酶解法评定反刍动物饲料干物质和蛋白质降解率的研究

本实验探讨用酶解法测定反刍动物饲料干物质和蛋白质降解率。结果表明,酶解法具有易标准化、重复性高、操作方便和不用实验动物等优点,本实验3次重复,干物质和蛋白质降解率平均变异系数为0.43％和0.87％。通过对一系列培养条件的实验研究,测定出纤维素酶和胃蛋白酶的培养适宜条件。同时用酶解法和尼龙袋法测定了6种饲料的干物质和蛋白质降解率,统计表明两种方法测定值相关显著 (r干物质=0.89,r蛋白质=0.82),并由此而求出用酶解法来估测尼龙袋法测定降解率的回归公式: 干物质降解率:Y=1.31X-16.93 蛋白质降解率:Y=0.92X-0.34     

体外持续发酵法评定反刍动物饲料干物质和蛋白质降解率的研究

研究成功了新持续发酵人工瘤胃装置和方法。研究了该法评定饲料干物质和蛋白质降解率的影响因素及稳定性;测定了6种饲料的干物质和蛋白质降解率并与瘤胃尼龙袋法进行了比较。结果表明该法的稳定性良好,发酵罐内瘤胃液和缓冲液各400ml,缓冲液对发酵罐内容物的稀释率0.0221/小时为宜。用持续发酵法测定的饲料干物质和蛋白质降解率估测瘤胃尼龙袋法降解率的回归式如下: 干物质 Y=-9.38+1.11x r=0.9557,n=6 蛋白质 Y=-1.40+1.01x r=0.9940,n=6     

应用纤维素酶评定秸秆饲料的消化率及其影响因素的研究

应用纤维素酶+胃蛋白酶法评定了6种秸秆饲料在绵羊体内的消化率。试验结果表明:酶解的消化率(X)与体内干物质消化率(DMD),有机物消化率(OMD),和能量消化率(ED)之间有显著的相关性,求出估测方程为: 体内:DMD(％)=6.95+0.75X r=0.89(P<0.01),RSD=1.81 OMD(％)=9.39+0.80X r=0.76(P<0.05),RSD=3.25 ED(％)=0.92X-1.75 r=0.78(P<0.05).RSD=3.54 酶解法具有良好的重演性和稳定性(变异系数平均4.66％)。     

胃蛋白酶法评定反刍动物饲料蛋白质降解率的研究

实验研究了用单一胃蛋白酶法评定反刍动物饲料蛋白质降解率及其主要因素,并研究了三种不同分离方法的稳定性,建立了体外胃蛋白酶法评定饲料蛋白质降解率的方法,用该法研究了六种饲料蛋白质降解的动态规律。同时对酶解法测得的饲料蛋白质降解率进行瘤胃动态消化模型处理,并将处理结果以及酶解法不同时间点蛋白质降解率分别与体内法和尼龙袋法进行比较。     

两步酶解法评定豆粕粗蛋白质降解率的研究

虽然尼龙袋法是国际公认的评定饲料蛋白质瘤胃降解率的常规方法,但是尼龙袋法耗时、复杂,受瘘管动物的限制,不能满足大规模评定饲料蛋白质降解率的要求。为了寻求能在一般实验室条件下即可完成的体外评定饲料蛋白质降解率的方法,国内外做了大量的研究,提出了体外发酵法、动物模型法、酶解法等典型的体外法。其中以酶解法的操作最为简便,费用最低,结果也与体内法和尼龙袋法具有较强的相关性。试验的目的是研究用胃蛋白酶—胰蛋白酶两步酶解法评定保护处理豆粕中粗蛋白质降解率的最适反应条件,包括最适酶浓度、底物浓度、缓冲液pH值、水浴温…     

非淀粉多糖酶对蛋鸡玉米——杂粕型日粮离体消化能的影响

选用木聚糖酶(x1)、β-葡聚糖酶(x2)、纤维素酶(x3)和果胶酶(x4)4种非淀粉多糖(NSP)酶制剂,利用胃蛋白酶-胰液素法分别研究其对蛋鸡玉米—杂粕型日粮能量的当量调控。结果表明,4种NSP酶对蛋鸡玉米—杂粕型日粮的离体消化能改进值分别为:木聚糖酶0.5442MJ/kg、β-葡聚糖酶0.4791MJ/kg、纤维素酶0.3345MJ/kg、果胶酶0.4457MJ/kg。4种NSP酶的添加水平与离体消化能(y)进行S曲线拟合,其回归方程分别为:y1=10.6219-0.6354/｛1+exp([x1-446.6123)/146.000](｝R2=0.9961);y2=10.5065-0.5687/｛1+exp[(x2-282.9857)/146.599](｝R2=0.9964);y3=10.3612-0.3464/｛1+exp[(x3-468.4880)/93.931]｝(R2=0.9939);y4=10.4684-0.4669/｛1+exp[(x4-367.4321)/100.142]｝(R2=0.9989)。     

酶解法评定青粗饲料有机物降解率的研究

用胃蛋白酶-纤维素酶的复合酶解法测定苜蓿干草、红三叶草、豆腐渣、啤酒糟、高梁青贮、羊草、雀麦草、羊茅和稻草的有机物降解率(FOM,％)分别为51.16、70.88、82.29、46.92、44.32、29.64、44.88、45.92、30.92。盐酸胃蛋白酶-纤维素酶技术能很好地估测青粗饲料在瘤胃中的降解率(FOM),对于牧草类FOM值的估测价值为R~2=92％以上(r>0.96)。其操作程序为:(1)0.1N HCI-胃蛋白酶(酶量为150IU/0.3g粗料)在40℃下培养24小时;(2)在pH=4.5的柠檬酸盐缓冲液中用纤维素酶(50mg酶量)培养24小时(40℃)。该法评定青粗饲料FOM不必加水解淀粉的步骤。     

回直肠吻合猪十二指肠活动尼龙袋测定饲料回肠消化率初探

用4头大约克×长沙大围子杂交阉公猪做回直肠吻合术 ,其中两头装有十二指肠“T”型瘘管。分别用全收糜法(CCD)和可动尼龙袋法(NMBT)测定猪饲料营养物质回肠消化率。结果表明 :(1)活动尼龙袋法测得的粗蛋白消化率显著(P<0.05)或极显著(P<0.01)地高于全收糜法表观消化率 ,与真消化率相近 ;干物质消化率则与全收糜法的表观消化率相近。(2)从消化道排出的尼龙袋经过漂洗后 ,消化率比未经漂洗的尼龙袋高(P<0.05) ,漂洗4分钟与8分钟的养分消化率差异不显著(P>0.05)。胃蛋白酶浓度在0.03 %～0.10 %的范围内对消化率没有影响。(3)尼龙袋样品经酸性胃蛋白酶溶液预处理4小时与2.5小时 ,对大多饲料的养分消化率没有显著影响(P>0.05);只有鱼粉预处理4小时的消化率高于预处理2.5小时(P<0.01)。(4)回直肠吻合猪十二指肠活动尼龙袋法测定饲料营养物质回肠消化率是一种快速经济的方法。     

羊常用饲料原料瘤胃有效降解率与各时间点降解率相关关系的研究

为简化饲料瘤胃有效降解率的测定过程,本试验采用尼龙袋法测定了25种羊常用饲料原料(15种精饲料、10种粗饲料)干物质(DM)和粗蛋白质(CP)的瘤胃有效降解率和各时间点降解率,通过分析各时间点降解率和有效降解率之间的相对误差,筛选出可以直接代替有效降解率的单一时间点降解率,并拟合该时间点降解率与有效降解率之间的回归方程。结果表明:1)25种饲料原料DM和CP的16 h降解率与有效降解率之间的平均相对误差最小,分别为9.54%、8.61%;2)25种饲料原料的DM有效降解率(Y)与其16 h降解率(X)呈显著线性相关(Y=0.793 1X+10.767,R2=0.682 1,P0.01,n=25);25种饲料原料的CP有效降解率(Y)与其16 h降解率(X)呈显著线性相关(Y=0.761 8X+13.535,R2=0.821 0,P0.01,n=25)。因此,16 h的瘤胃预培养可以反映饲料到达小肠前的瘤胃降解情况,饲料原料的16 h瘤胃降解率和线性回归方程均可以作为简化饲料瘤胃有效降解率的测定方法。     

刺参肠道蛋白酶、淀粉酶、脂肪酶与纤维素酶活性的测定方法

为探求更有效、更便捷地测定刺参肠道蛋白酶、淀粉酶、脂肪酶与纤维素酶的活性方法,以刺参肠道组织10倍重量的磷酸缓冲液通过匀浆获得粗酶液。采用福林-酚法测定刺参肠道内蛋白酶活力,使用3 ml粗酶液,酪氨酸的回归方程为y=0.000 4+15.028 9x,决定系数R2=0.995 5;采用淀粉-碘法测定淀粉酶活力,使用粗酶液0.3 ml;采用聚乙烯醇橄榄油乳化液水解法测定刺参脂肪酶活性,使用粗酶液5 ml;采用3,5-二硝基水杨酸法测定刺参纤维素酶活力,葡萄糖标准曲线方程为y=0.005 8+0.059x,决定系数R2=0.998 0,使用粗酶液2 ml。由于刺参所摄食的饲料组成不同,其饲料中的组成成分对这4种消化酶活性的影响程度就有很大差异。     

不同加工工艺对玉米纤维饲料营养价值的影响

本试验以干玉米纤维饲料(DCGF)和湿玉米纤维饲料(WCGF)为原料,分析加工工艺对玉米纤维饲料营养价值的影响。选取3头装有永久性瘤胃瘘管的干奶期荷斯坦奶牛为试验动物,采用常规方法测定DCGF和WCGF营养成分含量,采用尼龙袋法测定瘤胃降解特性,采用三步体外法测定瘤胃非降解蛋白质(RUP)的小肠消化率,采用NRC模型预测代谢蛋白质和必需氨基酸供给量。结果表明:1)DCGF常规营养成分中除中性洗涤纤维(NDF)、中性洗涤不溶粗蛋白质(NDICP)、酸性洗涤不溶粗蛋白质(ADICP)含量显著高于WCGF(P0.05)外,其他营养成分含量均差异不显著(P0.05);2)DCGF和WCGF的瘤胃NDF降解率差异不显著(P0.05),而DCGF的瘤胃干物质(4、24、48 h)、粗蛋白质(CP)降解率(12、24、48和72 h)及有效降解率显著低于WCGF(P0.05);3)DCGF的RUP含量显著高于WCGF(P0.05),而总可消化养分和微生物蛋白质、代谢蛋白质含量与DCGF差异不显著(P0.05);4)DCGF的RUP的小肠消化率显著低于WCGF(P0.05),但总可消化蛋白质含量差异不显著(P0.05);5)饲料RUP提供的必需氨基酸中,DCGF的RUP提供的组氨酸(His)、苯丙氨酸(Phe)、异亮氨酸(Ile)以及总必需氨基酸含量显著高于WCGF(P0.05)。综合得出,DCGF和WCGF都含有高含量的可利用纤维和蛋白质,可以作为奶牛良好的纤维和蛋白质源饲料,但DCGF的瘤胃CP降解率和RUP的小肠消化率较低,可能影响其蛋白质和氨基酸的营养价值。     

Nutrient digestibility and energy value of sheep rations differing in protein level, main protein source and non-forage fibre source

Two in vivo digestion trials were conducted to evaluate the effects of diet's crude protein (CP) level, N degradability, and non-forage fibre source (NFFS) on nutrient digestibility and energy value of sheep rations. In each trial, rams were fed four isocaloric and isofibrous rations, differing in main protein and/or NFFS source. At the first trial, mean CP/metabolizable energy (ME) ratio of the diets was 17 g/MJ ME and at the second trial, 13 g/MJ ME. At both trials, the first ration contained cotton seed cake (CSC) and wheat bran (WB), the second CSC and corn gluten feed (CGF), the third corn gluten meal (CGM) and WB and the fourth CGM and CGF. Data of both trials were analysed in common as 2 x 2 x 2 factorial experimental design. Low N degradability (CGM) had positive effect on CP, neutral detergent fibre (NDF) and acid detergent fibre (ADF) digestibility of the ration. Those results suggest that an increase in rumen undegradable protein (RUP) content does not negatively affect nutrient digestibility of sheep rations. Corn gluten feed significantly elevated crude fibre (CF) digestibility, in comparison with WB. Rations having high CP/ME ratio had higher digestibility of CP in comparison with those having low CP/ME ratio; the opposite was true for ether extract, CF, NDF and ADF digestibilities. CP level x N degradability interaction negatively affected energy value of the rations that had high CP level and high N degradability. Former suggest that when CP content is high then N degradability should be low otherwise ration's ME is negatively affected. CP digestibility and coefficient q of the rations containing WB and having high N degradability (N degradability x NFFS interaction) were the lowest suggesting that the combination of CSC and WB negatively affected CP digestibility and energy value of the ration. This could be explained by a reduced microbial CP synthesis, or lower RUP digestibility or both.     

6种饲料原料瘤胃降解特性和瘤胃非降解蛋白质的小肠消化率

本试验旨在研究新疆地区玉米青贮、棉籽壳、苜蓿草粉、苜蓿干草、葡萄籽粕、蕃茄酱渣6种奶牛饲料原料的瘤胃降解特性和瘤胃非降解蛋白质(RUP)的小肠消化率(Idg)。选用3头装有永久性瘤胃瘘管的泌乳荷斯坦奶牛,采用尼龙袋法和改进三步体外法测定饲料原料的干物质(DM)、粗蛋白质(CP)、中性洗涤纤维(NDF)和酸性洗涤纤维(ADF)的瘤胃降解特性以及RUP的Idg和小肠可消化粗蛋白质(IDCP)含量。结果表明:1)苜蓿草粉和玉米青贮的DM有效降解率最高,显著高于次之的苜蓿干草、番茄酱渣(P0.05),葡萄籽粕、棉籽壳最低,显著低于其他原料(P0.05);CP有效降解率为番茄酱渣苜蓿草粉玉米青贮苜蓿干草棉籽壳葡萄籽粕,各原料间差异显著(P0.05);NDF有效降解率为玉米青贮苜蓿草粉棉籽壳苜蓿干草番茄酱渣葡萄籽粕,各组饲料原料间差异显著(P0.05);ADF有效降解率为玉米青贮棉籽壳苜蓿干草番茄酱渣苜蓿草粉葡萄籽粕,各组饲料原料间差异显著(P0.05)。2)苜蓿草粉RUP的Idg和IDCP含量最高,Idg显著高于依次降低的苜蓿干草、玉米青贮、番茄酱渣、葡萄籽粕、棉籽壳(P0.05),IDCP含量与苜蓿干草差异不显著(P0.05),显著高于依次降低的玉米青贮、番茄酱渣、葡萄籽粕、棉籽壳(P0.05)。综上所述,不同饲料原料具有不同的瘤胃降解特性,进入小肠IDCP的含量也不同。玉米青贮的DM、NDF和ADF在瘤胃的有效降解率较高,苜蓿草粉RUP的Idg较高,苜蓿草粉和苜蓿干草的IDCP含量较高。     

Effect of harvest date and variety on ruminal degradability of ensiled maize grains in dairy cows.

In this study we investigated the influence of harvest date and genotype on the ruminal degradability of the organic matter of ensiled maize grains. Grains of the varieties Avenir, Byzance, CGS 5104 and CGS 5107 from six different harvest dates were available; they are classified as intermediate types between flint and dent corn. The six harvest dates, during which time the dry matter content of the ensiled grains rose from 52% to 66%, extended from 1st September to 19th October. Assuming a passage rate of k = 0.08, the effective ruminal degradability declined in this period on average from 93% to just under 79%; variety-specific deviations also increased markedly during this period. The dry matter content (x, DM in %) of the ensiled grains had a profound influence on ruminal degradation: a highly significant curvilinear decline in ruminal degradability (y) was calculated at increasing DM levels (k = 0.08), which can be described by the equation y = -0.072x2 (+/- 0.010) + 7.417x (+/- 1.186) - 98.71 (+/- 34.58) (B = 0.96; sy.x[%] = 1.36). The ruminal degradability of ensiled maize grains is about 5-10% higher than that of fresh maize grains.     

Nutritive evaluation of legume seeds for ruminant feeding

Chemical composition, rumen degradability and the effect of particle losses, and intestinal digestibility of protein by using in situ‐in vitro and in vitro techniques were stated for beans (Vicia faba), lupin (Lupinus albus), vetch (Vicia sativa) and bitter vetch (Vicia ervilia) and four diets including those legume seeds. In addition, the apparent digestibility of experimental diets was determined in goats. The legume seeds showed high protein content (206–319 g/kg dry matter). Effective degradability of protein for legumes and diets varied from 0.80 to 0.87 and 0.76 to 0.82, respectively, decreasing to 0.53–0.76 and 0.61–0.67, respectively, when particle loss was taken into account. Different intestinal digestibility values were obtained with both methodologies without significant relationship between them (y = 1.058–0.463x; R² = 0.068; RSD = 0.140; p = 0.53). There were no differences in the apparent nutrients and energy digestibility among diets (p > 0.05). These legumes can supply rapidly degradable protein for microbial protein synthesis and contribute to the pool of amino acids available for the synthesis of milk protein and for retention in the body.     

Diversity of chemical composition, dry matter intake, in vivo digestibility and in situ dry matter degradability of oat hay (Avena sativa)

Diversity of chemical composition, in vivo digestibility and in situ dry matter degradability of oat hay (Avena sativa) were studied. Oat hay samples obtained from 22 batches imported were analyzed for chemical composition. The result showed a wide range of variations in crude protein (3.9–8.8%, on a dry matter basis), crude fiber (22.1–35.2%), nitrogen free extract (50.0–66.0%), organic cellular contents (23.4–40.0%), organic cell wall (53.1–70.8%), organic a (4.1–9.8%), organic b (49.0–63.8%) fractions and the estimated total digestible nutrients (50.3–63.8%) from the organic components. Of these, four oat hay samples were used for the measurement of dry matter intake, in vivo digestibility and in situ dry matter degradability in sheep. Significant differences were observed for the dry matter intake, in vivo digestibility of dry matter, crude protein, crude fiber and nitrogen free extract, dry matter disappearance at 48 h and effective degradability at k = 0.05 h^?1 of the samples (P < 0.05). The results revealed a wide range of diversity of nutritional quality of the imported oat hay that could have an affect on production potential in animals.     

Prediction of digestible energy content of extruded dog food by in vitro analyses

The aim of this study was to develop a simple and reproducible in vitro method for predicting the apparent energy digestibility of dry extruded dog foods. The proposed method is based on the two-step multienzymatic incubation assay described by [Boisen, S., 1991: In Vitro Digestion for Pigs and Poultry, M. F. Fuller (ed.). CAB International, Wallingford, 135-146], with some modifications adapted to dogs' digestion characteristics. The method consisted in two consecutives incubations, first one during 2 h with pepsin (10 mg/g of food sample) in acid pH and second one during 4 h with pancreatin (100 mg/g of food sample). The undigested residue obtained was collected in a filtration unit and then dried and ashed. The in vitro percentage of organic matter disappearance (in vitro dOM) of 54 dry extruded commercial dog foods was determined and used as predictor of the in vivo apparent organic matter (in vivo dOM) and energy digestibility (in vivo dE) and digestible energy (DE) content. There was a close linear relationship between the in vivo dOM and dE [r(2) = 0.95, residual standard deviation (RSD) = 1.05 and coefficient of variation (CV) = 1.2%] and also between the in vitro and in vivo dOM (r(2) = 0.92, RSD = 1.38 and CV = 1.6%), even if the in vitro dOM overestimated on average by 4% the in vivo dOM. When the in vitro dOM was used to predict the in vivo dE, the relationship between both variables was defined by the equation: in vivo dE (%) = -2.45 + 0.98 +/- 0.04x in vitro dOM (%), (r(2) = 0.92, RSD = 1.25 and CV = 1.5%). In addition, a close relationship between the in vivo and predicted DE (estimated dE x gross energy determined calorimetrically) was found (r(2) = 0.97, RSD = 0.26 and CV = 1.4%). The accuracy of DE content prediction using the proposed in vitro method was higher than that obtained when the DE content of the same set of samples was predicted by the equation proposed by the NRC (1985) (r(2) = 0.76, CV = 4.5%) and also slightly higher than that obtained when using the equation proposed by NRC (2006) (r(2) = 0.95, CV = 2.1%).     

葡萄糖处理豆粕对蛋白质保护效果的研究

以3头装有永久性瘤胃瘘管的中国荷斯坦奶牛为试验动物,采用尼龙袋法研究分别添加0、1%、3%、5%、7%、9%葡萄糖保护豆粕,对奶牛瘤胃内不同时间点DM、CP消失率及其动态降解率的作用效果。结果表明:在不同时间点下,提高葡萄糖添加量可使DM、CP消失率逐渐降低,所有试验组与未处理组差异均极显著(P<0.01),DM、CP有效降解率降低最高分别达28.78%和24.82%,不同添加量下,添加量为7%、9%的2组组间差异不显著(P>0.05)。本试验结果证明,葡萄糖加热处理是一种有效保护豆粕蛋白质的方法,且葡萄糖的适宜添加水平为7%。     

反刍家畜常用饲料蛋白质和氨基酸瘤胃降解特性和小肠消化率评定研究

本试验探讨了常用饲料在瘤胃的降解特性及非降解饲料的小肠消化率,旨在为研究反刍动物的营养平衡和消化规律及科学配制日粮提供依据。试验选用3头装瘤胃瘘管和十二指肠瘘管的肉牛,采用尼龙袋法研究反刍动物常用饲料粗蛋白质和氨基酸瘤胃降解参数和表观小肠消化率。结果表明,在本试验中粗蛋白质降解率由低到高的顺序为:酒糟蛋白、黄玉米、羊草、玉米胚芽饼、菜粕、棉粕、豆粕、花生饼、啤酒糟、苜蓿、米糠、小麦麸;总氨基酸瘤胃降解率由低至高的顺序依次为:酒糟蛋白、黄玉米、羊草、玉米胚芽饼、菜粕、豆粕、棉粕、啤酒糟、花生饼、苜蓿、米糠、小麦麸。除花生饼、酒糟蛋白和黄玉米外,粗蛋白质和总氨基酸的有效降解率差异不显著(P>0.05)。粗蛋白质的表观小肠消化率由低至高的顺序依次为:苜蓿、羊草、米糠、小麦麸、啤酒糟、玉米胚芽饼、棉粕、菜粕、玉米、酒糟蛋白、花生饼、豆粕;总氨基酸的小肠消化率由低至高的顺序依次为:羊草、苜蓿、米糠、小麦麸、啤酒糟、玉米胚芽饼、菜粕、棉粕、花生饼、玉米、酒糟蛋白、豆粕。由此可见,不同的饲料瘤胃降解特性是不同的,并且为小肠提供的各种可吸收氨基酸潜力也是不同的。     

基于康奈尔净碳水化合物与蛋白质体系的瘤胃非降解蛋白质小肠可吸收氨基酸流量的简化评定技术

本试验旨在简化基于康奈尔净碳水化合物与蛋白质体系(CNCPS)评定瘤胃非降解蛋白质(RUP)小肠可吸收氨基酸流量的技术。小肠可吸收氨基酸来自菌体蛋白和RUP,CNCPS根据溶解性将饲料粗蛋白质(CP)分为A、B1、B2、B3和C共5种组分,只有3种B组分可以过瘤胃并在小肠中消化。为评定RUP小肠可吸收氨基酸的贡献,CNCPS需要分别测定3种B组分的瘤胃降解率,3种过瘤胃B组分的小肠消化率需采用不同常数。选择18份饲料样品,其中精料12份,粗料6份,测定CNCPS评定RUP小肠可吸收氨基酸流量所需数据,同时对饲料CP的瘤胃动态降解率及不同时间点RUP的体外小肠消化率进行了测定,通过这些数据提出简化评定方法。结果表明:1)精料和粗料均以8 h的CP瘤胃降解率(X,%)与有效降解率(Y,%)间相关性最强,二者间呈线性相关,精料和粗料方程分别为Y=12.652+0.828X,r=0.990,P0.000 1和Y=10.967+0.886X,r=0.980,P=0.000 6。2)精料2 h RUP小肠消化率(X,%)与RUP小肠有效消化率(Y,%)间相关性最强,方程为Y=0.026+0.879X,r=0.970,P0.000 1;粗料8h RUP小肠消化率(X,%)与RUP小肠有效消化率(Y,%)间相关性最强,方程为Y=-0.002+0.960 X,r=0.995,P0.000 1。3)简化方案经可靠性评估得出,8 h CP瘤胃降解率和RUP小肠消化率简化CNCPS模型预测的小肠氨基酸流量(X,‰)与CNCPS预测的小肠氨基酸流量(Y,‰)相关性最强,精料方程为Y=-0.056+1.409X,r=0.999,P0.000 1;粗料方程为Y=0.003+2.120 X,r=0.999,P0.000 1。精料和粗料的简化评定结果与CNCPS评定结果的均方根误差分别为0.245和0.005,变异系数分别为7.08%和4.49%。综合得出,基于CNCPS,得到了预测RUP小肠可吸收氨基酸流量的简化模型,简化后的精料和粗料模型分别为Y=-0.056+1.409×[AA×(100-D8)×CP×ID8],r=0.999,P0.000 1和Y=0.002+2.120×[AA×(100-D8)×CP×ID8],r=0.999,P0.000 1,Y为RUP中的某种氨基酸小肠可吸收流量(‰),D8为CP的8 h瘤胃降解率(%),ID8为RUP的8 h小肠消化率(%),AA为不溶性蛋白质中该氨基酸含量(%)。