浅谈猪圆环病毒2型（PCV-2）

PCV-2是近几年来一种新发现的病毒,它所致疾病能给养猪业带来很大的经济损失。 PCV为圆环病毒科圆环病毒属成员,单股环状DNA病毒。PCV存在两种血清型,即PCV-1(猪圆环病毒1型)和PCV-2(猪圆环病毒2型)。PCV-1经常存在于机体各组织器官和某些传代细胞中,不致病。PCV-2则是引起PMWS(猪断奶后多系统衰竭综合征)的主要病原。据发现,单     

猪圆环病毒2型的危害及综合防制

猪圆环病毒病是由猪圆环病毒2型（porcine circovirus 2, PCV2）引起猪的一种传染病,是近几年来发现的较难控制的传染病。猪圆环病毒属于圆环病毒科、圆环病毒属的成员,是目前发现的最小的动物病毒。根据圆环病毒的致病性、抗原性及核苷酸序列可分为PCV1和PCV2两个型,     

甘肃省断奶仔猪圆环病毒病的调查

猪圆环病毒病是新近传入我国的猪病,由圆环病毒2型(Post-weaning mlutisystemic wasting syndrome,PCV2)引起,郎洪武等(2000年)采用ELISA方法进行了PCV2的检测,     

猪圆环病毒研究进展

猪圆环病毒(Porcine circovirus,PCV)是迄今发现的最小的动物病毒之一.PCV具有PCV1和PCV2两种基因型.PCV1无致病性.PCV2有致病性,它不仅是断奶猪多系统衰竭综合症(Post -weaning multisystemic wasting syndrome,PMWS)的主要病原(Bolin等,2001;McNeilly等,2001),而且是其他诸多传染病的病原之一.世界各国的兽医与养猪业者认为它是继猪繁殖与呼吸道综合征之后新发的重要的猪传染病,引起了国内外学者的广泛关注.     

猪圆环病毒Ⅱ型病鉴别诊断技术的研究进展

猪圆环病毒（PCV）属于圆环病毒科,圆环病毒属,是一种无囊膜的二十面体的单链环状DNA病毒,该病毒有两个血清型：猪圆环病毒I型（PCV1）和猪圆环病毒II型（PCV2）。猪圆环病主要是由PCV2引起。本文旨在通过讨论PCV2的临床症状及鉴别诊断方法,为PCV2的现场及实验室鉴定提供理论依据。     

吉林省猪圆环病毒Ⅱ型血清流行病学调查

猪圆环病毒(porcine circovirus,PCV)为圆环病毒科(Circoviridae)圆环病毒属成员,单股环状DNA病毒,是迄今发现的最小的动物病毒。PCV存在两种基因型,即PCV-1和PCV-2。PCV-1未发现有致病性;PCV-2主要引起仔猪免疫缺陷的断奶仔猪多系统消耗综合症(Post-weaning multisystemic wasting syndrome,PMWS)的病原之一。猪是     

与猪圆环病毒Ⅱ型感染相关几种疾病的诊断

目前猪圆环病毒病已被世界各国公认为是继猪繁殖与呼吸综合征(PRRS)之后发现的引起猪免疫抑制的重要传染病,是由猪圆环病毒(PCV)引起的传染病。现已知有2个血清型,即PCV1和PCV2,PCV1为非致病性的病毒;PCV2(圆环病毒Ⅱ型)为致病性的病毒,该病毒会严重侵害猪的免疫系统,导     

保定地区猪圆环病毒2型ORF2基因的克隆与序列分析

猪圆环病毒(porcine circovirus,PCV)是为无囊膜单股环状负链DNA病毒,病毒粒子直径约17～20 nm,是迄今已知最小的动物病毒之一.PCV属于圆环病毒科圆环病毒属成员,根据其致病性及抗原性将它分为两个血清型,即PCV1和PCV2.     

猪圆环病毒2型对猪的免疫抑制机理

<正>猪圆环病毒2型(PCV2)是圆环病毒科(Circoviridae)圆环病毒属的一员,分PCV1型和PCV2型,PCV1型不致病,PCV2型致病。猪圆环病毒2型(Porcine circovirus type2,PCV2)     

辽宁省某规模化猪场猪圆环病毒Ⅱ型的PCR检测

猪圆环病毒(Porcine circovirus,PCV)是迄今为止发现的最小的一种脊椎动物病毒。PCV分为无致病性(或PK15源性)的猪圆环病毒Ⅰ型(PCV-1)和有致病性(PMWS源性)的猪圆环病毒Ⅱ型(PCV-2)两个基因型[1-2]。PCV-1广泛存在于猪体内及猪原代细胞系中,无致病性,不能引起细胞病变;     

癸氧喹酯干混悬剂含量测定的改进

采用高效液相色谱法测定癸氧喹酯干混悬剂的含量,在2-250μg/mL范围内,峰面积的常用对数与进样量浓度的常用对数呈良好的线性关系,R^2=1(n=5),平均回收率为99.24%~99.51%,RSD在0.05%~0.28%。此方法分析时间短,样品前处理简便、定量结果准确,重现性好,结果满意,为其质量控制提供了依据。     

猪毛色遗传的研究进展

本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。     

商会自治的基石——商会自治规范研究

在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。     

Effects of size of ingestively masticated fragments of plant tissues on kinetics of digestion of NDF

Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo.     

中华人民共和国农业部公告 第267号

为贯彻落实《兽药生产质量管理规范》(简称《兽药GMP》),进一步推动兽药GMP实施进程,我部制定了《兽药生产质量管理规范检查验收办法》,现予公告。本公告自2003年6月1日起施行。附件:兽药生产质量管理规范检查验收办法二○○三年四月十日第一章 总则 第一条 为推动《兽药生产质量管理规范》(以下简称兽药GMP)的实施,规范兽药GMP检查验收工作,制定本办法。 第二条 农业部负责全国兽药GMP管理和检查验收工作;负责制修订兽药GMP检查验收管理规定;负责兽药GMP检查员队伍建设和监督管理工作,负责国际兽药贸易中GMP互认工作。 …     

鸡败血支原体垂直传播模式及其阻断方法

以国际标准强毒Ｒ株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离ＭＧ,并收集感染鸡所产蛋分离ＭＧ。结果表明,人工感染４８小时后上、下呼吸道及肺已被全面感染,９６小时气囊已被感染,１２０小时输卵管已能分离到ＭＧ,卵巢始终分离不到ＭＧ。人工感染鸡自１４４小时便能在其所产蛋中分离出ＭＧ。药物治疗能在７２小时内消除感染,油乳剂苗则需２４天后逐渐降低蛋内ＭＧ分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内ＭＧ,但以研制的种蛋浸泡剂药浴效果为最好。     

Mechanism of Action of Probiotic Function of Lactobacilli

乳酸杆菌作为益生菌广泛用于人和动物.本文综述了乳酸杆菌改善宿主健康的机制.乳酸杆菌可通过产生抗菌物质如乳酸、过氧化氢、细菌素,或者通过竞争营养或肠道黏附位点来抑制致病菌;通过诱导黏附素的分泌或阻止细胞凋亡而增强肠道的屏障功能,从而保护肠道.文章重点讨论了乳酸杆菌表面成分(表面蛋白、脂磷壁酸和肽聚糖)与肠道受体(C型凝集素受体、Toll样受体和Nod样受体),阐述了他们结合后启动免疫调节信号,调控肠道免疫功能以发挥改善健康作用的机制.     

獭兔睾丸的组织学观察

家兔作为一种实验动物 ,推动了繁殖技术的发展。本试验通过对不同年龄公獭兔的睾丸进行组织学观察、测定 ,研究精子的发生规律 ,为系统地进行繁殖生理工作提供依据。1　材料与方法选 60日龄、75日龄、90日龄 3个年龄公獭兔各5只 ,用外科手术法摘取两侧睾丸 ,放入 Bouin氏液中固定 ,二甲苯透明 ,石蜡包埋 ,切成 5～ 8μm切片 ,H.E.染色。在显微镜下观察 ,并进行定量组织学指标测定及差异性比较。2　结果和讨论2 .1　睾丸定量组织学指标的测定结果　见表 1。表 1　獭兔睾丸定量组织学指标　　　μm,个 /精细管60日龄 75日龄 90日龄曲细精管…     

Observations of fracture of the anconeal process of the ulna of swine

Fractures of the anconeal process of 5 pigs ranging in age from 4 to 8 months were studied radiographically and histologically. Clinically, animals with a fracture of the anconeal process had a "tight," restricted gait. In pigs at 4.5 months of age, a radiolucent line through the base of the anconeal process was composed of fibrocartilage, fibrous connective tissue, and hyaline cartilage. Subperiosteal proliferation of woven bone was located along the cranial surface of the olecranon, adjacent to the base of the anconeal process. In older animals, the radiolucent line through the anconeal process contained variable amounts of fibrous connective tissue and fibrocartilage. The proliferation of subperiosteal bone at the base of the anconeal process formed a "buttress callus" which retained a radiolucent area between the callus and the proximal surface of the anconeal process. The latter region of radiolucency was continuous with the transversely oriented line that traversed the base of the anconeal process.