共查询到17条相似文献,搜索用时 171 毫秒
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功能基因组学研究进展 总被引:1,自引:0,他引:1
基因组研究计划包括以全基因组测序为目标的结构基因组学和以基因功能鉴定为目标的功能基因组学2方面的内容.作者对功能基因组的主要研究方法:微阵列或DNA芯片、表达序列标签法、基因表达系列分析法、蛋白质组学分析法以及反向遗传学技术等进行了简要介绍,同时综述了上述各种技术的优缺点以及部分生物功能基因组的研究进展. 相似文献
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真核细胞中染色质以不同层次的三维结构有序的折叠在细胞核中,其空间层次结构对基因的表达调控和细胞发挥正常的生理功能都起着重要的作用,在动物育种方面具有很大潜力,但尚未被完全探索。本文介绍三维基因组的结构单元(染色质疆域、A/B 区室、拓扑关联结构域和染色质环)以及研究三维基因组的主要技术,对三维基因组学在农业中的研究进展和应用前景进行了探讨,旨在深入了解三维基因组学的功能和应用前景,以期为生物育种改良提供部分帮助。 相似文献
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Completion of the pig genome sequencing will celebrate the Chinese Year of the Pig in 2007. The International Swine Genome Sequencing Consortium has established an integrated approach to create genome sequences, chromosomal contigs, whole-genome libraries, full-length cDNA libraries and to generate single nucleotide polymorphisms (SNPs). The project is being conducted in collaboration with The Wellcome Trust Sange Institute. Project information as well as monitoring progress of the project can be viewed at http://www.sanger.ac.uk/Projects/S_scrofa/. During the next year, numerous physical maps and functional genomics analyses will be published. These advances will have significant implications for studying digestive physiology. Examples illustrating as to how effective genomic approaches for addressing critical questions in digestive physiology are presented. This includes approaches showing how genomics can be used to monitor the process of GI colonization and the effects of various nutrients are explored. The development of comparative genome maps also permits the incorporation of experimental studies in other species (mice and humans) to identify new hypotheses for testing in the pig. This comparative genomics approach further supports the use of the pig in biomedical research studies for human clinical studies. As this new genomic information becomes available, the necessity to create interactive databases, that provide portals for using genomic information to create gene based hypotheses will become rate limiting. Thus, the digestive physiology community contributions towards building web-based interfaces are essential. 相似文献
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柞蚕功能基因研究进展 总被引:2,自引:1,他引:1
柞蚕是起源于中国的大型绢丝昆虫,保护和开发其基因资源对柞蚕业的可持续发展具有重要意义。根据GenBank数据库的检索结果,从能量相关蛋白基因、免疫相关基因、神经肽类基因、昼夜节律相关基因、气味结合蛋白基因、表皮蛋白基因、代谢相关酶类基因等方面介绍柞蚕功能基因分离研究进展,并提出了今后开展有关研究的对策和建议。 相似文献
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cDNA microarray gene expression analysis and toxicological phenotype for anticancer drug 总被引:1,自引:0,他引:1
Lee GJ Lee WS Jeon KS Um CH Kim YS Kim SJ Lee CH Yoon HK Hwang SY Park JS Hwang JW Kang KS Lee YS Kim MS Chon KJ Yeo CD Kang JS 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(11):1339-1345
Toxicogenomics, the subdiscipline that merges genomics with toxicology, hold the promise to contributing toward the goal of elucidating mechanism by studying genomic profiling related with various drugs. The application of gene expression profiling technology to examine multiple genes and signaling pathways promises a significant advance in understanding the toxic mechanisms of various drugs and prediction of new drug candidate. Toxicogenomics is emerging field combining genomics and bioinformatics to identify and characterize mechanisms of toxicity of drug and various compounds. The principal hypothesis underlying on this field is that chemical-specific pattern of altered gene expression is related with each chemicals properties, especially toxicological property, and it will be revealed using high-density microarray analysis of sample from exposed organisms. So, in this study we compare the gene expression pattern of two anticancer drugs paclitaxel and orally absorbable paclitaxel, using the cDNA microarray. And from the result of this study, it is possible to provide the new possibility for genome-wide insight into mechanism of their anticancer activity and toxicological phenotype. 相似文献
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旨在从基因组层面揭示梅花鹿与欧洲马鹿的起源进化,找到与进化过程相关的信号通路并与表型进行关联。本试验以成年雌性梅花鹿与成年雄性欧洲马鹿染色体水平基因组作为研究对象,利用比较基因组学的方法对梅花鹿和欧洲马鹿基因组进行染色体共线性分析,获得两个物种间基因组序列的同源关系和基因组发生的染色体倒位现象,并对被倒位截断和在倒位内部未被截断的两类基因分别进行GO和KEGG功能富集分析;同时对两个物种染色体上的基因进行共线性分析,识别二者基因组间的直系同源区域,检测直系同源基因,估算两个物种的分化时间。结果显示,梅花鹿与欧洲马鹿基因组表现出同源性,有27条染色体的同源性在95%以上;通过基因组的比对确定了梅花鹿的23号染色体是X染色体;而对染色体倒位的统计及基因的GO和KEGG功能富集分析发现,梅花鹿基因组共有37 847个倒位,片段长度为1~5 kb的倒位数目最多;而倒位涉及基因的GO功能富集的生物学过程及分子功能有嗅觉中化学刺激的检测、嗅觉感觉、嗅觉感受器活动、信号传感器活动;KEGG富集的是嗅觉传导信号通路。而梅花鹿与欧洲马鹿基因共线性分析共检测出79个同源区段,12 629个直系同源基因,利用同源基因的同义突变率(Ks)估算出梅花鹿与欧洲马鹿的分化时间是0.318 MYA (millions of years ago)。本研究利用比较基因组学的方法,获得了梅花鹿与欧洲马鹿基因组间的同源关系以及两个物种染色体倒位现象,通过功能富集分析发现,染色体倒位与嗅觉表型有关,为鹿属动物染色体进化的研究提供新的理论基础。 相似文献
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It has long been appreciated that animals fed the same diet may perform differently. This is due to the ability of nutrients to interact with and affect molecular pathways that result in differences in BW gain, production performance, or disease resistance. To understand these effects, studies are being undertaken to discover how the differential expression and function of genes occur with different diets. These studies are using new technologies, genomic resources, and analysis techniques that have recently become available for domestic animals. Nutrigenomics and nutrigenetics are new research approaches that strive to optimize health by looking beyond the diet to understand the effects of food at the genetic and epigenetic levels. Nutrigenomics is focused on the effects of diet on health through an understanding of how bioactive chemicals in foods and supplements alter gene expression or the structure of the genome of an animal. Nutrigenetics focuses on how the genetic composition (i.e., genetic variation) of an animal influences their response to a given diet. Results from these studies will aid in formulating nutritionally appropriate diets that may be optimized for animals based on their genomic underpinnings. Nutrigenomics and nutrigenetics unite many fields: nutrition, bioinformatics, molecular biology, genomics, functional genomics, epidemiology, and epigenomics. The use of multi-disciplinary tools promises new opportunities to investigate the complex interactions of the genome and the diet of an animal. Through these new approaches, the partnerships of the genome and nutrition will be revealed resulting in improved efficiency of diets, enhanced sustainability of animals as a protein source, and improved methods for preventing illnesses. 相似文献