高坝鱼道工程设计案例分析

坝高１１６ｍ,最大水头６７.０ｍ,额定水头５３．５ｍ。主要过鱼季节为３－６月,兼顾过鱼季节为２月、７－１０月。综合考虑工程特性和过鱼对象生态习性,设计采用竖缝式池室结构型式鱼道,主要由进口段、槽身段、暗涵段、明渠段、过坝段、出口段、进口补水设施和观测研究室等部分组成,全长３５６６．３２５ｍ。共设置２个常用进口,２个备用进口,鱼道过鱼孔设计流速１．１ｍ／ｓ。介绍了鱼道设计技术路线及研究方法,提出了休息池、鱼道进口、补水设施和带集鱼池的观测研究室的设计思路。     

连江西牛鱼道运行效果的初步研究

鱼道建设被认为是有效缓解水坝阻隔鱼类洄游的重要方法之一,而鱼道效果监测是评价其功能的重要环节。连江西牛鱼道是广东省第一座建立在水坝主体上的过鱼通道,为了解其运行效果,采用张网法和截堵法,２０１２年３－８月共６次对西牛鱼道的过鱼效果进行监测。研究表明,西牛鱼道共监测到鱼类３目、８科、３０属、３８种,以银鮈（Squalidus argentatus）、乐山小鳔鮈（Microphysogobio kiatingensis）、子陵吻鰕虎（Rhinogobit giurinus）等小型鱼类为优势类群。进入鱼道的鱼类呈现昼夜差异,上午集鱼效果要显著优于其他时间段;不同季节集鱼的种类和数量呈现较大差别,３－８月集鱼数量逐渐减少,集鱼种类数以５月最多。与国内外其他鱼道的过鱼效果比较,西牛鱼道能较好地发挥其功能。     

亚马逊流域玛代拉河Santo Antonio 鱼道设计与建设的启示

Santo Antonio过鱼设施位于亚马逊河最大支流玛代拉河下游,其设计规模宏大,在世界范围内鲜有,并且工程建设地所在区域水文环境和鱼类生物学特征等与我国长江较为类似,通过分析该过鱼设施,得出启示主要包括：（1）过鱼设施是为生物对象设计的水利工程,其设计和建设过程中必须由工程师和生物学家紧密合作;（2）吸引水流对过鱼设施的成败起关键性作用,虽然吸引水流系统需要加大投资并且运行过程中会损失大量的水能,但从确保过鱼设施效果而言是非常必要的;（3）物理模型对于鱼道进口位置,鱼道内部水力学特征的确定等具有重要参考价值,尤其在1:1局部模型内开展的生物学实验是细化和优化鱼道内部结构细节的有效方法;（4）卵石框(石笼）隔板的水池分隔方法兼具水池式鱼道与仿自然通道的双重特征,并且完工后还可以根据鱼道的运行情况灵活调整,非常方便;（5）进口处采用较高的人字闸门,即可适应尾水变化,又可与吸引水流系统相配合,创造出有效的吸引水流,一举多得;（6）全部采用大功率灯泡式水轮机是保护下行鱼类的有效措施;（7）监测系统和公众教育等配套设施的建设非常必要,有利于改进鱼道运行方式和提高过鱼效率。     

峡江鱼道过鱼效果初步评价

鱼道是解决拦河筑坝阻隔鱼类洄游的重要手段之一。为了满足兴建峡江水利枢纽工程后洄游鱼类上溯的要求,保护赣江水生生态环境的完整性,减缓工程对鱼类种群遗传交流的影响,针对峡江水利枢纽实际情况制定了鱼道过鱼方式。鱼道采用垂直竖缝式结构设计,由上游鱼道(出口段)、坝体过鱼孔口、下游主、副鱼道(进口段)、集鱼系统及连接段组成,设计主要过鱼季节为4—7月,流速0.7～1.2 m/s。采用仪器监测与人工观察相结合的方式对峡江鱼道过鱼效果进行了初步监测。监测结果显示:2017年监测到过鱼总数67.8万尾,其中上行占41.67%;期间共监测到22种鱼类,共计4目、7科、18属,以小型鱼类为主,如贝氏■(Hemiculter bleekeri)、三角鲂(Megalobrama terminalis)、银鲴(Xenocypris argentea)、银鮈(Squalidus argentatus);不同季节过鱼数量不同,其中以二季度最多,占总数49.17%,过鱼数量以7月最多;过鱼数量昼夜差别大,以上午10:00至下午16:00是过鱼数量最多;与国内其他鱼道相比,峡江鱼道过鱼数量较多,过鱼效果显著。     

与鱼道设计相关的鱼类游泳行为研究

在鱼类生境遭受破坏，渔业资源日益减少的今天，修建鱼道等过鱼设施已成为减缓水利工程建设所致不利影响的重要举措。纵观世界各国，早期的鱼道建设往往因为缺乏对鱼类的了解而最终成为摆设。我国的鱼道建设起步晚，其再度兴起需要更可靠的生物学信息。鱼类的游泳能力及行为关系着鱼道设计的各个方面，决定着设计的成败。文章针对鱼道设计，综述了国内外几十年来鱼类游泳行为的研究成果。鱼类的游泳速度可分为三类，即持续游泳速度，耐久游泳速度和突进游泳速度，其中与鱼道设计密切相关的是后两者。影响鱼类游泳速度的因素既有生物个体因素也有环境因素，主要包括摆尾频率、体长、疲劳时间、温度及耗氧量等。文章最后简述了鱼类行为学在鱼道设计中的应用。我国针对过鱼设施建设的鱼类行为学研究极少，总结国外的研究方法和经验成果可为我国的过鱼设施建设提供生物学基础，促进该领域的发展。     

流溪河水厂坝丹尼尔式鱼道运行效果初探

为检验流溪河水厂坝建立的丹尼尔式鱼道试点工程效果,在2015-2016年鱼道主要过鱼时段4-10月,利用张网法逐日监测。结果显示,鱼道取样共采集鱼类906尾,隶属于3目、8科、32属、34种,体长范围5～74cm,优势种为尼罗罗非鱼(Tilapia niloticus)(数量比51.10%)、歺又鱼(Hemiculter leucisculus)(16.23%)、唇鱼骨(Hemibarbus labeo)(8.17%)、银鮈(Squalidus argentatus)(6.73%)、东方墨头鱼(Garra orientalis)(1.55%),以定居型鱼类为主,未见河口洄游性鱼类。水温与上游水位是影响鱼类种类时间分布的主要环境因素。聚类分析表明,6月过鱼群体的种类和数量独成一类,与其他月份鱼类群体最不相似。虽然Shannon多样性指数在5月和6月分别为2.068和1.719,但总体偏低(1.451±0.535),而Margalef丰富度指数差别较小,表明不同鱼类对鱼道入口的感应存在种间差异,推测是由工程全段翻滚闸门下泄水流导致鱼道入口不易发现所致。研究表明,丹尼尔式鱼道试点工程效果较好,为该河段的绝大部分鱼类提供了上溯通道,对流溪河构建整体过鱼设施体系起到了良好的示范作用,有助于流溪河生态系统的健康与恢复。     

浅议我国鱼道运行管理存在的问题及对策-以洣水洋塘鱼道为例

鱼道作为能够有效联通闸、坝上下游水流,为鱼类提供上溯、下行通道的有效途径,一直以来被作为水利水电工程建设鱼类保护的重要措施采用,但由于认识、经费、运行管理等多方面因素,其过鱼效果及发挥的作用难以被肯定。本文在综述国内外鱼道运行管理体制现状基础上,以洣水洋塘鱼道为例,分析总结了该鱼道兴衰历程,提出我国鱼道运行管理应提升对过鱼设施建设必要性的认知度、捋顺管理机制体制、建立生态补偿机制的对策与建议,力求能够为鱼道这项鱼类保护措施的建设与发展提供支持。     

日本阶梯型鱼道模型放鱼(香鱼)试验 日本渔业工程研究和建设系列报道(一)

一、序在以治水或兴利为目的修建水工建筑物中,为保护水产资源,必须同时设置各种渔业工程。例如:为防止鱼类迷入坝和堰的取水口而建的拦鱼工程,为鱼类能够洄游而建的鱼道,或者为鱼类提供避难场而设置的鱼巢等。日本在东北部北海道设置的鱼道,大多数以通过鲑、鳟为主,而其他地区设置的鱼道大多以通过香鱼(Ayu,Japanese tzout,Plecoglssusaltivelis)为主.由于日本的鱼道设计以欧美的理论为模式,因而无论哪种鱼道,都不能充分地发挥作用。     

“一种适用于低水头水坝的过鱼通道加建方法和过鱼通道”获发明专利授权并获推广应用

<正>由中国水产科学研究院珠江水产研究所渔业资源生态研究室李新辉研究员等发明的"一种适用于低水头水坝的过鱼通道加建方法和过鱼通道"获得国家发明专利授权。该发明专利公开了一种在已建的低水头水坝加建过鱼通道的方法及加建方式,该方法解决了小型水坝造成的鱼类洄     

关于葛洲坝工程不必附建过鱼设施的意见

葛洲坝水利枢纽是在长江干流上兴建的第一个大坝工程。这个坝在湖北省宜昌市附近江面上拦断了滚滚江水,那里的部分鱼类将不能象原来那样上下游动。这一情况是否影响长江的整个鱼类资源?哪些鱼类可能受到影响?影响程度怎样?是否要给某种鱼类设置鱼道，即“过鱼设施”?有鱼道是否就能过鱼?除了鱼道外，还有没有其他保护鱼类资源的措施和方法?这一系列问题，引起了人们的极大关注。     

Association between the interannual variation in the oceanic environment and catch rates of bigeye tuna (Thunnus obesus) in the Atlantic Ocean

The environmental processes associated with variability in the catch rates of bigeye tuna in the Atlantic Ocean are largely unexplored. This study used generalized additive models (GAMs) fitted to Taiwanese longline fishery data from 1990 to 2009 and investigated the association between environmental variables and catch rates to identify the processes influencing bigeye tuna distribution in the Atlantic Ocean. The present findings reveal that the year (temporal factor), latitude and longitude (spatial factors), and major regular longline target species of albacore catches are significant for the standardization of bigeye tuna catch rates in the Atlantic Ocean. The standardized catch rates and distribution of bigeye tuna were found to be related to environmental and climatic variation. The model selection processes showed that the selected GAMs explained 70% of the cumulative deviance in the entire Atlantic Ocean. Regarding environmental factors, the depth of the 20 degree isotherm (D20) substantially contributed to the explained deviance; other important factors were sea surface temperature (SST) and sea surface height deviation (SSHD). The potential fishing grounds were observed with SSTs of 22–28°C, a D20 shallower than 150 m and negative SSHDs in the Atlantic Ocean. The higher predicted catch rates were increased in the positive northern tropical Atlantic and negative North Atlantic Oscillation events with a higher SST and shallow D20, suggesting that climatic oscillations affect the population abundance and distribution of bigeye tuna.     

Protein and amino acid composition from the mantle of juvenile O ctopus vulgaris exposed to prolonged starvation

Protein and amino acid composition of the mantle of juvenile O ctopus vulgaris (Cuvier, 1797) during fasting for 27 days were determined. Average protein content of octopus mantle was of 711.19 ± 46.80 g kg^?1 DW, and it decreased with increasing fasting days. The non‐essential amino acids content was higher (486.18 ± 11.08 g kg^?1 protein) than essential amino acids (425.82 ± 9.15 g kg^?1 protein) at the start of the experiment (unstarved animals). The results suggest that the amino acid profile of the mantle where the most abundant amino acids are Arg, His, Lys, Gly, Leu and Pro could indicate a prolonged fasting condition (>20 days) or poor nutrition of O . vulgaris. This study supports the idea of using mantle for metabolic needs of starved O . vulgaris suggesting that the degradation pathway of amino acids to pyruvate and tricarboxylic acid cycle intermediates was favoured contrary to the degradation pathway of ketogenic amino acids. Special considerations should be taken concerning Thr, Ile, Ser, Ala, Asx (Asp, Asn), Glx (Glu, Gln) (because of their fast intake) and Lys and His (due to their stable contents) during a prolonged period of fasting.     

Growth performance,digestive enzyme activity and immune response of Japanese sea bass,Lateolabrax japonicus fed with fructooligosaccharide

In this experiment, a feeding trial was performed to determine the effects of fructooligosaccharide (FOS) on growth performance, digestive enzyme activity and immune response of Japanese sea bass, Lateolabrax japonicus juveniles (initial weight 38.3 ± 0.5 g), and the fish were examined following feeding with six levels of FOS (0, 0.5, 1, 2, 4 and 6 g/kg) for 28 days. Significant enhancement of weight gain (WG) and specific growth rate (SGR) was found in fish fed 1 g/kg FOS incorporated diets (p < .05), while the feed conversion ratio (FCR) in the 1, 2 g/kg FOS groups reduced significantly compared with the control (p < .05). Besides, the crude lipid in the 4, 6 g/kg FOS groups increased significantly compared with the control (p < .05). On the other hand, the erepsin and lipase activities significantly elevated in intestine of fish fed 2 g/kg FOS (p < .05) and the lysozyme activity in serum of fish fed 2 g/kg FOS were significantly higher than that in the control (p < .05). Moreover, the alkaline phosphatase activities in serum of fish fed 0.5, 1, 2 g/kg FOS were significantly higher than in control (p < .05). Regression analysis showed that the relationships between dietary FOS levels and either SGR, FCR, erepsin or lysozyme activities were best expressed by regression equations, and the optimal inclusion levels are 1.37, 1.80, 3.06, 3.11, 1.93 and 1.80 g/kg for SGR, FCR, erepsin, lipase, lysozyme and total superoxide dismutase activities, respectively. Overall, this study revealed that FOS incorporated diets could beneficial for L. japonicus culture in terms of increasing the growth, digestion and immune activities. Under the present experimental condition, the optimal supplementary level of FOS in the diet of L. japonicus is 1–3 g/kg.     

美人鱼发光杆菌对凡纳滨对虾非特异性 免疫功能及抗病力的影响

在基础饲料中分别添加0.1%和1%美人鱼发光杆菌灭活菌、0.1%美人鱼发光杆菌活菌配制成3种免疫实验饲料,以基础饲料为空白对照组饲料,每组设3个平行样。对个体质量为(4.83±0.36)g的凡纳滨对虾进行为期20 d的饲养实验,分别在0、5、10、15和20d进行取样,以血清中的酚氧化酶(PO)、酸性磷酸酶(ACP)、碱性磷酸酶(AKP)、超氧化物歧化酶(SOD)和溶菌酶(UL)活性为免疫指标,探讨了美人鱼发光杆菌作为免疫制剂对凡纳滨对虾非特异性免疫效应的影响;在投喂免疫饲料后的第22天,按0.004 2 kg/kg体重的剂量,直接投喂对虾白斑综合征病毒(WSSV)病料,并记录累积死亡率。结果表明,美人鱼发光杆菌免疫实验组对凡纳滨对虾血清中PO、ACP、AKP、UL和SOD活性影响明显高于对照组,并且在饲料中添加美人鱼发光杆菌后,明显提高了对虾抵御WSSV感染的能力。其中0.1%美人鱼发光杆菌活菌实验组的抗病毒感染能力最强,WSSV感染14d内累计死亡率为63.3%±5.8%;而对照组为96.7%±3.3%。研究表明,美人鱼发光杆菌添加在对虾饲料中能提高凡纳滨对虾非特异性免疫水平,增强抵抗疾病的能力,将其作为对虾免疫增强剂具有良好的应用前景。     

Effects of Spirulina platensis as a feed additive on growth and coloration of blue dolphin cichlids (Cyrtocara moorii Boulunger, 1902)

This study was carried out to investigate the effects of replacing fish meal with dietary Spirulina as a feed supplement on the growth performance and coloration of blue dolphin cichlids (Cyrtocara moorii). Five isonitrogenous (47% crude protein) and isocaloric (17.36 kJ/g digestible energy) diets were for formulated to replace FM with 0, 5, 10, 15 and 20% Spirulina (designated as Control, SP5, SP10, SP15 and SP20 respectively) and fed to the fish (initial body weight, 3.15 ± 0.01 g). Fish were randomly distributed into fifteen 120 L aquariums (26.5 ± 1.00°C), 15 fish per aquarium. The diets were tested in triplicate for 12 weeks. Experimental groups were fed twice daily (09:00 and 17:00) by hand to satiation. At the end of the feeding trial, significantly (p < 0.05) higher weight gain (WG), specific growth rate (SGR), protein efficiency ratio (PER) and lower feed conversion ratio (FCR) were observed in fish fed the SP10 diet when compared to the SP20 diet. There was no significant difference in these parameters between the other groups. The skin coloration of blue dolphin cichlid fed a diet containing Spirulina meal was enhanced. The best coloration was observed in the SP15 group. These impressions were objectively validated by chemical determinations of carotenoids extracted from fish skins and passed statistical tests of significance. The study findings show that Spirulina meal does not diminish growth rates except at very high levels.     

Dietary thiamin and pyridoxine requirements of fingerling Indian major carp,Cirrhinus mrigala (Hamilton)

Two experiments were conducted to quantify the dietary thiamin (experiment I) and pyridoxine (experiment II) requirements of fingerling Cirrhinus mrigala for 16 weeks. In experiment I, dietary thiamin requirement was determined by feeding seven casein–gelatin‐based diets (400 g kg^?1 CP; 18.69 kJ g^?1 GE) with graded levels of thiamin (0, 0.5, 1, 2, 4, 8 and 16 mg kg^?1 diet) to triplicate groups of fish (6.15 ± 0.37 cm; 1.89 ± 0.12 g). Fish fed diet with 2 mg kg^?1 thiamin had highest specific growth rate (SGR), protein retention (PR), RNA/DNA ratio, haemoglobin (Hb), haematocrit (Hct), RBCs and best feed conversion ratio (FCR). However, highest liver thiamin concentration was recorded in fish fed 4 mg thiamin kg^?1 diet. Broken‐line analysis of SGR, PR and liver thiamin concentrations exhibited the thiamin requirement in the range of 1.79–3.34 mg kg^?1 diet (0.096–0.179 μg thiamin kJ^?1 gross energy). In experiment II, six casein–gelatin‐based diets (400 g kg^?1 CP; 18.69 kJ g^?1 GE) containing graded levels of pyridoxine (0, 2, 4, 6, 8 and 10 mg kg^?1 diet) were fed to triplicate groups of fish (6.35 ± 0.37 cm; 1.97 ± 0.12 g). Fish fed diet containing 6 mg kg^?1 pyridoxine showed best SGR, FCR, PR, RNA/DNA ratio, Hb, Hct and RBCs, whereas maximum liver pyridoxine concentration was recorded in fish fed 8 mg kg^?1 dietary pyridoxine. Broken‐line analysis of SGR, PR and liver pyridoxine concentrations reflected the pyridoxine requirement from 5.63 to 8.61 mg kg^?1 diet. Data generated during this study would be useful in formulating thiamin‐ and pyridoxine‐balanced feeds for the intensive culture of this fish.     

Sea bream culture in Egypt; status, constraints and potential

Marine fish farming in Egypt began in 1976 with the culture of gilthead sea bream, (Sparus aurata) as this fish was notably adaptable to brackish and marine pond conditions. Today, marine fish and shrimp farms amount to about 19,000 ha, out of which 42% is already in production while the rest, i.e., 58% is still under construction. In 1997, cultured gilthead sea bream production of 2,250 tons made up 3% of the 75,000 tons total aquaculture catch. In polyculture, usually with the grey mullet and sea bass, gilthead sea bream contributed 440 kg ha^–1 to the total yield of 1,700 kg ha^–1 (26%) over a period of 16 months. For the same period, the yield of monoculture ponds averaged 100 kg ha^–1, while in marine cages, yields ranged from 4–10 kg m³. In 1996–1997, fry of 0.25–1 g and fingerlings 1–10 g with a total of 3 million, were collected from the wild and 1 million fry were produced in the three marine hatcheries out of the four existing ones. The development of sea bream culture in Egypt is now severely inhibited by the shortage of seeds and adequate feeds. Exports of both sea bream and sea bass, during 1994–1996 averaged 1,300 tons per year.     

A Minchinia mercenariae‐like parasite infects cockles Cerastoderma edule in Galicia (NW Spain)

The cockle Cerastoderma edule fishery has traditionally been the most important shellfish species in terms of biomass in Galicia (NW Spain). In the course of a survey of the histopathological conditions affecting this species in the Ria of Arousa, a haplosporidan parasite that had not been observed in Galicia was detected in one of the most productive cockle beds of Galicia. Uni‐ and binucleate cells and multinucleate plasmodia were observed in the connective tissue mainly in the digestive area, gills and gonad. The parasite showed low prevalence, and it was not associated with abnormal cockle mortality. Molecular identification showed that this parasite was closely related to the haplosporidan Minchinia mercenariae that had been reported infecting hard clams Mercenaria mercenaria from the Atlantic coast of the United States. The molecular characterization of its SSU rDNA region allowed obtaining a fragment of 1,796 bp showing 98% homology with M. mercenariae parasite. Phylogenetic analysis supported this identification as this parasite was clustered in the same clade as M. mercenariae from the United States and other M. mercenariae‐like sequences from the UK, with bootstrap value of 99%. The occurrence of M. mercenariae‐like parasites infecting molluscs outside the United States is confirmed.     

Involvement of gonadal steroids in final oocyte maturation of white perch (Morone americana) and white bass (M. chrysops): in vivo and in vitro studies

Plasma estradiol-17 (E₂), testosterone (T), 17,20-dihydroxy-4-pregnen-3-one (DHP) and 17,20,21-tri-hydroxy-4-pregnen-3-one (20-S) levels were measured by radioimmunoassay (RIA) in white perch (Morone americana) and white bass (M. chrysops) that were induced to undergo final oocyte maturation (FOM) with human chorionic gonadotropin (hCG). Plasma DHP levels increased in females of both species in association with oocyte germinal vesicle migration (GVM) and germinal vesicle breakdown (GVBD) and decreased thereafter. Plasma 20-S levels also increased with oocyte GVM in white bass, but were several-fold lower than DHP levels. Circulating E₂ and T levels were greatest during GVM and GVBD in both species and decreased to low levels during oocyte hydration and ovulation. Follicles from white perch and white bass which received a priming injection of hCG in vivo, produced both DHP and 20-S in vitro after exposure to hCG and their oocytes underwent GVBD. Ovarian incubates from unprimed fish of either species produced only E₂ and T and their oocytes did not complete GVBD. Oocytes from unprimed bass, but not perch, matured when follicles were exposed to hCG in vitro. Both trilostane and cycloheximide blocked in vitro production of DHP and 20-S and oocyte GVBD by white perch follices. DHP and 20-S were equipotent inducers of FOM in the GVBD bioassay. None of several other structurally-related steroids tested were effective within a physiological range of concentrations. These results indicate a role for DHP and 20-S in the control of FOM in white perch and white bass.