Reduced rates of tank mixtures for red sprangletop (Leptochloa chinensis [L.] Nees) and greater club-rush (Scirpus grossus [L.] f.) control in rice

A study on the efficacy of tank-mix combinations on the growth of Leptochloa chinensis (L.) Nees and Scirpus grossus (L.) f. at the 3–4-leaf stage was conducted under glasshouse conditions. Sethoxydim plus cyhalofop-butyl at 8 + 25, 16 + 25, 32 + 25, 32 + 12.5, and 32 + 6.25 g ai ha⁻¹ provided good control of L. chinensis , ranging from 89–95%. However, a comparison of the costs of herbicide mixtures revealed that sethoxydim at 32 g ai ha⁻¹ plus cyhalofop-butyl at 6.25 g ai ha⁻¹ were the most cost-effective among the combinations. For Scirpus grossus , bensulfuron plus 2,4-D dimethylamine at 2.5 + 18, 5.0 + 18, 1.25 + 36, 2.5 + 36, and 5.0 + 36 g ai ha⁻¹ gave good control that ranged from 88–93%. Tank-mixing bensulfuron with 2,4-D at 1.25 + 36 g ai ha⁻¹ was, however, the most cost-effective mixture compared to the other mixtures. None of the tank mixtures tested in this study caused injury to the rice seedlings. However, further field studies need to be undertaken to verify these findings.     

Responses of otebo bean (Phaseolus vulgaris L.) to postemergence herbicides

Ontario otebo bean growers have few herbicide options available for weed management. Six field trials were conducted in Ontario, Canada, over a 2 year period (2003 and 2004) to evaluate the tolerance of otebo bean to the postemergence (POST) application of bentazon at 1080 and 2160 g ai ha⁻¹, fomesafen at 240 and 480 g ai ha⁻¹, sethoxydim at 500 and 1000 g ai ha⁻¹, quizalofop-p-ethyl at 72 and 144 g ai ha⁻¹, imazamox plus fomesafen at 25 + 200 and 50 + 400 g ai ha⁻¹, and imazamox plus bentazon at 25 + 600 and 50 + 1200 g ai ha⁻¹. All treatments, including the untreated control, were maintained weed-free during the growing season. The POST application of bentazon, imazamox plus fomesafen, and imazamox plus bentazon caused as much as 9% visual injury and reduced the plant height ≤ 12%, reduced the shoot dry weight ≤ 32%, and delayed maturity but had no adverse effect on the yield of otebo bean. Fomesafen, sethoxydim, and quizalofop-p-ethyl applied POST caused as much as 8% visual injury but this was transient and had no adverse effect on the plant height, shoot dry weight, seed moisture content, and yield of otebo bean, except for quizalofop-p-ethyl, which reduced the shoot dry weight as much as 18%. Based on these results, bentazon, fomesafen, sethoxydim, quizalofop-p-ethyl, imazamox plus fomesafen, and imazamox plus bentazon applied POST have an adequate margin of crop safety for weed management in otebo bean production in Ontario. However, care must be taken to avoid spray overlaps to prevent injury from bentazon, imazamox plus fomesafen, and imazamox plus bentazon.     

Response of winter wheat (Triticum aestivum L.) and weeds to tank mixtures of 2,4-D plus MCPA with clodinafop propargyl

Field and greenhouse experiments were conducted in 2004 and 2005 to study weed control and the response of winter wheat to tank mixtures of 2,4-D plus MCPA with clodinafop propargyl. The field experiments were conducted at Yazd and Oroumieh, Iran, with factorial combinations of 2,4-D plus MCPA at 0, 975, and 1300 g ai ha⁻¹ and with clodinafop propargyl at 0, 64, 80, 96, and 112 g ai ha⁻¹ in four replications. The greenhouse experiments further evaluated the effect of these tank mixtures on weed control, where each herbicide mixture was considered as one treatment and the experiment was established in a randomized complete block design with four replications. In the field experiments, the herbicides were applied at wheat tillering, while in the greenhouse experiments they were applied at the beginning of the tillering stage and at the four-leaf stage of the grass and broadleaf weeds, respectively. The results indicated antagonistic effects between 2,4-D plus MCPA and clodinafop propargyl. The best tank mixture with regard to weed control efficacy was 2,4-D plus MCPA at 975 g ai ha⁻¹ with clodinafop propargyl at 96 g ai ha⁻¹. The wheat grain yield was also increased by the tank mixture of clodinafop propargyl with 2,4-D plus MCPA. Generally, to inhibit clodinafop propargyl efficacy reduction due to tank-mixing with 2,4-D plus MCPA, it is recommended that the application dose of 64 g ai ha⁻¹ should be increased to 96 g ai ha⁻¹.     

Control of weed barley species in winter wheat with sulfosulfuron at different rates and times of application

Field experiments were conducted at five locations in the major wheat production regions of Iran to evaluate the efficacy of sulfosulfuron in controlling weed barley species (including Hordeum spontaneum , Hordeum murinum , Hordeum distichon , and Hordeum vulgare ) in the 2004–2005 and 2005–2006 growing seasons. Sulfosulfuron was applied either postemergence (POST) or preplant-incorporated (PPI) at 0, 20.25, 30.75, 40.5, 51.0, 60.75 or 71.25 g ai ha⁻¹ to plots arranged in a randomized complete block design with four replications. Sulfosulfuron at the recommended rate (20.25 g ai ha⁻¹) failed to provide acceptable control of the weed barley species. However, the level of control increased with the application rate, particularly at rates >51.0 g ai ha⁻¹. Generally, PPI-applied sulfosulfuron resulted in markedly greater control levels than those of a POST application and complete control of H. murinum and H. vulgare was achieved with PPI-applied sulfosulfuron at all rates >20.25 and 30.75 g ai ha⁻¹, respectively. In most cases, the wheat yield increased with the application rate without any crop injury. The highest yield increase (186%) was obtained with a PPI application of 71.25 g ai ha⁻¹.     

Synthesis and herbicidal activity of new 3-(3-bromothiophen-2-yl)-5-(2,6-difluorobenzyl)oxymethyl-5-methyl-4,5-dihydroisoxazole as a paddy field herbicide

A new compound 3-(3-bromothiophen-2-yl)-5-(2,6-difluorobenzyl)oxymethyl-5-methyl-4,5-dihydroisoxazole was synthesized and the herbicidal activity was assessed under glasshouse and flooded paddy field conditions. This compound demonstrated good rice selectivity and potent herbicidal activity against annual weeds at 125 g ai ha⁻¹ under greenhouse conditions. Soil application of this compound showed the complete control of barnyardgrass to the 4th leaf stage at 250 g ai ha⁻¹. Field trials indicated that this compound, combined with pyrazosulfuron-ethyl, controlled annual and perennial weeds rapidly with good tolerance by transplanted rice seedlings from postemergence and soil application. This compound showed a low mammalian and environmental toxicity in various toxicological tests.     

White bean (Phaseolus vulgaris) tolerance to preplant-incorporated herbicides

There is a limited number of registered herbicides in white beans. Field trials were conducted at two Ontario, Canada, locations (Exeter and Ridgetown) in 2001 and 2002 to evaluate tolerance of two white bean cultivars, AC Compass and OAC Thunder, to preplant-incorporated applications of S -metolachlor plus imazethapyr (1600 + 75 and 3200 + 150 g ai ha⁻¹, respectively), flumetsulam plus S -metolachlor ( premixed at 1443 and 2886 g ai ha⁻¹) and cloransulam-methyl (35 and 70 g ai ha⁻¹). There were no differences between the two cultivars in their responses to the herbicide treatments. S -metolachlor plus imazethapyr caused as much as 5% visual crop injury and decreased plant height up to 20%, shoot dry weight up to 39% and yield as much as 21%. Flumetsulam plus S -metolachlor caused as much as 7% visual crop injury and reduced plant height by up to 25%, shoot dry weight by up to 46% and yield as much as 24%. Cloransulam-methyl caused as much as 10% visual crop injury and decreased plant height up to 35%, shoot dry weight up to 55% and yield as much as 44%. There were no differences in seed moisture content among any of the herbicide treatments. This research suggests that the margin of safety of white bean is inadequate to support the preplant-incorporated registration of S -metolachlor plus imazethapyr, flumetsulam plus S -metolachlor and cloransulam-methyl in Ontario.     

Response of black, cranberry, kidney, and white bean to linuron

Dry bean producers in Ontario, Canada, have few herbicide options available for annual broad-leaved weed management and there is little information on the tolerance of dry bean to linuron. The tolerance of black, cranberry, kidney, and white bean to the pre-emergence (PRE) application of linuron at the rates of 0, 500, 1000, 1500, 2000, and 2500 g ai ha⁻¹ was evaluated in field studies conducted in 2005 and 2006 at Exeter and in 2006 at Ridgetown, Ontario. The four market classes differed in their response to linuron. Cranberry and kidney bean were more tolerant to the PRE application of linuron than black and white bean. Linuron applied PRE caused as much as 12% injury in cranberry and kidney bean, 47% injury in black bean, and 56% injury in white bean. Linuron applied PRE at 500–2500 g ai ha⁻¹ had no effect on the height of cranberry and kidney bean but decreased the height by 7, 8, and 15% in black bean and by 10, 13, and 23% in white bean at 1500, 2000, and 2500 g ai ha⁻¹, respectively. Linuron applied PRE at the rates evaluated did not cause any adverse affect on the yield of cranberry, kidney, and white bean but black bean yield was reduced by 16% at 2500 g ai ha⁻¹. Based on these results, there is not an adequate margin of crop safety for the PRE application of linuron in black and white bean at rates >1000 g ai ha⁻¹. However, there is a potential for the use of linuron PRE for weed management in cranberry and kidney bean at the rates evaluated.     

Tolerance of black, cranberry, kidney, and white bean to cloransulam-methyl

The level of tolerance of various market classes of dry bean to cloransulam-methyl is not known. Three field studies were conducted in Ontario, Canada during 2007 and 2008 to determine the level of tolerance of black, cranberry, kidney, and white bean to the pre-emergence (PRE) and postemergence (POST) application of cloransulam-methyl at 17.5, 35, and 70 g ai ha⁻¹. Cloransulam-methyl applied at 17.5, 35, and 70 g ha⁻¹ caused between 13 and 23% injury in black, cranberry, kidney, and white bean, respectively. Cloransulam-methyl applied at 17.5, 35, and 70 g ha⁻¹ reduced the shoot dry weight by between 16 and 28% compared to the untreated control. Cloransulam-methyl applied PRE reduced the height of black bean by 27% and the height of cranberry bean by 25% at 70 g ha⁻¹ and reduced the height of white bean by 19% at 35 g ha⁻¹ and by 37% at 70 g ha⁻¹. Cloransulam-methyl applied PRE reduced the yield of black bean by 29% at 35 g ha⁻¹ and by 43% at 70 g ha⁻¹, reduced the yield of cranberry bean by 43% at 70 g ha⁻¹, and reduced the yield of white bean by 36% at 35 g ha⁻¹ and by 54% at 70 g ha⁻¹. Based on these results, there is not an adequate margin of crop safety for the PRE and POST application of cloransulam-methyl in black, cranberry, kidney, and white bean at the rates evaluated.     

Optimizing the performance of diclofop-methyl, cycloxydim, and clodinafop-propargyl on littleseed canarygrass (Phalaris minor) and wild oat (Avena ludoviciana) control with adjuvants

Optimizing the herbicide dose by the addition of adjuvants is an acceptable way to reduce the risk of side-effects from herbicides. Therefore, to detect a suitable adjuvant for diclofop-methyl, cycloxydim, and clodinafop-propargyl against littleseed canarygrass ( Phalaris minor ) and wild oat ( Avena ludoviciana ), six dose–response experiments were conducted. The treatments consisted of diclofop-methyl at 0, 112, 225, 450, 675, and 900 g ai ha⁻¹, cycloxydim at 0, 15, 30, 60, 90, and 120 g ai ha⁻¹, and clodinafop-propargyl at 0, 8, 16, 32, 48, and 64 g ai ha⁻¹ with and without the adjuvants of Frigate, olive oil, and castor oil at 0.2% (v/v) in order to control both littleseed canarygrass and wild oat. Tested herbicides performance was enhanced by all adjuvants against littleseed canarygrass and wild oat. The addition of Frigate and the vegetable oils had the lowest and the highest effect on the performance of all of the herbicides on both littleseed canarygrass and wild oat, respectively, which confirms the solubilizing nature of the cuticular waxes by vegetable oils. A comparison between the two vegetable oils revealed that olive oil exerted a greater control of littleseed canarygrass than did the castor oil. In contrast, castor oil exerted a greater control of wild oat than did the olive oil, which can be related to differences in the leaf surface micromorphology of the weeds.     

Weed control in summer-sown soybeans with flumioxazin plus acetochlor and flumiclorac-pentyl plus clethodim

Field trials were conducted in Taigu, Shanxi province, China, to evaluate the efficacy of flumioxazin plus acetochlor and flumiclorac-pentyl plus clethodim applied to summer-sown soybeans at pre- and postemergence. It was demonstrated that tank-mixing flumioxazin at 50 g ai ha^-1 and acetochlor at 800 g ai ha^-1 created an effective soil-applied herbicide for weed control in soybean crops. The control efficacy was better than when the herbicides were applied individually, and no injury was caused to the soybeans. Flumiclorac-pentyl at 50 g ai ha^-1 plus clethodim at 70 g ai ha^-1 suppressed both broad-leaved weeds and grass weeds with an increased efficacy of more than 90%. Flumiclorac-pentyl applied alone or tank-mixed caused some injury to soybean seedlings, but the soybeans recovered 2–3 weeks after treatment and there was no reduction in the yield.     

Enhanced effect of thiobencarb on bispyribac-sodium control of Echinochloa phyllopogon (Stapf ) Koss. in California rice (Oryza sativa L.)

Bispyribac-sodium {sodium 2,6-bis[(4,6-dimethoxy-2-pyrimidinyl)oxy] benzoate} has recently been introduced to California where it effectively controls Echinochloa spp. in rice ( Oryza sativa L.). However, biotypes of early watergrass ( Echinochloa oryzoides (Ard.) Fritsch) and late watergrass ( E. phyllopogon (Stapf ) Koss.) have evolved resistance to this herbicide. In 2001 and 2002, greenhouse and field experiments evaluated interactions between thiobencarb { S -[(4-chlorophenyl) methyl] diethylcarbamothioate} and bispyribac-sodium on resistant (R) and susceptible (S) late watergrass in California rice. Synergism was assessed using Colby's test and regression analysis. In the greenhouse, thiobencarb at 4480 and 5333 g ai ha⁻¹ synergistically reduced bispyribac-sodium GR₅₀ values on the R and S biotypes by 50–70% without increasing toxicity to rice. Synergism was also observed on S late watergrass in the field when 10 g ai ha⁻¹ bispyribac-sodium was mixed with 1120–2240 g ai ha⁻¹ thiobencarb. These effects could be related to interactions between thiocarbamates and enzymes in Phase I reactions of herbicide metabolism. This synergism results in better control at lower rates allowing a reduction in weed control costs, the herbicide load on the environment and a lower selection pressure towards resistant weed biotypes.     

Control of the invasive liana, Hiptage benghalensis

The liana, hiptage ( Hiptage benghalensis ), is currently invading the wet tropics of northern Queensland and remnant bushland in south-eastern Queensland, Australia. Trials using seven herbicides and three application methods (foliar, basal bark, and cut stump) were undertaken at a site in north Queensland (158 700 hiptage plants ha⁻¹). The foliar-applied herbicides were only effective in controlling the hiptage seedlings. Of the foliar herbicides trialed, dicamba, fluroxypyr, and triclopyr/picloram controlled >75% of the treated seedlings. On the larger plants, the cut stump applications were more effective than the basal bark treatments. Kills of >95% were obtained when the plants were cut close to ground level (5 cm) and treated with herbicides that were mixed with diesel (fluroxypyr and triclopyr/picloram), with water (glyphosate), or were applied neat (picloram). The costings for the cut stump treatment of a hiptage infestation (85 000 plants ha⁻¹), excluding labor, would be $A14 324 ha⁻¹ using picloram and $A5294 ha⁻¹ and $A2676 ha⁻¹, respectively, using glyphosate and fluroxypyr. Foliar application using dicamba for seedling control would cost $A1830 ha⁻¹. The costs range from 2–17 cents per plant depending on the treatment. A lack of hiptage seeds below the soil surface, a high germinability (>98%) of the viable seeds, a low viability (0%) of 2 year old, laboratory-stored fruit, and a seedling density of 0.1 seedlings m⁻² 12 months after a control program indicate that hiptage might have a short-term seed bank. Protracted recolonization from the seed bank would therefore be unlikely after established seed-producing plants have been controlled.     

Comparison of different weed management systems and their effects on yield of coconut plantations in Sri Lanka

The influence of five different weed management systems on nut yield of coconut were evaluated to determine an economical and effective method of controlling weeds in coconut plantations in the low country, dry zone in Sri Lanka. Treatments imposed included slashing and mulching around the palms with slash (T1), slashing and removing the slash (T2), application of glyphosate (N-(phosphonomethyl)-glycine) alone at 1.44 kg ai ha⁻¹ (T3), application of glyphosate alone at 2.88 kg ai ha⁻¹ (T4) and cover cropping with Pueraria phaseoloides (T5). All treatments were applied twice a year, except for the cover cropping treatment, T5. Based on a reduction in weed biomass, treatments T3, T4 and T5 were found to be significantly effective over other treatments. Coconut yield was increased significantly ( P  < 0.05) in glyphosate-applied plots at both tested rates. Control of weeds with the lower concentration of glyphosate (1.44 kg ai ha⁻¹) resulted in a 25% increase in nut yield over the uncontrolled weed plots. At this rate, it was found to be the most effective and economical method of controlling weeds in coconut plantations. Cover cropping with Pueraria phaseoloides was effective in controlling weeds in the long-term, but was not economical compared with the glyphosate application.     

Biological activity of pyribenzoxim in winter wheat and associated weeds

Pot and field tests were conducted to evaluate the efficacy of pyribenzoxim for winter weeds in wheat. In the pot tests, pyribenzoxim, at 50 g ha⁻¹, controlled certain biotypes of blackgrass, including a fenoxaprop-P-ethyl-resistant biotype (the "Notts" biotype). A chlorotoluron-resistant blackgrass (the "Peldon" biotype) was not controlled. Cleaver, at the three-to-four-leaf stage, was completely controlled by pyribenzoxim at 30 g ha⁻¹. In the field, the application in December gave good control of common chickweed, but did not control other weeds. No damage to wheat was observed with this rate of pyribenzoxim in December. The application in March gave complete control of blackgrass , hairy chess, and soft brome at 70 g ha⁻¹, and cleaver at 140 g ha⁻¹. The partial control of corn poppy and field violet was achieved. The March application scorched the wheat at 50–70 g ha⁻¹, with prolonged stunting at 100–140 g ha⁻¹. In conclusion, it was shown that pyribenzoxim had potential as a wheat herbicide, but needed further fine-tuning to find an optimum dosage.     

Susceptibilities of some aquatic ferns to paddy herbicide bensulfuron methyl

Paddy herbicides have the potential to cause adverse effects on non-target plants. Susceptibilities of some aquatic ferns ( Azolla japonica Franch. et Savat., Isoetes japonica A. Braun, Marsilea quadrifolia L. and Salvinia natans All.) and duckweeds ( Lemna minor L. and Spirodela polyrhiza Schleid.) to paddy herbicide bensulfuron methyl (BSM) were evaluated with a 20 day exposure experiment using 200 cm² pots. The BSM concentrations in the surface water of monitoring pots with no plants dissipated exponentially with half lives of 3.5 and 3.9 days at application rates of 15 and 150 g ha⁻¹, respectively. The BSM concentrations in the surface water 1 day after application in the culture pots were comparable among plant species, and were lower than those in the monitoring pots. Bensulfuron methyl reduced the plant growth in all species. I . japonica showed the lowest intrinsic relative growth rate (RGR) and the lowest susceptibility with an effective dose resulting in 50% growth inhibition (ED₅₀) of 21 g ha⁻¹. Except for I . japonica , the RGR of the duckweeds was similar to the ferns, and ED₅₀ for the duckweeds was higher than the ferns. ED₅₀ for Sa . natans , A . japonica and M . quadrifolia were 1.1, 1.8 and 1.2 g ha⁻¹, respectively, which were smaller than 1/20 of the recommended field dose (51–75 g ha⁻¹) and ranged from 1/2 to 1/6 of ED₅₀ for L . minor and Sp . polyrhiza (6.5 and 3.2 g ha⁻¹, respectively). These results suggest that BSM application in paddy fields and its runoff in some localities is expected to have adverse effects on the growth of Sa . natans , A . japonica and M . quadrifolia .     

Weed infestation and tea growth under various weed management methods in a young tea (Camellia sinensis[L.] Kuntze) plantation

A field experiment was conducted in the low country of Sri Lanka, during the period 1994–1995 to investigate the severity of weed infestation and tea growth in relation to weed management methods in newly established tea ( Camellia sinensis [L.] Kuntze). Manual weeding (hand and slash weeding) at various intervals was compared with various herbicides, with or without mulching. Weed control with herbicides was superior to that of hand weeding at 6-week intervals or more. Weed control with oxyfluorfen at 0.29 kg ai ha⁻¹ + paraquat at 0.17 kg ai ha⁻¹ or glyphosate at 0.99 kg ai ha⁻¹ + kaolin at 3.42 kg ha⁻¹ were superior. Plots unweeded for 12 weeks or more produced significantly greater ( P  < 0.05) weed biomass than plots unweeded for 6 weeks. Although the least weed dry weight ( P  < 0.05) and the greatest number of weed species were recorded with hand weeding at 2 week intervals, there was no particular benefit on tea growth when compared with hand weeding at 6 and 12 week intervals. Inter row mulching in chemically treated plots was more favorable for tea growth than no mulching, while living weed cover in unmulched slash weeded plots suppressed tea growth. A combination of mulching and herbicides, particularly oxyfluorfen and paraquat, followed by hand weeding at least every 6–8 weeks was considered the most appropriate weed management system for young tea.     

Red rice (Oryza sativa L.) and barnyardgrass (Echinochloa spp.) biotype susceptibility to postemergence-applied imazamox

Pot experiments were conducted to evaluate the level of imazamox tolerance in five red rice ( Oryza sativa L.) and four barnyardgrass (three Echinochloa crus-galli (L.) Beauv. and one Echinochloa oryzoides (Ard.) Fritch) morphologically distinct biotypes collected from rice fields in northern Greece. The susceptibility of barnyardgrass biotypes to propanil was also studied. Red rice biotypes were not controlled by imazamox applied at 40 g ha⁻¹. In contrast, 80 g imazamox ha⁻¹ provided 56–84% red rice control (averaged across shoot number and fresh weight reduction). Not all barnyardgrass biotypes were susceptible to imazamox applied postemergence. However, propanil applied at 2.6 kg ha ⁻¹ controlled the E. crus-galli biotypes well, but propanil applied at rates of 2.6 and 5.2 kg ha ⁻¹ was not effective in reducing the shoot number and fresh weight of the E. oryzoides biotype. Propanil applied at 10.4 kg ha ⁻¹ reduced the shoot number and fresh weight of this biotype by 78 and 85%, respectively. In most cases, a linear equation ( y  = % of control, x  = g ha⁻¹) provided the best fit for regressions between red rice or barnyardgrass shoot number or fresh weight and imazamox rates. The results of this study suggest that postemergence application of imazamox is not effective against all red rice and barnyardgrass biotypes found in the rice fields of Greece and that significant variability regarding herbicide efficacy among biotypes might exist.     

Orobanche aegyptiaca control in tomato fields with sulfonylurea herbicides

Field experiments were conducted from 1994 to 1997 at two locations to study the effectiveness of chlorsulfuron and triasulfuron applied through different irrigation methods (chemigation) for control of Orobanche aegyptiaca Pers. in tomato ( Lycopersicon esculentum Mill). Three split applications of chlorsulfuron at 2.5 g a.i. ha⁻¹ and of triasulfuron at 7.5 g a.i. ha⁻¹, through conventional sprinkler irrigation systems, 10–14 days apart followed immediately by sprinkling with water, controlled O. aegyptiaca by about 90% and 80% and increased crop yield 25–47% and 30%, respectively, without any crop injury symptoms. Repeated applications of the same herbicides at half rates resulted in slightly higher O. aegyptiaca control and crop yield than only one herbicide application at double rate. Chemigation by the sprinkler systems (microsprinklers, 60 m³ ha⁻¹) slightly increased the herbicide efficiency as compared with the high volume spray (800 m³ ha⁻¹). O. aegyptiaca control from sulfonylureas applied by drip chemigation was poor, as this probably requires very accurate timing and the herbicide distribution in the soil was not uniform.     

Management of late leaf spot of groundnut (Arachis hypogaea) with chlorothalonil-tolerant isolates of Pseudomonas aeruginosa

Fifteen groundnut-associated bacterial isolates that inhibited by > 90% the in vitro conidial germination of Phaeoisariopsis personata , causal agent of late leaf spot disease of groundnut, were applied as a prophylactic spray (10⁸ cfu mL⁻¹) and tested for control of the disease in the glasshouse. Two groundnut seed-associated bacterial isolates, GSE 18 and GSE 19, identified as Pseudomonas aeruginosa , reduced the lesion frequency (LF) by up to 70%. A 90-day-old peat-based formulation of P. aeruginosa GSE 18 reduced LF measured 15 days postinoculation by up to 60%. Both P. aeruginosa GSE 18 and GSE 19 were tolerant to chlorothalonil (Kavach®) up to 2000  µ g mL⁻¹ in LB broth. In glasshouse trials, GSE 18 and GSE 19 tested in combination with reduced concentrations of chlorothalonil were highly efficient in management of the disease. The disease was completely controlled by chlorothalonil (> 250  µ g mL⁻¹), and in the presence of GSE 18 or GSE 19, 100  µ g mL⁻¹ chlorothalonil was equally effective. Application of rifamycin-resistant mutants of GSE 18 or GSE 19 together with chlorothalonil significantly increased the survival of these isolates in the groundnut phylloplane. In the field, a combination of GSE 18 and 500  µ g mL⁻¹ chlorothalonil reduced disease severity comparable to 2000  µ g mL⁻¹ chlorothalonil alone. Use of chlorothalonil-tolerant pseudomonads together with a quarter concentration of the recommended field dose of chlorothalonil doubled pod yield compared with the untreated unsprayed control.     

Antifungal activity of bergenin, a constituent of Flueggea microcarpa

The antifungal activity of bergenin against some plant pathogenic fungi, namely, Alternaria alternata , A. brassicae , A. carthami , Fusarium udum , F. oxysporum f.sp. ciceri , Curvularia lunata and Erysiphe pisi , was studied. Bergenin as its monosodium salt was effective against all the fungi and the effective dose for complete inhibition of spore germination varied from 15 μg mL⁻¹ for F. udum to 125 μg mL⁻¹ for E. pisi . Experiments on the effect of bergenin on powdery mildew development under glasshouse conditions revealed that it can control powdery mildew of pea at 2000 μg mL⁻¹ by postinoculation treatment, the results being comparable with those of carbendazim (1000 μg mL⁻¹) and wettable sulfur (2000 μg mL⁻¹). It affected hyphal elongation and the number of primary and secondary branches.