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1例鸡白痢沙门氏菌的分离鉴定与耐药性分析 总被引:1,自引:1,他引:0
为查明河南省新乡市某规模化蛋鸡场疑似沙门氏菌感染病例的病原,进而制定合理的治疗方案,本研究无菌采集疑似病雏鸡肠管样品,用革兰氏染色、培养特性观察、生化试验、PCR方法对其进行确诊,并进行病原回归试验,采用药敏纸片琼脂扩散法(K-B法)分析分离菌对19种常见抗生素的耐药性。结果显示,本试验成功分离了一株革兰氏阴性短杆菌,该分离菌符合鸡白痢沙门氏菌的培养特性和生化特性;PCR成功扩出invA基因,通过与GenBank数据库比对分析确定该细菌为鸡白痢沙门氏菌;用分离细菌株感染SPF鸡能复制出与自然感染一致的病例,说明鸡白痢沙门氏菌是造成本养鸡场雏鸡发病的主要病原;分离株具有较强的致病性和多重耐药性,对阿米卡星、苯唑青霉素、青霉素耐药,对复达欣、氨苄青霉素、头孢曲松等药物敏感,将敏感抗生素用于临床治疗效果明显。结合以上结果,本研究提出该病的具体防治措施,并取得了较好的效果,为鸡白痢沙门氏菌的分离鉴定及防治提供了参考,对鸡白痢沙门氏菌病病原的早期诊断和治疗具有重要临床意义。 相似文献
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为查明河南省新乡市某规模化蛋鸡场疑似沙门氏菌感染病例的病原,进而制定合理的治疗方案,本研究无菌采集疑似病雏鸡肠管样品,用革兰氏染色、培养特性观察、生化试验、PCR方法对其进行确诊,并进行病原回归试验,采用药敏纸片琼脂扩散法(K-B法)分析分离菌对19种常见抗生素的耐药性。结果显示,本试验成功分离了一株革兰氏阴性短杆菌,该分离菌符合鸡白痢沙门氏菌的培养特性和生化特性;PCR成功扩出invA基因,通过与GenBank数据库比对分析确定该细菌为鸡白痢沙门氏菌;用分离细菌株感染SPF鸡能复制出与自然感染一致的病例,说明鸡白痢沙门氏菌是造成本养鸡场雏鸡发病的主要病原;分离株具有较强的致病性和多重耐药性,对阿米卡星、苯唑青霉素、青霉素耐药,对复达欣、氨苄青霉素、头孢曲松等药物敏感,将敏感抗生素用于临床治疗效果明显。结合以上结果,本研究提出该病的具体防治措施,并取得了较好的效果,为鸡白痢沙门氏菌的分离鉴定及防治提供了参考,对鸡白痢沙门氏菌病病原的早期诊断和治疗具有重要临床意义。 相似文献
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运用分子生物学、血清学以及病原分离鉴定等多种方法,对2006~2012年广西规模养殖场、农村散养鸡以及活禽市场鸡血清和组织样本进行鸡白痢-伤寒沙门氏菌抗体和沙门氏菌病原检测和鉴定。结果发现,血清样本中规模场鸡白痢伤寒抗体阳性率较低,平均为2.16%,其中种公鸡的阳性率平均为1.55%,核心群母鸡阳性率平均为1.66%,生产群母鸡阳性率平均为4.16%;但广西农村散养鸡和活禽市场鸡白痢-伤寒沙门氏菌的污染情况不容乐观,其中农村散养鸡白痢-伤寒沙门氏菌阳性率为21.78%~24%,平均为22.52%(320/1421),活禽市场鸡阳性率为20.81%~33.2%,平均达到27.51%(2800/10177);发病鸡的组织样品沙门氏菌平均阳性率为4.9%(29/592),阳性株以伤寒沙门氏菌为主,共13株占44.83%(13/29),其次为亚利桑那沙门氏菌8株,占27.59%(8/29),第三种是沙门氏菌的某些亚种(无法定型),最少为猪霍乱沙门氏菌2株,占6.9%(2/29)。 相似文献
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正禽沙门氏病主要包括禽伤寒、禽副伤寒、鸡白痢3个类型,其中鸡白痢沙门氏菌是危害雏鸡的主要细菌性疾病。当雏鸡感染鸡白痢沙门氏菌后,主要临床症状为排出白色的粪便,严重着可以造成脱水致死,鸡白痢沙门氏菌对雏鸡的感染率和死亡均较高,严重着影响着雏鸡生长发育,并给养鸡户造成严重经济损失。沙门氏菌具有高度宿主适应性,禽伤寒、禽副伤寒、鸡白痢3个不同类型的沙门氏菌只感染禽类引起不同阶段的鸡发病。鸡白痢沙门氏杆菌危害鸡比较严 相似文献
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鸡白痢沙门氏菌的分离与鉴定 总被引:4,自引:0,他引:4
在禽类 ,沙门氏菌可引起鸡白痢、鸡伤寒和副伤寒。其中鸡白痢沙门氏菌感染往往是终生带菌的 ,感染母鸡所产的蛋 ,高达 1/ 3带菌。带菌蛋的孵化率低 ,孵出的雏鸡的成活率也低[1,2 ] 。所以 ,鸡白痢所造成的经济损失是巨大的。2 0 0 2年 8月南宁市某养鸡场有一群 8- 11日龄的雏鸡发病 ,发病率为 10 % ,死亡率为1%。经实验室的细菌分离和一系列的生化试验 ,确诊该群雏鸡感染了鸡白痢沙门氏菌。1 材料与方法1.1 材料1.1.1 培养基血液琼脂平板、麦康凯琼脂平板、伊红美兰平板、半固体培养基等常用培养基 ,由本室制备和保存。1.1.2 生化反应管… 相似文献
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正沙门氏菌是革兰氏阴性菌。鸡群感染后终生带菌,本菌血清型有多种,感染鸡群的主要有鸡白痢沙门氏菌,鸡伤寒沙门氏菌和副伤寒沙门氏菌。鸡白痢沙门氏菌引起雏鸡急性败血症。多侵害15日龄内鸡苗,表现白痢﹑卵黄吸收不良,发病率和死亡率非常高。鸡伤寒沙门氏菌或副伤寒沙门氏菌,引起成年鸡白痢侵害生殖系统 相似文献
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家禽沙门氏菌病是指由沙门氏菌属细菌感染家禽引起的急性或慢性疾病。可分为两大类:一类为不运动的沙门氏菌——鸡白痢和鸡伤寒沙门氏菌感染,曾分别被称为鸡白痢和禽伤寒。最近,已统一使用“鸡白痢-伤寒沙门氏菌”名称来命名这两种沙门氏菌,因此,有专家认为可将这两种疾病统称为禽白痢-伤寒(或鸡白痢-伤寒)。另一类为大量有运动性的沙门氏菌(归类为禽副伤寒沙门氏菌)感染,统称为禽副伤寒。 相似文献
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Extract It is with deep regret that we have to record the death of Mr. Kyle, of Christchruch, who was a widely known and respected member of pur profession. He was born in Melbourne in 1873 and spent the early part of his life in the Riverina district of new South Wales. He graduated as a veterinary surgeon in Melbourne in 1895 and practised in Ballarat until 1901, when he came to New Zealand. He was in government service until 11918 and, after one year in the army, commenced private practice in Christchurch in 1919. He gave up practice on his election to Parliament but resumed on his retirement from office, Mr. Kyle first entered the field of public affairs in local body service and became Mayor of the Borough of Riccarton in 1925, a post which, except for the years 1927 to 1929, he claimed as a record for New Zealand, being 28 years. 相似文献
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Greetje A.A. Castelijn Jo-Ann Parabirsing Marcel H. Zwietering Roy Moezelaar Tjakko Abee 《Veterinary microbiology》2013,161(3-4):305-314
Cross-contamination due to Salmonella on the surface of processing equipment greatly contributes to contamination of pork products. Therefore, a clear understanding of surface and survival behaviour of relevant Salmonella serovars in pork processing environments is needed to develop better strategies for Salmonella control. Within this study the biofilm forming behaviour of S. Typhimurium, S. Derby, S. Brandenburg and S. Infantis isolates was analysed using the crystal violet assay. This assay, commonly used to analyse total biofilm formation, revealed variation in biofilm forming capacity between and within serovars. This has not been shown before for S. Derby, S. Brandenburg and S. Infantis. From each serovar, isolates with different biofilm forming capacity were selected to analyse biofilm formation on stainless steel. This revealed no significant differences between biofilm formation on polystyrene compared to stainless steel. Furthermore a relation was observed between biofilm forming capacity of an isolate and survival on stainless steel surfaces. On such surfaces, biofilms showed greater and longer survival than planktonic cells, and they were less susceptible to peracetic acid disinfection treatments. However, the latter effect was marginal and only observed in the presence of organic material, which drastically decreased the activity of peracetic acid. With the obtained results a hierarchical cluster was also performed to identify differences and similarities between the four different serovars. This indicated that the surface behaviour of S. Typhimurium was more comparable to S. Infantis than to S. Derby or S. Brandenburg. 相似文献
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V Bhardwaj 《Australian veterinary journal》2001,79(9):619-619
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Cross-protection induced by primary infection with Abortusovis and Gallinarum was examined against challenge injection with these Salmonella serotypes as well as with Dublin and Choleraesuis, the other virulent serotypes. Abortusovis induced efficient protection against the other Salmonella. Gallinarum was ineffective against Choleraesuis. Even with low multiplication in mice, the Gallinarum J91 strain induced a weak but significant protection against Dublin (same O group serotype). The antibodies in the blood of mice were tested with ELISA specific for the Salmonella antigens used to prime or to challenge animals. The Gallinarum J91 strain was detected to be more antigenic in ELISA than the other Salmonella antigens. It is difficult to conclude on a correlation between IgM or IgG antibodies and induction of protection, because of the variability in immune response according to the different serotype used. Nevertheless, the negative linkage between a number of bacteria in the spleen of mice challenged with Gallinarum and Dublin, and the level of IgM and IgG antibodies specific for the challenging serotype, showed that humoral immune response could be one element of cross-protection, mainly by the immune response against the same O serotype. 相似文献
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