首页 | 本学科首页   官方微博 | 高级检索  
相似文献
 共查询到20条相似文献,搜索用时 93 毫秒
1.
单克隆抗体在动物衣原体病血清诊断上的应用   总被引:2,自引:0,他引:2  
采用淋巴细胞杂交瘤技术,研制出的抗衣原体单克隆抗体,应用酶联免疫吸附试验(ELISA)方法,对本地区所采得的231份猪血清,128份羊血清,90份牛血清进行检测,结果:猪血清阳性检出率为30.7%,羊为25.8%,牛为32.2%;选猪、羊、牛血清各50份,用已建立的检测衣原体抗体ELISA方法与的间接血凝试验(IHA)进行比较,猪血清ELISA阳性检出率为30%,IHA为26%,ELISA比IHA试验高出4个百分点。牛血清ELISA阳性检出率为32%,IHA为24%,ELISA比IHA试验高出8个百分点。羊血清ELISA阳性检出率为26%,IHA为22%,ELISA比IHA高出4个百分点。  相似文献   

2.
本试验旨在迅速、准确检测口蹄疫阴性血清和阳性血清,确立O型口蹄疫抗体金标检测试纸条的判定标准。通过3批次O型口蹄疫抗体金标检测试纸条,对180份猪、牛、羊口蹄疫阴性血清,197份猪、牛O型口蹄疫弱阳性血清,317份猪、牛、羊O型口蹄疫阳性血清,223份牛Asia 1、A型口蹄疫阳性血清,600份田间血清样品进行检测,同时用O型口蹄疫液相阻断ELISA和O型口蹄疫正向间接血凝2种方法检测相同的血清样品。对3种方法检测的结果进行分析,并且将试纸条的检测线和质控线的显色情况与O型口蹄疫液相阻断ELISA的抗体滴度相比较。确立了试纸条的诊断标准:试纸条抗体滴度1∶2-为口蹄疫抗体阴性;试纸条抗体滴度1∶8+为O型口蹄疫阳性血清。根据试纸条的显色结果与液相阻断ELISA的结果的关系,绘制了比色卡。试纸条的判定标准检测血清结果与液相阻断ELISA和正向间接血凝结果分别进行统计学分析,试纸条与液相阻断ELISA和正向间接血凝相关性分别为y=1.142x+0.7421,y=1.1845x+0.8623;相关系数(r)分别为0.9221和0.9033。结果显示,O型口蹄疫抗体金标检测试纸条的判定标准的确立和比色卡的绘制,实现半定量分析,更加迅速、准确,适于实验室、基层兽医站和养殖场使用。  相似文献   

3.
通过正向间接血凝试验和酶联免疫吸附试验(阻断ELISA)对3个规模化猪场提供的66份血清样品进行猪瘟免疫效果平行对比检测。结果:66份血清样品用正向间接血凝试验检测免疫合格为65份,免疫合格率为98%;阻断ELISA法检测免疫合格为66份,免疫合格率为100%。  相似文献   

4.
采用淋巴细胞杂交瘤技术,研制出的抗衣原体单克隆抗体,应用酶联免疫吸附试验(ELISA)方法,对本地区所采用的698份羊血清进行检测,羊血清阳性检出率为22.06%,选择50份羊血清用已建立的检测衣原体抗体ELISA方法与传统的间接血凝试验(IHA)进行比较,其敏感性高。羊血清ELISA阳性检出率为26%,IHA为22%,前者比IHA高出4个百分点。  相似文献   

5.
用血凝抑制试验和间接ELISA两种方法检测乙脑阳性血清均呈阳性反应,检测乙脑阴性血清均呈阴性反应。用间接ELISA对来自9个猪场74份送检血清进行了猪乙脑病毒(Japanese encephalitis virus,JEV)抗体检测,并与血凝抑制试验(HI)进行对比,两种方法检测总符合率为85.1%(63/74),经统计分析,检测结果差异不显著(P>0.05)。对来自无猪乙脑病猪场24头健康猪的血清进行检测,两种方法结果均为阴性,符合率为100%。对10份阳性血清进行检测,ELISA检测效价较HI效价高,表明ELISA比HI敏感。结果表明,ELISA与HI检测结果相符,前者更适用于猪血清中乙脑IgG抗体的大规模检测。  相似文献   

6.
以纯化的牛分枝杆菌重组MPT83蛋白为包被抗原,建立了检测牛分枝杆菌抗体的间接ELISA方法。确定了间接ELISA各组分的最适反应条件:抗原包被浓度为1μg/mL,酶标二抗稀释度为1:1600,血清稀释度为1:60,抗原和血清、血清和二抗均在37C反应30min,底物在37℃显色15min,D655nm阴性、阳性临界值为0.5。经阻断试验、交叉试验、重复性试验,表明该方法特异性强、重复性好。用该方法对18份结核菌素试验阳性牛血清和36份结核菌素试验阴性牛血清进行检测,结果显示,阳性血清的符合率为27.8%,阴性血清的符合率为91.7%。  相似文献   

7.
分别采用商品化的酶联免疫吸附试验(ELISA)抗体检测试剂盒和血凝抑制(HI)试验方法对152份临床血清样本和标准阴阳性血清进行新城疫病毒(NDV)抗体检测。比较间接ELISA和HI两种方法的相关性。研究结果表明,间接ELISA和HI两种方法呈显著相关,二者间的相关系数为0.9261:间接ELISA和HI两种方法检测NDV抗体的阳性符合率为96.8%,阴性符合率为92.9%,间接ELISA方法是一种敏感、特异、快速的血清学诊断方法,可以用于临床样本的大规模检测.  相似文献   

8.
甘肃省部分猪场猪衣原体血清抗体的检测   总被引:6,自引:0,他引:6  
用间接血凝试验对甘肃省7个市(地)的部分猪场猪群用间接血凝试验进行衣原体血清抗体检测。共检测468份猪血清,阳性161份,可疑51份,总阳性率为34.40%,其中武威市为66.67%,平凉市为56.25%。  相似文献   

9.
分别用血凝抑制试验(HI)和酶联免疫吸附试验(ELISA)检测42周和48周SPF鸡血清、以及同期SPF鸡蛋蛋黄中新城疫(ND)抗体。结果表明,两种方法检测的120份血清中均无ND抗体,呈阴性;120枚SPF鸡蛋蛋黄检测,ELISA方法检测结果全为阴性;HI方法检测结果25份阳性,差异较为明显。  相似文献   

10.
对采自西宁地区两个绪场的76份血清用正向间接血凝试验进行了猪瘟免疫抗体的检测。结果检出阳性59份,抗体保护率为77.63%(59/76)。  相似文献   

11.
12.
本文概述了猪的毛色类型、猪的毛色遗传模式,着重综述了猪毛色基因分子基础的研究进展,指出存在问题并就未来发展方向做了思考。  相似文献   

13.
在现代法律秩序中,商会自治规范是制定法的基础和必要的补充,甚至在某些方面替代了制定法;商会自治规范主要包括商会组织规范、行为规范、惩罚规范以及争端解决规范等;其效力仅及于其内部成员;商会自治规范和制定法之间存在冲突,但也存在整合的基础。  相似文献   

14.
REASONS FOR PERFORMING STUDY: Centesis of the bicipital bursa using an 8.9 cm long spinal needle has been reported but the alternative of employing a 3.8 cm long hypodermic needle requires validation. OBJECTIVE: To compare the efficacy of 2 different methods of centesis of the bicipital bursa and to evaluate the usefulness of ultrasonographic imaging to determine the location of solution administered when centesis of the bursa is attempted. METHODS: For Trial 1, 6 clinicians, who had no previous experience of centesis of the bicipital bursa, attempted to inject a solution composed of an aqueous radiopaque contrast medium and physiological saline solution (PSS) into the bicipital bursae of 2/12 horses using the previously described distal approach to inject one bursa and a proximal approach to inject the contralateral bursa. The bicipital tendon and bursa were examined ultrasonographically before and after injection; and both shoulders were examined radiographically to identify the location of the medium. In Trial 2, another 6 clinicians, also with no previous experience of centesis, repeated Trial 1, using 6 horses, but the radiopaque contrast medium was mixed with air instead of PSS. RESULTS: Accuracy of centesis using the proximal approach was 39% and that of the distal approach 28%. Ultrasonographic examination of the shoulder allowed the location of solution and air to be accurately predicted in all 12 shoulders examined. CONCLUSIONS: Clinicians who have had no previous experience performing centesis of the bicipital bursa are unlikely to be successful in centesis using either approach. Radiographic examination after injecting a radiopaque contrast medium may be necessary to assess the success of centesis especially if bursal fluid is not obtained during centesis. Injecting air along with the radiopaque contrast medium provides more accurate ultrasonographic confirmation of centesis and better radiographic definition than does injection without air.  相似文献   

15.
Ingestively masticated fragments were collected and sized via sieving. Different sizes of esophageal masticate and ruminal digesta fragments, and ground fragments of larger masticated pieces were incubated in vitro, and undigested NDF remaining at intervals of up to 168 h of incubation was determined. The ruminal age-dependent time delay (tau) for onset of digestion of NDF was positively correlated (P < 0.004) with the mean sieve aperture estimated to retain 50% of the fragments between successive sieve apertures (MRA). Degradation rate of potentially degradable NDF (PDF) and level of indigestible NDF were not related (P > 0.10) to MRA of masticated and ground fragments. Estimates of tau were positively related to MRA, with slopes of bermudagrass < corn silage < ruminal fragments of corn silage. It was concluded that fragment size-, and consequently, ruminal age-dependent onset of PDF degradation of a mixture of different fragment sizes results in an age-dependent rate of degradation of the more rapidly degrading of two subentities of PDF. Models are proposed that assume a tau before onset of simultaneous degradation of PDF from two pools characterized as having gamma-modeled age-dependency and age-constant rates. The ruminal age-dependent pool seems to be associated with the faster-degrading pool, and its rate parameter increases with range in MRA in the population of fragments. Conceptually, the ruminal age-dependent rate parameter for PDF degradation seems to represent a composite of several effects: 1) effects of the size-dependent tau; 2) range in MRA of the population of ingestively masticated fragments; and 3) subentities of PDF that degrade via more rapid age-dependent rates compared with subentities of PDF that degrade via age-constant rates. The estimated fractional rates of ruminative comminution of ingestively masticated fragments (0.060 to 0.075/h) were of a magnitude similar to the mean fractional rates of PDF digestion (0.030 to 0.085/h), which implies that ruminative comminution may be first-limiting to fractional rate of PDF digestion. The in vivo roles of ingestive and ruminative mastication of fragments on PDF degradation must be considered in any kinetic system for estimating PDF digestion in the rumen. These results and others in the literature suggest that the rate of surface area exposure rather than intrinsic chemical attributes of PDF may be first-limiting to degradation rate of PDF in vivo.  相似文献   

16.
用硝酸和高氯酸消化蜂蜜,使硒游离出来,在微酸性环境下,硒和2,3-二氨基萘(DAN)生成有较强荧光的物质,用环己烷萃取,在激发波长378nm,荧光波长518nm处测定其荧光强度。蜂蜜中硒含量范围:0.10~0.82μg/g。表明:蜂蜜应视为天然富硒营养品。  相似文献   

17.
Fractures of the anconeal process of 5 pigs ranging in age from 4 to 8 months were studied radiographically and histologically. Clinically, animals with a fracture of the anconeal process had a "tight," restricted gait. In pigs at 4.5 months of age, a radiolucent line through the base of the anconeal process was composed of fibrocartilage, fibrous connective tissue, and hyaline cartilage. Subperiosteal proliferation of woven bone was located along the cranial surface of the olecranon, adjacent to the base of the anconeal process. In older animals, the radiolucent line through the anconeal process contained variable amounts of fibrous connective tissue and fibrocartilage. The proliferation of subperiosteal bone at the base of the anconeal process formed a "buttress callus" which retained a radiolucent area between the callus and the proximal surface of the anconeal process. The latter region of radiolucency was continuous with the transversely oriented line that traversed the base of the anconeal process.  相似文献   

18.
为贯彻落实《兽药生产质量管理规范》(简称《兽药GMP》),进一步推动兽药GMP实施进程,我部制定了《兽药生产质量管理规范检查验收办法》,现予公告。本公告自2003年6月1日起施行。附件:兽药生产质量管理规范检查验收办法二○○三年四月十日第一章 总则 第一条 为推动《兽药生产质量管理规范》(以下简称兽药GMP)的实施,规范兽药GMP检查验收工作,制定本办法。 第二条 农业部负责全国兽药GMP管理和检查验收工作;负责制修订兽药GMP检查验收管理规定;负责兽药GMP检查员队伍建设和监督管理工作,负责国际兽药贸易中GMP互认工作。 …  相似文献   

19.
以国际标准强毒R株人工感染非免疫产蛋鸡,定时扑杀,分别从鼻窦、眶下孔、气管、肺、气囊、卵巢和输卵管分离MG,并收集感染鸡所产蛋分离MG。结果表明,人工感染48小时后上、下呼吸道及肺已被全面感染,96小时气囊已被感染,120小时输卵管已能分离到MG,卵巢始终分离不到MG。人工感染鸡自144小时便能在其所产蛋中分离出MG。药物治疗能在72小时内消除感染,油乳剂苗则需24天后逐渐降低蛋内MG分离率,药物卵内注射、种蛋药浴、高温处理均能杀死卵内MG,但以研制的种蛋浸泡剂药浴效果为最好。  相似文献   

20.
乳酸杆菌益生作用机制的研究进展   总被引:2,自引:0,他引:2  
乳酸杆菌作为益生菌广泛用于人和动物。本文综述了乳酸杆菌改善宿主健康的机制。乳酸杆菌可通过产生抗菌物质如乳酸、过氧化氢、细菌素,或者通过竞争营养或肠道黏附位点来抑制致病菌;通过诱导黏附素的分泌或阻止细胞凋亡而增强肠道的屏障功能,从而保护肠道。文章重点讨论了乳酸杆菌表面成分(表面蛋白、脂磷壁酸和肽聚糖)与肠道受体(C型凝集素受体、Toll样受体和 Nod样受体),阐述了他们结合后启动免疫调节信号,调控肠道免疫功能以发挥改善健康作用的机制。  相似文献   

设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号