Comparative effects of temperature and humidity on the activity of three potential antagonists of rose powdery mildew

Three reported antagonists of cucumber powdery mildew,Stephanoascus flocculosus, Stephanoascus rugulosus, andTilletiopsis washingtonensis, were tested and compared under different environmental conditions for their potential for controlling rose powdery mildew, caused bySphaerotheca pannosa var.rosae. Under controlled conditions in vitro, all three fungi induced a rapid collapse of conidia, conidiophores and hyphae ofS. pannosa var.rosae on detached leaflets of miniature roses within 48 h following their application, as observed under a SEM. Both temperature and relative humidity (r.h.) affected the activity of the antagonists differently. The colonization of powdery mildew was maximal at 26 °C, especially forSt. rugulosus andT. washingtonensis. Maximal colonization was achieved at the highest r.h. tested (90%) for all three antagonists but onlySt. flocculosus maintained a colonization of 80% or better under lower r.h. These observations stress the importance of considering environmental conditions when assessing the activity of antagonistic microorganisms.     

Using environmental factors to predict Rhipicephalus sanguineus s.l. (Acari: Ixodidae) mortality

Background

Rhipicephalus sanguineus s.l. (Latreille, 1806) can establish indoor populations, which increases the risk of pathogen transmission to humans and companion dogs. Rhipicephalus sanguineus s.l. ticks spend most of their life cycle off the host, which subjects developmental timescale to abiotic factors. Previous studies showed that both temperature and relative humidity (RH) influenced Rhipicephalus sanguineus s.l. survival time across all life stages. However, quantified relationships between environmental factors and Rhipicephalus sanguineus s.l. mortality is not currently available. Here, three Rhipicephalus sanguineus s.l. strains were evaluated for mortality under 20 combinations of five temperatures and four RHs. The data obtained were analyzed to quantify the relationship between environmental factors and Rhipicephalus sanguineus s.l. survival.

Results

Mortality probabilities did not show a consistent pattern between the three tick strains. Temperature, RH, and their interaction influenced Rhipicephalus sanguineus s.l. mortality probabilities across all life stages, with mortality probability generally increasing with temperature but decreasing with RH. With 50% and lower RH, larvae cannot survive for more than 1 week. However, mortality probabilities in all strains and stages were more sensitive to temperature than to RH.

Conclusion

This study identified the predictive relationship between environmental factors and Rhipicephalus sanguineus s.l. survival, which enables estimations of tick survival time under varied residential situations, allows parameterization of population models, and provides guidance for pest control professionals to develop efficient management strategies. © 2023 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.     

State of the art regarding carrot fly control in practice and possibilities in the future for Western and Northern Europe1

The carrot fly (Psila rosae) is a major pest of umbelliferous crops, particularly in carrot production. This pest is present in temperate regions of Northern and Western Europe and also in the warm and dry climate of Central and Southern Europe. Currently, control strategies are limited and there may be a bottleneck of pest control solutions in the future. In practice, growers in the Netherlands mainly rely on a commercial, supervised control system which monitors the carrot fly population pressure. Possibilities to control the carrot fly after exceeding an economical threshold are reduced due to a lack of efficient chemical insecticides. Another option is the use of seed treatment to protect the carrots against the larvae developed from the first flight of the carrot flies for 12–14 weeks after sowing. The state of the art of the carrot fly pest and control possibilities are hereby discussed. Bottlenecks in control of carrot flies are highlighted and described.     

基于MaxEnt模型的突颊侧琵甲新疆潜在地理分布预测和主要影响因子分析

为有效预警和防控突颊侧琵甲Prosodes(Lioprosodes)dilaticollis Motschulsky发生,基于该物种分布数据、1950―2000年环境下生物气候数据和典型受害区土壤因子数据、气象数据等,采用MaxEnt模型对其在新疆的潜在地理分布及其主要影响因子进行预测和分析验证。结果表明:受试者工作特征曲线下面积(area under the ROC curve,AUC)都大于0.990,预测结果可靠。突颊侧琵甲的潜在分布区位于新疆天山一带,高、中度适生区分布在伊犁河谷草原和昌吉州玛纳斯县、呼图壁县草原及塔城地区沙湾县等地;刀切法得出降水量是影响其潜在地理分布的主要因素,特别是5、6、11、12月降水量的影响最大。该虫典型受害区的降水数据验证了分布概率响应曲线的准确性;土壤湿度在15%~30%范围时,30 cm处土壤湿度与虫口密度呈极显著弱正相关。表明降水作为影响草原土壤湿度、土壤含氧量的主要和直接因素,对不同生长期突颊侧琵甲生存起到重要作用。     

Strong differentiation within Diplocarpon rosae strains based on microsatellite markers and greenhouse-based inoculation protocol on Rosa

The haploid ascomycete Diplocarpon rosae is the causal agent of black spot disease on roses, a widespread and devastating disease in the outdoor landscape. In this study, we established a Eurasian collection of 77 monoconidial strains of D. rosae: 50 strains collected on cultivated roses in Europe and Asia, and 27 strains on wild roses in Kazakhstan. To provide tools to describe its biology and to study its genetic diversity, we sequenced two strains of D. rosae using Illumina paired-end technology. The genome sizes of these two strains were estimated at 31.1 and 35.2 Mb, which are two times smaller than the genome size of the unique strain previously published. A BUSCO analysis confirmed a genome duplication of the strain previously sequenced and partial gene duplication of strains analysed in this study. Using the two genome sequences, 27 polymorphic microsatellite markers were identified. Polymorphism analysis of the 77 strains revealed a strong genetic differentiation between strains from cultivated and wild roses, and a lower diversity within the fungal population from cultivated roses compared to the population from wild roses. Pathogenicity of 10 strains was evaluated on 9 rose cultivars inoculated in the greenhouse. Disease scoring allowed the classification of strains into three groups and the characterization of resistance of rose cultivars. Good correlation observed between resistance scoring in greenhouse conditions and in the field indicates that pathogenicity assays in controlled conditions could be very useful in the near future to rapidly characterize the resistance of new rose varieties to black spot disease.     

室内环境养蛹预测柑橘大实蝇成虫发生期

柑橘大实蝇Bactrocera minax是为害我国柑橘类果树的重要害虫。准确地预测柑橘大实蝇的羽化动态,对提高其成虫时期的防治效果具有重要意义。本试验以石门县为例,在2014年-2016年连续3年采用室内环境和柑橘园观察圃中养蛹的方法,逐日观察其羽化出土动态,计算室内环境较柑橘园观察圃中柑橘大实蝇羽化盛期的提前程度,并分析温度、湿度和降雨量等环境因素对提前程度的影响。结果显示,柑橘大实蝇的羽化出土动态受到温度、降水等环境因素的综合影响,温度较高降水充足的年份,成虫的羽化进度和羽化周期相应提前和缩短。但室内环境下柑橘大实蝇羽化盛期较柑橘园观察圃中具有相对稳定的提前天数,2014年-2016年室内环境下柑橘大实蝇羽化的始盛日、高峰日和盛末日分别比柑橘园观察圃中相应时期提前(5.7±1.2)d、(6.3±1.9)d和(7.7±2.1)d。而且提前程度不受温度、湿度和降雨量等环境因素的影响。因此,室内环境养蛹的方法可较为准确地预测柑橘大实蝇的羽化动态,从而指导柑橘大实蝇的成虫诱杀。     

A standard set of host differentials and unified nomenclature for an international collection of Diplocarpon rosae races

Black spot of rose is distributed throughout the world and is the most serious disease of roses (Rosa spp.) in the outdoor landscape. Resistance breeding has been frustrated by the occurrence of races of the causal pathogen Diplocarpon rosae. Races from Germany, North America and the UK have been characterized and maintained in a pathogenic state. However, these races were characterized using independent sets of host genotypes and are referenced using different nomenclatures. In the present study, a total of 15 D. rosae isolates from these locations, as well as Belgium and Italy, were inoculated to a common set of 15 rose cultivars in replicated, detached leaf trials. Baby Love^TM (cv. Scrivluv) was resistant to all isolates except for one originating from the UK. The rose cultivars Mrs Doreen Pike (Ausdor) and Hansa were resistant to all isolates except for one originating from Minnesota, USA. No rose genotype was universally susceptible. A total of 11 pathogenic races were differentiated based on their unique host ranges and were assigned an international race nomenclature. Nine cultivars are proposed as the first standard set of differential genotypes for characterization of D. rosae races.     

大蒜绿圆跳虫的发生规律与防治

绿圆跳虫是陕西省一种严重危害大蒜的新害虫。３月下旬至５月初虫口数量最多，田间最高有虫株率平均４０％，虫株平均有虫８头以上。虫口增长的适温为１５～２０℃，适宜相对湿度为５０％～７５％，高温高湿时虫口数量剧降     

Ecological Studies on the Larger Grain Borer, Prostephanus truncatus (Horn) (Col.: Bostrichidae) and their Implications for Integrated Pest Management

In this article, we review studies of the ecology of the larger grain borer, Protephanus truncatus, both outside and within the maize storage systems. Laboratory studies have shown that P. truncatus can breed on a wide range of woody substrates (branches, roots, and seeds). Pheromone trap catches in different habitats strongly suggest that P. truncatus is well-established in certain non-agricultural environments, presumably breeding in dead or dying wood. In Meso-America, West and East Africa, P. truncatus reproduction in the field has been documented in branches ring-barked by cerambycid beetles. Within a maize store, P. truncatus densities can increase from very small initial colonies of probably less than 200 individuals to densities in excess of 1000 beetles per kg after about 4–6 months of storage. Insect parasitoids are very often found in smallholders' grain stores attacked by P. truncatus, but they do not contribute significantly to population regulation. Declines in maize store population levels in Benin from 1993 to 1996, and in adult abundance in pheromone traps in the natural environment in Kenya, have been attributed to predation by the introduced predator, Teretrius nigrescens, but in recent years pheromone trap catches in West Africa suggest that the situation may be complex. Several environmental factors, notably temperature, humidity, and daylength, and their interactions, have been correlated with P. truncatus flight activity, as well as, in West Africa, the emptying of maize stores. Laboratory experiments have shown food quality also affects flight activity. Factors terminating dispersal and flight are most likely attraction to the male-released aggregation pheromone. Short range attraction to plant volatiles has also been recorded. In Africa the highest densities of P. truncatus tend to occur in humid lowlands, which contrasts with the situation in meso-America where P. truncatus tend to occur in greatest numbers in cooler upland regions. Pheromone trap catches can be significant predictors of the risk of stores becoming infested. Coupled with the development of a rule-based model of flight activity, these studies may offer the prospect of predicting the risk of store infestation based upon temperature and humidity measurements.     

A review of research to address carrot fly (Psila rosae) control in the UK1

Carrot fly (Psila rosae) is a serious pest of umbelliferous crops such as carrot, parsnip, celery and parsley. Carrot fly has been studied in the UK for more than 60 years. Whilst some of these studies have been focused on insecticides, others have demonstrated the potential for non‐insecticidal methods of control that might contribute to an integrated pest management strategy. These include rotation and spatial separation of susceptible crops; carrot varieties with partial resistance to carrot fly; and crop management strategies to avoid oviposition or the development of severe damage. This paper outlines some of the approaches that have been taken and indicates how these are, or might be, used in practice.     

不同环境因子对桔小实蝇生长发育的影响

桔小实蝇是一种危害250多种热带、亚热带水果和蔬菜的重要检疫性害虫,已经给农业生产造成了巨大的损失,影响其种群变化的因子很多。本文综述了温度、光照、湿度等环境因子对桔小实蝇生长发育影响的研究进展和现状,以期对温度、光照、湿度等环境因子对桔小实蝇生长发育的影响有个较为全面的认识,为进一步防治桔小实蝇的危害提供帮助。     

Review of carrot fly control in Northern Europe – 20091

In February 2009, the European and Mediterranean Plant Protection Organization (EPPO) held a workshop to discuss the management of carrot fly (Psila rosae) within Europe. Prior to the workshop, participants completed a questionnaire about carrot fly and current control methods and some of the information is summarised in this paper. In general, this pest is controlled adequately at present, but there is a heavy reliance on insecticides. The responses to the questionnaire highlighted a number of issues, in particular: the limited range and efficacy of the insecticides available at present, concerns about the availability of effective control measures in the future and a need for improvements in the targeting of treatments through forecasting and monitoring and the use of treatment thresholds.     

Factors affecting the virulence of Ralstonia solanacearum and its colonization on tobacco roots

Tobacco bacterial wilt caused by Ralstonia solanacearum is a serious disease affecting tobacco cultivation in southwest China. The response surface methodology was employed to evaluate the optimal conditions of tobacco bacterial wilt, and green fluorescent protein gene (gfp) labelling was applied to monitor the location and survival dynamics of R. solanacearum (Rs::gfp) on tobacco roots and in soil under these optimal conditions. The results showed that the highest wilt incidence was 91.13%, which occurred when the population reached 6.6 × 10⁶ CFU/g soil, the temperature was 30.55 °C, and the humidity was >81.42%. The Rs::gfp densely colonized the root tips and root hairs, and cells of Rs::gfp were observed intermittently in the elongation zone or at the point of the emerging lateral roots. The Rs::gfp number in the rhizosphere soil was 10.75‐, 73.13‐ and 74.86‐times higher than that in the bulk soil at 10, 15 and 20 days after transplantation, respectively. Increased colonization by Rs::gfp was related to the population of the pathogen, the environmental temperature and the humidity in the soil. These three conditions determined whether R. solanacearum would induce tobacco wilt. This is the first study to investigate factors affecting the virulence of a tobacco wilt bacterial pathogen, which is important for conducting field diagnosis and biocontrol of tobacco bacterial wilt.     

Pest control by pathogens — fungi, viruses and bacteria Control of chrysanthemum pests with verticillium lecanii

Integrated pest control on chrysanthemum is once again becoming a possibility.Verti-cillium lecanii is one of the key components as, under the right conditions, it will provide good control of several pest species. High humidity for a number of nights per week is critical for reliable pest control with this fungal pathogen. These conditions can be obtained easily and safely by fogging water over the crop at night. Four consecutive nights of high humidity per week or a cycling two nights of high humidity and two nights ambient have given excellent pest control with no adverse effect to the crop.     

Modelling regional level spatial distribution of aphid (Lipaphis erysimi) growth in Indian mustard using satellite-based remote sensing data

Abstract

We developed a procedure for preparing a model for mapping spatially distributed zones of aphid pest (Lipaphis erysimi) outbreaks at a regional level. This study employed near-surface meteorological parameters derived from National Oceanic and Atmospheric Administration (NOAA) Television and Infra-Red Operational Satellites (TIROS) Operational Vertical Sounder (TOVS) data and field observations of disease infestation. The study area comprised three sites representing semi-arid and sub-humid regions of dominant Indian mustard (Brassica juncea L.)-growing regions of India. A model based on TOVS-derived cumulative surface air temperature and minimum specific humidity (SpH) was developed to estimate the date of ‘aphid onset’ (first appearance), date of peak infestation and location of severity with respect to aphid population density. Aphid population growth rate during the linear growth phase between aphid onset to peak was computed using SpH-weighted temperature and dates of sowing of the crop (crop age). Sowing dates of mustard crop, of northwest India, were obtained from spectral growth profiles derived from time series remote sensing (RS) products of the SPOT-4 VEGETATION sensor. Estimated dates of peak aphid infestation and peak population showed a strong match with the observed data. The location of peak aphid population density was depicted in each spatial grid of 25×25 km² for parts of northwest India. The simulated aphid population build-up and date of peak population density was validated with observed data for an unknown site in the Sriganganager district, Rajasthan state, India. Comparison of predicted dates of attaining peak aphid population with observations showed a deviation of ±7 days. After validation, the regional level model was applied over a large area of a mustard-growing region for varying dates of sowing, surface air temperature and specific humidity, to show the spatial distribution of aphid growing severity zones (population density) and to predict dates of severe aphid infestation (peak population) at each grid level in the region.     

Population dynamics of citrus leaf miner on different varieties of citrus in correlation with abiotic environmental factors in Sargodha District,Punjab, Pakistan

From January 2010 to December 2011, samples of leaves from citrus varieties Kinnow, Musambi and Feutral were taken from the five tehsils (administrative subdivisions) of Sargodha District in Pakistan including Sargodha, Bahalwal, Silanwalli, Sahiwal and Kotmomin, to study the population trends in citrus leaf miner (CLM), Phyllocnistis citrella (Stainton) (Lepidoptera: Gracillariidae), and its correlation with various environmental factors: (temperature, humidity and rainfall); plant morphological factors: moisture contents of leaves, leaf thickness, surface area (cm ² ), and biochemical percentage of calcium, potassium and magnesium in leaves. The maximum population of CLM was observed on Kinnow and Feutral, followed by Musambi. The effect of these factors on the larval population was 8.39- 2.30(Mg)+2.73(K)-0.398(Ca)-0.100(Temp)0.038(Humidity)+0.567(Rain)+0.07(Moist) 1.01 (Thickness)-0.022(Surface area). This equation revealed that magnesium, calcium, temperature, humidity, leaf thickness and leaf surface area are negatively correlated with larvae population, whereas potassium, rainfall and moisture are positively correlated with larvae population.     

Elevated humidities for control of chrysanthemum pests with Verticillium lecanii

Integrated pest control on chrysanthemum is now a possibility. Verticillium lecanii (Zimm.) Viegas is one of the key components as, under the right conditions, it will provide good control of pests such as aphids, thrips and whitefly. High humidity for a number of nights per week is critical for reliable pest control with this fungal pathogen. These conditions can be easily and safely obtained by fogging water over the crop at night. Four consecutive nights of high humidity per week or a cycle of two nights of high humidity and two nights ambient have given excellent pest control with no adverse effect to the crop.     

Climatic Zoning and Plant Disease Potential – Examples from the Near and Middle East1

Actual plant disease and pest occurrence depends on many genetic and environmental factors, and frequently obscures the basic suitability of a given location to support or prevent epidemic development. In order to allow the demarcation of climatic zones related to the potential of disease or pest occurrence, we have used long-term average climatic data, especially monthly average temperatures and monthly average rainfall. If applied to sugar beet leaf pathogens such as Cercospora beticola and Erysiphe betae in the Near and Middle Eastern region, some interesting zoning became possible, which could be verified by extended field studies. Other examples that have been analysed in the region are apple scab, Venturia inaequalis, and downy mildew of grapes, Plasmopara viticola. A recent and ongoing analysis of the factors controlling chickpea anthracnose caused by Ascochyta rabiei indicates that the same principle may be applied for very different pathogens. Large-scale planning and control strategies as tried by the International Agricultural Research Centers should therefore be based on careful climatic zoning for plant pest and disease potential, to avoid waste of the limited genetic and financial resources available.     

The sensitivity of simulation models to temperature changes1

Insect population processes, such as development and reproduction, are dependent upon temperature. Accurate predictions of the phenology and population dynamics of insect pests require accurate recording of the temperatures experienced by the organisms. Any errors in the temperature measurements may result in spurious estimates of the pest population and, therefore, poor quality advice on the necessary control strategies. A simulation model predicting the phenology and population dynamics of the summer fruit tortrix, Adoxophyes orana, will be described. Output from the model closely resembled field observations of the population dynamics of the pest. Experimentation with the model revealed its sensitivity to small changes of temperature. The results illustrate the importance of using input recorded realistically with respect to the biology of the pests.