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1.
野生胡子鲶“腹水病”病原分离和鉴定   总被引:8,自引:0,他引:8  
从发生“腹水病”的野生胡子鲶体内分离出14株致病菌,经生化鉴定,有12株细菌(87%)定为豚鼠气单胞菌,1株定为大肠埃希氏菌。动物回归试验也证实了细菌的致病性。因此,本次野生胡子鲶发生“腹水病”主要病原是豚鼠气单胞菌,药敏试验结果对细菌有效的药物有头孢类抗生素、丁胺卡那霉素,卡那霉素,新霉素,氯霉素,庆大霉素。  相似文献   

2.
鱼源气单胞菌的主要特性研究   总被引:7,自引:0,他引:7  
对分离于草鱼肠炎、白鲢鱼打印病、鲢鱼白皮病、草鱼烂鳃、鲤鱼体表出血感染及甲鱼败血症等病例的80株细胞进行了主要特性研究。结果表明均为气单胞菌属(Aeromonas)的细菌,其中嗜水气单胞菌(A.hydrophila)29株(占36.25%)、豚鼠气单胞菌(A.caviae)38株(占47.5%)、温和气单胞菌A.sobria)13株(占16.25%)。在被检验的病例中,或为其中一种气单胞菌的单独感染,或为两种、三种气单胞菌的混合感染。经做人工感染试验检查,表明这些分离菌均为相应感染症的致病菌。  相似文献   

3.
甲鱼暴发性传染病的病原分离及治疗试验   总被引:5,自引:0,他引:5  
从15例表现为“红底板”、“红脖子”、“烂甲”的患病甲鱼肝脏分离到9株无荚膜、无芽胞、革兰氏阴性、β溶血的短杆菌,经理化特性鉴定均为气单胞菌属(Aeromonas)嗜温有动力菌群,其中温和气单胞菌(A.sobria)7株,豚鼠气单胞菌(A.caviae)2株。从A.sobria和A.caviae各选一代表菌株(JA-1和JA-2)分别感染健康鲫鱼均引起发病和死亡,其LD50分别为4.3×105、5.5×107,并回收到该2种菌。在该2种菌培养上清中存在1种具有溶血活性的毒素。以JA-1和JA-2为菌种制成混合菌苗,在应用由黄芪、有机锗制成的免疫增效剂后,再用混合菌苗免疫家兔,制得的多价高免血清(效价达1∶512)与庆大霉素、恩诺沙星进行了治疗对比试验。结果,高免血清治疗自然病甲鱼109只,全部治愈;庆大霉素、恩诺沙星分别治疗自然病甲鱼72只和84只,平均治愈率分别为88%和84%。  相似文献   

4.
24种抗菌药物对鳖气单胞菌的抑菌试验   总被引:2,自引:0,他引:2  
从北京市某养殖基地发病死亡的鳖内脏组织中,分离到20株气单胞菌,其中嗜水气单胞菌7株,温和气单胞菌12株,豚鼠气单胞菌1株,用24种抗菌药物对其进行药敏试验结果,头孢三嗪,头孢噻肟,头孢呋新和头孢哌酮对该菌高度敏感。  相似文献   

5.
甲鱼病原性豚鼠气单胞菌的分离鉴定   总被引:13,自引:1,他引:12  
自杭州市郊某甲鱼场送检的病死甲鱼中分离到22株细菌,在与嗜水气单胞菌抗血清不产生凝集的多株细菌中挑选3株作进一步鉴定,生理生化特性显示它们均属豚鼠气单胞菌。小鼠试验显示有毒力,用其中1株回归本动物发病死亡,并出现与自然病例类似症状。用1株分离菌制备的抗血清与其他21株分离菌株进行玻板凝集试验,其中11株有毒力菌株都出现凝集反应,显示豚鼠气单胞菌为本病的主要病原。  相似文献   

6.
对市售的6种淡水鱼共采肠内容物样品73份,检出气单胞菌32株,阳性率为43.84%;类志贺邻单胞菌17株,阳性率为23.29%。其中有8份样品同时检出上述两菌。对32株气单胞菌用8项生化试验鉴定为4个种,分别是嗜水气单胞菌16株,豚鼠气单胞菌8株,温和气单胞菌7株,简达气单胞菌1株。  相似文献   

7.
边贸入境黄鳝鱼,活蟹气单胞菌的分离与鉴定   总被引:3,自引:2,他引:1  
从广西凭祥浦寨边贸点入境的越南黄鳝鱼,活蟹分离出8株细菌,生理生化特性显示它们的属气单胞菌,取纯培养物接种于葡萄糖发酵管,并做七叶苷水解试验,其中2株发酵葡萄糖但不产气,定为豚鼠气单胞菌(Aeromonascaviac)2株发酵葡萄糖产酸产气但不水解七叶苷,定为温和气单胞菌(Aeriminassobrla)4株发酵葡萄产糖产气且水解七叶苷,定为嗜水单胞菌(Aeromonashydrophila)。  相似文献   

8.
2020年5月,江苏省某养殖场饲养的220只山羊疑似感染产气荚膜梭菌,共死亡25只。为鉴定感染的产气荚膜梭菌复合群类型,采集大肠、小肠内容物以及肠系膜淋巴结、肝、脾、肾等组织病料进行细菌分离鉴定,并对分离菌株进行药敏试验及致病性试验。结果显示:分离菌株为革兰氏阴性短杆菌,经生化试验和16S rRNA试验鉴定为豚鼠气单胞菌;该分离株对多种常用抗菌药物耐药,仅对呋喃唑酮敏感;该菌能造成小鼠发病死亡。此次从临床发病羊病料中分离到致病性豚鼠气单胞菌,在国内较为少见,截至目前未见类似报道,本研究为避免此类疫病的临床误诊以及做好该病防控提供了帮助。  相似文献   

9.
江苏某养殖场种鸭突然发生散发性死亡,为了探究其死亡原因,本研究通过临床解剖观察、病原分离、生化试验、药敏试验、雏鸭人工感染试验和致病性试验对分离菌株进行检测。结果显示该分离菌为革兰阴性短杆菌,在麦康凯培养基和LB培养基中呈现圆形灰白色菌落,在血琼脂培养基中呈现出β溶血;16S rRNA基因序列分析结果显示该菌为维氏气单胞菌(A.veronii);药敏结果显示该菌对头孢氨苄、氨苄西林等10种抗生素敏感;人工感染试验显示该菌可造成雏鸭的死亡;小鼠的致病性试验显示该菌对小鼠的LD50为5.0×10^6.5cfu。总之,本实验从病鸭中分离到1株A.veronii,并筛选出敏感药物,为A.veronii病的诊断和治疗提供了参考依据。  相似文献   

10.
草鱼肠炎的病原菌检验与分析   总被引:7,自引:1,他引:6  
对2个鱼场的5尾病鱼的肠内容物及肝进行细菌学检查,在其中2尾病死鱼及1尾病鱼的肝中发现形态特征一致的细菌并分别到菌落特征基本一致的细菌在5尾鱼的肠内容物中均发现并分离到几乎纯一的肝中相同的细菌经对24纯培养菌理化特性鉴定,初步表明分离菌为气单胞菌属的哮敢单胞菌、温和气单胞菌及豚敢单胞菌,其中以嗜水气单胞菌占优势。回归试验表明,分离鉴定的3种气单胞菌均有致病性。  相似文献   

11.
In this study we describe biochemical, toxigenic and surface characteristics of 33 motile Aeromonas isolated from diseased mammals, 3 from moribund marine mammals, 24 from healthy fish and 4 from moribund fish. Aeromonas hydrophila, A. caviae and A. sobria were isolated from both mammals and fish but at a different incidence. Aeromonas hydrophila was the predominant species isolated from clinical specimens; it was isolated from pneumonia, wound infections, septicemia and abortion in horses, cattle and pigs. Aeromonas sobria was isolated from one mammal and 11 healthy fish. Aeromonas caviae was isolated in 2 cases from healthy fish and in 9 cases from diseased mammals. Variations in some biochemical tests including sorbitol, amylase and citrate, were observed between isolates from different sources. However, these differences did not allow the differentiation of isolates from diseased mammals and healthy fish. The majority of A. hydrophila isolates produced different extracellular products; A. sobria isolates produced less exotoxin. With A. caviae isolates no hemolysin, protease, enterotoxin or elastase were detected. There was no quantitative difference in hemolysin, protease, enterotoxin or elastase production between isolates from mammals and fish. It is suggested that A. hydrophila could be a potential pathogen for domestic animals, and fish may represent a potential reservoir of infection.  相似文献   

12.
Aeromonas sobria is an important bacterial pathogen of soft-shelled turtles (Trionyx sinensis). The effect of an oral alginate microparticle A. sobria vaccine (MAV) on immune response and protection efficacy of soft-shelled turtles was investigated. The formalin-killed cultures of A. sobria strain Z-1 (FKC) were incorporated into alginate microparticles, and the encapsulated vaccine was orally used for immunizing turtles. The serum agglutinating antibody titers, bactericidal activity of blood leucocytes and relative percent survival (RPS) post-challenge with virulent live bacteria of control and immunized turtles were detected. The serum agglutinating antibody titers and bactericidal activity of the leucocytes in the turtles orally immunized with MAV reached a level equivalent to those of the turtles injected with FKC and were significantly higher than those of control group. The RPS of turtles immunized orally with the MAV and intraperitoneally with FKC was 89.5 and 94.7%, respectively, while 95% of the control turtles had died. These results demonstrated that oral alginate microparticle A. sobria vaccine was immunogenic, and could confer protective immunity in soft-shelled turtles.  相似文献   

13.
中华鳖对温和气单胞菌口服微球缓释疫苗的免疫应答   总被引:3,自引:0,他引:3  
以中华鳖温和气单胞菌 (Aeromonas sobria,As) Z- 1株灭活全菌液 ,采用生物降解性高分子材料制成缓释微球疫苗 ,口服免疫中华鳖 ,测定血清中凝集抗体、血液中白细胞杀菌率以及对活菌攻击的免疫保护率。结果表明 ,中华鳖口服微球疫苗 ,其血清中凝集抗体效价和血液中白细胞杀菌百分率均可达到灭活菌液注射组相当的水平 (P>0 .0 5 ) ,显著高于对照组 (P<0 .0 1) ;微球疫苗口服组和灭活菌液注射组的免疫保护率分别为 94 .7%和 89.5 % ,两者差异不显著 (P>0 .0 5 ) ,而对照组小鼠 95 %死亡。采用可生物降解微球作为中华鳖气单胞菌口服疫苗的载体系统是可行的。  相似文献   

14.
嗜温气单胞菌的鉴定及其外膜蛋白型研究   总被引:1,自引:0,他引:1  
从病鳖、鳗鱼体内分离到2株嗜温气单胞菌(TAh-3、EAs-1),经形态学观察、生理生化特性等鉴定,确定TAh-3为嗜水气单胞菌,EAs-1为温和气单胞菌。动物感染试验结果表明,TAh-3株为致病性嗜水气单胞菌,对鲫鱼的致死率100%,EAs-1株为非致病性温和气单胞菌。SDS-PAGE电泳结果显示,TAh-3株外膜蛋白(OMPs)型由7条蛋白带组成,其中1条主带大小为40.0kDa;EAs-1株OMPs型由8条蛋白带组成,其中2条主带大小为38.0kDa、45.0kDa。  相似文献   

15.
The presence of potentially human pathogenic strains of Aeromonas was investigated in 84 samples of seafood which were purchased from retail traders in Berlin, Germany in spring 2000. A total of 134 Aeromonas strains were isolated on selective [GSP agar and Aeromonas (Ryan) agar] and unselective (standard count agar and enterohaemolysin agar) media from 27 (32.1%) of the samples and were classified as Aeromonas hydrophila (67.9%), A. caviae (26.1%) and A. sobria (6.0%) by biotyping. Thirteen (48.1%) of the 27 positive samples contained more than one species of Aeromonas. Production of haemolysins on enterohaemolysin agar was found with 132 (98.5%) of the strains at 28 degrees C and with 130 strains (97.0%) at 37 degrees C growth temperature. Vero cytotoxins were produced by 99 (73.9%) of the strains when grown at 28 degrees C but only by 24 of the strains (17.9%) at 37 degrees C. The latter strains were identified as A. hydrophila (n = 22) and A. sobria (n = 2) which came from 17 (20.2%) samples of raw seafood and from ready-to-eat salted herring 'Matjes' products. Cytotoxin-encoding genes for aerolysin (aer) and haemolysin A (hlyA) were investigated by PCR. Aer and hlyA genes were detected in both, strains which produced toxins only at 28 degrees C and strains which produced toxins at 37 degrees C. Our data indicate that raw seafood and ready-to-eat fish products can harbour potential human pathogenic, cytotoxin producing Aeromonas strains.  相似文献   

16.
In this study a total of 140 broiler carcasses and carcass parts purchased at different supermarkets in Ankara including 50 whole carcass, 30 wing, 30 leg and 30 breast samples were analysed for the presence of motile Aeromonas species. According to analysis findings, motile Aeromonas spp. were isolated from 116 (82.9%) of total 140 samples. The distribution of the isolates were 94%, 86.6%, 80%, 63.3% in broiler carcass, wing, leg and breast samples, respectively. Aeromonas hydrophila was isolated the most prevalent species with 56% the range followed by Aeromonas sobria with 29.3% and Aeromonas caviae with 14.7% from all of the carcass and carcass part samples, respectively. Consequently, it was supposed that, examined broiler carcass and carcass parts have been contaminated to important level with motile Aeromonas species and it has been risk for public health.  相似文献   

17.
两株气单胞菌的分离与鉴定   总被引:2,自引:0,他引:2  
从福建省某牛蛙养殖场病死牛蛙和某鸭场病死鸭体内分离到两株细菌,检验结果表明,两株分离菌均为有运动力的革兰氏阴性杆菌。根据其临床症状、细菌培养特性、生化特性、动物回归试验以及溶血试验等一系列试验,研究证明牛蛙感染的病原为嗜水气单胞菌,鸭感染的病原为温和气单胞菌。  相似文献   

18.
We tried to identify the bacteria and explore the mechanism of the bacteria's pathogenicity via housekeeping gene gyrB and in vitro organ culture (IVOC) of ileum and intramuscular injection. Microscope, electron microscope and scanning electron microscope were also used to observe and the structure, pathogen of M12 and changes of infected tissues. The results showed that M12 was short gram-negative bacteria, and both ends of it were obtuse and the size without flagellum was (0.6 to 1.6) μm×(0.6 to 0.7)μm while length of flagellum was about 2 to 3 times of the length of bacteria.It had 100.0% similarity with Aeromonas caviae from GenBank. IVOC test observed M12' adhesion in intestinal epithelial cell, resulting in formulation of biofilm structure and damage in intestinal epithelial cell. Histopathologic examination showed that M12 could cause damage in intestine, liver, lung, kidney, muscle tissue of rabbit.The assay would offer references for researching the pathogenic mechanism of Aeromonas caviae.  相似文献   

19.
Enteropathogenicity of Plesiomonas shigelloides, Aeromonas hydrophila, A. caviae and A. sobria was studied both in monoinfections and in coinfections with coccidium Cryptosporidium parvum in neonatal BALB/c mice. In monoinfection experiments, neonatal BALB/c mice were orally infected with 7 x 10(7) or 7 x 10(8) CFU, respectively, of a strain of P. shigelloides or a strain of an Aeromonas spp. In coinfection experiments, the neonatal mice were, in addition to being orally infected with one of the four bacterial species, orally infected with an inoculum containing 10(5) oocysts of C. parvum. Results from monoinfections with P. shigelloides revealed long-term colonisation of the neonatal mouse intestine by this pathogen, along with associated pathological lesions. The lesions varied in severity from atrophy to necrosis of the mucosal inner surface of the ileum and colon, with predilection to the colon and brush border of colonic enterocytes. The effects of coinfection of P. shigelloides with C. parvum were characterised by bacteremia and heavy colonisation of the intestine by P. shigelloides. In addition, extensive necrotising inflammatory changes in the ileum and colon were accompanied by diarrhoea and deaths of coinfected mice. In contrast, the results from monoinfections of neonatal mice with Aeromonas spp. showed only a short-term colonisation of the intestine by the pathogen. However, when mice were coinfected with A. hydrophila and C. parvum, then the growth of the bacterial species was prolonged, and occurred in both the spleen and intestine. However, no substantial clinical or histopathological changes were observed in mice, whether monoinfected with Aeromonas spp. or coinfected with C. parvum. Our study suggests that experimental monoinfections of neonatal BALB/c mice with P. shigellodes, Aeromonas spp. and C. parvum, together with coinfections (each bacterial species with the protozoan C. parvum), may serve as a useful model to study the initial steps of gastrointestinal colonisation and diarrhoeal disease syndromes caused by enteropathogenic bacteria and protozoa, individually and in combination.  相似文献   

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