Effect of L-lysine Added in Diluent on the Fresh Semen Quality of Dorper Sheep

In order to explore the effect of different L-lysine levels added in semen dilution on the fresh Dorper sheep quality stored at liquid state,3 health Dorper ram were used to collect semen which were equal-packaged and added to solution containing different levels (0,0.1,0.2,0.3,0.4 and 0.5 g) L-lysine in 120 mL diluent and at 0,6,24,30,48,54,72 and 78 h,the sperm motility,membrane integrity and acrosome integrity were detected.The results showed that the sperm motility in groups added 0,0.1 and 0.2 g L-lysine were higher than other groups,and that in 0.4 and 0.5 g L-lysine groups decreased faster than other groups;The sperm motility in 0.5 g L-lysine group reduced to 0 at 30 h,and it reduced to 0 at 48 h in the group added 0.4 g L-lysine;The effective survival time and survival index in the group added 0.1 g L-lysine was higher than other groups (P< 0.05);The membrane integrity of 0.1 g group was the highest,that had no significant difference with control group from 0 to 54 h (P >0.05),while after 72 h the two groups had significant differences (P< 0.05).The membrane integrity in 0.4 and 0.5 g groups were the worst,and that was significant different with other groups (P< 0.05);The acrosome integrity of 0.1 g group was the highest which had no significant difference with control group (P >0.05),0.4 and 0.5 g groups were the worst,the difference between them was not significant (P >0.05),while was extremely significant difference with other groups except for 0 and 6 h (P< 0.01).The results suggested that dilution added a high concentration of L-lysine could inhibit sperm quality,when the count of L-lysine was more than 0.2 g,sperm quality was significantly decreased,adding 0.1 g L-lysine could improve Dorper sheep fresh sperm quality stored at liquid state.     

Data Collection Based on Web Services

To construct data warehouse with heterogeneous, self-governing and distributed database system is a challenge. Two problems have to be solved: one is how to collect data from heterogeneous data sources by an effective method, the other is how to clear up and change the format of the data collected from data sources. The opening and the interact-operation property of Web Services is Analyzed. A Data Collection System based on Web Services, which communicates with heterogeneous data sources with SOAP and HTTP, has been designed. A Data Collector is introduced to clear up and to transform data with composition technology. The pivotal XML technology of transforming and loading up the data are discussed.     

仿生非光滑表面形态的计算机模拟

研究发现,生物体表大都相当明显地体现着各种几何非光滑形态。生物体表非光滑形态的形成不仅与其种属遗传有关,而且与生物活性和环境等有直接的关系。不同形状的非光滑表面是为适应不同种类的土壤性质而进化成的,且有着不同的触土方式。为了研究非光滑表面形态对磨损的影响以及非光滑表面形态自身的参数变化对耐磨性的影响,本文对典型的非光滑形态进行了计算机模拟。     

烤烟上部叶叶面积相关性状的遗传及杂种优势分析

[目的]探讨不同烤烟品种(系)上部叶叶面积相关性状的配合力及其杂种优势表现,为选育上部叶开片良好的烤烟杂交种提供亲本选配依据和组合选择技术.[方法]以叶面积差异明显的8个烤烟品种(系)为亲本,采用NCⅡ遗传设计组配16个杂交组合,打顶前测定各亲本及杂交组合的自然株高和自然叶片数,打顶后7 d测定株高及顶上部往下5片烟叶的叶长和叶宽,计算叶面积,并分析上部叶叶面积相关性状的遗传及其杂种优势表现.[结果]亲本间和杂交组合间的上部叶叶面积及相关性状存在真实的遗传差异.叶面积与自然叶片数、叶长和叶宽呈显著(P<0.05)或极显著(P<0.01)正相关,相关系数分别为0.26、0.87和0.94;烤烟自然叶片数、叶长、叶宽及叶面积性状的广义遗传率高,分别为83.91%、66.33%、52.22%、50.67%,其中叶长和叶面积主要受加性效应基因的影响,而叶宽和叶片数受非加性效应基因的影响较大.对于一般配合力(GCA)和特殊配合力(SCA),自然叶片数性状中最大的分别是南江3号(3.64)和G28×南江3号(5.70);叶宽性状中最大的分别是G28(7.06)和G28×韭菜坪2号(10.91);叶面积性状中最大的分别是毕纳1号(12.54)和G28×韭菜坪2号(13.63).G28×韭菜坪2号的叶宽和叶面积性状及G28×南江3号的自然叶片数均表现为正向的超亲优势和超标优势.[结论]通过优势育种改良烤烟上部叶开片时要重视上部叶片叶宽和自然叶片数2个性状的有效利用.毕纳1号、G28和南江3号是改良烤烟上部叶开片度的优良亲本,G28×韭菜坪2号、G28×南江3号和Va116×毕纳1号的特殊配合力、超亲优势和超标优势值大,是上部叶开片较好的优良杂交组合,有较大的应用潜力.     

95%杀螟丹SP防治萧氏松茎象成虫的药效试验

试验结果表明：95％杀螟丹可溶性粉剂对萧氏松茎象成虫有良好的击倒作用。其1000倍稀释浓度对萧氏松茎象成虫的死亡率达100％，且药效在林间的持效时间可达10天。因此建议在生产上推广应用。     

小麦对缺铁胁迫的适应性反应

采用溶液培养法,比较正常供铁和缺铁胁迫下小麦的生理生化指标,同时设置加氧和不加氧2个处理。结果表明,完全营养液加氧处理的小麦株高和穗长最大,达到51.0cm和7.5cm。缺铁处理降低了小麦的籽粒长、籽粒宽、籽粒厚和穗粒数。正常供铁条件下,小麦植株叶绿素a、叶绿素b和叶绿素总含量显著高于不供铁的小麦。加氧处理的小麦叶绿素含量高于无氧处理的小麦。根际Fe3+还原酶活性在缺铁胁迫条件下达到17.3μmol·g-1·(2h)-1。缺铁处理降低了小麦植株地上部的铁素含量,并且在加氧条件下更明显。Mn、Cu这两种元素和Fe元素之间存在某种拮抗关系。     

H5N1亚型高致病性禽流感病毒A/goose/Guangdong/1/96株反向基因操作系统的建立

 A/goose/Guangdong/1/96（GSGD/1/96）是中国分离的第1株H5N1亚型禽流感病毒，它不仅是97香港感染并致人死亡的H5N1亚型流感病毒HA基因供体株，而且是中国目前已报到的H5亚型流感病毒分离株的共同祖先。本研究建立了该病毒的8质粒反向基因操作系统，并通过细胞转染成功拯救了该病毒（R-GSGD/1/96）。R-GSGD/1/96在对SPF鸡和 Balb/c小鼠的致病性方面保持了与亲本野毒(W-GSGD/1/96)一致的生物学特性，即对鸡都是高致病性毒株，R-GSGD/1/96与W-GSGD/1/96的静脉致病指数分别为2.01和2.10；救获病毒与野生病毒一样，尽管106EID50经鼻腔感染小鼠后1～2 d内能从肺脏检测到低滴度的病毒，但不能在小鼠体内成功复制。GSGD/1/96反向基因操作系统的成功建立为进一步开展中国高致病性禽流感病毒分离株的生物学特性、遗传衍化及结构与其功能关系研究奠定了基础。     

聚丙烯酰胺增加土壤降雨入渗减少侵蚀的模拟试验研究Ⅱ.侵蚀

土壤侵蚀和雨水的流失是干旱、半干旱地区制约农业发展乃至生态环境良性循环的两个主要因素。聚丙烯酰胺 (PAM ,Polyacrylamide)对土壤的性质有着明显的改进作用 ,PAM具有良好的黏结力 ,能有效改良土壤的表层结构 ,提高土壤的稳定性 ,增加土壤的入渗率 ,减少土壤侵蚀。本文试图通过人工降雨模拟实验 ,获得不同的PAM的使用量及覆盖率与土壤侵蚀量在不同的雨强、不同的坡度条件下的关系。实验表明 ,经PAM处理后土壤的入渗性能和抗侵蚀的性能都有所提高 ,PAM能明显地黏结着土壤表面的颗粒 ,形成大的团聚体 ,阻碍土壤结皮的形成 ,减少土壤侵蚀。     

高含水率木材指榫连接后主要力学性能初探

将新采伐(均在纤维饱和点以上)的马尾松(Pinus massoniana)、杉木(Cunninghamia lanceolata)、香樟(Cinnamomum camphora)指榫连接,气干后进行指榫连接处静曲强度、弹性模量、顺纹抗压强度试验,以对应的对照材为参照,探索指榫连接处的有效率。结果表明,3种高含水率木材指榫连接后,指榫连接处会较大幅度降低静曲强度,而同种木材,水平型静曲强度略大于垂直型;指榫连接处静曲强度有效率由高至低依次为:马尾松、杉木、香樟,弹性模量有效率均不86%;在一定密度范围内,木材的密度越大,指榫连接处顺纹抗压强度有效率越高。     

早熟甘蔗新品种粤糖04-252的选育

粤糖04-252是广州甘蔗糖业研究所以粤糖94-128为母本、cp72-1210为父本通过有性杂交选育的优良甘蔗新品种,该品种早熟、中至中大茎、糖分高、丰产稳产;分蘖力强、宿根性好、抗病性能好、抗逆性较强,农艺性状佳.多年多点试验结果表明,粤糖04-252公顷产蔗95.160 t,比CK1、CK2和CK3分别增产20.9％、22.6％和1.5％,与CK1和CK2差异达极显著水平;产糖14.670t/hm2,比CK1、CK2和CK3分别增产26.4％、25.2％和4.5％,差异均达极显著水平;粤糖04-252甘蔗蔗糖分含量在11月至次年1月平均达15.44％,比CK1、CK2和CK3分别提高0.67％、0.35％和0.43％,差异均达极显著水平.粤糖04-252主要农艺性状、蔗茎产量、含糖量、蔗糖分、抗逆性均优于对照种CK1、CK2和CK3,已在粤、桂、滇等主要蔗区繁殖试种.