日本海马仔鱼消化系统的组织学研究

日本海马仔鱼消化系统的组织学研究ＡＳＴＵＤＹＯＮＴＨＥＨＩＳＴＯＬＯＧＹＯＦＤＩＧＥＳＴＩＶＥＳＹＳＴＥＭＯＦＬＡＲＶＡＬＳＥＡ－ＨＯＲＳＥ张峰（大连水产学院，１１６０２３）ＺｈａｎｇＦｅｎｇ（ＤａｌｉａｎＦｉｓｈｅｒｉｅｓＣｏｌｅｇｅ，１１６０２３...     

CHANGES IN LIPIDS OF MUSSEL DURING STORAGE

ＣＨＡＮＧＥＳＩＮＬＩＰＩＤＳＯＦＭＵＳＳＥＬＤＵＲＩＮＧＳＴＯＲＡＧＥＸｕｅＣｈａｎｇｈｕ，ＷａｎｇＹｉｓｈｅｎｇ，ＬｉＺｈａｏｊｉｅ，ＬｏｕＷｅｉｆｅｎｇ（ＯｃａａｎＵｎｉｖｅｒｓｉｔｙｏｆＱｉｎｇｄａｏ２６６００３）ＣＨＡＮＧＥＳＩＮＬＩＰＩＤ...     

中国渔船的节能技术

中国渔船的节能技术章可畏（上海水产大学，２０００９０）关键词渔船，节能技术ＥＮＥＲＧＹＳＡＶＩＮＧＴＥＣＨＮＯＬＯＧＹＦＯＲＣＨＩＮＥＳＥＦＩＳＨＩＮＧＢＯＡＴ￥ＺｈａｎｇＫｅｗｅｉ（ＳｈａＮＧＨＡＩＦｉｇｈｅｒｉｅｓＵｎｉｖｅｒｓｉｔｙ，２０００９...     

青蛤生境及生长

青蛤生境及生长ＨＡＢＩＴＡＴＡＮＤＧＲＯＷＴＨＯＦＣＬＡＭ（ＣＹＣＬＩＮＡＳＩＮＥＮＳＩＳＧＭＥＬＩＮ）￥ＹｕＹｅｓｈａｏａｎｄＷａｎｇＨｕｉ（ＥａｓｔＣｈｉｎａＳｅａＦｉｓｈｅｒｉｅｓＲｅｓｅａｒｃｈＩｎｓｔｉｔｕｔｅ，ＡＳＦ，Ｓｈａｎｇｈａｉ２０...     

鲇鱼淋巴样器官的发育

鲇鱼淋巴样器官的发育ＯＮＴＯＧＥＮＹＯＦＬＹＭＰＨＯＭＹＥＬＯＩＤＯＲＧＡＮＳＩＮＣＡＴＦＩＳＨ（ＳＩＬＵＲＵＳＡＳＯＴＵＳＬ．）￥ＺｈｏｎｇＭｉｎｇｃｈａｏ；ＨｕａｎｇＺｈｅ（ＤｅｐａｒｔｍｅｎｔｏｆＢｉｏｌｏｇｙ，ＺｈｏｎｇｓｈａｎＵｎｉｖｅｒｓ...     

中国对虾血淋巴液中的凝集素

用饱和硫酸铵盐析、Ｓ－２００－ＨＲ凝胶层析、ＤＥＡＥ－ｓｅｐｈａｒｏｓｅ离子交换层析从中国对虾（ｆＰｅｎａｅｕｓ ｃｈｉｎｅｎｓｉｓ）血淋巴液中分离出１种凝集素，通过ＳＤＳ－ＰＡＧＥ测定，该凝集素由２个亚基组成，分子量分别为８００００、７５０００；此凝集素能凝集牛、马、鼠、兔、鸡、羊和人Ａ、Ｂ、Ｏ型红细胞，其凝集活性不被Ｄ－甘露糖、Ｄ－果糖、Ｄ－乳糖、乙酰半乳糖、神经氨酸、Ｄ－葡萄糖、Ｄ－半乳糖和     

褐藻多糖类化合物对卵磷脂氧化的抑制作用

褐藻多糖类化合物在乳化体系中对卵磷脂（ＰＣ）氧化具抑制作用，通过测定卵磷脂氢过氧化物（ＰＣ－ＯＯＨ）含量，确定各化合物的抗氧化能力。结果表明：在自由基引发剂（ＡＡＰＨ）作用下，褐藻多糖类化合物（褐藻胶ＡＬＧ、磺化褐藻胶ＳＡＬＧ、甘糖酯ＰＧＭＳ、藻酸双酯钠ＰＳＳ，５０μｍｏｌ／Ｌ）对ＰＣ氧化的抑制作用均比ＶＣ（６０μｍｏｌ／Ｌ）好，但不如ＶＥ（３０μｍｏｌ／Ｌ）的效果好；抑制顺序是：ＶＥ＞ＳＡＬＧ＞ＰＧＭＳ＞ＰＳＳ＞ＡＬＧ＞ＶＣ。     

COMPARISON OF LIPIDS AND NITROGENOUS EXTRACTIVES OF DANUBLAN WELS AND CHINESE CATFISH

ＣＯＭＰＡＲＩＳＯＮＯＦＬＩＰＩＤＳＡＮＤＮＩＴＲＯＧＥＮＯＵＳＥＸＴＲＡＣＴＩＶＥＳＯＦＤＡＮＵＢＬＡＮＷＥＬＳＡＮＤＣＨＩＮＥＳＥＣＡＴＦＩＳＨＸｕｅＣｈａｎｇｈｕｏ（ＯｃｅａｎＵｎｉｖｅｒｓｉｔｙｏｆＱｉｎｇｄａｏ，２６６００３）ＷａｎｇＪｉａ...     

实现我国水产系统文献信息资源共享的探讨

实现我国水产系统文献信息资源共享的探讨ＡＤＩＳＣＵＳＳＩＯＮＯＮＴＨＥＬＩＢＲＡＲＹＲＥＳＯＵＲＣＥＳＨＡＲＩＮＧＩＮＣＨＩＮＡＦＩＳＨＥＲＹＣＩＲＣＬＥＳ杨宁生（中国水产科学研究院渔业综合信息研究中心，北京１０００３９）ＹａｎｇＮｉｎｇｓｈｅｎｇ（...     

海蜇染色体组型分析

海蜇染色体组型分析郭平（辽宁省海洋水产研究所，大连１１６０２３）关键词根口水母，海蜇，染色体组型ＴＨＥＫＡＲＹＯＴＹＰＥＯＦＲＨＯＰＬＣＭＡＥＳＣＵＬＥＮＴＡ￥ＧｕｏＰｉｎｇ（ＭａｒｉｎｅＦｉｓｈｅｒｉｅｓＲｅｓｅａｒｃｈＩｎｓｔｉｔｕｔｅｏｆＬｉａ...     

锯缘青蟹血清凝集素的特性和盐析提取研究

对锯缘青蟹(Scylla serrate)血清凝集素的凝集特性进行了研究。结果显示:锯缘青蟹血清凝集素对人、兔、鼠、鸽子、中华鳖、鲫红细胞均有凝集作用,其中对小鼠红细胞凝集活性最强,血凝活性可达29;凝集素活性最适pH 6.0~7.4;盐度对凝集活力有较大影响,NaC l浓度在0.4~0.5 mol/L时基本失活;未纯化血清凝集素对Ca2+、Mg2+未表现出依赖性;凝集素活性能被N-乙酰葡萄糖胺特异性抑制。对青蟹血清凝集素进行硫酸铵分级沉淀分离后,发现凝集素活性主要分布在25%饱和度硫酸铵沉淀区,该组分凝集比活比血清高27.44倍,SDS-PAGE结果表明主要蛋白带型为79 ku、75 ku、72 ku。     

三疣梭子蟹C-型凝集素的原核表达和活性检测

通过RT-PCR扩增三疣梭子蟹C-型凝集素（C-type lectin like-domain protein,CTLD）基因序列,将目的基因克隆入质粒pET32a,构建原核表达重组质粒pET32a-CTLD.将重组质粒转化入大肠杆菌BL21中,通过IPTG诱导CTLD的表达,对其表达产物进行SDS-PAGE和Western blot 检测.用His GraviTrap Ni亲和层析柱及超滤离心管纯化目的蛋白,并对其进行活性检验.结果表明,构建得到CTLD基因的原核表达载体,双酶切鉴定得到与预期片段相符的外源基因插入片段,经测序与目的序列完全一致;诱导表达出相对分子质量（M） 39 600的目的蛋白,经SDS-PAGE和Western blot检测,融合蛋白成功表达;重组C-型凝集素对兔的红细胞没有明显的凝集作用,但对小鼠红细胞的凝集效价是22,而对所选5种细菌的凝集作用存在差异,其中对大肠杆菌具有明显的凝集作用,而对溶藻弧菌、哈维氏弧菌、鳗弧菌和金黄色葡萄球菌均无凝集现象.本研究结果为进一步研究C-型凝集素在免疫防御中的作用与地位提供依据.     

A novel mannose-binding lectin from plasma of Labeo rohita

A new mannose-binding lectin was purified from plasma of freshwater fish, rohu (Labeo rohita) by ammonium sulphate precipitation followed by DEAE-cellulose ion-exchange chromatography. The hemagglutinating activity is strong for neuraminidase-treated rabbit erythrocytes. Mannose, glucose and their derivatives inhibit hemagglutination. The apparent molecular weight was determined to be 210 kDa in native PAGE. Analysed on SDS-PAGE under reducing and non-reducing conditions, the protein migrated as a single band of mol.wt. 40,000. The lectin is acidic in nature showing a pI of 4.5. It is a glycoprotein containing complex glycan part as indicated by carbohydrate analysis using high-pressure anion exchange chromatography with a pulsed amperometric detector. The N-terminal sequence (WLNGIGTQIPMKITT) shows no significant homology with known proteins. It appears to be a C-type lectin as Ca²⁺ is essential for carbohydrate-binding activity. Methyl -α- D-mannopyranoside was found to be the most potent inhibitor among the monosaccharides and disaccharides tested. Purified lectin was found to induce intracellular superoxide production following opsonization of E. coli by its own macrophages. This revised version was published online in August 2006 with corrections to the Cover Date.     

Purification and characterization of a lectin from the starfish Asterias amurensis

In this study, we describe a novel lectin (designated AMUL) from the Northern Pacific starfish Asterias amurensis. AMUL was purified from the coelomic fluid by affinity chromatography followed by size-exclusion chromatography. The native molecular mass of the lectin was found to be approximately 270 kDa by size-exclusion chromatography using a Superdex 200 column. AMUL showed distinct bands at approximately 14 or 18 kDa on SDS-PAGE under nonreducing or reducing conditions, respectively. The hemagglutination activity of AMUL against rabbit erythrocytes was strongly inhibited by the two sialic acids N-acetylneuraminic acid and N-glycolylneuraminic acid. The glycoproteins tested showed no inhibition of hemagglutination. The first 28 amino acid residues of AMUL were determined by automated Edman degradation, showing that AMUL has considerable sequence homology with C-type lectins from other echinoderms. These results show that AMUL is a novel echinoderm-derived sialic-acid-specific lectin that belongs to the C-type lectin superfamily.     

中国对虾血淋巴凝集素的血凝活性与促噬活性

1999年8月，运用血凝活性测定法分别检测中国对虾血淋巴弹簧集素，血淋巴液、血细胞、肌肉、肠、肝胰腺和甲壳的血凝活力，并运用吖啶橙血细胞吞噬测定法检测血细胞的吞噬活力，同时测定了经注射中国对虾副粘病毒提纯液后血淋巴凝集素血凝活力及血淋巴液抗菌活力、酚氧化酶活力、溶菌活力，结果表明，血淋巴凝集素主要分布在血淋巴液和血细胞中；该凝集素对人（A、B、O型），鸡和兔红细胞及其醛化红细胞的凝集具有一定专一性；对血细胞吞噬异物具有促进功能；注射副粘病毒提纯液后，血淋巴凝集素凝集活力变化和血淋巴液抗菌活力，酚氧化酶活力、溶菌活力变化相一致。     

贻贝凝集素生物活性的研究

从海洋无脊椎动物贻贝中分离出的凝集素(CGL),为N-乙酰半乳糖胺/半乳糖(GalNAc/Gal)特异性的凝集素。经SDS-聚丙烯酰胺凝胶电泳测定,其相对分子量为18 000。其血凝活性不依赖钙离子的存在。在植物凝集素(PHA)存在的条件下,CGL具有体外促进小鼠脾细胞增殖的活性。同时,它还具有抑制酶活性,其抑制50?E的浓度(IC50)为6.82 mg/m l和促进体外离体兔回肠蠕动的作用。     

Structural characterization and antitumor and mitogenic activity of a lectin from the gill of bighead carp (Aristichthys nobilis)

In this study, we investigated the gross structure, secondary structure, and antitumor and mitogenic activity of GANL, a lectin from the gill of bighead carp (Aristichthys nobilis). We used infrared spectroscopy, β-elimination, and circular dichroism spectroscopy to determine the structure of GANL. We measured antiproliferation activity against six human tumor cell lines and mitogenic activity against murine splenocytes using the MTT assay. Based on infrared spectroscopy and β-elimination, we conclude that GANL is a glycoprotein. The protein and carbohydrate moieties are joined by O-glycosidic linkage. A circular dichroism spectroscopic analysis revealed that the secondary structure of GANL consists of α-helices (34.8 %), β-sheets (12.1 %), β-turns (24.5 %), and unordered structures (33.0 %). GANL exerted potent antitumor activity against the HeLa cell line (IC₅₀ = 11.86 μg/mL) and a mitogenic effect on murine splenocytes in the MTT assay. GANL, a lectin that is isolated from the gills of bighead carp, is a glycoprotein with potent antitumor and mitogenic activity.     

Induction of phenoloxidase and other immunological activities in the humoral fluids of amphioxus Branchiostoma belcheri challenged with Lipopolysaccharide (LPS)

The knowledge concerning the humoral immunity is scarce in amphioxus Branchiostoma belcheri. This study measured the humoral parameters including phenoloxidase (PO) activity, lysozyme activity, antimicrobial activity, microbial agglutinin, and hemagglutinin in amphioxus humoral fluids before and after lipopolysaccharide (LPS) challenge. Humoral fluids from unchallenged Branchiostoma belcheri (B. belcheri) had PO activity, lysozyme, antimicrobial, microbial agglutinating, and hemagglutinating activities, which may represent part of the baseline level of innate immunity in this organism. After challenge with LPS, many humoral parameters were all increased significantly including the PO activity, lysozyme activity, growth-inhibiting activities against Gram-negative bacteria Escherichia coli (E. coli) and Vibrio alginolyticus (V. alginolyticus), growth-inhibiting activities against Gram-positive bacteria Staphylococcus aureus (S. aureus) and Bacillus subtilis (B. subtilis), microbial agglutinating activities against Micrococcus lysodeikticus (M. lysodeikticus), B. subtilis, and S. aureus, and hemagglutinating activities against rabbit and human A and O erythrocytes. In contrast, the agglutinating activities against V. harveyi and E. coli and the hemagglutinating activity against human B erythrocytes in the humoral fluids were reduced in response to LPS challenge. It appears that the humoral fluids of B. belcheri contain components that are able to differentiate different microbes and different human blood cell types.     

Detection of the first lectin with antimicrobial activity present in serum of the Amazonian fish tambaqui Colossoma macropomum

Tambaqui Colossoma macropomum is the most important Amazonian native species in South American aquaculture. Innate immunity at least partially depends on the recognition of pathogen-associated molecular patterns by receptor-recognizing pathogens (PRRs). Some PRRs have been characterized in fishes, and several studies have focused on the role of lectins in the immune system of various fishes. Lectins are proteins that specifically recognize carbohydrates and which have important biological functions. Tambaqui serum lectin (ComaSeL), which was identified on the basis of its hemagglutinating activity, was pre-purified, biochemically characterized, and used in assays of antibacterial activity against pathogenic bacteria in freshwater fishes. A study of the seasonality of this lectin was performed. Comasel activity was stable at a pH between 4.0 and 9.0 and lost 100?% of its activity at 70?°C. It recognized the carbohydrates d-galactose, 1-O-methyl-d-galactopyranoside, and d-fucose, showing antibacterial activity for Gram-negative bacteria. Its activity showed significant differences between the summer and winter (p?<?0.05, Tukey test), thereby corroborating observations that tambaqui becomes more susceptible to mortality from diseases caused by bacteria and fungi during the winter. With this information, new tools may be developed for gaining a better understanding of the role of these proteins in the immune system of the tambaqui, ultimately resulting in the improved management of this fish by pisciculturists.     

军山湖中华绒螯蟹血清凝集素特性分析

从军山湖中华绒螯蟹(Eriocheir sinensis)获得血清,用人A、B、AB、O型红细胞、鸡红细胞及部分细菌菌体检测了血清的凝集活力,同时进行了糖抑制、热稳定性及pH影响试验。结果显示血清能凝集人四种血型红细胞和鸡红细胞,其中对人A型红细胞凝集活力最高;能够和大肠杆菌发生凝集反应,但不能和枯草芽孢杆菌、金黄色葡萄球菌发生凝集反应;凝集条件为pH5.0~5.8、温度4~37℃;1.0%的D-葡萄糖、D-果糖、蔗糖、乳糖、麦芽糖均能不同程度地抑制血清与人A型红细胞的凝集作用。