白令海阿留申海盆区狭鳕当年生幼鱼数量分布的调查研究

１９９３年夏季白令海狭鳕资源调查期间，作者等应用声学／拖网评估调查方法，首次发现阿留申海盆区有大量当年生狭鳕幼鱼分布。研究结果表明：（１）当年生幼鱼主要分布在海盆区东北部，密集区位于东白令海陆架斜坡与公海区之间，呈西北东南走向；（２）鱼群主要栖息于中上层８０—１２０米处，有明显的昼夜垂直移动现象；（３）７月份海盆区东北部幼鱼鱼体长度为２９—４７毫米，而南部波哥斯洛夫岛附近的鱼体长度仅为１１—１７毫米，系两个不同产卵期鱼群的子代；（４）幼鱼主要栖息层的水温为３—５℃，盐度为３２．９—３３．１，其分布与陆架斜坡地理环境和饵料生物关系较密切。     

白令海阿留申海盆区夏季理化环境特征及其与狭鳕分布和移动的关系

１９９３年６～８月，“北斗”号渔业资源调查船在白令海阿留申海盆区进行了狭鳕资源声学调查及渔场环境调查。应用本航次调查资料，本文阐述了调查海区温度、盐度及溶解氧分布特征，并分析了狭鳕分布和移动与理化环境的关系。结果表明：（１）阿留申海盆区为大面积低温水所覆盖，水温在１０℃以下。温盐水平分布特征为公海区低温高盐，调查区的其余水域则为高温低盐。溶解氧水平分布比较均匀，表层溶氧含量在１０ｍｇ／Ｌ左右；（２）温跃层位于２５米和５０米之间，一强冷水团控制着１００米到２００米左右的水层，其水温在３℃以下。盐度随深度增加而增加，溶解氧随深度的增加而降低；（３）狭鳕当年生幼鱼主要分布在海盆水系和东大陆架水系的混合区内，１００米层幼鱼密集区水温在３～５℃，盐度在３２．９～３３．１，溶解氧在８～９ｍｇ／Ｌ；（４）夏季狭鳕成鱼主要在冷水团范围以内垂直移动，２００米层成鱼密集区水温为３～４℃，盐度为３３．２～３３．６，溶解氧为６～８ｍｇ／Ｌ。     

狭鳕（Theragra chalcogramma Pallas）目标强度的现场测定↑（*）

１９９３年夏季“北斗”号调查船白令海狭鳕资源声学调查期间，利用计数－积分法对狭鳕的目标强度进行了现场测定。结果表明，平均叉长５１．６ｃｍ的狭鳕的目标强度为－３４．３６ｄＢ；对常规的２０Ｌｏｇｌ形式表示，其目标强度与叉长的关系式为ＴＳ＝２０Ｌｏｇｌ－６８．６ｄＢ。     

白令海公海渔业条约第11次年会9月5日在波兰华沙召开

自上世纪80年代中期起日本、韩国、波兰、中国的大型远洋拖网船先后进入白令海中部公海捕捞狭鳕后，白令海公海的狭鳕渔获量迅速增加，1989年猛增至140．7万t（创历史最高记录），作业的拖网船数为188艘左右，当时白令海公海的狭鳕资源量也处于高水平为200万t左右。     

狭鳕（Theragra chalcogramma Palas）目标强度的现场测定

１９９３年夏季“北斗”号调查船白令海狭鳕（ＴｈｅｒａｇｒａｃｈａｌｃｏｇｒａｍｍａＰａｌａｓ）资源声学调查期间，利用计数—积分法对狭鳕的目标强度进行了现场测定。结果表明，平均叉长５１６ｃｍ的狭鳕的平均目标强度为－３４３ｄＢ；以常规的２０Ｌｏｇｌ形式表示，其目标强度与叉长的关系式为ＴＳ＝２０Ｌｏｇｌ－６８．６ｄＢ。该测定结果较目前采用的关系式：ＴＳ＝２０Ｌｏｇｌ－６６．０ｄＢ低２６ｄＢ；应用于资源量计算，其估算值将比现估算值高８２％。     

日本在白令海进行狭鳕人工放流

1984年世界狭鳕产量从1980年的402万吨增至599万吨，居各鱼种之首，是一种很有希望的鱼种，其分布主要在北部太平洋的美、苏200海里水域内。近年来，美、苏两国给日本渔船的狭鳕捕捞配额逐年减少，1985年以后日本的拖网渔船不得不逐渐移转到白令海公海去捕捞狭鳕。因此，近两年来白令海公海的狭鳕渔场已成为日本拖网渔船的主要作业渔场。日本为了增加该公海的狭鳕资源达到其拖网渔船的稳定经营为目的。     

据美国NOAA调查船调查东白令海的狭鳕主群向北移动明显

2007年6～7月的夏季期间，美国NOAA（海洋大气厅）调查船Oscatrdison（音译）号在东白令海从事的狭鳕资源调查中，发现该水域的狭鳕资源状态良好，特别是与2006年度的调查相比，出现了数量相当的狭鳕幼鱼，这是资源健全的迹象。然而，与之同时也发现了该水域的狭鳕分布状况有了很明显的变化。以往狭鳕的主要作业渔场位于阿留申群岛东侧的乌纳拉斯卡岛东北外海的普里比洛夫群岛的一带水域，而今年呢这个主要渔场的狭鳕数量却很少，而在普里比洛夫群岛西北外海到靠近俄罗斯专属区线的一带水域却出现了密集的狭鳕鱼群，显然东白令海的狭鳕主群从普里比洛夫群岛水域向北移动了，这是否与地球的变暖有关呢？有待研究。[第一段]     

日本开始调查白令公海狭鳕资源

日本水产厅为摸清阿留申海盆水域中的狭鳕资源量及种群群系,于1988年12月1日派出“开洋丸(2,644吨)”调查船前往上述海域进行调查。近年来,白令公海已成为日本、南朝鲜、波兰等远洋渔业国极为重要的狭鳕渔场。本次日本水产厅决定调查白令公海中的阿     

夏季白令海温跃层和中层冷水的分布特征

研究了夏季白令海阿留中海盆区温跃层和中层冷水的分布特点及形成机制。结果表明，（１）以水温为主要分析指标，调查海区自上而下（０～５００ｍ）层，可分为上匀和层、浊跃层、中居冷水和下匀和层（２）温跃层广泛分布于大部分调查海域，绝大多数测站其深度在２５～５０ｍ，强度在０．１０～０．１６℃／ｍ，其中东南部为一相对强温跃区（≥９０．１５℃／ｍ）；（３）中层冷水的上界深度在４０～１３０ｍ左右，下界深度在１５０～     

2010年白令海公海狭鳕的捕捞继续禁捕一年

自上世纪80年代中期起日本、韩国、波兰、中国的大型拖网船先后进入自令海中部的公海捕捞狭鳕后，白令海公海狭鳕的产量迅速增加，从1985年的35万t猛增至1989年的140万t，作业的拖网船数也从1985年的105艘增至185艘，然而由于捕捞过度，致使狭鳕的产量迅速减少，从1989年的140万t剧减至1991年的29．3万t，到1992年时竟剧减到仅1万t。     

Association between the interannual variation in the oceanic environment and catch rates of bigeye tuna (Thunnus obesus) in the Atlantic Ocean

The environmental processes associated with variability in the catch rates of bigeye tuna in the Atlantic Ocean are largely unexplored. This study used generalized additive models (GAMs) fitted to Taiwanese longline fishery data from 1990 to 2009 and investigated the association between environmental variables and catch rates to identify the processes influencing bigeye tuna distribution in the Atlantic Ocean. The present findings reveal that the year (temporal factor), latitude and longitude (spatial factors), and major regular longline target species of albacore catches are significant for the standardization of bigeye tuna catch rates in the Atlantic Ocean. The standardized catch rates and distribution of bigeye tuna were found to be related to environmental and climatic variation. The model selection processes showed that the selected GAMs explained 70% of the cumulative deviance in the entire Atlantic Ocean. Regarding environmental factors, the depth of the 20 degree isotherm (D20) substantially contributed to the explained deviance; other important factors were sea surface temperature (SST) and sea surface height deviation (SSHD). The potential fishing grounds were observed with SSTs of 22–28°C, a D20 shallower than 150 m and negative SSHDs in the Atlantic Ocean. The higher predicted catch rates were increased in the positive northern tropical Atlantic and negative North Atlantic Oscillation events with a higher SST and shallow D20, suggesting that climatic oscillations affect the population abundance and distribution of bigeye tuna.     

Growth performance,digestive enzyme activity and immune response of Japanese sea bass,Lateolabrax japonicus fed with fructooligosaccharide

In this experiment, a feeding trial was performed to determine the effects of fructooligosaccharide (FOS) on growth performance, digestive enzyme activity and immune response of Japanese sea bass, Lateolabrax japonicus juveniles (initial weight 38.3 ± 0.5 g), and the fish were examined following feeding with six levels of FOS (0, 0.5, 1, 2, 4 and 6 g/kg) for 28 days. Significant enhancement of weight gain (WG) and specific growth rate (SGR) was found in fish fed 1 g/kg FOS incorporated diets (p < .05), while the feed conversion ratio (FCR) in the 1, 2 g/kg FOS groups reduced significantly compared with the control (p < .05). Besides, the crude lipid in the 4, 6 g/kg FOS groups increased significantly compared with the control (p < .05). On the other hand, the erepsin and lipase activities significantly elevated in intestine of fish fed 2 g/kg FOS (p < .05) and the lysozyme activity in serum of fish fed 2 g/kg FOS were significantly higher than that in the control (p < .05). Moreover, the alkaline phosphatase activities in serum of fish fed 0.5, 1, 2 g/kg FOS were significantly higher than in control (p < .05). Regression analysis showed that the relationships between dietary FOS levels and either SGR, FCR, erepsin or lysozyme activities were best expressed by regression equations, and the optimal inclusion levels are 1.37, 1.80, 3.06, 3.11, 1.93 and 1.80 g/kg for SGR, FCR, erepsin, lipase, lysozyme and total superoxide dismutase activities, respectively. Overall, this study revealed that FOS incorporated diets could beneficial for L. japonicus culture in terms of increasing the growth, digestion and immune activities. Under the present experimental condition, the optimal supplementary level of FOS in the diet of L. japonicus is 1–3 g/kg.     

Involvement of gonadal steroids in final oocyte maturation of white perch (Morone americana) and white bass (M. chrysops): in vivo and in vitro studies

Plasma estradiol-17 (E₂), testosterone (T), 17,20-dihydroxy-4-pregnen-3-one (DHP) and 17,20,21-tri-hydroxy-4-pregnen-3-one (20-S) levels were measured by radioimmunoassay (RIA) in white perch (Morone americana) and white bass (M. chrysops) that were induced to undergo final oocyte maturation (FOM) with human chorionic gonadotropin (hCG). Plasma DHP levels increased in females of both species in association with oocyte germinal vesicle migration (GVM) and germinal vesicle breakdown (GVBD) and decreased thereafter. Plasma 20-S levels also increased with oocyte GVM in white bass, but were several-fold lower than DHP levels. Circulating E₂ and T levels were greatest during GVM and GVBD in both species and decreased to low levels during oocyte hydration and ovulation. Follicles from white perch and white bass which received a priming injection of hCG in vivo, produced both DHP and 20-S in vitro after exposure to hCG and their oocytes underwent GVBD. Ovarian incubates from unprimed fish of either species produced only E₂ and T and their oocytes did not complete GVBD. Oocytes from unprimed bass, but not perch, matured when follicles were exposed to hCG in vitro. Both trilostane and cycloheximide blocked in vitro production of DHP and 20-S and oocyte GVBD by white perch follices. DHP and 20-S were equipotent inducers of FOM in the GVBD bioassay. None of several other structurally-related steroids tested were effective within a physiological range of concentrations. These results indicate a role for DHP and 20-S in the control of FOM in white perch and white bass.     

Cardiac,ventilatory and metabolic responses of two ecologically dissimilar species of fish to waterborne cyanide

Changes in heart rate, ventilatory activity and oxygen consumption were determined in trout (Salmo gairdneri) and brown bullhead catfish (Ictalurus nebulosus) during exposure to a steadily increasing concentration of waterborne cyanide selected to produce death in 8–9 hours for each species. The lethal cyanide concentration for the bullheads was an order of magnitude higher than for trout. Trout developed an immediate and gradually increasing bradycardia throughout the exposure period. Cyanide produced tachycardia in the bullhead followed by a gradual onset of bradycardia as the concentration of cyanide was raised. Pericardial injection of atropine (a muscarinic cholinergic antagonist) indicated that bradycardia in the trout was due initially to increased vagal tone but later due to the direct effect of cyanide on the heart. Hyperventilation in the trout persisted throughout the exposure period, although the rate and amplitude fluctuated and was variable between individual fish. During the last hour of exposure (highest cyanide concentration), ventilation was characterized by rapid, shallow breaths followed by a sudden respiratory arrest. The bullheads exhibited hyperventilation during the first 3 hours of exposure followed by a gradual, linear drop in ventilation rate and amplitude until death occurred. Cardiac and ventilatory responses in both species were attributed to stimulation of central and peripheral chemoreceptors by cyanide. Evidence is presented which suggests the initial response in the bullheads was due, at least in part, to gustatory stimulation by the cyanide. Oxygen consumption of the trout remained above pre-exposure levels for the majority of the test period. Oxygen consumption in the bullhead paralleled the changes in heart and ventilatory rates. Whole-body lactate levels of fingerlings of both species during cyanide exposure were measured to estimate the extent of anaerobiosis. Whole-body lactate levels were much greater in the bullheads than the trout, indicating a higher capacity for anaerobiosis, possibly due to a greater fuel supply. Overall, the trout responded to cyanide in a manner similar to that produced by environmental hypoxia whereas the bullheads experienced a gustatory stimulus which masked the hypoxia-like response.     

An overview of stress physiology of fish transport: changes in water quality as a function of transport duration

This study brings an integrated analysis about the relationship between water deterioration and its physiological consequences in live fish transport. The analysis was focused on the transport water and its deterioration, and physiological challenges imposed on the fish. Usual commercial handling procedures employed to mitigate fish stress during transport were discussed. Future topics of research for the establishment of safer fish transport protocols were proposed. Transport was classified into short (≤8 h) or long transport (>8 h). The main issue in short transports should be the prevention of water pH reduction, while in long transports it is the increase in ammonia. Plasma cortisol is the most employed marker for stress and is acutely elevated upon short episodes of transport, but remains elevated even in long‐transport events. Plasma glucose is perhaps a better marker for handling stress. Plasma lactate, pH, osmolality CO₂ and ions should be more often evaluated. Plasma Na⁺ and Cl^‐ are very useful markers of acidosis, due to their respective exchange for H⁺ and , for acid–base regulation. The establishment of species‐specific transport protocols should be preceded by such combined analyses of water and physiological parameters.     

Are hatchery‐reared abalone naïve of predators? Comparing the behaviours of wild and hatchery‐reared northern abalone,Haliotis kamtschatkana (Jonas, 1845)

Abalone populations have declined worldwide, generating interest in enhancement using hatchery‐reared individuals. In many cases, such restoration efforts have met with limited success due to high predator‐induced mortality rates. Furthermore, the mortality rates of outplanted hatchery abalone are often considerably higher than for wild individuals. This study uses northern abalone (Haliotis kamtschatkana) as a case study to determine whether hatchery‐reared abalone behave differently than their wild counterparts. In the field, outplanted hatchery‐reared abalone were significantly less responsive than wild abalone, in terms of number of abalone responding and intensity of response, to nearby movement and to physical contact with an inert probe. Also, when encountering a cue to which all abalone responded (a seastar predator), hatchery‐reared individuals remained subdued. Anti‐predator behavioural deficits in hatchery‐reared abalone were more pronounced in 4‐year‐old individuals than in 1‐year‐old individuals, suggesting an influence of either age or amount of time spent in the hatchery environment. These behavioural differences are expected to increase the vulnerability of hatchery‐reared abalone to predators, and are likely a major cause of their elevated predator‐induced mortality when outplanted.     

Effects of cadmium on the kinetics of calcium uptake in developing tilapia larvae, Oreochromis mossambicus

The toxic effects of Cd²⁺ on Ca²⁺ influx kinetics in developing tilapia (Oreochromis mossambicus) larvae were evaluated. Addition of 20 µg l^-1 of Cd²⁺ to the environment of 0 and 3 day-old larvae competitively inhibited the Ca²⁺ uptake within 4h resulting in a great increase in K_m values for Ca²⁺ influx (19.3 and 17.4 fold, respectively) as compared with their respective controls. Consequently, the actual Ca²⁺ influx of larvae in solutions of 0.2 mM Ca²⁺ are suppressed by 32–45%. Also, 3 day-old larvae were more sensitive to internally accumulated Cd²⁺ than 0 day-old larvae. Although the Ca²⁺ influx in 0 and 3 day-old larvae may be restored to the levels of their respective controls with 24h of being transferred to a 20 µg l^-1 Cd²⁺ solution, total body Ca²⁺ content was significantly reduced in 3 day-old larvae. Increased Ca²⁺ uptake efficiency ensures sufficient Ca²⁺ for normal growth. However, rapid increase in Ca²⁺ influx after hatching also leads to higher Cd²⁺ uptake. Exposure to Cd²⁺ will lead to a drop in body Ca²⁺ content resulting in retardation of larval growth. Therefore, we conclude that if Ca²⁺ uptake is interfered with at this critical stage of development, larvae will not be able to maintain normal levels of body Ca²⁺ and will show signs of Cd²⁺ poisoning.     

Migratory dynamics of stream-spawning longnose gar (Lepisosteus osseus)

Abstract– Literature evidence suggests that lake-dwelling longnose gar (Lepisosteus osseus) enter tributary streams to spawn, Until the present study, the dynamics of this breeding migration had never been investigated quantitatively. During the summers of 1991 and 1992, longnose gar were captured as they entered Weaubleau Creek, Missouri, a tributary of Harry S. Truman Reservoir. The in-stream spawning migration began in early April and ended in late May, and was positively correlated with stream flow and water level, and negatively correlated with water temperature. In-stream residence times ranged from 15 to 94 days, with males exhibiting longer residence times than females. Once in-stream, longnose gar travelled as far as 10 km upstream and occupied certain pools at greater relative frequencies. Although the reason for this preferential utilization is not completely understood, it may relate to pool depth and riffle proximity. Longnose gar disperse from the spawning stream great distances, with gar captured in Weaubleau Creek being recaptured up to 48 km away. This information should provide fisheries biologists the means to consider the reproductive ecology of this species in their conservation and management decisions.     

Nutritional regulation of hepatocyte fatty acid desaturation and polyunsaturated fatty acid composition in zebrafish (Danio rerio) and tilapia (Oreochromis niloticus)

The desaturation and elongation of [1-¹⁴C]18:3n-3 was investigated in hepatocytes of the tropical warm freshwater species, zebrafish (Danio rerio) and Nile tilapia (Oreochromis niloticus). The hepatocyte fatty acid desaturation/elongation pathway was assayed before and after the fish were fed two experimental diets, a control diet containing fish oil (FO) and a diet containing vegetable oil (VO; a blend of olive, linseed and high oleic acid sunflower oils) for 10 weeks. The VO diet was formulated to provide 1% each of 18:2n-6 and 18:3n-3, and so satisfy the possible EFA requirements of zebrafish and tilapia. At the end of the dietary trial, the lipid and fatty acid composition was determined in whole zebrafish, and liver, white muscle and brain of tilapia. Both zebrafish and tilapia expressed a hepatocyte fatty acid desaturation/elongation pattern consistent with them being freshwater and planktonivorous fish. The data also showed that hepatic fatty acid desaturation/elongation was nutritionally regulated with the activities being higher in fish fed the VO diet compared to fish fed the FO diet. In zebrafish, the main effect of the VO diet was increased fatty acid Δ6 desaturase activity resulting in the production of significantly more 18:4n-3 compared to fish fed the FO diet. In tilapia, all activities in the pathway were greater in fish fed the VO diet resulting in increased amounts of all fatty acids in the pathway, but primarily eicosapentaenoic acid (EPA; 20:5n-3) and docosahexaenoic acid (DHA; 22:6n-3). However, the fatty acid compositional data indicated that despite increased activity, desaturation of 18:3n-3 was insufficient to maintain tissue proportions of EPA and DHA in fish fed the VO diet at the same level as in fish fed the FO diet. Practically, these results indicate that manipulation of tilapia diets in commercial culture in response to the declining global fish oil market would have important consequences for fish fatty acid composition and the health of consumers. Scientifically, zebrafish and tilapia, both the subject of active genome mapping projects, could be useful models for studies of lipid and fatty acid metabolism at a molecular biological and genetic level. This revised version was published online in August 2006 with corrections to the Cover Date.     

Production of diatom–bacteria biofilm isolated from Seriola lalandi cultures for aquaculture application

Controlled incorporation of selected microalgae and bacteria in aquaculture systems can be beneficial because they can act as microbiological control. That is why the characteristics of biofilm generated naturally in Seriola lalandi culture cages were analysed, their potential benefit to the growth of larvae was studied, and their controlled use for improving the larval viability and as a vector to improve incorporation of previously studied probiotic bacteria was tested. According to biodiversity results, these biofilms are composed of a diatom–bacteria mix showing a decrease in biodiversity in laboratory culture conditions being dominated by Navicula phyllepta and bacteria of the family Rhodobacteraceae. This can be produced on mesh substrates incorporated in bioreactors with rapid growth rate and adhesiveness. Preliminary results from the addition of substrates with this specific biofilm in larvae culture systems showed that it is consumed by the larvae without negative effects, while positive effects on the viability of larvae in combination with probiotics were observed. Considering preliminary results, the addition of these specific substrates with diatom–bacteria biofilms could be a good improvement for aquaculture systems and together with the use of probiotics can contribute to maintaining a stable and controlled system improving the viability of the larval fish culture in its early stages.