测序获得的6 276 612个高质量SNPs进行全基因组关联分析,共检测到153个与玉米秸秆消化率显著相关的SNPs位点（P<1.0×10^-6）,4个SNPs显著水平在P<1.0×10^-8以上;共找到38个秸秆消化率的候选基因,主要涉及细胞生长发育、防御反应和信号转导等生物学功能。     

马铃薯枯萎病和干腐病药剂防治筛选试验报告

马铃薯产业是宁夏“1+4”特色优势产业,是西吉县所有农作物中种植面积最大、涉及农户最多、比较效益最高的农民脱贫致富的主导产业。近年来西吉县马铃薯产业由商品薯种植大县向种薯繁育大县的转变,推动了农业增效,农民增收的快速发展。但各类土传性病害的发生影响了马铃薯产业的发展,同时磋商了农民种植的积极性。为了测定筛选出对马铃薯枯萎病和干腐病防治效果好、成本较低药剂,为大田防治提供科学依据。     

Changes in photosynthesis,growth, and sugar content of commercial sugarcane cultivars and Erianthus under flood conditions

Sugarcane (Saccharum spp.) is an economical crop in the tropical and subtropical countries. However, because of global climate change, flooding has become problematic, particularly during the rainy season, in Thailand. We investigated the effects of floods on three commercial sugarcane cultivars, namely NiF8, U-thong 6 (UT6), and U-thong 9 (UT9), as well as Erianthus spp. Growth was assessed using a pot experiment in a glasshouse with two treatments: (1) control and (2) 60 d of flooding followed by 30 d of normal conditions. In comparison with control, during prolonged flooding, Erianthus showed greatly decreased CO₂ assimilation, whereas NiF8, UT6, and UT9 showed slightly declined CO₂ assimilation. Growth in plants subjected to 60 d of flooding was less influenced by floods while sucrose content was not affected except in UT6. During flooding, some roots died, resulting in plants compensating adventitious roots to offset the negative effects of root death and to assist them in maintaining their growth, which appeared from the submerged nodes, with different characteristics for each cultivar. However, 30 d after draining, roots remained damaged, while adventitious roots died, resulting in lesser growth as compared with the control, but it did not significantly affect sucrose content and sugar yield. This study suggests that sugarcane plants need to produce the adventitious roots to compensate their roots’ death during flooding and require time to recover their root system after flooding for obtaining the optimum yield and quality at harvest.     

Compatibility of root growth and tuber production of potato cultivars with dynamic and static water‐saving irrigation managements

The important root characteristics of root length density (RLD) and root mass density (RMD) generally differ among irrigation managements and potato cultivars. The objective of this study was to investigate the RLD and RMD variations and their functional relationships with gross potato tuber yield for two commercial potato cultivars, Agria and Sante, under different irrigation strategies. Full irrigation and water‐saving irrigation strategies, deficit and partial root drying irrigations, were applied statically (S) and dynamically (D) based on daily crop evapotranspiration. Results showed that SPRD had significantly greater RLD (3.64 cm/cm³) and RMD (132.7 μg/cm³) than other irrigation treatments. Between the potato cultivars, Agria had significantly larger values of RLD (3.50 cm/cm³) and RMD (138.7 μg/cm³) than Sante. The functional relationship between the root growth characteristics and tuber yield showed that under water‐saving irrigations, Agria increased root mass at the expense of gross tuber yield but Sante increased root mass to maintain larger gross tuber yields. However, Agria produced more roots and gross tuber yield than Sante, and it is concluded that Agria is a more drought‐tolerant potato cultivar, which is recommended for tuber production in regions where water might be scarce. It was shown that larger root production in potatoes was associated with improved tolerance to water stress.     

Kernel vitreousness and protein content: Relationship,interaction and synergistic effects on durum wheat quality

Four sets of durum samples were used in this study to further understand the interrelationships among hard vitreous kernels (HVK), protein content, and pigment concentration, with a focus on the interaction and synergistic effects of protein content and vitreousness on durum quality. HVK level increases with higher protein content in the range of 9.5–12.5%, but this relationship is less evident in durum samples with high protein content (12.5–14.5%). Both protein content and kernel vitreousness can significantly affect durum milling quality. White starchy kernels (WSK) in low protein durum have a very detrimental impact on milling and pasta processing quality, but high protein content can mitigate the adverse impact of WSK on durum quality. Although protein content plays a dominant role, higher HVK might contribute positively to pasta firmness. There was no significant difference in yellow pigment content between HVK and WSK. However, pigment loss from semolina to dough was higher for WSK than HVK. Despite the difference in protein content, HVK and WSK have little difference in gluten strength. The monomeric protein was preferentially accumulated in HVK. The glutenin proteins of HVK and WSK were similar in the ratios of 1Bx/1By and HMW/LMW-GS.     

Sorghum extrusion process combined with biofortified sweet potato contributed for high iron bioavailability in Wistar rats

This study aimed to evaluate the effect of sorghum and sweet potato on the bioavailability of iron, gene expression of proteins involved in iron metabolism and the plasma antioxidant capacity in animals fed with whole sorghum grains processed by dry heat or extrusion, combined or not with sweet potato flour with high content of carotenoids. Five experimental groups were tested (n = 7): dry heat sorghum flour (DS); extruded sorghum flour (ES); whole sorghum flour + sweet potato flour (DS + SP); extruded sorghum flour + sweet potato flour (ES + SP) and positive control (FS). The evaluations included: hemoglobin gain, hemoglobin regeneration efficiency, gene expression of divalente metal transporter 1 (DMT-1), duodenal citochroma B (DcytB), ferroportin, hephaestin, transferrin and ferritin and total plasma antioxidant capacity (TAC). The ES + SP group showed higher (p < 0.05) expression of DcytB, ferroportin and hephaestin when compared to the control group. The DS group showed high (p < 0.05) expression of DMT-1 and the ES showed high mRNA expression of transferrin and ferritin. The changes in the sorghum physicochemical properties from extrusion process reduced the iron and phytate content, and increased the gene expression of proteins involved in iron metabolism, improving iron bioavailability. The combination of sweet potato and sorghum flour (dry or extruded) improved the iron capture and total antioxidant capacity, probably due to the presence of β-carotene and antioxidant compounds.     

导入AGPase基因的转基因可育水稻及其经济性状的研究

以农杆菌介导法将淀粉合成关键酶AGPase(腺嘌呤二磷酸葡萄糖焦磷酸化酶）基因导入云南地方优质水稻合系35，38，39，41，42中，获得了大量的可育转基因植株。PCR扩增检测，外源基因已稳定整合进水稻基因组中。大量的农艺性状及淀粉含量研究表明，转基因水稻种子的千粒重比对照明显提高，但总淀粉含量没有显著变化。     

砂垫层基础的设计与施工措施

在各类工程中砂垫层的作用不同,作者对软弱地基在结构设计中采用砂垫层这一措施进行了详细叙述。