Human serum albumin interaction with paraquat studied using spectroscopic methods

The interaction between herbicide paraquat and human serum albumin (HSA) was investigated by fluorescence and UV/Vis absorption spectroscopy. Paraquat can strongly quench the intrinsic fluorescence of HSA by static quenching and nonradiative energy transferring; The hydrophobic and electrostatic interactions play a major role in stabilizing the complex. The binding site number n and apparent binding constant K_A, corresponding thermodynamic parameters ΔG, ΔH, ΔS at different temperatures, were calculated. The distance r between donor (HSA) and acceptor (paraquat) was obtained according to fluorescence resonance energy transfer. The effect of paraquat on the conformation of HSA was analyzed using synchronous fluorescence spectroscopy.     

灵芝提取物对人肝癌SMMC─7721细胞DNA合成的影响

本文以人肝癌ＳＭＭＣ－７７２１细胞为材料，对水溶性灵芝提取物（ＥＧＬ）抗癌作用机理进行了研究。结果表明，ＥＧＬ能明显抑制ＳＭＭＣ－７７２１细胞的ＤＮＡ合成：ＥＧＬ在５０ｕｇ／ｍｌ到３００ｕｇ／ｍｌ范围表现出明显的剂量效应；ＥＧＬ最小有效作用剂量为１００ｕｇ／ｍｌ，最大有效作用剂量为３００ｕｇ／ｍｌ。结果提示ＥＧＬ的抗癌机理主要是通过抑制癌细胞ＤＮＡ合成而起作用的。     

国内乡村聚落景观研究综述

摘要：从聚落景观研究入手,对国内乡村聚落景观研究的基础理论、研究内容、研究方法和技术以及综述聚落景观研究的主要流派等进行介绍,指出在研究方法上侧重于定性描述,采用定量分析研究少,没有形成一个很好的古村落景观研究的评价体系。     

农杆菌介导的hIL-12基因转化马铃薯的研究

构建CaMv35S启动子驱动人白细胞介素12基因(hIL-12)的植物表达载体pBI121-hIL-12,通过三亲杂交法将重组载体导入根癌农杆菌菌株EHA105,农杆菌介导法转化马铃薯茎段,经卡那霉素抗性筛选,获得28株抗性植株。通过PCR检测,22株为阳性,从PCR阳性植株中随机选取5株,ELISA法检测茎和叶中hIL-12的表达量,其中2株与对照组相比有显著性差异(p<0.001),hIL-12的表达量分别为(3 714.0±48.8)pg/g和(3 465.0±185.0)pg/g,初步表明外源基因hIL-12已经整合进马铃薯基因组中并得到了表达,为进一步研究重组hIL-12的分离纯化和生物学活性奠定了试验基础。     

农户人力资本投资与农村贫困关系的实证研究

考察了有关人力资本投资与农村贫困关系的文献,根据1990～2007年的时间序列数据,运用向量自回归模型及其方差分解实证,研究了农户人力资本投资与农村贫困之间的关系。结果表明,农户人力资本投资与农村贫困之间存在长期的均衡关系;教育投资和健康投资均对农村贫困有显著的缓解效益,而迁移投资对农村贫困的缓解并未产生显著影响;在引起贫困波动的因素中,短期内,教育投资对贫困波动的影响大于健康投资,但在长期,健康投资对贫困波动的影响大于教育投资。基于此,提出了相关对策建议：进一步加大教育和健康投资的力度,推行义务教育和社会保障体系,加大农村合作医疗统筹力度,完善农村养老保障制度,健全农村贫困人口的培训和技术推广体系。     

人力资本与社会资本对农户非农经营选择的影响

非农经营是农户创业选择的主要活动之一.该研究运用微观调查数据对农户非农经营选择的影响因素进行Probit回归,结果表明,除物质资本和金融资本外,人力资本和社会资本也起到了显著作用.从人力资本角度看,作为家庭主要决策者的男性户主在本地非农经营中的主观能动性更强,而拥有健康的身体,受过专业的非农职业技能培训和教育,对农户选择非农经营也有推动作用;从社会资本来看,乡村干部职工户则因为视野较开阔,接触的人际范围广,拥有更多的非农经营门路和资源,得以在乡村非农经营中优先发展起来.因此,建议农村增加劳动力自雇就业的相关政策向提升农村人口职业教育水平倾斜,采取措施增加社会资本积累,以利于农产创业.     

Knockdown of HMGA2 gene inhibits TGF-β1-induced human embryonic lung fibroblast growth and collagen synthesis

AIM: To investigate the effects of high mobility group A2(HMGA2) gene knockdown on the cell viability, apoptosis, collagen synthesis and oxidative stress of human embryonic lung fibroblast (HELF) induced by transforming growth factor-β1 (TGF-β1). METHODS: The HELF were divided into blank group, TGF-β1 group,negative control (NC) group and HMGA2 siRNA(si-HMGA2) group. The protein levels of HMGA2, AKT and p-AKT were determined by Western blot. The cell viability and apoptotic rate was analyzed by MTT assay and flow cytometry,respectively. The mRNA expression of collagen I (COL-Ⅰ) and COL-Ⅲ was detected by RT-qPCR. DCFH-DA was used to detect the content of reactive oxygen species (ROS). RESULTS: Compared with blank group, the protein levels of HMGA2 and p-AKT, the cell viability, the mRNA expression of COL-Ⅰ and COL-Ⅲ in TGF-β1 group were significantly increased, but the apoptotic rate and ROS level were significantly decreased (P<0.05). Compared with TGF-β1 group, the protein levels of HMGA2 and p-AKT, the cell viability, the mRNA expression of COL-Ⅰ and COL-Ⅲ in si-HMGA2 group were significantly decreased, but the apoptotic rate and ROS level were significantly increased (P<0.05). CONCLUSION: Knockdown of HMGA2 gene expression decreases the viability and collagen synthesis, and promotes apoptosis and ROS production of human embryonic lung fibroblasts induced by TGF-β1. The mechanism may be related to down-regulation of PI3K/AKT signaling pathway.     

Ecosystem services from turfgrass landscapes

Turf is an important component of the urban and rural landscape. The natural plant formations/biomes that it mimics are the tropical savanna, the temperate grasslands (steppe and the prairies) and the tundra. Turf in a higher or lower degree provides all the ecosystem services of the other types of vegetation. Vegetation ecosystem services that have been previously emphasized include functional, aesthetical, recreational, social, and economic services as well as services related to people psychological or physical health. The purpose of this review is to gather updated information on turf ecosystem services, mainly on how they compare to other types of vegetation, or substitutes, and to suggest some future trends/areas of research.Turf has a unique role in aesthetics and, definitely, provides an irreplaceable surface for recreational sports/activities. From the available information, turf seems to have a higher potential than other types of vegetation for reducing runoff, increasing infiltration, purifying water from sediments and pollutants, controlling erosion, improving soil quality and reducing fire hazards. For the lawn owners the main turf benefits are: first the enhanced property aesthetics, second the increment in property value and third the provision of a recreation area. Turf, as all vegetation, uses water. Without the water its benefits may be reduced or annihilated. Mimicking nature may offer some solutions for saving water: summer brown lawns that green up in the fall, although losing some of the turf benefits, may be an appropriate choice where irrigation is not feasible and are worth some research. Research, should also be done on lawns using a mix of grasses and legumes: the presence of legumes may avoid N fertilization and, possibly allows for clippings removal and usage as biofuel, while keeping the soil accumulation of carbon, preventing N leaching and turning turf’s carbon footprint even more positive.     

彩色小麦近等基因系籽粒中氨基酸含量的比较分析

氨基酸是蛋白质的基本组成单位,是人体必需的重要营养物质。彩色小麦被认为是一类新型的营养保健小麦品种,但对其籽粒中氨基酸含量的研究很少。本研究首次利用高效液相色谱法比较分析了种植于三个环境中的彩色小麦近等基因系(蓝粒小麦科兴611,紫粒小麦科兴617和白粒小麦轮回亲本济麦22)籽粒中多种氨基酸的含量。结果表明,蓝粒小麦科兴611的必需氨基酸含量、非必需氨基酸含量及总氨基酸含量均显著地高于紫粒小麦科兴617和白粒亲本济麦22。紫粒小麦科兴617和白粒小麦济麦22相比,必需氨基酸含量、非必需氨基酸含量及总氨基酸含量相近,甚至低于济麦22。因此,蓝粒小麦籽粒氨基酸含量高的性状,有益于满足人类营养需求,同时可作为育种材料改良小麦品种的氨基酸含量,尤其是提高限制性氨基酸的含量,进而提高小麦的营养品质。     

公路景观构建中的人文表现

人文景观塑造是公路景观设计的重要组成部分。其在传递文化气息、表达地域特征方面具有无可比拟的先天优势,不但能够美化公路环境,也能够服务于公路行车功能,是保障行车安全的另一有效手段。笔者分析了当代公路人文景观建设中存在的问题与不足,探讨了历史人文景观在公路景观设计中的表现手段和方式,认为在塑造公路人文景观的过程中应本着尊重文化、尊重历史的态度,充分挖掘沿线所经地区的历史人文背景,提炼文化精髓,延续地域文化内涵,反对粗制滥造和凭空捏造。