意识形态观照下的唐朝佛经汉译

围绕权力和翻译的关系探究翻译中的意识形态问题,并试图证实这一论点：唐朝的佛经汉译表明翻译活动,特别是宗教文本的翻译,在翻译政策、译者和翻译策略方面受制于意识形态。通过揭示翻译文本生成中的操纵机制和政治动因,人们能够更深刻地理解翻译,特别是宗教文本翻译的本质。翻译不是纯粹的语言交际活动,而是权力控制下源语和译语的对话。     

Ultrastructural impairment of islet microvascular endothelial cells in STZ-induced type 1 diabetic mice

AIM: To investigate the ultrastructural changes of islet microvascular endothelial cells in STZ-induced type 1 diabetic mice. METHODS: BALB/c mice were randomly divided into diabetic group and control group. The expression of insulin and platelet-endothelial cell adhesion molecule-1 (CD31) in islet microvessels was detected by immunohistochemical staining. The ultrastructural changes of islet β cells and islet microvessels were observed under transmission electron microscope. RESULTS: Compared with control group, the number of islet β cells, ratio of β cells/α cells, average number of secretory granules in β cells and insulin expression area per islet in diabetic group were significantly decreased (P<0.01). Besides, diabetic group had fewer microvessels with lower expression of CD31 (P<0.01). Mitochondria in islet microvascular endothelial cells and pericytes in diabetic group were swelling. The basement membrane of islet microvessels became thicker in diabetic group (P<0.01). CONCLUSION: Islet microvascular endothelial cells were impaired in type 1 diabetic mice.     

孕穗期叶面喷素对小麦穗粒数和粒重的影响

为探讨孕穗期叶面喷素对小麦穗粒数和粒重的调控效应,于2014-2015年在河南农业大学科教示范园区进行试验,以半冬性品种豫麦49-198为供试材料,在小麦拔节后25d(4月6号),以清水为对照,叶面分别喷施硼砂(B)、6苄基腺嘌呤(6-BA)、6-BA+B、奇善宝(QSB)、双增1号(CPD),对不同喷素条件下小花败育速率和籽粒灌浆进程及其成熟后小麦穗部性状进行观察和分析。结果表明,与对照相比,不同喷素处理均能降低小花的败育速率,显著增加小麦不同部位的小穗粒数和粒重。对小麦各部位小穗粒数的促进效果,以CPD处理对小麦下、中部小穗粒数提高幅度最大,分别较对照增加了1.95个和4.85个;对不同部位小穗粒重的促进效果,也以CPD处理效果最佳,使小麦下、中和上部小穗粒重分别较对照提高了11.97%、5.87%和9.32%。     

影响共聚焦荧光法检测单细胞中蛋白表达的因素探讨

采用免疫荧光技术联合共聚焦技术研究不同培养细胞中蛋白的表达及定位.结果发现:①同样以丙酮固定293细胞,不用0.5%Triton X-100处理,着丝粒蛋白E亚型(CENP-E)在分裂期细胞核周浓聚,核内未见;而用0.5%TritonX-100处理后,CENP-E呈散点状分布于分裂期细胞的核内.②分别以甲醇、95%乙醇和4%多聚甲醛固定并以1%TritonX-100处理k562细胞,不同固定剂处理组间结果无明显差异,信号转导与转录激活因子(STAT3)均定位于胞浆.共聚焦系统PMT=511时,显示胞核染色适中,核仁染色明显,而PMT=593时,整个核呈饱和染色状,微细结构不清楚.③以2%甲醛联合0.5%TritonX-100处理平滑肌细胞,固醇调节元件结合蛋白裂解激活蛋白(SCAP)在整个细胞中呈弥散性分布,核内含量较高,高尔基体上SCAP无特异性高表达.结论:是否使用TritonX-100处理对293细胞中核蛋白CENP-E的定位具有较大影响;k562细胞对醛类固定剂及醇类固定剂无特殊选择性,图片采集时共聚焦参数PMT对蛋白定位结果也有影响;醛类固定剂和Triton联用可准确定位SCAP在平滑肌细胞中的表达.

Abstract：

The expression and location of proteins in single cells cultured under different conditions were studied with a laser scanning confocal microscope. CENP-E appeared mainly around the nucleus in HEK293 cells by fixing with acetone without 0.5% Triton X-100 treatment, but localized mainly in the nucleus when the cells were fixed with acetone plus further 0.5 %Triton X-100-PBS solution and scattered in the nucleus with condensed points. Plus l% Triton treatment, K562 cells fixed with 4% paraformaldehyde, methanol or 95 % ethanol all showed that STAT3 protein mainly distributed in cytoplasm at the edge of the cell body, the nucleus occupied a greater part of the cell body with PI staining, nucleolus staining seemed stronger under PMT= 511, but seemed the same as the other part of the nucleus under PMT=593. SCAP (SREBP cleavage-activating protein) could be detected in cytoplasm and was more accumulated in nucleus in smooth muscle cells fixed with 2 % formaldehyde and 0.5 % Triton penetrating, and Golgi apparatus were found to scatter around the nucleus. In conclusion, it is important to locate CENP-E protein in HEK293 cells with TritonX-100 by immunofluorescence microscopy. SCAP in smooth muscle cells and STAT3 expression in K562 cell can be exactly located with the combination of formaldehyde and Triton penetrating. STAT3 expression in k562 cells fixed with formaldehyde or spirits fixative has no difference.The confocal system itself, such as PMT gain, can also affect the location of proteins in single cells.     

山东省家蚕种质资源研究进展

山东省蚕业研究所保存的家蚕品种资源,从刚建所的30多个增加到现在的110多个,利用这些品种资源已育成了10对实用品种。为更好地利用种质资源,近年来进行了家蚕抗高温、抗氟及淀粉酶活性等方面的研究。     

基于CSCS方法的甘南自然植被NDVI时空分布特征研究

基于综合顺序分类系统(CSCS)模型,利用地理信息系统技术手段,模拟出甘南潜在自然植被类型图,并且结合国际地圈生物圈计划(IGBP)土地覆盖数据集,制作出甘南残存潜在自然植被分布图和残存潜在自然植被典型区分布图。在此基础上,利用2001-2010年MODIS数据分别研究了残存潜在自然植被分布区及其典型区的NDVI年度和月度变化特征。研究结果表明,1) 甘南残存潜在自然植被分布区面积达33 940 km²,占甘南总土地面积的92.53%;2) 残存潜在自然植被典型区主要分布在玛曲县、碌曲县及迭部县部分地区和夏河县北部,在舟曲、卓尼和临潭县分布较少;3) 甘南地区NDVI在2001-2010年期间都呈现出上升趋势,7种植被类型的月最大值出现在7-9月。     

龙爪槐花对异色瓢虫成虫寿命的影响

本文研究了龙爪槐花和其所含的主要糖类对异色瓢虫成虫寿命的影响。室内用龙爪槐花饲养的异色瓢虫雌、雄成虫平均寿命分别为10.96和10.83 d,显著长于龙爪槐叶上成虫寿命(雌:3.98 d,雄:3.65 d);室外龙爪槐花序上生长的异色瓢虫成虫平均寿命(雌性:14.72 d,雄性:15.46 d)也显著高于枝条上的个体(雌:3.90 d,雄:3.82 d)。补充葡萄糖、果糖、蔗糖和麦芽糖4种糖液,能显著延长异色瓢虫成虫寿命。龙爪槐花含糖量较高的花粉与花蜜是异色瓢虫成虫的适宜食物、能维持其种群发生,是花期龙爪槐上虽没有蚜虫发生、但仍有高密度异色瓢虫成虫的主要原因。     

病羊临床诊断中的消化系统检查

对羊只的系统检查是患病羊临床诊断的重要组成部分,消化系统检查是临床检查中最常见的检查。临床实践中,要特别注意对病羊的采食饮水、反刍嗳气以及各消化器官进行认真细致、有针对性的检查。     

沙薄地追施硫肥对小麦产量和品质的影响

以豫麦50（弱筋）和豫麦49（中筋）两个品种为试验材料,研究了沙薄地大田条件下追施硫肥对小麦产量和品质的影响。结果表明：拔节期追硫可增加穗粒数,提高千粒重,显著增加子粒产量;子粒硬度增加、出粉率提高,面团吸水率增加、形成时间和稳定时间延长,弱化度增加,灰分、蛋白质和湿面筋含量下降。从协调小麦产量与品质的角度出发,以少量追硫效果为好,弱筋小麦豫麦50的适宜追硫量为22.5kg/hm2,中筋小麦豫麦49的适宜追硫量为45.0kg/hm2。     

中国牧草种子国际贸易格局研究及启示

为抓住机遇快速发展中国牧草种子产业,本研究首先从国际贸易商品结构及地理分布等角度分析了中国牧草种子贸易现状,其次,探讨了中国牧草种子贸易的特点,在此基础上总结出中国牧草种子贸易的未来趋势及启示。结果显示,中国牧草种子需求量将会进一步增加、牧草种子进口量短期内将会继续保持高位,但主要牧草品种终将依赖国内市场解决供应;而当前紧迫的任务则是推动进口草种制度规范化建设、积极推进中国牧草种子专业化生产及实施科技兴草战略。