Effects of freezing intensity on soil solution nitrogen and microbial biomass nitrogen in an alpine grassland ecosystem on the Tibetan Plateau,China

The change of freeze-thaw pattern of the Tibetan Plateau under climate warming is bound to have a profound impact on the soil process of alpine grassland ecosystem;however,the research on the impact of the freeze-thaw action on nitrogen processes of the alpine grassland ecosystem on the Tibetan Plateau has not yet attracted much attention.In this study,the impact of the freezing strength on the soil nitrogen components of alpine grassland on the Tibetan Plateau was studied through laboratory freeze-thaw simulation experiments.The 0–10 cm topsoil was collected from the alpine marsh meadow and alpine meadow in the permafrost region of Beilu River.In the experiment,the soil samples were cultivated at –10°C,–7°C,–5°C,–3°C and –1°C,respectively for three days and then thawed at 2°C for one day.The results showed that after the freeze-thaw process,the soil microbial biomass nitrogen significantly decreased while the dissolved organic nitrogen and inorganic nitrogen significantly increased.When the freezing temperature was below –7°C,there was no significant difference between the content of nitrogen components,which implied a change of each nitrogen component might have a response threshold toward the freezing temperature.As the freeze-thaw process can lead to the risk of nitrogen loss in the alpine grassland ecosystem,more attention should be paid to the response of the soil nitrogen cycle of alpine grasslands on the Tibetan Plateau to the freeze-thaw process.     

Interactive effects of soil temperature and moisture on soil N mineralization in a Stipa krylovii grassland in Inner Mongolia,China

Determining soil N mineralization response to soil temperature and moisture changes is challenging in the field due to complicated effects from other factors. In the laboratory, N mineralization is highly dependent on temperature, moisture and sample size. In this study, a laboratory incubation experiment was carefully designed and conducted under controlled conditions to examine the effects of soil temperature and moisture on soil N mineralization using soil samples obtained from the Stipa krylovii grassland in Inner Mongolia, China. Five temperature(i.e. 9°C, 14°C, 22°C, 30°C and 40°C) and five moisture levels(i.e. 20%, 40%, 60%, 80% and 100% WHC, where WHC is the soil water holding capacity) were included in a full-factorial design. During the 71-day incubation period, microbial biomass carbon(MBC), ammonium nitrogen(NH4 +-N) and nitrate nitrogen(NO3--N) were measured approximately every 18 days; soil basal respiration for qCO2 index was measured once every 2 days(once a week near the end of the incubation period). The results showed that the mineral N production and net N mineralization rates were positively correlated with temperature; the strongest correlation was observed for temperatures between 30°C and 40°C. The relationships between moisture levels and both the mineral N production and net N mineralization rates were quadratic. The interaction between soil temperature and moisture was significant on N mineralization, i.e. increasing temperatures(moisture) enhanced the sensitivity of N mineralization to moisture(temperature). Our results also showed a positive correlation between the net nitrification rate and temperature, while the correlation between the NH4 +-N content and temperature was insignificant. The net nitrification rate was negatively correlated with high NH4 +-N contents at 80%–100% WHC, suggesting an active denitrification in moist conditions. Moreover, qCO2 index was positively correlated with temperature, especially at 80% WHC. With a low net nitrification rate and high soil basal respiration rate, it was likely that the denitrification concealed the microbial gross mineralization activity; therefore, active soil N mineralization occurred in 60%–80% WHC conditions.     

Impact of enclosure management on soil properties and microbial biomass in a restored semi-arid rangeland,Kenya

Rangeland degradation is a serious problem throughout sub-Saharan Africa and its restoration is a challenge for the management of arid and semi-arid areas. In Lake Baringo Basin of Kenya, communities and individual farmers are restoring indigenous vegetation inside enclosures in an effort to combat severe land degradation and address their livelihood problems. This study evaluated the impact of enclosure management on soil properties and microbial biomass, being key indicators of soil ecosystem health. Six reseeded communal enclosures using soil embankments as water-harvesting structures and strictly regulated access were selected, varying in age from 13 to 23 years. In six private enclosures, ranging from 3 to 17 years in age, individual farmers emulated the communal enclosure strategy and restored areas for their exclusive use. Significant decreases in bulk density, and increases in the soil organic carbon, total nitrogen and microbial biomass contents and stocks were found in the enclosures as compared with the degraded open rangeland. In the private enclosures, the impact of rehabilitation on the soil quality was variable, and soil quality was in general lower than that obtained under communal management. The significant increase of absolute stocks of carbon, nitrogen and microbial biomass compared to the degraded open rangeland indicates the potential for the restoration of soil quality through range rehabilitation. Over-sowing with indigenous legume fodder species could improve total nitrogen content in the soil and nutritional value of the pastures as well.     

Soil bacterial characteristics between surface and subsurface soils along a precipitation gradient in the Alxa Desert,China

Bacteria in desert soil have unique phylogeny and important ecological functions, and theirresponses to changes in precipitation need further attention. However, relevant studies have mainlyfocused on the surface soil, and studies on the responses of bacteria at different soil depths to variationsin precipitation are rare. Thus, we used 16S rDNA high-throughput sequencing to investigate the changesin soil bacterial distribution along a mean annual precipitation gradient (50–150 mm) in the Alxa Desert,China, and compared the variation characteristics in the surface soil layer (0–10 cm) and subsurface soillayer (10–20 cm). Results showed that soil bacterial communities significantly changed along theprecipitation gradient in both soil layers. However, the subsurface soil layer could support bacterialcommunities with higher diversity and closer internal relationships but more internal competition than thesurface soil layer. Additionally, compared with the surface soil layer, variations in diversity andco-occurrence patterns in the subsurface soil layer were more in line with the changes in the mean annualprecipitation, while bacterial community structure was less variable in the subsurface soil layer. Comparedwith the mean annual precipitation, soil moisture had little influence on the structure and diversity of soilbacterial community but had a high correlation with intercommunity connectivity. Therefore, soilmoisture might play a complex role in mediating environmental conditions and soil bacterial communitycharacteristics. Due to the different responses of surface and subsurface soil bacteria to the changes inprecipitation, it is necessary to distinguish different soil layers when predicting the trends in desert soilbacterial conditions associated with precipitation, and prediction of subsurface soil bacteria may be moreaccurate.     

Effects of artificially cultivated biological soil crusts on soil nutrients and biological activities in the Loess Plateau

Biological soil crusts （BSCs） play an important role in the early succession of vegetation restoration in the Loess Plateau, China. To evaluate the effects of artificially cultivated BSCs on the soil surface micro-envir- onment, we obtained natural moss crusts and moss-lichen crusts from the Loess Plateau of Shaanxi province, and subsequently inoculated and cultivated on horizontal and sloping surfaces of loess soil in a greenhouse. The chemical and biological properties of the subsoil under cultivated BSCs were determined after 10 weeks of cul- tivation. The results indicated that BSCs coverage was more than 65% after 10 weeks of cultivation. Moss crust coverage reached 40% after 5 weeks of cultivation. Compared with the control, soil organic matter and available nitrogen contents in moss crust with the horizontal treatments increased by 100.87% and 48.23%, respectively; increased by 67.56% and 52.17% with the sloping treatments, respectively; they also increased in moss-lichen crust with horizontal and sloping treatments, but there was no significant difference. Available phosphorus in cultivated BSCs was reduced, soil pH was lower and cationic exchange capacity was higher in cultivated BSCs than in the control. Alkaline phosphatase, urease and invertase activities were increased in artificially cultivated BSCs, and alkaline phosphatase activity in all cultivated BSCs was obviously higher than that in the control. Numbers of soil bacteria, fungi and actinomycetes were increased in the formation process of cultivated BSCs. These results indicate that BSCs could be formed rapidly in short-term cultivation and improve the mi- cro-environment of soil surface, which provides a scientific reference for vegetation restoration and ecological reconstruction in the Loess Plateau. China.     

Allometric response of perennial Pennisetum centrasiaticum Tzvel to nutrient and water limitation in the Horqin Sand Land of China

Optimal partitioning theory(OPT)suggests that plants should allocate relatively more biomass to the organs that acquire the most limited resources.The assumption of this theory is that plants trade off the biomass allocation between leaves,stems and roots.However,variations in biomass allocation among plant parts can also occur as a plant grows in size.As an alternative approach,allometric biomass partitioning theory(APT)asserts that plants should trade off their biomass between roots,stems and leaves.This approach can minimize bias when comparing biomass allocation patterns by accounting for plant size in the analysis.We analyzed the biomass allocation strategy of perennial Pennisetum centrasiaticum Tzvel in the Horqin Sand Land of northern China by treating samples with different availabilities of soil nutrients and water,adding snow in winter and water in summer.We hypothesized that P.centrasiaticum alters its pattern of biomass allocation strategy in response to different levels of soil water content and soil nitrogen content.We used standardized major axis(SMA)to analyze the allometric relationship(slope)and intercept between biomass traits(root,stem,leaf and total biomass)of nitrogen/water treatments.Taking plant size into consideration,no allometric relationships between different organs were significantly affected by differing soil water and soil nitrogen levels,while the biomass allocation strategy of P.centrasiaticum was affected by soil water levels,but not by soil nitrogen levels.The plasticity of roots,leaves and root/shoot ratios was‘true’in response to fluctuations in soil water content,but the plasticity of stems was consistent for trade-offs between the effects of water and plant size.Plants allocated relatively more biomass to roots and less to leaves when snow was added in winter.A similar trend was observed when water was added in summer.The plasticity of roots,stems and leaves was a function of plant size,and remained unchanged in response to different soil nitrogen levels.     

Responses of plant community to the linkages in plant-soil C:N:P stoichiometry during secondary succession of abandoned farmlands,China

Succession is one of the central themes of ecology;however,the relationship between aboveground plant communities and underground soils during secondary succession remains unclear.In this study,we investigated the composition of plant community,plant-soil C:N:P stoichiometry and their relationships during secondary succession after the abandonment of farmlands for 0,10,20,30,40 and 50 a in China,2016.Results showed that the composition of plant communities was most diverse in the farmlands after secondary succession for 20 and 50 a.Soil organic carbon and total nitrogen contents slightly decreased after secondary succession for 30 a,but both were significantly higher than those of control farmland(31.21%-139.10%and 24.24%-121.21%,respectively).Moreover,C:N ratios of soil and microbe greatly contributed to the changes in plant community composition during secondary succession of abandoned farmlands,explaining 35.70%of the total variation.Particularly,soil C:N ratio was significantly and positively related with the Shannon-Wiener index.This study provides the evidence of synchronous evolution between plant community and soil during secondary succession and C:N ratio is an important linkage between them.     

Quantifying the impacts of soil water stress on the winter wheat growth in an arid region, Xinjiang

Wheat growth in response to soil water deficit play an important role in yield stability. A field experiment was conducted for winter wheat (Triticum aestivum L.) during the period of 2002-2005 to evaluate the effects of limited irrigation on winter wheat growth. 80%, 70%, 60%, 50% and 40% of field capacity was applied at different stages of crop growth. Photosynthetic characteristics of winter wheat, such as photosynthesis rate, transpiration rate, stomatal conductance, photosynthetically active radiation, and soil water content, root and shoot dry mass accumulation were measured, and the root water uptake and water balance in different layer were calculated. Based on the theory of unsaturated dynamic, a one-dimensional numerical model was developed to simulate the effect of soil water movement on winter wheat growth using Hydrus-1 D. The soil water content of stratified soil in the experimental plot was calculated under deficit irrigation. The results showed that, in different growing periods, evapotranspiration, grain yield, biomass, root water up- take, water use efficiency, and photosynthetic characteristics depended on the controlled ranges of soil water content. Grain yield response to irrigation varied considerably due to differences in soil moisture contents and irrigation scheduling between seasons. Evapotranspiration was largest in the high soil moisture treatment, and so was the biomass, but this treatment did not produce the highest grain yield and root water uptake was relatively low. Maximum depth of root water uptake is from the upper 80 cm in soil profile in jointing stage and dropped rapidly upper 40 cm after heading stage, and the velocity of root water uptake in latter stage was less than that in middle stage. The effect of limited irrigation treatment on photosynthesis was complex owing to microclimate. But root water uptake increased linearly with harvest yield and improvement in the latter gave better root water uptake under limited irrigation conditions. Appropriately controlled soil wate     

Effects of aeolian processes on nutrient loss from surface soils and their significance for sandy desertification in Mu Us Desert,China: a wind tunnel approach

Mu Us Desert, a region with high aeolian activity, is at extremely high risk of sandy desertification. Using surface soil samples collected from Mu Us Desert of northern China, we evaluated the effects of aeolian processes on nutrient loss from surface soils by employing wind tunnel experiments. The experiments were conducted using free-stream wind velocities of 14, 16, 18 and 22 m/s. Our results showed that the fine particles(50 μm in diameter; 12.28% of all transported materials) carrying large nutrient loadings were exported outside the study area by aeolian processes. After the erodible fine particles were transported away from the soil surfaces at low wind velocity(i.e. 14 m/s), the following relatively high wind velocity(i.e. 22 m/s) did not have any significant effect on nutrient export, because the coefficients of variation for soil organic matter, total phosphorus, total nitrogen and available potassium were usually 5%. Our experimental results confirmed that aeolian processes result in a large amount of nutrient export, and consequently increase the risk of sandy desertification in arid and semi-arid ecosystems.     

An analytical model for estimating soil temperature profiles on the Qinghai-Tibet Plateau of China

Soil temperature is a key variable in the control of underground hydro-thermal processes. To estimate soil temperature more accurately, this study proposed a solution method of the heat conduction equation of soil temperature(improved heat conduction model) by applying boundary conditions that incorporate the annual and diurnal variations of soil surface temperature and the temporal variation of daily temperature amplitude, as well as the temperature difference between two soil layers in the Tanggula observation site of the Qinghai-Tibet Plateau of China. We employed both the improved heat conduction model and the classical heat conduction model to fit soil temperature by using the 5 cm soil layer as the upper boundary for soil depth. The results indicated that the daily soil temperature amplitude can be better described by the sinusoidal function in the improved model, which then yielded more accurate soil temperature simulating effect at the depth of 5 cm. The simulated soil temperature values generated by the improved model and classical heat conduction model were then compared to the observed soil temperature values at different soil depths. Statistical analyses of the root mean square error(RMSE), the normalized standard error(NSEE) and the bias demonstrated that the improved model showed higher accuracy, and the average values of RMSE, bias and NSEE at the soil depth of 10–105 cm were 1.41°C, 1.15°C and 22.40%, respectively. These results indicated that the improved heat conduction model can better estimate soil temperature profiles compared to the traditional model.     

石油烃降解混合菌修复稠油污染土壤的影响因素

稠油在开采、贮运、炼制加工及使用过程中,不可避免的污染土壤环境,对人体健康和环境造成严重影响,因此有必要采取有效措施对稠油污染土壤进行修复。利用筛选获得的石油烃降解混合菌KL9-1,对稠油污染土壤进行修复研究,考察修复过程中的影响因素。结果表明：pH、接种量、土壤中N/P、表面活性剂用量、翻耕频率、浇水频率和膨松剂种类等因素对污染土壤修复有明显的影响。 在每千克稠油污染土壤体系中,控制初始pH为8.0,接种量为70.0 mL,N∶P为3∶1,表面活性剂用量为4.0 g,每2 d浇水1次,4 d翻耕1次,稻壳作为膨松剂,在此条件下经过70 d的生物修复,石油烃降解率最高可达54.07%。     

烟嘧磺隆降解菌株DT-4的降解特性及对高粱药害的缓解作用

土壤中烟嘧磺隆残留可对后茬作物产生不同程度药害。为解决玉米地烟嘧磺隆残留对后茬作物高粱药害问题,使用前期筛选出的烟嘧磺隆高效降解菌株绿木霉Trichoderma virens DT-4,通过高效液相色谱-质谱法检测其降解率,利用室内盆栽生测结合田间小区试验方法,确定降解菌株对土壤中烟嘧磺隆降解效果,降低烟嘧磺隆残留对高粱的药害。结果显示,烟嘧磺隆残留浓度在0.0025～0.1 ug/mL范围内,标准曲线线性良好,符合农残分析要求;PDB液体培养基中烟嘧磺隆在DT-4降解菌处理100 h的降解率高达93.00%;菌株最佳降解条件为培养温度35 ℃、pH 5、最适接种量5%、烟嘧磺隆初始质量浓度200 mg/L。盆栽试验土壤中烟嘧磺隆残留浓度为0.075 mg/kg时,加入降解菌DT-4后能够明显缓解高粱的株高、根长、鲜重、叶绿素含量、根系活力和净光合速率等生长发育指标,对高粱生长有明显的促进作用。田间小区试验中,不同剂型降解菌剂处理,高粱抽穗期效果最为显著,降解菌能够将低于60 g a.i./hm²烟嘧磺隆污染土壤的高粱修复至无明显药害水平,且降解菌剂应用效果为粉剂＞菌液。本研究表明高效降解菌株绿木霉DT-4可有效缓解烟嘧磺隆残留对后茬作物高粱的毒害作用,为玉米高粱轮作体系下烟嘧磺隆残留污染土壤的微生物修复提供科学参考。     

塔里木绿洲种植制度对棉田土壤养分性状的影响

为了研究塔里木绿洲种植制度对棉田土壤养分特性的影响,以棉田轮作方式(水稻→棉花、水稻→冬麦→棉花、水稻→冬麦-绿肥→棉花)和连作年限(3、5、8、10、15 a)为试验因素,对0~100 cm土层土壤进行了调查与试验。结果表明:短期轮作对棉田土壤养分含量无影响,棉花连作年限是不同层次土壤养分含量变化的主要影响因素,连作3、8、10、15 a土壤0~20 cm速效氮平均为40.60、48.75、51.96、44.35 mg/kg;速效磷平均为10.16、26.72、27.00、23.37 mg/kg;速效钾平均为184.70、142.60、130.20、105.56 mg/kg;有机质含量分别为10.43 g/kg、12.10g/kg、12.93 g/kg、13.56 mg/kg;无论轮作类型、连作年限长短,从垂直分布上,连作15 a、3 a土壤速效氮F值分别为79.01、299.45;速效磷F值分别为88.99、17.54;有机质F值分别为77.27和171.6。表明长期连作降低了上下层土壤速效氮和有机质含量差异而增加了上下层土壤速效磷含量差异;有机质含量随着土层深度的增加而降低;速效钾含量有随着土层深度的增加而增加的趋势。棉花长期连作有利于增加各层次土壤速效氮、速效磷和有机质含量,同时降低深层土壤速效钾的含量;连作8~10 a耕作层土壤速效氮、有效磷含量达到最大值分别为52.35 mg/kg和27.45 mg/kg。     

土壤中降解涕灭威菌株的分离鉴定及降解特性

为探明土壤微生物对涕灭威的降解能力,用富集培养法分离驯化土壤中涕灭威的优势降解菌,初步筛选出了对涕灭威具有较高降解能力的菌株TB26和100-8。经过生理生化鉴定和16S rDNA序列同源性分析,将菌株TB26初步鉴定为克雷伯杆菌属(Klebsiella sp.),菌株100-8初步鉴定为枯草芽孢杆菌属(Bacillus sp.)。TB26和100-8生长的最适碳源分别为麦芽糖、D-果糖,最适氮源分别为蛋白胨、脲。基础无机盐培养基和缺氮培养基对两种菌的生长情况及降解率的影响不同,外加氮源能够提高100-8的降解率,而缺氮培养基中TB26的降解率较高。     

水肥供应对番茄生长及产量形成的影响

以"金鹏1号"番茄为试验材料,通过盆栽试验,对处于不同灌水量和氮、磷、钾肥用量条件下的番茄生长及产量进行分析。在此试验条件下,各因素对叶片数的因子贡献率的主次关系为:施氮量=施磷量灌水量施钾量;对叶果比的影响表现为:灌水量施氮量施磷量施钾量;坐果率表现为:灌水量施氮量施磷量施钾量;60%~83.78%的灌水量有利于降低叶果比进而促进产量的增加;氮肥、磷肥用量达到0.96 g·kg~(-1)、P_2O_50.528 g·kg~(-1)土时会导致叶片数和叶果比增加,坐果率和产量下降;随着灌水量、施氮量、施钾量的增加番茄产量呈先增后减的趋势,在灌水量为83.78%W,施氮量为0.77N g·kg~(-1)土,施磷量为P_2O_50.421 g·kg~(-1)土,施钾量为K2O 0.670g·kg~(-1)土时产量达到最大值为467.5 g·株~(-1)。     

环境材料对不同水肥条件下玉米氮磷利用效率的影响

为了探究不同水肥条件下环境材料对作物生长和氮磷利用效率的效应,通过盆栽玉米种植实验采用土壤持水量(A)、氮磷肥(B)、环境材料(C)三因素三水平正交实验设计,研究了高分子保水剂、沸石和腐植酸等环境材料组合对玉米生长和氮磷肥利用效率影响,筛选出作物增产和氮磷肥同步增效的三因素优化组合,并通过土壤肥力和酶活性分析揭示相关效应机理。结果表明,三因素对各项指标影响的主次顺序不同,A和B对玉米生长和氮磷肥利用效率的影响大于C,但C能够促进玉米生长和氮磷肥利用效率的提高,促进土壤碱解氮、有效磷含量的提高以及脲酶、磷酸酶活性的增加;土壤持水量65%~70%,每公斤土施肥1.5 g N、1.13 g P_2O_5,环境材料4.5 g的组合和土壤持水量85%~90%,每公斤土施肥1.5 g N、1.13 g P_2O_5,环境材料9 g的组合,作为提高玉米产量和氮磷肥利用效率的优化组合,在实践中可根据水分情况进行选择。     

仁用杏园不同土层土壤养分含量的测定

在陕北黄土丘陵沟壑区，对仁用杏园的土壤有机质、全氮、全磷、速效氮、速效磷、速效钾进行了测定。0～500cm土层的土壤有机质为3．368g／kg，全氮为0．268g／kg，全磷为0．536g／kg，速效氮为13．644mg／kg。速效磷为2．202mg／kg，速效钾为50．406mg／kg。陕北黄土丘陵沟壑区仁用杏园无论是浅层、根系集中分布层还是源层土壤，土壤养分含量均较低，满足不了仁用杏的正常生长与开花结实。     

秸秆还田对半干旱区农田土壤养分含量及玉米光合作用的影响

为了提升和保育辽西半干旱区农田土壤质量,采用盆栽试验的方式探讨了不同处理方式、不同用量玉米秸秆还田对土壤养分含量的影响。结果表明,秸秆还田后,其土壤含水量比对照组提高了3%~7%;有机质含量比对照组提高幅度为1.9%~19%;全氮含量比对照组提高幅度为0.7%~14.4%;速效磷含量比对照组提高了2.51~14.75 mg/kg;速效钾含量比对照组提高了7.33~188.07 mg/kg;碱解氮含量比对照组提高了8.46mg/kg;净光合速率比对照组提高了3~11.03 CO2μmol/(m2.s);秸秆还田可以缓解土壤碱化。     

基于低污染的宁夏引黄灌区13种种植模式氮磷平衡分析

针对宁夏引黄灌区农业生产中大量施用化肥对环境及农业退水污染严重的实际,本试验以宁夏引黄灌区现有小麦/玉米为对照,通过选择冬小麦复种水稻及组配麦后复种(蔬菜、青贮玉米、油葵等)等13种种植模式,研究不同模式在作物不同生长时期内土壤氮磷运移规律以及综合效益,选择利于农业清洁生产的种植模式。结果表明:相对于单作,间套作由于增加了田面作物的覆盖时间,对降低土壤氮磷残留量效果明显;以土壤氮磷为主要衡量指标,结合经济效益,筛选出了适合于该灌区大面积种植,经济效益较高的四种低污染模式为:冬小麦复种水稻、春小麦复种芹菜、冬小麦复种青贮、冬小麦复种油葵,四种模式中尤以冬小麦复种水稻效益最明显,土壤中氮流失总量比对照低56.2%,磷流失总量比对照低28.6%。     

粪产碱杆菌ZWS11菌株对烟嘧磺隆的酶促降解特性

为明确粪产碱杆菌Alcaligenes faecalis ZWS11菌株对烟嘧磺隆的酶促降解特性，采用丙酮沉淀、超声波破碎和高速离心等方法，提取制备了ZWS11菌株的胞外和胞内粗酶液以及菌体碎片悬浮液，并分别测定了其酶活力。结果表明:当V（菌体发酵液）:V（丙酮）=1:3时提取到的胞外粗酶液具有较高的酶活力；胞外粗酶液、胞内粗酶液和菌体碎片悬浮液对24.9 μmol/L烟嘧磺隆的平均降解率分别为87.4%、16.9%和17.4%，胞外粗酶液的降解能力与胞内粗酶液或菌体碎片悬浮液相比差异显著（P<0.05），由此确定对烟嘧磺隆起降解作用的酶属于胞外酶。最适降解条件研究表明:该降解酶的酶促反应适宜温度为35℃，较适pH值为6.0，反应时间为30 min，在此条件下其对烟嘧磺隆的降解率在80%以上。此外，该降解酶在35～70℃、pH值4.0～9.0范围内均能够保持较高的酶活力，对烟嘧磺隆的降解率均在60%以上，表明该降解酶具有较好的热稳定性和酸碱稳定性。加入不同浓度十二烷基硫酸钠（SDS）和苯甲基磺酰氟（PMSF），对该降解酶的活力表现出了不同程度的抑制作用。研究结果可为烟嘧磺隆降解酶制剂的规模化生产及被烟嘧磺隆污染土壤的生物修复提供科学依据。