紫外分光光度法测定伊维菌素片的含量

用紫外分光光度法测定伊维菌素片中伊维菌素B1的含量。伊维菌素溶于甲醇 ,而辅料不溶 ,过滤分离出伊维菌素 ,于 2 0 0～ 4 0 0nm间扫描 ,在 2 38nm、2 4 5nm波长处有最大吸收峰。以2 4 5nm作检测波长 ,伊维菌素在 4～ 2 8μg /ml范围内与吸收度呈良好线性关系。用伊维菌素作对照品测得伊维菌素片的平均回收率为 10 0 13% ,RSD为 0 2 5 % ,n =5。紫外分光光度法具有快速、准确、易操作等优点。该方法对市场快速检测及生产厂家质量监控具有实用价值。     

紫外分光光度法测定伊维菌注射液中伊维菌素B1的含量

为用紫外分光光度法测定伊维菌注射液中伊维菌素B1的含量,将伊维菌素注射液溶于甲醇,于200～400nm间扫描,在238nm和245nm波长处有最大吸收峰,245nm波长处测定伊维菌素对照品,测得伊维菌素注射液的平均回收率为100.06%,RSD为0.24%(n=5),与《中国兽药典》规定方法比较,结果基本一致,且具有快速、准确、易操作等优点。该方法对市场快速检测及生产厂家质量监控具有实用价值。     

紫外分光光度法快速测定伊维菌素注射液的含量

采用紫外分光光度法测定伊维菌素注射液的含量,于220～360 nm间扫描,在238 nm、245 nm波长处有最大吸收峰.实验结果表明,在波长为245 nm处,伊维菌素在4～28μg/mL范围内呈良好的线性关系.本法的回收率为100.1%,RSD为0.30%,n=5.本法具有简便、快速、易操作等优点,对市场快速检测及生产厂家质量监控具有实用价值.     

紫外分光光度法测定伊维菌素预混剂的含量

伊维菌素溶于甲醇,而辅料不溶,过滤分离出伊维菌素,于200－400nm间扫描,在238nm、245nm波长处有最大吸收峰。以245nm作检测波长,伊维菌素在4-28μg/ml范围内与吸收度呈良好线性关系。用伊维菌素作对照品测得伊维菌素预混剂的平均回收率为99.94%,RSD＝0.24%,n=5。紫外分光光度法具有快速、准确、易操作等优点,该方法对市场快速检测,生产厂家质量监控具有实用价值。     

紫外分光光度法快速测定伊菌素注射液的含量

采用紫外分光光度法测定伊维菌素注射液的含量,于220－360nm间扫描,在238nm、245nm波长处有最大吸收峰。实验结果表明,在波长为245nm处,伊维菌素在4－28μg/mL范围内呈良好的线性关系。本法的回收率为100.1%,RSD为0.30%,n=5。本法具有简便、快速、易操作等优点,对市场快速检测及生产厂家质量监控具有实用价值。     

伊维菌素注射液含量的测定法

用紫外分光光度法测定伊维菌注射液中伊维菌素B1的含量。伊维菌素注射液溶于甲醇,于200~400nm间扫描,在238nm、245nm波长处有最大吸收峰,245nm波长处测定吸伊维菌素作对照品测得伊维菌素注射液的平均回收率为100.06%,RSD为0.24%(n=5),与《中国兽药典》规定方法比较,结果基本一致,且具有快速、准确、易操作等优点。     

紫光分光度法测定伊维菌素预混剂的含量

伊维菌素溶于甲醇,而辅料不溶,过滤分离出伊维菌素,于200～400nm间扫描,在238nm、245nm波长处有最大吸收峰.以245nm作检测波长,伊维菌素在4～28μg/ml范围内与吸收度呈良好线性关系.用伊维菌素作对照品测得伊维菌素预混剂的平均回收率为99.94%,RSD=0.24%,n=5.紫外分光光度法具有快速、准确、易操作等优点,该方法对市场快速检测,生产厂家质量监控具有实用价值.     

紫外分光光度法测定泰乐菌素注射液含量方法的建立

以0.1 mol/L盐酸溶液为参比液,采用紫外分光光度法在290 nm波长处测定泰乐菌素注射液中泰乐菌素的含量.结果表明,泰乐菌素在5 U/mL～50 U/mL浓度范围内,其吸光度与浓度呈良好的线性关系,相关系数r=0.999 2,平均回收率为99.6%,RSD为0.4(n=6).该方法操作简便、快速、准确,适于作为生产企业中问产品的质量控制方法.     

紫外分光光度法测定水杨酸钠注射液含量

建立了水杨酸钠注射液紫外分光光度法 ,在波长 2 95.5± 1 nm处测定水杨酸钠含量 ,在3.7～ 60 .0μg/ml范围内线性关系良好 ,R=0 .9999。本方法具有快速、简便、准确等优点     

紫外分光光度法测定阿维菌素预混剂的含量

用紫外分光光度法测定阿维菌素预混剂中阿维菌素B1的含量。阿维菌素溶于甲醇,而辅料不溶,过滤分离出阿维菌素,于200－400nm间扫描,在238nm,245nm波长处有最大吸收峰,以245nm作检测波长,阿维菌素在4－28μg/mlL范围内与吸收率呈良好线性关系。用阿维菌素作对照品测得阿维菌素预混剂的平均回收率为100．05％,RSD为0．38％（n=5)。紫外分光光度法具有快速、准确、易操作等优点,该方法对市场快速检测,生产厂家质量监控具有实用价值。     

分光光度法检测饲料中汞含量

<正>目前,检测汞的含量主要采用冷原子吸收光度法,该法有较好的灵敏度和准确性,因而成为国家标准方法(GB13081—91)。但它需要特殊的仪器和装置,给汞的日常分析带来诸多不便。为了寻找一种简便的测汞方法,我们对分光光度法进行了详细的研究。     

系数倍率法检测伊维菌素脂质体含量的研究

伊维菌素脂质体是伊维菌素的一种重要新剂型,为消除辅料对伊维菌素含量测定的影响,采用系数倍率法在245nm和222nm处不经分离直接测定脂质体中伊维菌素的含量。结果表明,伊维菌素在4～28μg／mL范围内呈良好的线性关系,r=0．9999,平均回收率为104．6％。说明系数倍率法能消除脂质体中卵磷脂与胆固醇对伊维菌素含量测定的影响,可作为伊维菌素脂质体质量控制的有效方法。     

介绍一种新的抗菌活性测定方法

本文介绍胰大豆汤双层琼脂平板孔穴扩散法测定抗菌活性的配方、操作方法,该方法的灵敏度是琼脂平板孔穴扩散法的4～6倍。     

Evaluation of the wick catheter as used to measure intracompartmental muscle pressure in equine muscle

The use of the wick catheter to measure intracompartmental muscle pressure in equine muscle was documented. The presence of muscle compartments involving the extensor carpi radialis muscle and the long head of the triceps brachii was demonstrated by anatomic dissection and radiographic technique. The wick catheter was capable of accurately measuring pressures within both of these compartments. Furthermore, the wick catheter was sensitive to pressure changes resulting from external compression of muscle compartments. Manipulation of systemic blood pressure and PaCO2 in 1 anesthetized horse did not affect intracompartmental muscle pressure.     

Evaluation of the reproducibility and accuracy of pH-determining devices used to measure urine pH in dogs

OBJECTIVE: To evaluate the reproducibility and accuracy of 4 portable pH meters, a reagent strip, and pH paper for measuring urine pH in dogs. DESIGN: Prospective masked randomized study. SAMPLE POPULATION: 201 free-catch urine samples from 114 hospitalized dogs. PROCEDURES: Urine samples were divided into 2-mL aliquots. Measurements of urine pH were obtained by use of a laboratory benchtop pH meter, 4 portable pH meters, a urine reagent strip, and pH paper. The pH of each aliquot was measured within 4 hours of collection by an evaluator unaware of the aliquot's origin.To assess reproducibility, the coefficient of variation for each pH measurement device was calculated. To determine which device was most accurate, the degree of agreement among the different devices was assessed in comparison with the benchtop pH meter, which was considered the reference method. RESULTS: 3 of the 4 portable pH meters had nearly perfect agreement with the reference method. The reagent strip and pH paper had moderate to poor agreement with the reference method. CONCLUSIONS AND CLINICAL RELEVANCE: Urine pH measurements should be made by use of a portable or benchtop pH meter when accurate measurements are crucial for diagnosis or treatment. Reagent strips and pH papers are useful in obtaining pH approximations but are not recommended when accurate measurements of urine pH are required.     

Simplified method to measure glucocorticoid metabolites in faeces of horses

Glucocorticoids or their metabolites can be measured in several body fluids or excreta, including plasma, saliva, urine and faeces. In recent years the measurement of glucocorticoid metabolites (GCMs) in faeces has gained increasing attention, because of its suitability for wild populations. In horses, however, the group-specific enzyme immunoassay described so far has a limited practicability due to its complex extraction procedure. Therefore, we tested the applicability of other enzyme immunoassays for glucocorticoid metabolites. The present study clearly proved that an enzyme immunoassay (EIA) for 11-oxoaetiocholanolone using 11-oxoaetiocholanolone-17-CMO: BSA (3α,11-oxo-A EIA) as antigen showed high amounts of immunoreactive substances. Therefore it was possible to use just a small amount of the supernatant of a methanolic suspension of faeces. The results correlated well with the already described method for measuring GCMs in horse faeces, i.e. analysing the samples with an EIA after a two step clean up procedure of the samples (Merl et al. 2000). In addition, the 3α,11-oxo-A EIA has the advantage of providing a bigger difference between baseline values and peak values after ACTH stimulation. The new assay increased the accuracy of the test, lowered the expenses per sample, and storing samples at room temperature after collection was less critical than with other assays investigated in our study. This is a big advantage both in the field of wildlife management of equids and in the field of equestrian sports and it shows the importance of choosing an assay which is in good accordance with the metabolites excreted in a given species.     

Manacher算法在计算最长回文子串长度中的应用

本文首介绍了如何运用Manacher算法在线性时间内找到一个字符串的最长回文子串。