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1.
概述了苦马豆素的来源和理化特性的研究情况,从薄层色谱(TLC)、气相色谱(GC)、高效液相色谱(HPLC)、酶分析法、荧光光谱和离子抑制色谱等几个方面介绍了苦马豆素定性、定量检测方法的研究进展,并对各种检测方法的优缺点进行了比较。  相似文献   

2.
用薄层色谱扫描法对土霉素(OTC)回收率进行了测定。经试验,发现波长在280nm处进行荧光单波长线性扫描时,OTC有最大吸收峰,其回收率可达94.49%。  相似文献   

3.
采用高效液相色谱法(HPLC),分离柱选用ODS色谱柱,以纯水为洗脱液,用示差折光检测。测定了饲料添加剂和功能食品中低聚糖的含量,即蔗糖、水苏糖和棉子糖。本法具有流动相价廉、无污染、快速简便和稳定性好的特点,色谱柱使用寿命长。  相似文献   

4.
本研究系作者在完成中国兽药典委员会下达的“木香槟榔散薄层色谱复核试验”任务时所人的研究,该研究结果现已收入《中华人民共和国兽药典》二部(2000年版)P.346“木香槟榔散”的[鉴别]项下。在本项研究中,作者对处方中大黄,黄连,黄柏三味药材进行了薄层色谱试验,其方法选取了《中华人民共和国药典》一部(95年版)P.415“木香槟榔丸”中的[鉴别](3),(4)和P.16大黄项下的[鉴别](3),P.273黄连项下的[鉴别](3)进行,试验中采用处方样品,缺味阴性样品与对照药材和对照品进行比较,试验结果表明,木香槟榔散中大黄和黄连的薄层色谱均可以采用《中华人民共和国药典》一部(95年版)“木香槟榔丸”中大黄和黄连的薄层色谱方法,只改变其取样量,其余不变。  相似文献   

5.
本试验采用乙醚作萃取剂,提取牛乳中的氨基酚、硝基苯胺和硝基酚,用高效液相色谱法同时对三种物质进行检测。高效液相色谱条件:固定相为C18反相色谱柱;流动相为0.008mol/LKH2PO4溶液:色谱纯甲醇(60:40);离子对为四丁基溴化胺(终浓度为0.003mol/L);柱温25%;紫外检测器检测波长270nm;流速1.0mL/min。结果表明,该方法能够准确地对牛乳中的氨基酚、硝基苯胺和硝基酚进行定性和定量检测。  相似文献   

6.
本文建立了酸奶和液体奶中对羟基苯甲酸酯类防腐剂中的对羟基苯甲酸乙酯(EP)和对羟基苯甲酸丙酯(PP)的高效液相色谱(HPLC)分析方法。样品用甲醇溶解后用超声波进行提取,采用紫外检测器检测,检测波长256nm,2种防腐剂在10min之内完全分离。各组分的回收率为96.9%~102%,检出限为1mg/kg,线性范围为1.0—40ug/mL。  相似文献   

7.
应用经验证可靠的ELISA方法,对进口猪产品进行莱克多巴胺残留快速筛选检测,对ELISA检测阳性的样品采用色谱质谱联用分析方法进行确证检测。1330份猪产品的检测结果显示,采用ELISA方法检出阳性率达11.4%(152/1330);对ELISA阳性样品采用高效液相色谱-串联质谱(HPLC—MS/MS)或气相色谱-质谱方法(GC—MS)确证,阳性率达90.8%(138/152)。检测工作表明,综合运用快速筛选检验技术与确证检测技术可实现快速准确检测动物产品中莱克多巴胺残留,提高进出境检验工作效率。  相似文献   

8.
应用经验证可靠的ELISA方法,对进口猪产品进行莱克多巴胺残留快速筛选检测,对ELISA检测阳性的样品采用色谱质谱联用分析方法进行确证检测。1330份猪产品的检测结果显示,采用ELISA方法检出阳性率达11.4%(152/1330);对ELISA阳性样品采用高效液相色谱-串联质谱(HPLC—MS/MS)或气相色谱-质谱方法(GC—MS)确证,阳性率达90.8%(138/152)。检测工作表明,综合运用快速筛选检验技术与确证检测技术可实现快速准确检测动物产品中莱克多巴胺残留,提高进出境检验工作效率。  相似文献   

9.
采用细胞自溶法对影响解脂假丝酵母33M(cand id a lip oly tica)细胞中神经酰胺含量的条件进行了研究。分别应用薄层色谱(TLC)方法和高效液相色谱-蒸发光散射器(HPLC-ELSD)对其进行定性和定量分析,结果表明:解脂假丝酵母的最佳自溶条件为:自溶时间24 h,pH为8.0,自溶温度为50℃。  相似文献   

10.
文章旨在建立同时测定渔用饲料中氟苯尼考及氟苯尼考胺残留量的同位素稀释-高效液相色谱串联质谱法。以氯霉素-D5和氟苯尼考胺-D3位内标,样品用氨化乙酸乙酯提取,混合型阳离子交换固相萃取柱(MCX)净化,在金刚烷基色谱柱上分离,采用LC-MS/MS选择多反应监测(MRM)模式进行定性、定量分析。结果表明:氟苯尼考及氟苯尼考胺的检出限(LOD)为0.2和0.5μg/kg,定量限(LOQ)为1.0和2.0μg/kg,检测结果的相对标准偏差为2.04%~12.1%(n=6),加标回收率达到64.3%~97.0%。该方法具有比较高的重现性和选择性,在渔用饲料中氟苯尼考及氟苯尼考胺的残留测定中具有很好的应用前景。 [关键词]高效液相色谱-串联质谱法|同位素稀释法|氟苯尼考|氟苯尼考胺|渔用饲料  相似文献   

11.
不同沼液灌溉强度对土壤和渗滤液的影响   总被引:3,自引:0,他引:3  
采用盆栽试验研究不同强度施用沼液种植杂交狼尾草对环境的影响.试验按所施用沼液比例不同设7个处理,每个处理5个重复.盆栽试验采用20 L的塑料桶,桶底钻孔以收集渗滤液;灌溉间隔5 d,每次灌溉后3 h收集渗滤液.盆栽采用水稻土和株高、根系相近的杂交狼尾草苗.结果表明:随着沼液灌溉强度的提高,渗滤液和盆栽土中三氮(总氮、氨氮、硝氮)含量升高,但再高灌溉强度可导致硝氮下降;按本试验条件,合适的沼液灌溉强度是控制沼液不超过50%,渗滤液总氮、氨氮、硝氮分别稳定在52.99~57.87 mg/L、52.66~52.78 mg/L和3.33 mg/L以内.以上说明,沼液灌溉杂交狼尾草存在一个合适灌溉强度,这与一定时间内的沼液灌溉量及其养分含量有关,并受施用气候、土壤条件、植被发展影响.  相似文献   

12.
为了有效控制泰拉霉素原料及注射液的产品质量,研制了泰拉霉素国家对照品。采用精制后的泰拉霉素为原料进行本批对照品的研制,原料分装后进行质量检测及均匀性评价,并标定含量。采用红外分光光度法、费休氏水分测定法等对其进行理化分析,利用高效液相色谱法进行有关物质测定及均匀性检验,采用质量平衡法进行标准物质含量计算,并通过差示扫描量热法对质量平衡法的准确性进行验证。研制的泰拉霉素国家对照品的含量为98.3%,采用多种不同方法对本品进行定性与定量研究,确保了国家标准物质标定结果的准确性。本对照品可用于泰拉霉素原料及注射液的鉴别与含量测定,对于提高和保证兽药产品质量具有重要意义。  相似文献   

13.
采用盆栽试验研究不同强度施用沼液种植杂交狼尾草对环境的影响。试验按所施用沼液比例不同设7个处理,每个处理5个重复。盆栽试验采用20 L的塑料桶,桶底钻孔以收集渗滤液;灌溉间隔5 d,每次灌溉后3 h收集渗滤液。盆栽采用水稻土和株高、根系相近的杂交狼尾草苗。结果表明:随着沼液灌溉强度的提高,渗滤液和盆栽土中三氮(总氮、氨氮、硝氮)含量升高,但再高灌溉强度可导致硝氮下降;按本试验条件,合适的沼液灌溉强度是控制沼液不超过50%,渗滤液总氮、氨氮、硝氮分别稳定在52.99~57.87 mg/L、52.66~52.78 mg/L和3.33 mg/L以内。以上说明,沼液灌溉杂交狼尾草存在一个合适灌溉强度,这与一定时间内的沼液灌溉量及其养分含量有关,并受施用气候、土壤条件、植被发展影响。  相似文献   

14.
Objective— To determine the number, size, and configuration of ligaclips most resistant to tensile forces when applied to cellophane bands.
Study Design— In vitro mechanical evaluation.
Sample Population— Single-layer and triple-layer cellophane bands, 9.0 and 11.5 mm ligaclips.
Methods— Triple-layer bands were secured with a different number (2–5), size (9.0 or 11.5 mm) or configuration (linear or alternating placement) of ligaclips and mechanically tested. Force-deformation curves were generated and yield load in Newtons (N) was determined for each variable. A 3-way analysis of variance with post hoc Tukey's tests was used for statistical comparisons. Yield load for single-layer and triple-layer bands secured with 4 alternating 11.5 mm ligaclips was compared using a paired-sample (independent) t-test with P <.05 considered significant.
Results— Mean yield load increased as the number of ligaclips applied increased, but this effect began to plateau after application of the 4th clip. Mean yield load for 11.5 mm ligaclips was significantly higher than for 9.0 mm ligaclips ( P <.001) and for the alternating configuration compared with the linear configuration ( P <.001). Yield load for 4 alternating 11.5 mm ligaclips applied to triple-layer cellophane bands was significantly greater than the same configuration applied to single-layer cellophane bands ( P <.001).
Conclusion— 11.5 mm ligaclips applied in an alternating configuration and on triple-layer cellophane provided most resistance to tensile forces. The resistance to tensile forces increased significantly as the number of ligaclips applied increased from 1 to 4/band.
Clinical Relevance— Surgeons should be aware that the number, size, and configuration of ligaclips and cellophane thickness affect their resistance to tensile forces.  相似文献   

15.
新型激素制剂对母猪诱导发情效果研究   总被引:1,自引:0,他引:1  
应用PX-700制剂对乏情母猪进行了诱导发情研究,结果表明:700IU是PX-700制剂诱导乏情母猪发情的适宜剂量;而该制剂对断奶后45d以上的乏情母猪诱导发情率较低,但其情期受胎率不受影响;PX-700制剂对诱导发情后母猪的情期受胎率、产仔数及仔猪出生重均无影响。  相似文献   

16.
Sheep cold stressed for 10.5 h had plastic coats applied then the cold stress was continued. In 9 of 10 sheep (test group) showing hypothermia at the time coats were applied, body temperatures had returned to near normal 2.5 h later and to normal in 13.5 h. One sheep severely hypothermic before the coat was applied did not improve and was killed after 2.5 h. Sheep that were cold stressed without coats (wet controls) developed marked hypothermia and were killed between 10.5 h and 21 h. Rectal temperatures remained normal in controls that were cold stressed with coats on (coated wet controls) and in the controls not subjected to wetting (dry controls). Plasma cortisol increased markedly in the cold stressed sheep until plastic coats were applied, but the levels had fallen to normal levels 20 h later. Serum thyroxine levels increased at a faster rate in the test group and in wet controls than in the dry controls and coated wet controls during the first 9 h of cold stress. In 4 sheep of the test group energy utilisation increased markedly and remained high for a variable time after coats were applied then gradually returned to pre-cold stress levels. Liver tyrosine aminotransferase levels of the wet controls were markedly elevated compared to levels found in the test group sheep killed 79.5 h after coats were applied. Histological changes in the spleen and liver of wet controls was absent or only mildly present in the test group sheep. There was severe depletion of muscle and liver glycogen in the wet controls compared with the test group sheep, which were similar to the dry controls and coated wet controls.  相似文献   

17.
灰色关联度分析法对不同处理下草坪质量的综合评判   总被引:10,自引:1,他引:10  
应用灰色系统理论对不同施氮量和施肥次数处理下的草坪质量进行了分析与评判.结果表明:施用氮肥是提高草坪质量的重要措施,在35 g/m2施量水平下,草坪质量与施肥量呈正相关(R2>0.9100);施肥次数对其影响不显著.建议在实践中施肥量以25 g/m2为宜.  相似文献   

18.
旨在研制首批泰万菌素兽用国家标准品,用于酒石酸泰万菌素及其制剂中泰万菌素的效价测定。采用精制后的泰万菌素酒石酸盐为原料进行本批泰万菌素标准品的研制,原料分装后按照酒石酸泰万菌素质量标准及《中华人民共和国兽药典》2015年版中的规定对其进行质量检测及均匀性评价,采用紫外-可见分光光度法测定最大吸收波长,60℃减压干燥法测定干燥失重,高效液相色谱法测定泰万菌素A组分,并以原始发明厂的标准品为基准,采用抗生素微生物检定法中三剂量管碟法进行协作标定,对标定结果进行合并计算,作为本批标准品的效价赋值。结果显示,分装后的标准品经60℃减压干燥至恒重后减失重量为2.0%,均匀性评价符合要求,在289 nm的波长处有最大吸收,抗生素微生物检定法测定的泰万菌素效价为822 U·mg-1。本研究研制的首批泰万菌素兽用国家标准品各项检测指标均符合规定,可作为国家标准品发放,用于酒石酸泰万菌素原料及制剂中泰万菌素的效价测定,便于生产企业和质量检定部门进行酒石酸泰万菌素原料及制剂的质量控制,对于提高和保证兽药质量具有重要意义。  相似文献   

19.
A clinical study was performed in 21 dogs to evaluate the efficacy of selamectin for the treatment of naturally acquired infection of sucking lice (Linognathus setosus [L.setosus]) in dogs. Each dog was randomly assigned to one of two treatment groups. One group was treated with selamectin applied topically at a mean dosage of 7.9 mg/kg. The other group was treated with permethrin applied topically at a mean dosage of 85.7 mg/kg. At day 42 posttreatment, all animals remaining in the study (10 treated with selamectin and six with permethrin) were clear of lice. In both groups, the reduction in lice counts from pretreatment values to day 42 was statistically significant at P< or =0.0001. Selamectin applied topically appeared to be effective against L. setosus infection in dogs.  相似文献   

20.
Cattle are normally stunned electrically by three sequential cycles, first a three-second head-only cycle, to stun the animal, secondly a 15-second cardiac cycle, to induce ventricular fibrillation (cardiac arrest), and thirdly a four-second discharge cycle, to reduce convulsions after death. An effective and immediate stun was produced when > or =1.15 amps sinusoidal AC at 50 Hz was applied between the nose and neck electrodes for less than one second. However, when applied for three seconds, head-only currents of >0.46 amp sinusoidal AC at 50 Hz were sufficient to induce epileptiform activity in the brain, identified as high amplitude low frequency activity in the electroencephalogram. The induction of effective head-only electrical stunning resulted in an average interval of 50 seconds before the return of rhythmic breathing movements, and positive corneal and palpebral reflexes. The cardiac arrest cycle successfully induced ventricular fibrillation when >1.51 amps sinusoidal AC at 50 Hz was applied for five seconds between the nose and brisket electrodes.  相似文献   

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