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1.
采用ELISA(酶联免疫吸附法)测定畜产品中违禁药物克伦特罗残留量影响因子,试验结果表明,德国拜发与北京望尔生产的两种ELISA试剂盒具有操作简单、时间短、成本低、敏感度高等特点。在室温20~22℃条件下,试验制备过程中必须做到控温度、控转速、控流速及洗板过程中须注意一些事项,这样可大大提高在实际操作过程中检测的准确性。  相似文献   

2.
为探讨一种既能了解ELISA兽药残留检测是否在控又能体现检测精密度的质控图,对质控品的测定值、变异系数进行分析,提出两轴柱-线质控图,质控图中的折线可以了解检测是否在控,柱高可以体现检测精密度。该方法利用EXCELL2000电子表格绘制质控图简便、精确、快速、实用。可更准确地反映ELISA兽药残留检测实验室的质量。  相似文献   

3.
奶牛性控冻精在奶牛繁育上的应用,可以说是继人工授精、胚胎移植之后的第3次技术革命.可是在应用过程中由于性控冻精与非性控冻精相比价格要高出许多,有效精子数又相对较低(性控冻精300万每支,非性控冻精1000万每支),因此如何提高性控冻精的受胎率,就是我们目前应该急于解决的问题.现根据4年来的实践经验提出以下几点注意事项:  相似文献   

4.
情绪把控能力在某种程度上比专业技术更应得到关注,因为在演唱的高级阶段,专业技术和艺术修养的差距越来越小,而专业上的较量在某种程度上是情绪把控能力的较量.因此,文章以声乐演奏的发展现状为研究背景,分析声乐演奏过程中,影响演奏者情绪产生的原因.根据研究声乐演奏过程涉及的所有因素,探讨如何把控演奏者在声乐演奏中情感的表达,从而提升声乐演奏的质量.  相似文献   

5.
张亮 《猪业科学》2017,34(5):37-39
口蹄疫属于我国一类动物疫病,防治该病的关键是做出及时、准确的诊断。本文介绍了ELISA方法在口蹄疫诊断中的应用研究进展,传统ELISA方法包括液相阻断ELISA、间接ELISA、固相竞争ELISA、间接夹心ELISA;新型ELISA方法包括单克隆抗体ELISA、PCR-ELISA、生物素-亲和素ELISA及Dot-ELISA。随着技术的进步及养殖领域的规模化,ELISA在临床诊断和流行病学调查中将会有更加广泛的应用。  相似文献   

6.
唐文斌 《山东饲料》2013,(21):115+139
焊接温度不仅能够影响焊接质量的好坏,而且对劳动生产率的高低也有一定的影响,因此,在焊接过程中一定要注意对温度的把控。本文首先从焊接方法、焊接工艺以及手工焊接技术这单方面简单介绍了焊接技术,其次简单分析了在焊接技术中对预热、层间温度以及熔池温度的把控。  相似文献   

7.
本文的研究内容是奶牛性控精子膜蛋白双向电泳平台的建立和优化以及性控X、Y精子膜蛋白表达的差异性分析。分别对性控X精子、Y精子提取膜蛋白并进行双向电泳,用ImageMaster7.0软件对本结果进行比对分析,发现一张图谱上大约有600左右的蛋白点,其中差异蛋白点32个,在差异蛋白点中,上调蛋白19个,下调蛋白13个。说明基于双向电泳的蛋白质组学可以用于性控精子的研究,发生改变的这些差异蛋白可能在性别分化过程中发挥重要作用。  相似文献   

8.
酶联免疫吸附测定技术新进展   总被引:4,自引:0,他引:4  
酶联免疫吸附测定(Enzyme-linked immunosorbent assay,ELISA)技术是1971年由Engvall等建立的一种生物活性物质微量测定新技术,以其灵敏度高、特异性好等优点,在生命科学各领域得到广泛应用。在实际应用过程中,该技术得到不断改进,形成了多种分析方法,并且在检测的灵敏度、特异性、操作简单化以及实时、高效等方面都有很大提高,可以说ELISA是当前应用最广、发展最快的一项技术。本文仅就近几年ELISA的发展情况作一综述。 1 常规ELISA常规ELISA试验方法如间接法、双抗夹心法及竞争法或阻断法仍然被广泛用于诊断各种病毒、…  相似文献   

9.
<正>近几年,畜产品质量安全意识与日俱增,动物疫病形势复杂化,基层动物疫病防控部门做好动物疫病筛查工作显得尤为重要。酶联免疫吸附试验(ELISA)因其具有操作简便、灵敏度高、特异性强等优点被广泛应用于血清、尿、饲料等样品筛检中。ELISA试验看似简单但在实际的操作中,影响测定结果的因素较多,稍有不慎便会导致弱板、白板、花板等不正常现象或假阳性、假阴性等异常结果。笔者对ELISA测定过程中  相似文献   

10.
用荷斯坦奶牛性控精液和常规精液对奶牛受胎率的影响进行试验。通过比较分析,结果显示,采用性控精液人工授精的情期受胎率为59%,采用常规精液人工授精的情期受胎率为74%。试验表明,性控精液组的受胎率明显低于常规精液组。其主要原因是性控精液在分离过程中,精子受到不同程度的损伤,使精子的存活时间缩短,从而增加了未受精率,导致奶牛的受胎率下降。  相似文献   

11.
本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。  相似文献   

12.
用硝酸和高氯酸消化蜂蜜,使硒游离出来,在微酸性环境下,硒和2,3-二氨基萘(DAN)生成有较强荧光的物质,用环己烷萃取,在激发波长378nm,荧光波长518nm处测定其荧光强度。蜂蜜中硒含量范围:0.10~0.82μg/g。表明:蜂蜜应视为天然富硒营养品。  相似文献   

13.
乳酸杆菌益生作用机制的研究进展   总被引:2,自引:0,他引:2  
乳酸杆菌作为益生菌广泛用于人和动物。本文综述了乳酸杆菌改善宿主健康的机制。乳酸杆菌可通过产生抗菌物质如乳酸、过氧化氢、细菌素,或者通过竞争营养或肠道黏附位点来抑制致病菌;通过诱导黏附素的分泌或阻止细胞凋亡而增强肠道的屏障功能,从而保护肠道。文章重点讨论了乳酸杆菌表面成分(表面蛋白、脂磷壁酸和肽聚糖)与肠道受体(C型凝集素受体、Toll样受体和 Nod样受体),阐述了他们结合后启动免疫调节信号,调控肠道免疫功能以发挥改善健康作用的机制。  相似文献   

14.
以国际标准强毒R株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离MG,并收集感染鸡所产蛋分离MG。结果表明,人工感染48小时后上、下呼吸道及肺已被全面感染,96小时气囊已被感染,120小时输卵管已能分离到MG,卵巢始终分离不到MG。人工感染鸡自144小时便能在其所产蛋中分离出MG。药物治疗能在72小时内消除感染,油乳剂苗则需24天后逐渐降低蛋内MG分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内MG,但以研制的种蛋浸泡剂药浴效果为最好。  相似文献   

15.
在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。  相似文献   

16.
Reasons for performing study: Clinical experiences indicate that centesis of the lateral compartment of the femorotibial joint is often unsuccessful. Objective: To determine the accuracy of 2 published and 2 unpublished techniques of centesis of the lateral compartment of the femorotibial joint. Hypothesis: It was hypothesised that a drug can be administered into the lateral compartment of the femorotibial joint via a diverticulum of this joint that surrounds the medial aspect of the long digital extensor tendon and that this technique is more accurate than described techniques of centesis of this compartment. Methods: Twenty‐four stifles of 12 horses were divided equally into 4 groups and a radiocontrast medium injected into the lateral compartment of the femorotibial joint of each group using a hypodermic needle inserted: 1) caudal to the lateral patellar ligament and proximal to the tibial plateau, 2) caudal to the long digital extensor tendon and proximal to the tibial plateau, 3) between the long digital extensor tendon and bone of the extensor groove of the tibia or 4) directly through the long digital extensor tendon until it contacted bone. Twelve veterinary students who had no experience using any of these techniques performed the injections. Accuracy of each technique was determined by examining radiographs obtained after injecting the contrast medium. Results: The most successful technique for arthrocentesis was by inserting a needle through the long digital extensor tendon. This approach was successful in all attempted cases, whilst other techniques had lower rates of success. Conclusions: The lateral compartment of the femorotibial joint can be accessed accurately by inserting a needle through the long digital extensor tendon as it lies within the extensor groove. Other techniques may not be as accurate for clinicians inexperienced in arthrocentesis of the lateral compartment of the femorotibial joint.  相似文献   

17.
本试验旨在研究两种绿头鸭(白羽绿头鸭和绿头鸭)肌肉营养组成和价值,为绿头鸭肉产品的开发利用提供基础数据和理论依据。选择白羽绿头鸭和绿头鸭60只(每个品种各30只,公母各半),依照国家标准测定胸肌粗蛋白质、粗脂肪、胆固醇含量及氨基酸、脂肪酸、微量元素和维生素的组成和含量,并对胸肌进行氨基酸评价及脂肪酸营养价值评价。结果显示,白羽绿头鸭肌肉粗蛋白质含量显著低于绿头鸭(P<0.05);两种绿头鸭肌肉中均检测到17种含量大于0.01%的氨基酸,其中白羽绿头鸭肌肉苏氨酸、组氨酸、丝氨酸和脯氨酸含量显著高于绿头鸭(P<0.05),赖氨酸、谷氨酸和精氨酸含量显著低于绿头鸭(P<0.05);检测到13种含量均大于0.01%的脂肪酸,其中白羽绿头鸭硬脂酸和油酸含量显著低于绿头鸭(P<0.05);检测到9种矿物质元素(钠、镁、钾、钙、锰、铁、铜、锌和硒),两种绿头鸭差异不显著(P>0.05);检测到8种维生素,白羽绿头鸭肌肉维生素B1含量显著高于绿头鸭(P<0.05),维生素D和维生素E含量显著低于绿头鸭(P<0.05)。两种绿头鸭肌肉氨基酸比例接近世卫组织推荐的理想模式,富含人体所需的矿物质元素和维生素,具有广阔的开发利用前景。  相似文献   

18.
This experiment was aimed to study the nutritional content and value of the muscles of two mallards (White feather mallard and mallard),and provide basic data and theoretical basis for the development and utilization of mallard meat products.60 White feathered mallards and mallards (30 for each breed,half of male and female) were chosen,and the composition and content of pectoral muscle crude protein,crude fat,cholesterol,amino acids,fatty acids,trace elements and vitamins were determined according to national standards and the nutritional values of muscle were evaluated.The results showed that the content of crude protein in muscle of White feather mallard was significantly lower than that of mallard (P<0.05).17 kinds of amino acids with contents higher than 0.01% were detected in the muscles of the two kinds of mallards,among which the contents of threonine,histidine,serine and proline in the muscles of the White feather mallard were significantly higher than those of mallard (P<0.05).The contents of lysine,glutamate and arginine were significantly lower than those of mallard (P<0.05).13 kinds of fatty acids with contents higher than 0.01% were detected,and the contents of stearic acid and oleic acid were significantly lower than those of the mallard.9 mineral elements (sodium,magnesium,potassium,calcium,manganese,iron,copper,zinc and selenium) in two kinds of mallards were detected,and there was no significant difference between the two kinds of mallards (P>0.05).8 kinds of vitamins were detected,and the content of vitamin B1 in muscle of White feather mallard was significantly higher than that of mallard (P<0.05),but the contents of vitamin D and vitamin E were significantly lower than that of mallard (P<0.05).The ratio of amino acids in muscle of two kinds of mallards was close to the ideal model recommended by WHO,which was rich in mineral elements and vitamins for human body,and had a broad prospect of development and utilization.  相似文献   

19.
应用光镜和透射电镜技术,观察了3~6月龄比格犬甲状腺的显微和超微结构。结果表明,比格犬甲状腺实质由滤泡和滤泡旁细胞构成。滤泡呈圆形或椭圆形,直径20.22~220.00μm,平均90.80μm;由单层立方上皮细胞围成,细胞高度3.04~7.11μm,平均5.18μm,电镜下可见功能状态不同的两型滤泡上皮细胞;滤泡旁细胞很多,直径4.40~8.82μm,平均6.23μm,位于滤泡之间或镶嵌于滤泡上皮细胞之间,也可聚集在一起形成滤泡样结构,胞质内含有大量的分泌颗粒,电镜下也可见两种类型的滤泡旁细胞。  相似文献   

20.
A retrospective review of records of 205 cats with histologically confirmed disease of the spinal cord was performed to identify the prevalence of disease in this nonrandomly selected population of cats. Clinical records were reviewed, and age, duration of neurologic illness, and clinical and histopathologic findings in cats with spinal cord disease were abstracted. Disease processes were classified into 7 categories and 23 groups. The most common diseases affecting the spinal cord of cats were feline infectious peritonitis (FIP), lymphosarcoma (LSA), and neoplasia of the vertebral column secondarily affecting the spinal cord. Information on age, onset and duration of clinical signs, and lesion localization at the postmortem examination in cats belonging to the 7 categories of disease were analyzed to create a practical list of differential diagnoses. Cats were also subcategorized into 3 groups based on their age at death. FIP was the most common disease of cats younger than 2 years of age. LSA and vertebral column neoplasia were the most common diseases affecting cats between 2 and 8 years of age. Vertebral column neoplasia was the most common disease affecting cats older than 8 years of age. Results of this histopathologic study showed that FIP and LSA were the most common disease processes affecting the spinal cord of cats. However, at least 21 other groups of diseases and their relative prevalence were identified.  相似文献   

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