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1.
为了解宁夏地区猪群中萨佩罗病毒(PSV)的感染流行情况,本研究通过RT-PCR的方法对不同区域的2个规模化养猪场的160份临床健康猪咽拭子和肛拭子样品进行了检测。试验表明,所检的2个养殖场均存在PSV感染,总体平均阳性率为61.25%,其中咽拭子检出率为7.5%,肛拭子猪检出率为58.75%。试验结果表明PSV在宁夏地区普遍存在。  相似文献   

2.
目的为某规模化猪场猪伪狂犬病病毒野毒株感染防控工作提供科学的免疫防控方案。方法对某发病猪群(采用Bartha-K61经典毒株疫苗进行了猪伪狂犬病免疫)进行流行病学调查,并采用ELISA方法检测其猪伪狂犬病gB抗体和gE抗体水平。结果根据调查、解剖和血清抗体检测结果,初步确诊该发病猪群为猪伪狂犬病野毒感染。通过采取紧急免疫伪狂犬病HB2000毒株疫苗、调整免疫程序和配合药物治疗等措施,育肥猪死亡率从2.56%降低至0.60%;除了4周龄及12周龄猪群外,其余猪群伪狂犬病野毒抗体水平全部下降;种公猪、8周龄、10周龄、14周龄猪群伪狂犬病野毒抗体阳性率均为0。结论Bartha-K61经典毒株疫苗并不能提供完全的保护力,通过紧急免疫与流行毒株同源性较高的HB2000毒株疫苗,加强生物安全管理工作,能够有效控制猪伪狂犬病野毒感染。  相似文献   

3.
收集广西境内39个市(县)137个不同规模猪场及农村散养户146份病料,经检测发现广西"猪高热综合征"主要病原以PRRSV变异株(NSP2基因缺失)为主,检出率高达59.59%,其次为PCA占41.78%,CSFV占4041%,SIV占10.27%,PRV、HPS均占2.05%.将来自广西不同地区的15株PRRSV变异株(包括1株经典株)与国内外参考毒株部分Nsp2序列进行同源性比较,结果发现广西PRRSV变异株Nsp2序列之间同源性为83.3%~100%,与国内其他变异株同源性为83.2%~98.6%.这些毒株与国内其他变异株有完全一致的缺失特征.广西PRRSV变异株可分为2个亚群,亚群Ⅰ主要以桂西北地区毒株组成,亚群Ⅱ主要以桂东南地区毒株组成.所有的广西PRRSV流行株与国内其他毒株一样均属于美洲型.调查结果显示,广西"猪高热综合征"主要病原PRRSV变异株与PCV、CSFV等其他病原菌混合感染十分严重.其中,二重感染、三重感染分别高达42.53%和22.99%.  相似文献   

4.
为建立快速检测猪萨佩罗病毒(PSV)的血清学方法,本研究以原核表达的PSV 3C重组蛋白为包被抗原,通过反应条件优化,建立了一种PSV重组3C蛋白的间接ELISA抗体检测方法。结果显示,该ELISA方法仅对PSV血清检测为阳性,与猪瘟病毒、猪繁殖与呼吸综合征病毒、猪伪狂犬病毒、猪圆环病毒2型等主要猪源病毒阳性血清均无特异性反应,具有良好的特异性。该方法的批内和批间重复性变异系数均小于10%,血清稀释度可以达到1∶320;采用该ELISA方法对我国2016年间采集的江苏省6个不同地区猪场的281份临床血清样品进行了检测,阳性率高达38.43%,表明江苏地区猪群中存在PSV感染。本实验建立的ELISA方法可以用于检测临床样品中的PSV抗体,特异性强、敏感性高、重复性好,是PSV流行病学调查的一种有效工具,对该病的防控具有重要意义。  相似文献   

5.
为了解广西壮族自治区是否存在猪细小病毒6型(PPV6)毒株,分析其分子特征和遗传进化,本研究利用PCR技术对广西壮族自治区的猪血清临床样本进行检测。结果表明,广西壮族自治区猪群有PPV6的存在,感染率为18.5%(25/135),与PCV2的共感染率为24.0%(6/25)。与参考毒株的NS1核苷酸同源性为99.4%~99.6%;NS1遗传进化分析表明,GX44毒株和GX3-3毒株与中国参考毒株TJ株、韩国KSU1-AZ-2014株、波兰P15-1株同处一个亚群,而美国U18-9株属于另一个亚群。这一研究结果将为中国PPV6毒株的流行病学调查与分析提供最基础的研究资料。  相似文献   

6.
正猪繁殖与呼吸综合征(PRRS)是一直困扰猪场的一个重大疾病。该病病原--猪繁殖与呼吸综合征病毒--是一种变异较快的病毒,因此临床上存在大量致病力与感染力差异较大的病毒株。致病力与感染性不同毒株在猪群广泛存在,导致猪群不同阶段的猪只长期感染,在猪场形成诸多亚群继而导致循环感染。此外,由于猪蓝耳病病毒具有免疫抑制的负面效应,所以目前疫苗免疫难以彻底解决猪群猪蓝  相似文献   

7.
为研究广西地区是否存在猪细小病毒6型(PPV6)毒株流行,分析PPV6毒株遗传进化和分子特征,采用PCR技术对广西地区采集的猪组织样品进行PPV6基因片段检测。结果表明,广西地区猪群存在PPV6,感染率为36%(27/75),同时也存在着PPV6与猪圆环病毒2型(PCV2)混合感染现象,感染率为32%;5株PPV6 VP1基因与中国参考株TJ株、韩国参考株KSU1-AZ-2014株、美国参考株U18-4株、波兰参考株P15-1株核苷酸序列同源性分别为99.3%~99.8%,99.3%~99.8%,98.9%~99.8%,99.3%~99.8%;VP1基因遗传进化分析表明,PPV6包含两个基因亚群,5株PPV6与中国参考株BJ、SC、JS、TJ株及波兰参考株处在G2亚群,而美国U18-4株则单独处于一个G1亚群。本研究为中国PPV6毒株流行病学及其病毒致病性研究提供研究基础。  相似文献   

8.
对不同种鸡场不同周龄肉种鸡进行REV、CAV和ALV(A、B亚群)抗体检测。在572份血清样品中,除了一个8.1周龄和15周龄鸡群CAV抗体阳性率分别为13.3%和75%外,其它鸡群无论是否进行CAV疫苗免疫,抗体阳性率均为100%。在212份血清样品中,REV抗体阳性率在16.7%~62.5%之间;ALV(A、B亚群)抗体阳性率在0%~75%之间。本研究结果表明,所检测种鸡群中存在3种免疫抑制性病毒的感染或混合感染。  相似文献   

9.
为了解广西猪群猪圆环病毒3型(PCV3)的遗传变异情况,以PCV3阳性DNA为模板,利用PCR的方法分2段扩增PCV3全基因序列并进行拼接,获得10株PCV3全基因组序列,全长均为2 000 bp。10株广西PCV3全基因组序列之间的核苷酸同源性为98.6%~99.8%。与国内其他毒株的同源性为97.9%~99.8%;与国外其他毒株的同源性为98.8%~99.7%。10株广西PCV3 Cap基因的大小均为645 bp,编码214 aa。PCV3 Cap基因比较保守,10株广西PCV3 Cap基因与参考株相比,仅存在散在的突变位点。基于PCV3全基因和Cap基因构建的遗传进化树显示,两者结果基本相同,10株广西PCV3处于3a和3b两个亚群。本试验表明,广西地区猪群中流行的PCV3至少存在2个亚群,不同亚群PCV3毒株的致病性差异还有待进一步的研究。  相似文献   

10.
2018年11月,湖南郴州某规模化猪场发生2~3日龄仔猪呕吐、腹泻为主的疫情,发病率70%,死亡率80%,为确定引起仔猪腹泻的原因,从发病猪群中采集2头病死猪的小肠、肺脏、淋巴结等组织进行PCR检测及基因测序分析。检测结果显示猪流行性腹泻和猪蓝耳病均为阳性,猪传染性胃肠炎、猪轮状病毒均为阴性。测序分析显示所检测到的猪蓝耳病病毒与JXA1毒株高度同源,为高致病性毒株,检测到的猪流行性腹泻病毒为变异毒株,隶属于GⅡ-b群。综合分析,引起此次新生仔猪大批死亡系变异猪流行性腹泻病毒混合感染高致病性猪蓝耳病所致。  相似文献   

11.
In late November 1991, 1883 clinics in Canada were sent a questionnaire to assess the status of Dirofilaria immitis in dogs in 1991 and there was a 60.0% response. There were 344,031 dogs tested for heart-worm (HW), 627 were found infected and the prevalence of HW infection was 0.18%. There were 417 dogs with HW in Ontario, 116 in Manitoba, 38 in Quebec, 53 in British Columbia, three in Alberta, and one in Nova Scotia. In British Columbia, all of the infected dogs but one were from the Okanagan valley which, as from 1991, is a new focus of infection in Canada. Most dogs with HW had not been on preventive medication in 1990, and the prevalence among dogs tested and unprotected was 0.59%. That prevalence was considerably higher in endemic areas. Companion dogs, over three years of age and maintained primarily outdoors in rural areas, were most frequently infected. One cat was diagnosed with D. immitis and 33 dogs had Dipetalonema reconditium.  相似文献   

12.
Heartworm in dogs in Canada in 1983   总被引:6,自引:6,他引:0       下载免费PDF全文
In late December 1983, 2 800 veterinarians across Canada were sent a questionnaire in order to assess the status of heartworm disease in Canada in 1983 and 26% of them responded. Veterinarians reported that 59 504 dogs were blood-tested to check for microfilariae and 771 dogs (1.30% of those tested) were found with Dirofilaria immitis. Heartworm disease was diagnosed in all provinces except New Brunswick and Newfoundland but most (733) of the cases were in Ontario.

Heartworm disease was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 31% of the cases were observed with clinical signs of heartworm disease and 64% had a history of not having left Canada. Southwestern Ontario continues to be the focus of the infection and most of the dogs there had not left the province previously.

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13.
14.
Heartworm in dogs in Canada in 1989   总被引:1,自引:1,他引:0       下载免费PDF全文
In late November 1989, 1732 clinics and institutional veterinarians were sent a questionnaire to assess the status of Dirofilaria immitis, and 51.7% responded. Of 247,716 dogs tested, 394 had D. immitis microfilariae and 51 were amicrofilaremic for a total of 445 cases and heartworm prevalence of 0.17%. Most (408) of these dogs had no preventive medication and the prevalence among dogs tested and unprotected was 1.01%. That prevalence was considerably higher in endemic areas. Thirty-seven dogs with heartworm had preventive medication. Heartworm was most frequent in companion dogs over three years of age maintained outdoors in rural areas. About 75% of the cases had never left Canada, 26% had clinical signs and 125 were not treated.

Heartworm was reported from British Columbia, Manitoba, Ontario, Quebec, Nova Scotia and Newfoundland, but 383 cases were in Ontario. South-western Ontario was the primary focus of infection. There were 33 cases in Quebec and 24 in Manitoba, mainly found in and around Metropolitan Montreal and Winnipeg respectively.

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15.
Heartworm in dogs in Canada in 1987   总被引:1,自引:1,他引:0       下载免费PDF全文
In late November 1987, 1246 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1987, and 50% of them responded. Veterinarians reported that 165,428 dogs were blood tested for heartworm disease and 511 dogs were found with D. immitis microfilariae. Another 78 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1987 as 589 (0.35%).  相似文献   

16.
Heartworm in dogs in Canada in 1986   总被引:2,自引:2,他引:0       下载免费PDF全文
In late December 1986, 1224 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1986; 46% of them responded. Veterinarians reported that 150,989 dogs were blood-tested for microfilariae and 869 dogs were found with heartworm. Another 65 dogs were amicrofilaremic but diagnosed with heartworm disease and one was found with heartworm at necropsy to give the total number diagnosed in 1986 as 935 (0.62%).

Heartworm was reported from Manitoba, New Brunswick, Ontario and Quebec, but most (810) of the cases were from Ontario. South-western Ontario continued to be the primary focus of the infection in Canada. There were 103 cases reported from Quebec, mostly from and around Montreal, and 21 cases from Manitoba, from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 33% of the cases were observed with clinical signs of heartworm disease and 81% had a history of not having left Canada.

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17.
18.
Heartworm in dogs in Canada in 1988   总被引:1,自引:1,他引:0       下载免费PDF全文
In late November 1988, 1581 small and mixed animal clinics and institutional veterinarians across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1988, and 46% of them responded. Veterinarians reported that 181,577 dogs were blood-tested for heartworm disease and 367 dogs were found with D. immitis microfilariae. Another 60 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number of cases diagnosed in 1988 as 441 (0.24%).  相似文献   

19.
Heartworm in dogs in Canada in 1985   总被引:3,自引:3,他引:0       下载免费PDF全文
In late December 1985, 1485 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1985 and 44% of them responded. Veterinarians reported that 137,300 dogs were blood-tested to check for microfilariae and 1210 dogs were found with heartworm. Another 36 dogs were amicrofilaremic but diagnosed with heartworm disease to give the total number diagnosed in 1985 as 1247 (0.91%).

Heartworm was reported from all provinces except Prince Edward Island, Newfoundland and Saskatchewan but most (1126) of the cases were in Ontario. Southwestern Ontario continued to be the primary focus of the infection in Canada. From Quebec, 91 cases were reported mostly from and around Montreal. From Manitoba, 19 cases were reported from Winnipeg and surrounding areas. Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 28% of the cases were observed with clinical signs of heartworm disease and 78% had a history of not having left Canada.

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20.
Heartworm in dogs in Canada in 1984   总被引:4,自引:4,他引:0       下载免费PDF全文
In late December 1984, 1853 institutional veterinarians and small and mixed animal clinics across Canada were sent a questionnaire in order to assess the status of Dirofilaria immitis in Canada in 1984 and 35% of them responded. Veterinarians reported that 97,794 dogs were blood-tested to check for microfilariae and 1417 dogs (1.45% of those tested) were found with heartworm. Another 34 dogs were amicrofilaremic, but were diagnosed as having heartworm disease, to give the total number diagnosed in 1984 as 1451 (1.48%). Heartworm was reported from all provinces except Prince Edward Island and Newfoundland but most (1310) of the cases were in Ontario. In Quebec, 126 cases were reported mostly from west of Montreal.

Heartworm was found most frequently in companion dogs over three years of age maintained mainly outdoors in rural areas. About 27% of the cases were observed with clinical signs of heartworm disease and 72% had a history of not having left Canada. Southwestern Ontario continued to be the primary focus of the infection.

  相似文献   

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