猪支原体肺炎的诊治报告

猪支原体肺炎属国家二类动物疫病,是由猪肺炎支原体引起猪的一种慢性呼吸道传染病,俗称猪气喘病,亦称猪地方流行性肺炎。临床上以咳嗽、气喘为主要症状,特征性病变是在肺的心叶、尖叶、中间叶及膈前下缘有实变区(融合性支气管肺炎病灶),肺门淋巴结肿大。2004年6月11日,宁蒗县某     

一起猪气喘病的诊疗方案

<正>猪气喘病,又称猪地方流行性肺炎或猪支原体肺炎。是猪的一种高度接触性慢性呼吸道传染病,广泛分布于世界各地,其主要症状是咳嗽和气喘,生长发育缓慢,病变特征是融合性支气管肺炎,肺尖叶、心叶、中间叶和膈叶前缘呈"肉样"或"虾肉样"实变。本病在暴发流行的初期或饲养管理不善,     

大型猪场猪气喘病的综合防制

猪气喘病,又称猪地方流行性肺炎或猪支原体肺炎。是猪的一种高度接触性慢性呼吸道传染病,广泛分布于世界各地。其主要症状是咳嗽和气喘,生长发育缓慢,病变特征是融合性支气管肺炎,肺尖叶、心叶、中间叶和膈叶前缘呈"肉样"或"虾肉样"实变。本病在暴发流行的初期若饲养管理不善,     

猪气喘病的中西医疗法

猪气喘病,又称猪地方流行性肺炎或猪支原体肺炎。是猪的一种高度接触性慢性呼吸道传染病,广泛分布于世界各地。其主要症状是咳嗽和气喘,生长发育缓慢,病变特征是融合性支气管肺炎,肺尖叶、心叶、中间叶和膈叶前缘呈"肉样"或"虾肉样"实变。如有继发性病原体感染,可造成大批死亡,猪肺炎支原体,对外界抵抗力不强,常用化学消毒剂即可杀灭。猪肺炎支原体对青霉素及磺胺类药物不敏感,对四环素类、泰妙菌素、泰莫灵敏感。     

种猪场猪喘气病的综合防治

<正>猪喘气病是由猪肺炎霉形体感染引起的高度接触性慢性传染性疾病。主要症状为咳嗽和气喘。病变的特征是融合性支气管肺炎,于尖叶、心叶、中间叶和膈叶前缘呈"肉样"或"虾肉样"病变。患猪长期生长发育不良,饲料转化率低。     

黄颡鱼感染小肠结肠炎耶尔森氏菌的组织病理学观察

为探讨小肠结肠炎耶尔森氏菌GM2402株对黄颡鱼的致病性,本试验采用人工回感试验测定其半数致死浓度(LC₅₀),同时采用组织病理学方法探讨该菌对黄颡鱼组织的影响.结果表明:回感18 h后,发病黄颡鱼腹部肿大,胸鳍、腹鳍基部出血,肛门红肿,其LC₅₀为3.0×10^6.2 CFU/mL.组织病理学观察肝细胞肿大、排列紊乱、广泛空泡变性;肾小球萎缩,肾小管上皮细胞肿大;脾脏组织结构疏松充满大量红细胞;鳃小片轻度水肿充血.超微结构观察结果显示肝脏和肠道细胞内线粒体均出现不同程度的肿胀,嵴断裂囊泡化.本试验结果表明小肠结肠炎耶尔森氏菌GM2402株对黄颡鱼有较强的致病性,可导致鱼体多个组织出现病变.     

集约化猪场猪喘气病的综合防制

猪喘气病是由猪肺炎支原体引起的一种接触性、慢性呼吸道传染病 ,在猪群中可造成地方性流行。主要特征是喘气、咳嗽 ,呈腹式呼吸 ,体温及食欲变化不大而生长发育迟缓。剖检可见肺有不同程度的水肿和气肿 ,两肺的尖叶和心叶呈对称性、融合性支气管肺炎病变。在病肺的尖叶、心叶、中间叶和隔叶的前缘呈肉样或虾肉样实变。肺门和纵膈淋巴结明显肿大、质硬、灰白色切面多汁 ,其他内脏一般无明显变化。在诊断本病时应注意继发其他猪病如猪流感、猪肺疫和猪肺丝虫病等引起的混合感染。本病发病率高 ,不同品种、年龄、性别的猪一年四季均可发病。寒…     

珠三角地区几例伪狂犬病猪的组织病理学观察

近年来猪伪狂犬病在珠三角及其周边地区广为流行,已成为猪的主要传染病之一。剖检可见各器官有不同程度的出血、坏死。镜检脾小体和淋巴细胞数目减少;肝细胞肿胀,坏死,肝小叶结构紊乱;肺泡腔代偿性增大,充满蛋白质性水肿液;肾小管上皮细胞肿胀、崩解脱落,肾小球肿大。     

猪的衣原体性流产

１９９０年一个养猪场发生了猪的流行性流产。妊娠末期的流产胎儿皮浮肿且伴有全身性出血斑，剖检后发现血样胸不及超量腹水，肝脏肿大，心脏上存在出血斑及淋巴节肿大。妊娠中期至后期流产的胎儿无明显变化。从病理组织学上可以认定，胎儿患有非化脓性脉络膜脑膜炎，肝细胞变性及坏死，心内膜炎，心外膜炎和血管炎等多种病症。通过免疫组织学检查，确认在上述病变中有衣原体浸入单核细胞，肝细胞及胎膜上皮细胞形成的原生小体。从流     

猪轮状病毒的致病性试验

为了对引起北京地区仔猪腹泻的轮状病毒进行致病性研究,购买未采食母乳的初生仔猪6头,分成2组,试验组3头仔猪口服并皮下注射三代细胞病毒1 m L,观察其临床症状;分别在感染前及感染后24、48、72、96 h等采集肛门拭子,RT-PCR检测病毒抗原,观察排毒情况;取感染5 d后的小肠、肝、脾等组织,观察病理学变化。结果显示:试验仔猪感染12 h,肛门拭子即可检测到轮状病毒,一直持续到第5天。组织切片观察发现,仔猪小肠上皮细胞及周围绒毛发生变性,上皮细胞变圆、肿胀、核肿大,细胞边缘不规则。变性细胞从基质脱落至管腔,萎缩绒毛被扁平上皮细胞覆盖,固有层有少量细胞碎片。     

犊牛磺胺中毒的病理形态学观察

为了解犊牛磺胺中毒的病理形态学变化特点,对2头因磺胺中毒而死亡的犊牛进行了详细的病理学研究.结果表明,犊牛磺胺中毒的病理性损伤主要发生于肾脏和肝脏.宏观检查可见,肾脏肿胀,色泽变淡,皮质增宽,肾盂有多量豆腐渣样物质.肝脏肿胀,淡橘红色,质地脆易碎.微观检查可见,近曲小管上皮细胞肿胀,核浓缩,染色不良,大量上皮细胞脱落....     

猪不同生长阶段甲状腺结构的形态计量学研究

为观察猪不同生长发育阶段甲状腺的形态学变化,本研究选用大约克夏猪,分别于初生、体质量为20、40、80和100kg时屠宰,测定甲状腺质量、长度、宽度、增殖细胞数量以及血清中甲状腺素的表达,并对猪的甲状腺石蜡切片进行组织学观察,应用Nikon NIS-Elements Documentation图像分析系统对甲状腺滤泡腔以及滤泡上皮细胞进行形态计量学指标的测量,并在不同生长阶段进行比较分析。结果表明,随着猪的生长发育,甲状腺增殖细胞的数量、甲状腺素的表达以及滤泡腔数密度、上皮细胞高度均逐渐降低;滤泡腔截面积及其等效直径、滤泡腔截面积密度逐步升高;而上皮细胞核截面积及其等效直径、上皮细胞核数密度变化规律不明显。相关分析表明,血清中FT3的表达与甲状腺宽度存在显著的负相关、与上皮细胞高度呈显著正相关;FT4、TT4的表达与体质量、甲状腺质量、甲状腺长度、甲状腺宽度、滤泡腔截面积、滤泡腔截面积等效直径存在显著负相关,与上皮细胞高度以及上皮细胞核数密度存在显著正相关。上述形态学测量结果为深入研究猪甲状腺在不同生长发育阶段所发生的形态学变化提供了准确的定量资料。     

利用单抗免疫组化技术检测PCV-2 WH株在人工感染猪组织中的分布

将猪圆环病毒2型(PCV-2)WH株通过颈部肌注和滴鼻感染26日龄PCV抗体阴性仔猪,于攻毒后28 d剖杀,采集相关的神经组织、消化器官及免疫器官进行免疫组化SABC法检测、病理学观察及PCR检测。免疫组化结果显示,在攻毒猪的大脑和小脑、胃、十二指肠、淋巴结、脾脏、扁桃体、肺、肾、肝存在阳性信号,心脏无阳性信号。阳性信号主要位于淋巴细胞和巨噬细胞的胞浆中,偶尔出现在细胞核内和上皮细胞中。免疫组化结果与病理学变化和PCR检测基本相符。结果表明,本试验建立的免疫组化SABC法,可用于定位PCV-2 WH株在体内不同组织器官中的分布情况。     

Effects of short-term sodium chlorate exposure on pigs

The present study evaluated the effects of exposure to different doses of sodium chlorate in 10-week-old pigs. Twenty pigs were divided into four equal groups and treated with different doses of sodium chlorate: 0, 125, 250 and 500 mg kg-1 body weight per day via the drinking water for 7 consecutive days. The results showed a significant decrease (P < 0.05) in red blood cell and white blood cell counts, packed cell volume, haemoglobin, blood urea nitrogen (P < 0.001) and creatinine levels, and an increase in aspartate aminotransferase and alanine aminotransferase (P < 0.05) activities in swine administered sodium chlorate at a dose of 500 mg kg-1 body weight per day. The histopathological study revealed increased numbers of vacuoles in the convoluted tubules, tubular necrosis and degeneration of the renal tubular epithelial cells, depletion of nuclei and lobular necrosis of the liver in all pigs treated with sodium chlorate at 500 mg kg-1 body weight per day. Thus, 7-day administration of sodium chlorate at 500 mg kg-1 body weight per day to pigs affects the liver and kidney tissues as well as the haematologic and serum biochemical parameters.     

Sites of replication of a porcine respiratory coronavirus related to transmissible gastroenteritis virus

A porcine respiratory coronavirus (PRCV) was inoculated by aerosol into nine hysterectomy-derived and colostrum-deprived pigs at the age of one week. They were killed at different times after inoculation and tissues were sampled for virus isolation and immunofluorescence. Results indicate that virus replicated to high titres in the respiratory tract. Replication mainly occurred in alveolar cells but also in epithelial cells of nasal mucosa, trachea, bronchi, bronchioli, in alveolar macrophages and in tonsils. After primary replication in the respiratory tract, viraemia occurred. Virus also reached the gastrointestinal tract after swallowing. Subsequently, PRCV was observed to replicate in the ileum. The infection spread within a few days from the ileum to the duodenum. Replication in the small intestine remained limited to a few cells located in or underneath the epithelial layer of villi and, or, crypts. The cell type could not be identified. Virus was isolated from mesenteric lymph nodes in all pigs, but immunofluorescence was not observed. Results show that small changes in molecular structure between transmissible gastroenteritis virus and PRCV resulted in important changes in host cell tropism.     

Ulex europaeus agglutinin 1 lectin histochemical staining of dog kidney and bile duct epithelial cells

Ulex europaeus agglutinin 1 lectin-binding histochemical staining of dog liver and kidney failed to demonstrate staining of endothelial cells. Staining of renal tubular epithelial cells occurred in different segments of the nephron. Staining of some epithelial cells occurred in larger intrahepatic bile ducts.     

Effects of Long-term Cyanide Ingestion by Pigs

Animal performance and health status are adversely affected by long-term cyanide ingestion; however, the effects of cyanide ingestion by pigs have not been fully determined. The aim of the present study was to determine the effects of prolonged exposure to different doses of potassium cyanide (KCN) in growing-finishing swine. Twenty-four pigs, 45 days of age, were divided into four equal groups and treated with different doses of KCN: 0, 2.0, 4.0 or 6.0 mg per kg body weight per day for 70 consecutive days. The results showed a significant alteration in thiocyanate, creatinine and urea levels and in alanine aminotransferase activity of swine dosed with 4.0 and 6.0 mg/kg/KCN. Thyroid weight was significantly increased in those pigs from 4.0 mg/kg KCN group, but no change in cholesterol, triiodothyronine or thyroline levels were observed. Body and carcase weights, body weight gain, and bacon thickness were not affected by KCN treatment. The histopathological study revealed increased numbers of vacuoles in the colloid of thyroid follicles, degeneration of cerebellar white matter and Purkinje cells, degeneration of renal tubular epithelial cells, caryolysis and pyknosis in hepatocytes, and disturbance of the normal lobular architecture of the liver in all treated pigs. Thus, long-term administration of KCN to swine affects several tissues and could adversely affect animal production.     

Tonsillar changes in pigs given pseudorabies (Aujeszky's disease) virus

All of the eight 5-day-old pigs orally given pseudorabies (Aujeszky's disease) virus developed tonsillitis. The initial changes occurred in the subepithelial area between the lymphoid nodule and the crypt epithelium, showing a characteristic pattern of necrosis. The necrosis became more severe and gained access into the lymphoid nodule and crypt epithelium. Coincident with the histopathologic changes, numerous specific immunofluorescences were detected, first in the nucleus and in some parts of the cytoplasm of cells distributed in the subepithelial area. The fluorescence subsequently spread into adjacent lymphoid nodules and crypt epithelial cells. Ultrastructurally, many enveloped virus particles were detected in the center of the necrosis. Thereafter, the crypt epithelial cells also underwent degeneration, and a small number of virus particles were detected in the nucleus of the degenerating epithelial cells. In the more advanced stage, the enveloped virus particles were discharged into the crypt lumen.     

Regional Ileitis in Pigs: Morphological and Pathogenetical Aspects

Twenty-seven weaned pigs with a wasting appearance were investigated. From a morphological point of view the pigs were divided into three groups. In the group of pigs with macro-scopical signs of regional ileitis, affected tissue showed substantial epithelial proliferation, and electron microscopic studies revealed the presence of microorganisms within the cytoplasm of the epithelial cells. There was a loss of, or only faint, enzymatic activity of alkaline phosphatase in the mucosal epithelium. In another group of five pigs there was no, or only slight, light microscopical signs of regional ileitis but presence of intracellular microorganisms. The enzymatic activity of the ileal epithelium was low. Low enzymatic activity of the ileal epithelium was observed in a third group of wasting pigs, which had no histological or electron microscopical signs of regional ileitis.