鱼道进口布置方案研究

鱼道进口的布置除受其自身布置原则的限制之外，还受到枢纽布置及河势、地形、地质、下游水位等其他边界条件的限制。为同时满足以上双重要求，对某工程鱼道进口的布置进行深入研究，提出一种能满足边界条件及绝大部分布置原则的初步布置方案。对该初步布置方案中不能满足布置原则的采取相应的工程措施加以优化。水工模型验证表明，优化后的鱼道进口布置方案对河道中的洄游鱼类具有较大的吸引能力，在流量、流态、水深及流速等方面，均能较好地满足设计要求。     

西藏狮泉河鱼道设计初探

狮泉河水电站是西藏狮泉河流域梯级开发的第一座水电站,对水域生境和鱼类资源的影响巨大。为了缓解水利工程对鱼类资源带来的不利影响,通过对狮泉河水电站参数、水电站阻隔鱼类资源的初步分析,提出了狮泉河应修建鱼道来保护鱼类的方案。作者就狮泉河鱼道的过鱼对象、类型、主要结构设计特点等作了简要介绍。     

我国过鱼设施现状分析及鱼道适宜性管理的关键问题

水电开发对于洄游性鱼类产生的负面影响最为直接，阻碍了其繁殖、索饵以及越冬等生命行为。在调查的25个水电项目中，有17个采取了鱼类保护措施，其中采取增殖放流措施的占82.4%，仅有1个项目修建了鱼道。介绍了“莱茵河-2000计划”和哥伦比亚河鱼道建设及运行效果。鱼道不单纯是洄游性鱼类的通道，更是实现大坝上下游物质与能量循环的生态廊道。国内开展鱼道适应性管理的关键问题是：建立鱼道长效运行与保障机制，对鱼道过鱼进行长效监测以及研究鱼道过鱼效果评价方法。     

异侧布置竖缝式鱼道紊流结构试验研究

鱼道是辅助鱼类克服障碍物,实现产卵洄游、索饵洄游和越冬洄游的通道。全球目前已建鱼道中,鱼类能够溯游通过的鱼道尚不足50%,大多数鱼道的水力设计仅考虑平均流速,忽略了鱼道内的紊流结构,探究鱼道的水力特性尤其是紊流结构,对改进其设计具有重要的指导意义。通过在大比尺水槽模型中试验研究异侧布置竖缝式鱼道的紊流结构,利用声学多普勒测速仪(ADV)实测竖缝式水池内每一点的三维瞬时流速,剖析流场的三维时均流速分布、流动特征、旋涡特性、纵向和横向紊动强度分布、不同水平面的雷诺应力分布等,考虑了两种典型流量(Q1=20.62 L/s,Q2=30.75 L/s)和3个特征水平面(h/3,h/2和2h/3),提出了三维流速分布的半理论半经验公式、流场矢量图及各点的旋度,并与自由壁面射流进行比较。结果表明,异侧布置竖缝式鱼道前半池内纵向流速具有壁面射流的特征,后半池由于受下游隔板的阻挡则偏离壁面射流规律;水池内存在顺时针方向的水平旋涡,其旋度随流量的增大而增强;前半池壁面射流区的紊动强度存在峰值区,后半池的紊动强度峰值区由水槽左侧移至右侧;壁面射流区的雷诺应力变幅较大,而在旋涡区则较小,有利于洄游鱼类在鱼道上溯和歇息。     

一种鱼道进口诱鱼结构装置的试验研究

常规鱼道的研究和设计大多侧重于其内部结构和水流条件等方面,对鱼道进口位置布置及结构优化的研究相对较少,往往存在鱼类发现并进入鱼道较为困难的情况。通过建立鱼道进口设计的生态水力学指标,提出了某项目集诱鱼进口所需要满足的流速范围为0.47～1.30 m/s,将鱼类游泳能力与鱼道进口流态指标相结合,用标准三维κ-ε模型数值模拟的方式,对以往鱼道进口位置的选择原则进行了验证,提出了一种可控流量、流速的新型诱鱼补水装置,对鱼道进口结构进行优化。该诱鱼补水装置利用局部水流落差,上游发电下泄水流进行诱鱼补水,避免采用自库区压力管道引水而产生的消能问题,降低了鱼道运行的成本;同时降低机组满发或发电流量较高时河段内的流速,可达到灵活调控诱鱼水流进而诱导鱼类进入鱼道完成洄游的效果,最终完成鱼道进口的优化,提高过鱼效果。     

我国又一座鱼道建成

<正> 洋塘鱼道,是一座低水头水电站枢纽型鱼道,它位于湖南省湘江中游的主要支流洙水下游衡东县境的杨梓洲。电站下距湘江河口8.7公里。洙水全长327公里,宽400多米。它是湘江中下游鱼类索饵洄游产卵和越冬的主要场所。由于修建河坝,切断了鱼类上溯洄     

洋塘鱼道过鱼的昼夜、季节变化的初步研究

前言鱼道是保持鱼类洄游路线畅通的水工建筑物之一。它对解决江湖阻隔,保持和增殖天然鱼类资源,保持水域必要的生态环境具有一定的作用。洋塘鱼道是一座具有厂房集鱼系统的河川水电站枢纽型鱼道,位于洋塘水轮泵水电站枢纽工程的左侧,该工程座落在湖南省衡东县境内洣水下游之杨梓洲头,下距湘江河口8.7公里。 1981—1982年期间,我们对洋塘鱼道的运行进行了145天,2814 1/3小时的原体观察,总计过鱼1279645尾,隶属于5目13科或亚科,33属,45种。     

连江西牛鱼道运行效果的初步研究

鱼道建设被认为是有效缓解水坝阻隔鱼类洄游的重要方法之一，而鱼道效果监测是评价其功能的重要环节。连江西牛鱼道是广东省第一座建立在水坝主体上的过鱼通道，为了解其运行效果，采用张网法和截堵法，２０１２年３－８月共６次对西牛鱼道的过鱼效果进行监测。研究表明，西牛鱼道共监测到鱼类３目、８科、３０属、３８种，以银鮈（Squalidus argentatus）、乐山小鳔鮈（Microphysogobio kiatingensis）、子陵吻鰕虎（Rhinogobit giurinus）等小型鱼类为优势类群。进入鱼道的鱼类呈现昼夜差异，上午集鱼效果要显著优于其他时间段；不同季节集鱼的种类和数量呈现较大差别，３－８月集鱼数量逐渐减少，集鱼种类数以５月最多。与国内外其他鱼道的过鱼效果比较，西牛鱼道能较好地发挥其功能。     

不同位置和朝向的鱼道进口诱鱼效果

为了探明不同进口朝向的布置方式和进口位置对诱鱼效果的影响，本研究通过建立鱼道进口概化模型，设计3种环境流速工况，以马口鱼为实验对象，针对不同朝向和不同位置鱼道进口分别进行放鱼实验。结果显示，在同一鱼道进口位置时，垂直于河道水流方向上的鱼道进口其诱鱼效果明显优于顺河道水流方向的鱼道进口；当开启与电站不同相对位置不同的进口时，其诱鱼效果受河道水流速度和进口位置的影响较为显著；在所设计实验工况中，环境流速为0.3 m/s时，鱼道进口诱鱼效果最好。研究结果将对水利工程中鱼道进口布置具有重要的参考意义并指导工程实践。     

根据等深线推测定置网之鱼道

等深线就是深度等值线，鱼道是鱼群通过之路线。对于定置网捕鱼来说，等深线是定置网附近的鱼类洄游路线，它比其它渔业的鱼游路线狭窄。定置网捕捞是被动捕捞，捕捞的成功与否决定于网的敷设地点和网墙相对于鱼道的敷设方向。决定沿岸水域鱼道位置有4种因素：(1)等深线的特征，(2)海底天然礁的分布，(3)海底地形和海流之间的关系，     

Protein and amino acid composition from the mantle of juvenile O ctopus vulgaris exposed to prolonged starvation

Protein and amino acid composition of the mantle of juvenile O ctopus vulgaris (Cuvier, 1797) during fasting for 27 days were determined. Average protein content of octopus mantle was of 711.19 ± 46.80 g kg^?1 DW, and it decreased with increasing fasting days. The non‐essential amino acids content was higher (486.18 ± 11.08 g kg^?1 protein) than essential amino acids (425.82 ± 9.15 g kg^?1 protein) at the start of the experiment (unstarved animals). The results suggest that the amino acid profile of the mantle where the most abundant amino acids are Arg, His, Lys, Gly, Leu and Pro could indicate a prolonged fasting condition (>20 days) or poor nutrition of O . vulgaris. This study supports the idea of using mantle for metabolic needs of starved O . vulgaris suggesting that the degradation pathway of amino acids to pyruvate and tricarboxylic acid cycle intermediates was favoured contrary to the degradation pathway of ketogenic amino acids. Special considerations should be taken concerning Thr, Ile, Ser, Ala, Asx (Asp, Asn), Glx (Glu, Gln) (because of their fast intake) and Lys and His (due to their stable contents) during a prolonged period of fasting.     

美人鱼发光杆菌对凡纳滨对虾非特异性 免疫功能及抗病力的影响

在基础饲料中分别添加0.1%和1%美人鱼发光杆菌灭活菌、0.1%美人鱼发光杆菌活菌配制成3种免疫实验饲料,以基础饲料为空白对照组饲料,每组设3个平行样。对个体质量为(4.83±0.36)g的凡纳滨对虾进行为期20 d的饲养实验,分别在0、5、10、15和20d进行取样,以血清中的酚氧化酶(PO)、酸性磷酸酶(ACP)、碱性磷酸酶(AKP)、超氧化物歧化酶(SOD)和溶菌酶(UL)活性为免疫指标,探讨了美人鱼发光杆菌作为免疫制剂对凡纳滨对虾非特异性免疫效应的影响;在投喂免疫饲料后的第22天,按0.004 2 kg/kg体重的剂量,直接投喂对虾白斑综合征病毒(WSSV)病料,并记录累积死亡率。结果表明,美人鱼发光杆菌免疫实验组对凡纳滨对虾血清中PO、ACP、AKP、UL和SOD活性影响明显高于对照组,并且在饲料中添加美人鱼发光杆菌后,明显提高了对虾抵御WSSV感染的能力。其中0.1%美人鱼发光杆菌活菌实验组的抗病毒感染能力最强,WSSV感染14d内累计死亡率为63.3%±5.8%;而对照组为96.7%±3.3%。研究表明,美人鱼发光杆菌添加在对虾饲料中能提高凡纳滨对虾非特异性免疫水平,增强抵抗疾病的能力,将其作为对虾免疫增强剂具有良好的应用前景。     

Effects of Spirulina platensis as a feed additive on growth and coloration of blue dolphin cichlids (Cyrtocara moorii Boulunger, 1902)

This study was carried out to investigate the effects of replacing fish meal with dietary Spirulina as a feed supplement on the growth performance and coloration of blue dolphin cichlids (Cyrtocara moorii). Five isonitrogenous (47% crude protein) and isocaloric (17.36 kJ/g digestible energy) diets were for formulated to replace FM with 0, 5, 10, 15 and 20% Spirulina (designated as Control, SP5, SP10, SP15 and SP20 respectively) and fed to the fish (initial body weight, 3.15 ± 0.01 g). Fish were randomly distributed into fifteen 120 L aquariums (26.5 ± 1.00°C), 15 fish per aquarium. The diets were tested in triplicate for 12 weeks. Experimental groups were fed twice daily (09:00 and 17:00) by hand to satiation. At the end of the feeding trial, significantly (p < 0.05) higher weight gain (WG), specific growth rate (SGR), protein efficiency ratio (PER) and lower feed conversion ratio (FCR) were observed in fish fed the SP10 diet when compared to the SP20 diet. There was no significant difference in these parameters between the other groups. The skin coloration of blue dolphin cichlid fed a diet containing Spirulina meal was enhanced. The best coloration was observed in the SP15 group. These impressions were objectively validated by chemical determinations of carotenoids extracted from fish skins and passed statistical tests of significance. The study findings show that Spirulina meal does not diminish growth rates except at very high levels.     

A Minchinia mercenariae‐like parasite infects cockles Cerastoderma edule in Galicia (NW Spain)

The cockle Cerastoderma edule fishery has traditionally been the most important shellfish species in terms of biomass in Galicia (NW Spain). In the course of a survey of the histopathological conditions affecting this species in the Ria of Arousa, a haplosporidan parasite that had not been observed in Galicia was detected in one of the most productive cockle beds of Galicia. Uni‐ and binucleate cells and multinucleate plasmodia were observed in the connective tissue mainly in the digestive area, gills and gonad. The parasite showed low prevalence, and it was not associated with abnormal cockle mortality. Molecular identification showed that this parasite was closely related to the haplosporidan Minchinia mercenariae that had been reported infecting hard clams Mercenaria mercenaria from the Atlantic coast of the United States. The molecular characterization of its SSU rDNA region allowed obtaining a fragment of 1,796 bp showing 98% homology with M. mercenariae parasite. Phylogenetic analysis supported this identification as this parasite was clustered in the same clade as M. mercenariae from the United States and other M. mercenariae‐like sequences from the UK, with bootstrap value of 99%. The occurrence of M. mercenariae‐like parasites infecting molluscs outside the United States is confirmed.     

Sea bream culture in Egypt; status, constraints and potential

Marine fish farming in Egypt began in 1976 with the culture of gilthead sea bream, (Sparus aurata) as this fish was notably adaptable to brackish and marine pond conditions. Today, marine fish and shrimp farms amount to about 19,000 ha, out of which 42% is already in production while the rest, i.e., 58% is still under construction. In 1997, cultured gilthead sea bream production of 2,250 tons made up 3% of the 75,000 tons total aquaculture catch. In polyculture, usually with the grey mullet and sea bass, gilthead sea bream contributed 440 kg ha^–1 to the total yield of 1,700 kg ha^–1 (26%) over a period of 16 months. For the same period, the yield of monoculture ponds averaged 100 kg ha^–1, while in marine cages, yields ranged from 4–10 kg m³. In 1996–1997, fry of 0.25–1 g and fingerlings 1–10 g with a total of 3 million, were collected from the wild and 1 million fry were produced in the three marine hatcheries out of the four existing ones. The development of sea bream culture in Egypt is now severely inhibited by the shortage of seeds and adequate feeds. Exports of both sea bream and sea bass, during 1994–1996 averaged 1,300 tons per year.     

Dietary thiamin and pyridoxine requirements of fingerling Indian major carp,Cirrhinus mrigala (Hamilton)

Two experiments were conducted to quantify the dietary thiamin (experiment I) and pyridoxine (experiment II) requirements of fingerling Cirrhinus mrigala for 16 weeks. In experiment I, dietary thiamin requirement was determined by feeding seven casein–gelatin‐based diets (400 g kg^?1 CP; 18.69 kJ g^?1 GE) with graded levels of thiamin (0, 0.5, 1, 2, 4, 8 and 16 mg kg^?1 diet) to triplicate groups of fish (6.15 ± 0.37 cm; 1.89 ± 0.12 g). Fish fed diet with 2 mg kg^?1 thiamin had highest specific growth rate (SGR), protein retention (PR), RNA/DNA ratio, haemoglobin (Hb), haematocrit (Hct), RBCs and best feed conversion ratio (FCR). However, highest liver thiamin concentration was recorded in fish fed 4 mg thiamin kg^?1 diet. Broken‐line analysis of SGR, PR and liver thiamin concentrations exhibited the thiamin requirement in the range of 1.79–3.34 mg kg^?1 diet (0.096–0.179 μg thiamin kJ^?1 gross energy). In experiment II, six casein–gelatin‐based diets (400 g kg^?1 CP; 18.69 kJ g^?1 GE) containing graded levels of pyridoxine (0, 2, 4, 6, 8 and 10 mg kg^?1 diet) were fed to triplicate groups of fish (6.35 ± 0.37 cm; 1.97 ± 0.12 g). Fish fed diet containing 6 mg kg^?1 pyridoxine showed best SGR, FCR, PR, RNA/DNA ratio, Hb, Hct and RBCs, whereas maximum liver pyridoxine concentration was recorded in fish fed 8 mg kg^?1 dietary pyridoxine. Broken‐line analysis of SGR, PR and liver pyridoxine concentrations reflected the pyridoxine requirement from 5.63 to 8.61 mg kg^?1 diet. Data generated during this study would be useful in formulating thiamin‐ and pyridoxine‐balanced feeds for the intensive culture of this fish.     

Fertilization by refrigerator stored sperm of the Indian major carp, Labeo calbasu (Hamilton, 1822)

This study reports on the spermatological properties, and on the development of a protocol for refrigerator storage (4°C) of Labeo calbasu (Hamilton, 1822) sperm for artificial breeding. Volume, motility, concentration and pH of the freshly collected sperm were 2.21 ± 0.53 (μL g^?1 of fish weight) (mean ± SD), 95 ± 1 (%), 1.93 ± 0.44 × 10⁹ (cells mL^?1) and 7.56 ± 0.17 respectively. Sperm activation was evaluated at different osmolalities of NaCl solution, and motility ceased completely when osmolality of the extender was ≥287 mOsmol kg^?1. Sperm retained motility for 24, 72 and 108 h, after refrigerator storage when sperm were undiluted, suspended in Alsever's solution and suspended in Alsever's solution containing 5% methanol respectively. Fertilization rate of fresh eggs with sperm stored at 4°C in Alsever's solution and Alsever's solution containing 5% methanol was 77% and 60% with a hatching rate of 60% and 43% respectively. The fertilization and hatching success of the stored sperm suggests potential to use refrigeration for transporting genetic material to hatcheries for artificial breeding of L. calbasu in Bangladesh.     

A novel approach to denitrification processes in a zero-discharge recirculating system for small-scale urban aquaculture

This paper presents an innovative process to solve the nitrate build-up problem in recirculating aquaculture systems (RAS). The novel aspects of the process lie in a denitrification bioreactor system that uses solid cotton wool as the primary carbon source and a unique degassing chamber. In the latter, the water is physically stripped of dissolved gaseous O₂ (by means of a Venturi vacuum tube), and the subsequent denitrification becomes more efficient due to elimination of the problems of oxygen inhibition of denitrification and aerobic consumption of cotton wool. The cotton wool medium also serves as a physical barrier that traps organic particles, which, in turn, act as an additional carbon source for denitrification. Operation in the proposed system gives an extremely low C/N ratio of 0.82 g of cotton wool/g of nitrate N, which contributes to a significant reduction of biofilter volume. The additional advantage of using solid cotton wool as the carbon source is that it does not release organic residuals into the liquid to be recycled. Operation of the system over a long period consistently produced effluents with low nitrate levels (below 10 mg N/l), and there was only a very small need to replace system water. The overall treatment scheme, also incorporating an aerobic nitrification biofilter and a granular filtration device, produced water of excellent quality, i.e., with near-zero levels of nitrite and ammonia, a sufficiently high pH for aquaculture, and low turbidity. The proposed system thus provides a solution for sustainable small-scale, urban aquaculture operation with a very high recovery of water (over 99%) and minimal waste disposal.     

Effects of metomidate anaesthesia or transfer to pur sea water on plasma parameters in ammonia-exposed Atlantic salmon (Salmo salar L) in sea water

Atlantic salmon (Salmo salar L) postsmolts weighing 150 ± 53 g were exposed to 14–15 mg l^–1 TA-N (total ammonia-N) in sea water in 1 m³ tanks for 24h. Blood samples were then taken A) immediately after the fish were netted from the exposure tanks and stunned by a blow to the head; B) 2–20 min after the fish were transferred to 15 l of an anaesthetic solution of metomidate in ammonia-free sea water; or C) 2–20 min after the fish were transferred to 15 l of ammonia-free sea water. Plasma TA-N level was 18% lower in the anaesthetised fish compared to in the fish sampled directly from the exposure tanks (p 0.05), and accordingly 16% lower in the fish transferred to pure sea water although this difference was not significant (p = 0.07). Plasma glucose level was higher in the fish transferred to pure sea water than in the fish receiving the two other treatments (p 0.05), but plasma urea, osmolality, Na^{+, Cl–, Ca2+ or Mg2+} levels did not vary significantly between the different treatments. Plasma TA-N level increased with time in the fish in the metomidate solution (p 0.02).