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[目的]探索麝香石竹的组培脱毒技术。[方法]以麝香石竹0.3~0.4 mm长的茎尖为外植体,在分化培养基中诱导丛生芽,丛生芽进行继代培养后,将幼苗进行病毒检测,将无毒幼苗保留继续繁殖,然后将无毒的幼苗移植到生根培养基中诱导生根。[结果]不同大小的香石竹茎尖对丛生芽的诱导有较大影响,以0.3 mm茎尖为最佳。丛生芽的诱导以MS+6-BA 2.0 mg/L+IAA 2.0 mg/L、根的诱导以1/2MS+IAA 0.5 mg/L(或IBA 0.5 mg/L)为最佳培养基。田间比较试验表明,与未脱毒麝香石竹相比,脱毒麝香石竹花的颜色鲜艳,切花品质好,产花量高,花裂萼少。[结论]该研究为扩大麝香石竹的繁殖提供了技术保障。 相似文献
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香石竹(Dianthus caryophyllus),即康乃馨,又名狮头石竹、麝香石竹、大花石竹、荷兰石竹等,为石竹科石竹属的一种多年生宿根草本植物,分布于欧洲温带以及中国大陆的福建、 相似文献
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香石竹别名康乃馨,麝香石竹,石竹科,石竹属,多年生半灌木状草本.原产东亚与南欧现世界各地广为栽培.香石竹枝叶清秀,花色娇艳丰富,有红、黄、粉、白、紫、杂色等,雄蕊多瓣化,故花瓣多数,有半重瓣或重瓣品种,且芳香宜人,形状猗丽,单花花期长,露地花期为5~7月,设施栽培中可全年产花,但以每年的12月到翌年3月为最盛,且单花的... 相似文献
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An isolate of PVS, HZ00P1, was obtained from nature infected Solanum tuberosum from Hang-zhou, Zhejiang province. It was primarily identified by DAS-ELISA and host reaction tests. 3' end partial sequence of the virus isolate was cloned. Nucleic acid sequence of the cp gene and deduced cp amino acid se-quence alignments were compared with 16 other isolates registered in GenBank. The results showed that the 17 PVS isolates were divided into two groups. Among them, two Andean isolates were classified to group Ⅱ, HZ00P1 and the other 14 isolates were assigned to group Ⅰ. Compared with other PVS isolates in group Ⅰ, PVS HZ00P1 had 93.1%-98.1% and 95.9%-99.3% homologies of nucleotide sequence and deduced amino acid sequence respectively, whereas its similarities to the group Ⅱwere 81.4%-81.7% and 93.5%-93.9%. A survey of PVS occurrence in Zhejiang province was achieved by RNA spot hybridization (RSH). Among the 30 samples collected from different areas of the province, 26.7% were found to have the infection of PVS. It is concluded that PVS has become a common virus in Zhejiang province. 相似文献
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香石竹别名康乃馨、麝香石竹,属于石竹科、石竹属。为多年生草本花卉,株高60~90厘米,茎直立,多分枝。整个植株被有白粉,呈灰绿色,茎干硬而脆,节膨大。叶线状披针形,全缘,对生。花单生或2~3朵簇生于枝端,花色有大红、粉红、鹅黄、白、深红,还有玛瑙色等复色及镶边色等。香石竹花大形美,花色娇艳,具有香气,品种繁多,用于插花观赏期长而深受人们的青睐,是当今世界各国重要的鲜切花之一,也是礼仪花卉中应用最广泛的花材之一,插花、花束、花篮、捧花、胸花以及花环等高雅礼品都离不开它。它还可以露地栽培,布置花坛,也可盆栽供室内布置观赏。具体… 相似文献