伊犁绢蒿荒漠草地物种光谱特征分析及识别初探

利用便携式地物光谱仪,对天山北坡中段伊犁绢蒿(Seriphidium transiliense)荒漠草地9种植物进行实地光谱测量,对光谱进行一阶微分、平滑、归一化、R_r-R_n光谱特征空间处理,对植物光谱特征分析及识别,并建立判别模型。结果表明,不同植物的原始光谱反射率在特定波段具有一定差异性,一阶微分对于精确提取该类草地植物红边特征参数值有重要作用,平滑处理可以有效去除噪声影响,归一化更有利于发现植物光谱特征,Rr-Rn在区分该类草地植物方面存在困难。选用近红外波段反射率(R_n),红边位置(REP),绿波段反射率(R_g),修改型土壤调节植被指数MSAVI和叶绿素吸收比值指数(CRAI)5项光谱指标建立判别函数,9种植物的判别精度达到90.5%。     

武功山草甸不同海拔对土壤和植物凋落物磷含量的影响

以江西省武功山海拔1 600~1 900m的山地草甸为对象,对不同海拔下,凋落物全磷、土壤全磷、土壤有效磷的空间分布特征以及相互之间的相关性进行分析。结果表明,不同海拔梯度下凋落物全磷、土壤全磷、土壤有效磷分别是0.50~3.53、0.28~1.29、0.19~17.47mg·kg~(-1)。土壤全磷的含量随着海拔的逐渐增加,呈现出"U"型的分布规律,在海拔1 700~1 800m处含量较低。不同物种之间的全磷含量具有明显差异,芒(Miscanthus sinensis)、中华苔草(Carex chinensis)、毛秆野古草(Arundinella hirta)和武功山飘拂草(Fimbristylis wukungshanensis)含量分别是4.21、1.34、1.03、1.28g·kg~(-1),芒的全磷含量显著高于中华苔草、毛秆野古草和武功山飘拂草(P0.05)。相关性分析表明,海拔梯度与土壤全磷之间存在极显著相关性(P0.01),凋落物全磷与土壤有效磷显著正相关,凋落物全磷随着土壤有效磷的增加而增加;凋落物全磷与土壤全磷相关性不显著(P0.05)。说明土壤供磷能力的指标通过土壤有效磷反映,其含量高低主要由凋落物全磷之间的转化方向及分布状况决定,一般认为,凋落物全磷与土壤有效磷的相关性越显著,凋落物全磷的相对有效性就越高。本研究结果有助于进一步了解武功山山地草甸不同海拔梯度对土壤与凋落物磷含量的影响,同时也为山地草甸的植被恢复及可持续经营提供理论依据。     

松嫩草甸草原植物群落物种组成和多样性对刈割和长期放牧的响应

为明晰不同利用方式对松嫩草甸草原不同植物群落物种组成和多样性的影响,本研究通过对松嫩平原西部地带性植被草甸草原在刈割和长期放牧利用下两种典型群落-羊草(Leymus chinensis)群落和野古草(Arundinella hirta)群落的调查与分析,探究了两种利用方式对群落植物物种组成、重要值、生活型、数量特征及多样性等指标的影响。结果表明：刈割和长期放牧下羊草群落物种数为18种和21种,禾本科植物占比最高,分别为33.3%和45.45%;野古草群落物种数分别为39种和32种,禾本科植物占比为23.1%和21.9%。长期放牧增加羊草群落地面芽植物和野古草群落一年生植物占比,降低野古草群落地面芽植物占比。两种利用方式下两个群落饲用价值类型均以优良牧草的种类最多,占比分别为58.76%(刈割羊草群落)、53.33%(长期放牧羊草群落)、46.56%(刈割野古草群落)和46.63%(长期放牧野古草群落)。长期放牧显著降低了两个植物群落的盖度、高度和生物量(P <0.05)。与刈割相比,长期放牧下植物群落的Shannon-Wiener多样性指数和Simpson多样性指数均呈增加趋势。...     

松嫩中部草地退化对植被和土壤特性的影响

研究草地退化演替中植被群落和土壤特性的变化可为退化草地生态恢复提供科学依据。采用野外调查和室内分析的方法,研究了松嫩草地轻度退化[羊草(Leymus chinensis)群落,LCC]、中度退化[野古草(Arundinella hirta)群落,AHC]、重度退化[碱茅(Puccinellia distans)群落,PDC]和极度退化[碱蓬(Suaeda glauca)群落,SGC]植物多样性和土壤理化特性的变化规律。结果表明,草地植物群落组成和物种多样性均随草地退化演替发生了显著变化,羊草群落的物种丰富度、均匀度指数、多样性指数和生物量均高于其他3种植被退化演替阶段。草地退化对土壤理化性状有明显影响。土壤容重表现为羊草群落野古草群落碱茅群落碱蓬群落;羊草群落土壤紧实度小于碱蓬和碱茅群落。羊草群落和野古草群落土壤有机质和全氮含量显著高于碱蓬群落和碱茅群落(P0.05)。土壤全磷含量以羊草群落最高,野古草群落含量最低;羊草群落的土壤速效N和P显著高于碱茅群落和碱蓬群落(P0.05),而碱茅群落土壤速效K含量最高。草地退化演替显著影响植被群落特性,进而改变了土壤理化性状,甚至加剧了该地区土壤盐碱化的发生。     

基于高光谱数据的高寒草地土壤有机碳预测模型研究

土壤退化是草地退化的更深层次指示,运用遥感手段大面积测定土壤有机碳进而评估草地土壤状况有助于对草地退化状态的正确认识。以甘南州高寒草地土壤为研究对象,使用ASD地物光谱仪,在室内条件下对土壤样品进行可见光/近红外光谱测量,分析8种光谱变换形式与土壤有机碳含量的相关性并选取特征波段,利用3种多元回归方法(逐步多元线性回归、主成分回归、偏最小二乘回归),通过验证样本的决定系数(R_v²)、均方根误差(RMSE)和剩余估计偏差(RPD)来评价模型,进而确定高寒草地土壤有机碳的最佳估测模型。结果表明,微分变换方法可以显著提高光谱特征与土壤有机碳含量的相关性,在所有变换形式中以光谱反射率的一阶微分与土壤有机碳含量相关性最好,最大相关系数绝对值为0.865;基于光谱反射率一阶微分变换形式的3种多元回归方法对土壤有机碳均有极好的预测能力,表明对于土壤有机碳的稳定监测来说光谱反射率的一阶微分是非常有效的变换形式;综合考虑基于所有光谱变换形式的3种多元回归方法的预测结果,偏最小二乘回归法具有高的R_v²和RPD,同时具有低的RMSE值,是研究区土壤有机碳估测的最优回归方法;基于光谱反射率对数的一阶微分变换形式所建立的偏最小二乘回归模型具有相对较高的预测集决定系数(R_v²=0.878)、最大剩余估计偏差(RPD=2.946)和最小均方根误差(RMSE=7.520),因此该模型为甘南高寒草地土壤有机碳的最优估测模型,最优模型的RPD大于2.5说明该模型有足够的稳定性可以应用于其他地区土壤有机碳的估测。     

伊犁绢蒿光谱反射率及反演精度对覆盖度变化的响应

为探究覆盖度变化对植物光谱反射率的影响,实现覆盖度和生物量的高精度反演,以伊犁绢蒿(Seriphidium transiliense)为对象,通过室内控制试验获取不同覆盖度下植物光谱反射率,采用最大归一法、一阶微分和二阶微分变换分析其反射率变化,利用归一化植被指数(NDVI)、比值植被指数(RVI)、增强型植被指数(EVI)和土壤调节型植被指数(SAVI)共4种植被指数对覆盖度与生物量进行反演,探讨反演精度对覆盖度变化的响应。结果表明：伊犁绢蒿植物种群的冠层光谱反射率在可见光波段随覆盖度增加逐渐减小,在近红外波段逐渐增加,在680 nm处形成植物特有的红边效应,最大归一化和微分变换突出了植物的光谱特征;光谱反射率差值在可见光波段随覆盖度增加基本稳定;利用植被指数逐级反演覆盖度与生物量时,精度随覆盖度增加整体呈上升趋势;利用土地利用/覆盖变化(LUCC)分覆盖度反演时,提高了覆盖度大于20%的伊犁绢蒿种群的数量特征反演精度,且最佳指数为SAVI。     

祁连县沼泽化高寒草甸亚类优势牧草粗脂肪含量分析

用索式提取法对祁连高寒沼泽湿地中5种优势种牧草进行不同季节粗脂肪含量测定。结果表明:5种优势种牧草粗脂肪含量7月份最高,8月份其次,9月份含量最低;其中,7月份草地早熟禾(Poa pratensis L.)、华扁穗草(Blysmus sinocompressus)、甘肃苔草(Carex kansuensis)、西藏嵩草(Kobresia tibetica)、小钩毛苔草(Carex microglochin)的粗脂肪含量分别为7.32%、5.17%、7.60%、3.61%、6.68%。到9月草地早熟禾粗脂肪含量降低了82.65%,华扁穗草降低了74.08%,甘肃苔草降低了55.66%,西藏嵩草降低了64.27%,小钩毛苔草降低了77.10%。因此,7、8两个月最适合放牧,9月份应适当补充营养。     

祁连山金强河河谷区域高寒灌丛群落海拔梯度影响特征与光谱变化

高寒灌丛是青藏高原生态系统的重要部分,研究高寒灌丛对青藏高原生态系统的研究具有重要意义。光谱遥感技术作为近年来发展迅速的遥感探测技术,可以实现高空间分辨率和高光谱分辨率的地物大面积无损探测。研究高寒灌丛的光谱变换特性有助于光谱遥感技术在青藏高原生态系统研究中的应用。选取青藏高原祁连山东段的甘肃省天祝县金强河河谷区域,根据海拔高度的变化,从2 900 m到3 600 m的阴坡,每100 m设置一个样地,采集每块样地的光谱数据。对采集的光谱数据进行平滑降噪处理后,通过反射率(REF)、吸收率(ABS)及反射率和吸收率的一阶导数(GREF和GABS)变换,分析了灌丛群落和光谱特征随海拔梯度的变化规律。主要结论如下:1)群落多样性的空间格局中,物种多样性随海拔梯度的变化极显著,光谱REF与α多样性随海拔升高均呈现倒"V"字形变化。α多样性最高的海拔梯度与群落光谱REF最大的值均集中在中间海拔区域(3300～3400 m);2)灌丛含水率随海拔梯度的变化呈"N"字形,即灌丛含水率与群落光谱REF随海拔梯度的增加先升高后下降再升高;3)群落高度,生物量和茎叶比对灌丛群落光谱特征的影响较明显。阴坡灌丛群落光谱REF与生物量、茎叶比随海拔梯度的增加呈反"N"形变化;4)千里香杜鹃和头花杜鹃灌丛在整个灌丛群落中占有关键地位,其群落特征的变化对灌丛光谱特征的影响明显。     

苜蓿和无芒雀麦混播草地高光谱遥感估产研究

采用5个新的植被指数(GDVI、GRVDI、GSAVI、GOSAVI、GMSAVI),并与以往遥感估产所用的9个植被指数一起遴选,选出最佳植被指数构建最优模型,用以实现苜蓿和无芒雀麦混播草地快速无破坏性精准测产。结果表明:以近红外光(760nm)和绿光(600nm)光谱变量组合计算的绿色优化土壤调节植被指数(GOSAVI)为自变量构建的二次方程式是混播草地群落最优估产模型,以近红外光(760nm)和绿光(600nm)光谱变量组合计算的绿色土壤调节植被指数(GSAVI)为自变量构建的三次方程式是苜蓿种群最优估产模型,而无芒雀麦种群最优估产模型却是以波长971nm处一阶微分为自变量构建的三次方程式。     

基于DCA-TTLQV的云顶山亚高山草甸群落格局分析

应用除趋势对应分析(DCA)与双项轨迹方差法(TTLQV)相结合的方法,对云顶山亚高山草甸群落格局进行分析。结果表明,在亚高山草甸群落格局分析中,这2种方法的结合可以成功地揭示群落的特征。披针苔草 车前群系、披针苔草 蒲公英群系和嵩草 车前群系的格局差异明显,随着海拔的升高,草甸群落的格局规模增大,群落分布格局不仅受优势种的生物学特性影响,还受环境因子制约,结果很好的揭示了云顶山亚高山草甸群落的结构特征。     

白术、微米白术和白术多糖对断奶仔猪生长性能和肠道形态及微生态区系的影响

试验选用96头平均体重14.82 kg左右的杜×长×大断奶仔猪,随机分成4组,每组3栏,每栏8头(公母各半)。对照组饲喂基础日粮,试验1、2、3组分别添加1%80目白术、0.2%白术多糖和1%微米白术。试验期30 d。结果表明:在生长性能方面,与对照组相比,1%微米白术添加组可显著提高日增重(P0.05)、降低饲料增重比和腹泻率,而且效果优于1%80目白术组和0.2%白术多糖组,在肠道形态和肠道微生态区系方面,与对照组相比,日粮添加1%80目白术、0.2%白术多糖、1%微米白术均可不同程度的提高十二指肠和空肠的绒毛高度,加深十二指肠和空肠的隐窝深度,并且增加肠道微生态区系的多样性,其中以1%微米白术添加组的效果最佳。     

Prevalence and seasonal incidence of nematode parasites and fluke infections of sheep and goats in eastern Ethiopia

Sissay, M.M., Uggla, A. and Waller, P.J., XXXX. Prevalence and seasonal incidence of nematode parasites and fluke infections of sheep and goats in eastern Ethiopia. Tropical Animal Health and Production, XXXX. A 2-year abattoir survey was carried out to determine the prevalence, abundance and seasonal incidence of gastro-intestinal (GI) nematodes and trematodes (flukes) of sheep and goats in the semi-arid zone of eastern Ethiopia. During May 2003 to April 2005, viscera including liver, lungs and GI tracts were collected from 655 sheep and 632 goats slaughtered at 4 abattoirs located in the towns of Haramaya, Harar, Dire Dawa and Jijiga in eastern Ethiopia. All animals were raised in the farming areas located within the community boundaries for each town. Collected materials were transported within 24 h to the parasitology laboratory of Haramaya University for immediate processing. Thirteen species belonging to 9 genera of GI nematodes (Haemonchus contortus, Trichostrongylus axei, T. colubriformis, T. vitrinus, Nematodirus filicollis, N. spathiger, Oesophagostomum columbianum, O. venulosum, Strongyloides papillosus, Bunostomum trigonocephalum, Trichuris ovis, Cooperia curticei and Chabertia ovina), and 4 species belonging to 3 genera of trematodes (Fasciola hepatica, F. gigantica, Paramphistomum {Calicohoron} microbothrium and Dicrocoelium dendriticum) were recorded in both sheep and goats. All animals in this investigation were infected with multiple species to varying degrees. The mean burdens of adult nematodes were generally moderate in both sheep and goats and showed patterns of seasonal abundance that corresponded with the bi-modal annual rainfall pattern, with highest burdens around the middle of the rainy season. In both sheep and goats there were significant differences in the mean worm burdens and abundance of the different nematode species between the four geographic locations, with worm burdens in the Haramaya and Harar areas greater than those observed in the Dire Dawa and Jijiga locations. Similar seasonal variations were also observed in the prevalence of flukes. But there were no significant differences in the prevalence of each fluke species between the four locations. Overall, the results showed that Haemonchus, Trichostrongylus, Nematodirus, Oesophagostomum, Fasciola and Paramphistomum species were the most abundant helminth parasites of sheep and goats in eastern Ethiopia.     

Y‐chromosomal variation of local goat breeds of Turkey close to the domestication centre

Genetic variations in chromosome Y are enabling researchers to identify paternal lineages, which are informative for introgressions and migrations. In this study, the male‐specific region markers, sex‐determining region‐Y (SRY), amelogenin (AMELY) and zinc finger (ZFY) were analysed in seven Turkish native goat breeds, Angora, Kilis, Hair, Honaml?, Norduz, Gürcü and Abaza. A SNP in the ZFY gene defined a new haplotype Y2C. All domestic haplogroups originate from Capra aegagrus, while the finding of Y1A, Y1B, Y2A and Y2C in 32, 4, 126 and 2 Turkish domestic goats, respectively, appears to indicate a predomestic origin of the major haplotypes. The occurrence of four haplotypes in the Hair goat and, in contrast, a frequency of 96% of Y1A in the Kilis breed illustrate that Y‐chromosomal variants have a more breed‐dependent distribution than mitochondrial or autosomal DNA. This probably reflects male founder effects, but a role in adaptation cannot be excluded.     

A brief history of the prevention of infectious diseases by immunisations

Infectious diseases have always been a terrible scourge for humans. The appearance of these plagues, as they were called without distinction, was generally connected to various conditions: asters, climatic changes or religious reasons. The concept of contagious, and then infectious, diseases came slowly. Variolation, i.e. transmission of ‘virulent’ matter to induce a natural disease and the immunity against it, was brought from Constantinople to England by Lady Montague, in 1721. This ‘variolation’ technique was also often performed in veterinary medicine against diseases like sheep-pox or pleuropneumonia. As ‘vaccination’ is the term generally accepted for ‘immunisation’, variolation can be the word designating such a technique. The second period of the history of immunisation began, in 1880, with the studies of Pasteur and his collaborators. A great number of bacterial vaccines were developed: dead, live but attenuated or only parts of pathogens. The viruses were produced in animals, then in eggs and at last, in tissue cultures. Second generation vaccines appeared with genetic engineering: recombinant vaccines, vector vaccines, nucleic acids vaccines, and markers vaccines, among others. These novel technologies can permit the development of new ones and improve the quality of the vaccines already existing.     

Micronutrient Content of Certain Tropical Conventional and Unconventional Feed Resources of Southern India

The ash, silica and certain important micronutrients were estimated in conventional and unconventional feed and fodder resources available in Southern India. Commonly used dry roughages, such as paddy straw, ragi straw, maize kadbi/stalk, jowar kadbi/stalk, bajra stalk and wheat straw, were high in ash (9.9% +/- 0.77%) and silica (6.4% +/- 0.65%) and low in most of the other micronutrients, except iron, with paddy straw containing most silica (>9%). Cultivated non-leguminous (maize, jowar) and improved green crops (hybrid napier, guinea, green panic, NB-21, CO-1) were also high in ash (10.5% +/- 0.60% and 12.5% +/- 0.51%) but were moderate sources of P, Mg and Cu and good sources of Zn (98 +/- 13.8 ppm and 55 +/- 6.7 ppm). Leguminous green fodders (stylosanthus, lucerne, cow pea, soyabean) were excellent sources of Ca (1.9% +/- 0.16%), Mg (0.40% +/- 0.05%), Cu (30 +/- 5.2 ppm), Zn (121 +/- 14.7 ppm) and Fe (1234 +/- 166 ppm) and moderate sources of P. Mixed local grasses and weeds were high in silica (6.9% +/- 1.00%) but were good sources of Cu, Zn and Fe. Cereal grains (maize, wheat, rice, ragi) were low in ash (2.9% +/- 0.33%) and were relatively poor sources of Ca (0.22% +/- 0.03%), Mg (0.19% +/- 0.03%) and Cu (13 +/- 3.1 ppm). Pulses were low to medium sources of most minerals and good sources of Fe (1230 +/- 293 ppm). Oil seed cake/extractions (groundnut cake, cotton seed cake, soyabean meal, sunflower cake, safflower cake) and cereal by-products (rice polish, rice bran, wheat bran) were excellent sources of P (1.1% +/- 0.47% and 2.3% +/- 0.19%) and good sources of Zn (65 +/- 3.9 ppm and 66 +/- 10.7 ppm) and Fe (938 +/- 130 ppm and 662 +/- 126 ppm). Among the unconventional feeds screened, orange peel, sunflower heads, meat meal, rubber seed cake, spirulina algae and sea weeds contained plentiful Ca, Zn and Fe: tree leaves/top feeds (mulberry, erythrina, glyricidia, banana, subabul, groundnut haulms) were excellent sources of Ca (1.5% +/- 0.13%), Zn (120 +/- 22.9 ppm) and Fe (1033 +/- 133 ppm) but relatively poor sources of P. Soyabean husk, cocoa seed husk, rubber seed cake and meat meal were moderate to good sources of P (1.0% and 0.33%). The high Zn and Fe values of most feeds/fodders were probably due to soil contamination. This account of the micronutrient content of feed/fodder resources should help in strategic supplementation intended to alleviate local deficiencies.     

Feline intracranial neoplasia: retrospective review of 160 cases (1985-2001)

The purpose of this study was to determine the frequency of different tumor types within a large cohort of cats with intracranial neoplasia and to attempt to correlate signalment, tumor size and location, and survival time for each tumor. Medical records of 160 cats with confirmed intracranial neoplasia evaluated between 1985 and 2001 were reviewed. Parameters evaluated included age, sex, breed, FeLV/FIV status, clinical signs, duration of signs, number of tumors, tumor location(s), imaging results, treatment, survival times, and histopathologic diagnosis. Most of the cats were older (11.3 +/- 3.8 years). Primary tumors accounted for 70.6% of cases. Metastasis and direct extension of secondary tumors accounted for only 5.6 and 3.8% of cases, respectively. Twelve cats (7.5%) had 2 or more discrete tumors of the same type, whereas 16 cats (10.0%) had 2 different types of intracranial tumors. The most common tumor types were meningioma (n = 93, 58.1%), lymphoma (n = 23, 14.4%), pituitary tumors (n = 14, 8.8%), and gliomas (n = 12, 7.5%). The most common neurological signs were altered consciousness (n = 42, 26.2%), circling (n = 36, 22.5%), and seizures (n = 36, 22.5%). Cats without specific neurological signs were common (n = 34, 21.2%). The tumor was considered an incidental finding in 30 (18.8%) cats. In addition to expected relationships (eg, meninges and meningioma, pituitary and pituitary tumors), we found that lesion location was predictive of tumor type with diffuse cerebral or brainstem involvement predictive of lymphoma and third ventricle involvement predictive of meningioma.     

Bovine Mastitis in Selected Areas of Southern Ethiopia

A study on bovine mastitis, designed to determine the causal agents, prevalence of infection and impact of risk factors in three cattle breeds, was conducted in selected areas of southern Ethiopia. A total of 307 lactating and non-lactating cows, of which 162 were indigenous Zebu, 85 Jersey and 60 Holstein-Friesian, were examined by clinical examination and the California mastitis (CMT) test. Of these, 40.4% were positive by CMT and bacteriology for clinical or subclinical mastitis, with prevalence rates of 37.1% and 62.9%, respectively. Out of 1133 quarters examined, 212 (18.7%) were found to be infected, 83 (39.2%) clinically and 129 (60.8%) subclinically. The prevalence of mastitis was significantly higher in Holstein-Friesian than in indigenous Zebu, in non-lactating cows than in lactating cows, in the early lactation stage than in the mid-lactation stage, in cows with lesions and/or tick infestation on skin of udder and/or teats than in cows without this factor, and in the wet season than in the dry season. Mastitis increased with parity number (R = 0.9). Of 248 CMT and clinically positive udder quarter samples analysed microbiologically, 212 were culturally positive for known mastitis pathogens and 36 were negative. Of the 199 positive samples, Staphylococcus accounted for 39.2%, Streptococcus for 23.6%, coliforms for 14.1%, Micrococcus and Bacillus species for 8.0% each and Actinomyces or Arcanobacterium (Corynebacterium) for 7.0%. It was concluded that there was a high prevalence of clinical and subclinical mastitis, mainly caused by Staphylococcus aureus, Streptococcus agalactiae and Escherichia coli, in this study area.     

不同日粮氮水平对山羊氮代谢和微生物蛋白质合成的影响

本试验旨在探讨不同日粮氮水平对内蒙古白绒山羊氮代谢和微生物蛋白质合成(MCP)的影响,以达到提高氮利用率,减少环境污染和饲料资源的浪费。试验选用9只体况良好、体重为(43.83±2.95)kg,装有瘤胃瘘管的内蒙古白绒山羊,按体重随机分为3组,每组3只。日粮分为低氮7.5%、中氮10.5%、高氮13.5%3个氮水平。试验使用常规试验方法分析测定粪尿血液等生化指标,通过全收尿法和尿嘌呤衍生物法估测MCP。结果表明:随着氮水平增加,氨氮(NH3-N)浓度、氮摄入量、尿氮排出、尿中尿素氮(UUN)和MCP显著增加(P0.05),干物质采食量(DMI)显著降低(P0.01);沉积氮占总摄入氮的比例在中氮组最高,MCP在高氮组最高。综上所述,适当降低日粮氮水平可减少粪尿氮排放,提高反刍动物氮利用率。     

Microsporidial keratopathy in two dogs

A microsporidial keratopathy is described in two dogs. Both dogs presented with a unilateral stromal keratopathy characterized by multifocal coalescing opacities, and the diagnosis was made on histopathologic examination of keratectomy specimens. Transmission electron microscopy (TEM) on formalin‐fixed, paraffin‐embedded corneal tissue was performed in one dog, and the morphologic features were consistent with Nosema species infection. Both dogs were initially diagnosed and treated by superficial keratectomy. One dog received additional antifungal medication and underwent a penetrating keratoplasty following local recurrence two years later. No other systemic lesions attributable to the microsporidial infection were identified clinically. The clinical and diagnostic pathology findings, treatment, and follow‐up are discussed.