Evaluation of Seven Algal Diets for Spat of the Pacific Scallop Argopecten ventricosus

The nutritive value of Isochrysis galbana (Tahitian), Chaetoceros calcitrans, Chaetoceros muel-leri . and their bi- and trialgal combinations for Pacific scallop Argopecten ventricosus spat culture was studied. Ingestion rate, growth, survival, and biochemical composition were the variables evaluated. Growth results showed C. calcitrans and I. galbana + C. calcitrans diets have the best nutritive values. Poorest results were obtained with I. galbana diet. The acceptability and a high lipid content of C. calcitrans could explain the good results of C. calcitrans and I. galbana + C. calcitrans diets along with a better balanced diet effect in the bialgal mixture. The low growth of the scallops using the C. calcitrans + C. muelleri diet, despite the high ingestion rate, is explained by a low energy content and an unbalanced nutrient supply. The gross biochemical composition of the diets did not show a clear relationship to spat gross biochemical composition.     

Effect of Ozone Treatment on Cultures of Nannochloropsis oculata, Isochrysis galbana, and Chaetoceros gracilis

This research evaluated ozone treatment of three unicellular algal species commonly used in the culture of marine larvae. Isochrvsis galbana and Nannochloropsis oculata were found to be resistant to total residual oxidants (TRO); whereas, Chaetoceros gracilis was relatively sensitive to TRO. Isochrvsis galbana remained in log-phase growth after treatment with 0.05 mg/L to 0.50 mg/L TRO, and N. oculata exhibited strong growth after being exposed to TRO levels up to 0.67 mg/L. Only TRO levels of 0.92 mg/L and 0.90 tng/L reduced cell counts of N. oculata and I. galbana , respectively. Furthermore, algal cultures having higher concentrations at the time of ozone treatment may respond more favorably to the treatment. Initially, C. gracilis was reduced in cell count at TRO levels above 0.06 mg/L, but log-phase growth was resumed 2 d after exposure to TRO levels as high as 0.31 mg/L. It is concluded that I. galbana and N. oculata can be successfully treated with ozone in the mass production stages without hampering the timely production of concentrated cultures. Although C. gracilis was found to be relatively sensitive, ozone treatment of starter cultures can be performed routinely with this species. We have demonstrated that ozone treatment of algal cultures is a feasible technique, but studies need to be conducted on ozone treatment of other species of unicellular algae and on ozone treatment of varying concentrations of algae in order to enhance the applications of this technique. Also, in order to determine the extent of disinfection of algal cultures, bacterial and viral composition of the cultures should be evaluated in future studies.     

Population Growth and Production of Apocyclops dengizicus (Copepoda: Cyclopoida) Fed on Different Diets

This study was carried out to investigate the effects of various diets: 4 monoalgal diets: Nannochloropsis oculata (N), Isochrysis galbana (I), Chaetoceros calcitrans (C), and Tetraselmis tetrathele (T); 4 mixed algal diets: N+I+C+T(NICT), N+I+C(NIC), C+T(CT), and I+T(IT); and 2 nonalgal diets: baker's yeast (BY) and prepared shrimp feed (SF) on population growth and density of Apocyclops dengizicus . The type and density of diet had significant effects on the growth and density of A. dengizicus ( P  < 0.01). Of tested diets, T and CT were optimum diets due to higher density and growth rate of A. dengizicus compared to other diets. Their high dietary value was related to the higher contents of polyunsaturated fatty acid, particularly docosahexaenoic acid (22:6n-3), eicosapentaenoic acid (20:5n-3), and arachidonic acid (20:4n-6) compared to A. dengizicus cultured on other diets. The results of the present study illustrated that T. tetrathele was the most suitable food for the culture of A. dengizicus .     

Effects of starvation and feeding on the fatty acid profiles of Stage I phyllosoma of the spiny lobster, Jasus edwardsii

Fatty acid analyses were conducted on newly hatched and 8‐day‐old‐starved and fed Stage I phyllosoma larvae of the spiny lobster, Jasus edwardsii. Fed animals were offered excess 1.5 mm juvenile Artemia (enriched using the alga Isochrysis galbana, Tahitian isolate, T. iso.). After 8 days, there were significant increases in larval dry weight and the proportion of lipid in fed phyllosoma, whereas these parameters decreased in starved phyllosoma. The abundance of the saturated fatty acids 16 : 0 and 18 : 0 increased in both starved and fed phyllosoma, whereas the main monounsaturated fatty acids 16 : 1n‐7, 18 : 1n‐9 and 18 : 1n‐7 increased with feeding but decreased with starvation. There were no significant differences in the relative proportions of the highly unsaturated fatty acids (HUFAs) arachidonic (AA, 20 : 4n‐6), eicosapentaenoic (EPA, 20 : 5n‐3) and docosahexanoic (DHA, 22 : 6n‐3) acids between newly hatched and starved animals, whereas quantitatively DHA decreased with starvation and feeding. The DHA/EPA ratio was significantly lower in the starved and fed phyllosoma (0.5) compared with that found in the newly hatched phyllosoma (0.9). The lipid profiles of the newly hatched, starved and fed phyllosoma contained large amounts of n‐6 fatty acids resulting in low n‐3 : n‐6 ratios (2.8, 2.7 and 1.6 respectively). The importance of these results and the ability of enriched Artemia to provide a suitable fatty acid profile for this species are discussed.     

Evaluation of Five Microalgae Diets for Juvenile Pen Shells Atrina maura

–Juvenile pen shells Atrina maura were fed for 21 d with equal daily rations, equivalent to 6.5% of their live weight, of monoalgal cultures of Tetraselmis suecica, Isochrysis galbana, Chaetoceros muelleri, Chaetoceros calcitrans , and Thalassiosira pseudonana. Pen shell survival was 100% in all cases. All microalgae diets produced shell growth, and live and ash-free dry weight increases. However, the two Chaetoceros strains gave significantly better results, with shell height increases of 4.7 to 2.9 mm, live weight gains of 0.5 to 0.3 g, and final ash-free dry weights of 0.16 to 0.14 g after 3 wk. These results are not related to the algae gross biochemical composition, suggesting that other factors are involved in their nutritional value for this mollusc species.     

大鳍鳠产卵前后肌肉脂肪酸组成变化的研究

采用毛细管气相色谱法,对产卵前后大鳍鳠肌肉中的脂肪酸组成进行了测定。结果表明,大鳍鳠肌肉中脂肪酸种类丰富,含有22种脂肪酸,油酸(C18:1n-9)所占比例最高,其次为软脂酸(C16:0)、棕榈酸(C16:1n-7)、亚油酸(C18:2n-6),所占比例分别为22.38%~37.57%、18.86%~23.95%、7.00%~11.52%、7.46%~9.97%。EPA和DHA在大鳍鳠肌肉中占有较高的比例,EPA占总脂肪酸的5.39%,DHA占5.44%。在野生大鳍鳠产卵前后的22种脂肪酸中有8种饱和脂肪酸(SFA),6种单不饱和脂肪酸(MUFA)和8种多饱和脂肪酸(PUFA),大鳍鳠肌肉中脂肪酸在产卵前后表现出差异。     

Evaluation of Fatty Acid Enrichment of Live Food for Yellowtail Snapper Ocyurus chrysurus Larvae

Abstract— The purpose of this study was to examine the effect of varying dietary levels of highly unsaturated fatty acids (HUFA) in Live prey on the standard length, specific growth rate, survival, and fatty acid composition of yellowtail snapper Ocyurus chrysurus larvae. Two experiments were conducted utilizing rotifers and Artemiu enriched with live algae ( Isochrysis galbana or Nannochloris oculata ) or commercial preparations (Aquagrow Advantage, Aquagrow Advantage plus Aquagrow arachidonic acid, and Algarnac 2000). Larval growth and fatty acid composition were evaluated during the rotifer, B rachionus plicatilis , and Artemia feeding periods and survival rates were calculated at the termination of each trial (18 or 20 d after hatching). In general, prey enriched with the commercial products contained higher levels of docosahexaenoic acid, eicosapentaenoic acid, n-3 HUFA, and × HUFA than those enriched with live algae. The addition of arachidonic acid to the Aquagrow Advantage enrichment medium significantly increased the amount of this fatty acid in rotifers but not in Artemia . At the end of the growth trials, larval standard length was highest when larvae were fed prey enriched with I. galbanu (6.4 mm) or commercial preparations (6.7–7.1 mm) versus N. oculatu (5.2 mm). Furthermore, larvae fed prey enriched with commercial preparations had significantly ( P < 0.05) higher survival rates (2.2-5.9%) than those fed prey enriched with live algae (1.1-1.4%). These results suggest that yellowtail snapper larvae require dietary levels of HUFA beyond those achieved by enriching prey with live N. oculata or I. galbana     

The growth rate of two species of microalgae used in shellfish hatcheries cultured under two light regimes

Abstract. The production potential of any shellfish hatchery depends on the capacity of its algal system. The hypothesis that microalgal cultures grown under 12:12h light:dark photoperiod may produce the same cell densities as those using constant light is tested.
Two species of marine microalgae used in shellfish hatcheries. Chaetoceros gracilis Schütt and Isochrysis galbana clone T-iso, were grown in cultures at 18°C, under continuous light (1.43 × 10⁷μ^-2day^-1) and 12:12h light:dark cycles (2.87 × 10⁷μE m^-2 day^-1). Both light regimes provided equal amounts of light per day. In the continuous light cultures the mean doublings per day for exponentially growing cells were 1.37 and 1.49 for C. gracilis and I. galbana respectively and for the 12:12h light:dark cycles were 1.47 and 1.56 respectively. After 14 days of growth, the numbers of cells per unit of volume showed no significant differences between the two light regimes. The results are discussed in terms of a review of other authors' findings and in terms of the usefulness of the continuous light method in producing algae to be used in shellfish hatcheries.     

Changes in oil content,lipid class and fatty acid composition of the microalga Chaetoceros calcitrans over different phases of batch culture

The diatom Chaetoceros calcitrans is a microalgal species used as food for larva in aquaculture for many species worldwide. Chaetoceros calcitrans is an important source of omega 3 long chain (C ≥ 20) polyunsaturated fatty acids (n‐3 LC PUFA), chiefly eicosapentaenoic acid (EPA, 20:5n‐3). This article reports lipid content, lipid class composition and fatty acid profiles of each lipid class during the growth cycle of batch cultures of C. calcitrans. Total lipid content and the concentration of neutral lipid were highest in the late stationary growth phase (day 12). However, the amount of EPA was highest during the logarithmic growth phase (1.24 pg/cell on day 4). EPA was initially concentrated in the glycolipid fraction, but this fraction decreased during logarithmic growth, coinciding with the increase in neutral lipid. Docosahexaenoic acid (22:6n‐3, DHA) (0.91 pg/cell) was reported as a major fatty acid (>10 mg/100 g) in all lipid classes on day 1. DHA was depleted quickly from the neutral lipid and glycolipid classes, but at a slower rate from the polar lipid fraction. This work confirms that C. calcitrans is a good source of lipid, in particular EPA, for larval and adult filter feeders in aquaculture.     

不同饵料对毛蚶幼体发育的影响

在毛蚶人工育苗过程中,使用球等鞭金藻、角毛藻、小球藻、扁藻4种藻类的不同组合投喂毛蚶幼体。结果表明,饵料种类越多,变态率越高,生长速度越快;球等鞭金藻、角毛藻、小球藻、扁藻搭配(体积比1:1:1:1)变态率为30%,出库需(壳长1 mm)27 d;球等鞭金藻、角毛藻、小球藻搭配(体积比1:1:1)变态率为29%,出库需29 d;球等鞭金藻、小球藻搭配(体积比1:1)变态率为24%,出库需31 d;角毛藻、小球藻搭配(体积比1:1)变态率为19%,出库需33 d;单一投喂小球藻,变态率为3%,出库需44 d。说明不同饵料间的营养可以互补,多种饵料搭配能为幼体发育提供更全面的营养,饵料多样性是影响幼体培养效果的重要因素。     

Fatty Acid Porfiles of Farm-Raised Catfish Fillet:

Fatty acid profiles of the total lipids and the phospholipid classes of farm-raised channel catfish (Ictalurus punctatus) fillets were analyzed. Monoenes represented 49.22 mol% of the total lipid fatty acids while polyunstaturated fatty acids (PUFA) accounted for 19.37 mol%. The total n-3 PUFA content was low (4.22 mol%). The total saturated fatty acids as 31.44 mol %. Catfish contained phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylinositol (PI), phosphatidylserine (PS), lysophosphatidylcholine (LPC), and sphingomyelin (SPH). Analysis for the individual phospholipid class fatty acid profiles indicated that PI had a high concentration of 18:0 (33.20 mol%) and 20:4 n-6 (13.08 mol%). Phosphatidylethanolamine had the highest concentrations of combined eicosapentaenoic acid, and docoshahexaenoic acid, of 17.37 mol%. Higher ratios of n-3/n-6 PUFA were found in the phospholipid classes than the total lipids. LPC and SPH did not contain measurable PUFA of the n-3 series. The level of 18:1 n-9 in PC, PE, and LPC was approximately 31 mol% whereas in PI, PS, and SPH, the level was approximately 16 mol%. The understanding of the basic distribution of fatty acids in the individual phospholipid classes of channel catfish may be an essential first step in explaining the protective role of 18:1 n-9 and n-3 PUFA against thrombotic and cardiovascular disorder in humans.     

The Biochemical Composition of Chaetoceros muelleri (Lemmermann Grown) with an Agricultural Fertilizer

The aim of this work was to demonstrate that the source of nitrogen of microalgae growth media has an effect on the biochemical composition by comparing the composition of Chaetoceros muelleri grown in semicontinuous cultures with 30% daily dilutions with an agricultural fertilizer containing three nitrogen sources (AF) and with the traditional f/2 medium. The cell yields, proximate composition, and total essential and nonessential amino acid contents of C. muelleri were similar, but phenylalanine and glutamate were significantly higher with f/2, and alanine was higher with AF. Monounsaturated fatty acids were higher in AF‐grown cultures, but polyunsaturates were higher with f/2. Among polyunsaturates, 20:5n‐3 (eicosapentaenoic acid, EPA) was higher with AF (17.16%) than with f/2 (13.44%), whereas f/2 yielded cells richer in 20:4n‐6 (arachidonic acid, ARA: 2.08% vs. 1.38%) and 22:6n‐3 (docosahexaenoic acid, DHA: 6.86% vs. 3.80%). Our results provide evidence that different nitrogen sources may cause variations in the biochemical profile of C. muelleri.     

Nutritional Properties of the Marine Rotifer Brachionus plicatilis Fed the Freshwater Microalgae Selenastrum capricornutum

This study examined the effects of storage time on the fatty acid composition of freshwater Selenastrum capricornutum algal paste under 4 C refrigeration, the fatty acid composition of rotifers fed the fresh and stored algal paste, and the toxicological properties of the algal paste. Microalgae were produced in a hydraulically integrated serial turbidostat algal reactor (HIS-TAR), harvested as a paste, refrigerated and analyzed every 2 wk. Fresh Selenastrum capricornutum paste had almost three times greater concentration of unsaturated fatty acids than saturated fatty acids. Over 50% of the unsaturated fatty acids were made up of n-3 and n-6 fatty acids. Total unsaturated, n-3 group and n-6 group fatty acids decreased ( P 0.05) during storage, including the nutritionally important fatty acids (C20:4n6, C20:5n3, C22: 6n3). Rotifers fed 2– and 4-wk-old algal paste had a significantly lower ( P 0.05) total unsaturated fatty acid percentage and significantly greater ( P 0.05) total saturated fatty acids than those fed fresh algae. There were no significant changes in the n-6 group fatty acid level in the 4-wk-old paste feeding study or for the n-3 or n-6 groups in the 2-wk-old paste feeding study as compared to fresh algae. The levels of the three nutritionally important fatty acids (C20:4n6, C20:5n3, C22:6n3) did not differ between rotifers fed fresh and stored algae. Stored algae did not present toxicity to rotifers and Daphnia at the normal feeding concentration. These results indicated that the use of refrigerated freshwater algal paste for production of rotifers results in live feed with adequate nutritional properties for marine larviculture. This could eliminate the costs associated with production of marine algae, which could be replaced with freshwater algae, and may provide an alternative to cryopreservation.     

A baseline study on lipid and fatty acid composition in the pearl oyster, <Emphasis Type="Italic">Pinctada fucata</Emphasis>

The pearl oyster Pinctada fucata is cultured for pearl production in China, but its germplasm is degenerated. Thus, lipid and fatty acids were investigated in P. fucata based on different tissues, body sizes and condition indexes using GC–MS to provide a lipidomic baseline for evaluation of breeding variety in terms of physiology. The major fatty acids over all samples included saturated (C16:0 and C18:0), monounsaturated [C16:1(n-7), C18:1(n-7) and C18:1(n-9)], and polyunsaturated fatty acids [arachidonic acid: C20:4(n-6), EPA: C20:5(n-3) and DHA: C22:6(n-3)]. Relative percentages of lipid contents of the mantle and visceral mass were, respectively, 40.75 and 42.03 %, significantly higher than adductor muscle (14.48 %) and gill (17.15 %). Fatty acid analysis showed that there were 15 types of fatty acids in adductor muscle, 17 types in mantle and gill, respectively, and 19 types in visceral mass. Meanwhile, total fatty acids, SFA, MUFA and PUFA were the highest in visceral mass (P < 0.05). Yet the lipid contents and fatty acid compositions showed no significant differences between body sizes or condition indexes based on visceral mass. The observations above suggested that visceral mass and mantle are the major tissues for P. fucata to store lipids and thus are important candidate tissues for lipidomic study.     

Dynamics of Total Body Fatty Acids During Early Ontogeny of Pikeperch (<Emphasis Type="Italic">Sander lucioperca</Emphasis>) Larvae

The fatty acid composition of pikeperch (Sander lucioperca) was determined according to their physiological status, during starvation (10 days) and feeding (28 days). In starved larvae, polyunsaturated, monounsaturated and saturated fatty acids were utilized as metabolic substrates until day 9. At day 10, all fatty acid levels remained stable or, at least, increased in larval body. Among fatty acids, docosahexaenoic acid 22:6 n-3 was used preferentially (20.3% from total fatty acids utilized) followed by palmitoleic acid 16:1 n-7 (13.9%) and then by oleic 18:1 n-9 (12.3%), linoleic 18:2 n-6 (10.1%), linolenic 18:3 n-3 (9.7%) and eicosapentaenoic 20:5 n-3 (9.1%) acids. On the other hand, arachidonic acid 20:4 n-6 was utilized very lowly (0.3%). In fed larvae, no utilization of body fatty acids was observed during the experiment. It seems that energy requirements (and others) of fed larvae were satisfied by feed.     

Preliminary Results on the Fatty Acid Composition of Freshwater Crayfish, Astacus astacus and Pacifastacus leniusculus, Held in Captivity

The fatty acid compositions of the tail muscle and hepatopancreas of captive noble crayfish Astacus astactus and of signal crayfish pacfastacus leniusculus were investigated. The major fatty acids in the tail muscle of A. astacus were 16:0. 18:ln-7 + 9, 20:4n–6 and 20:5n–3 and the fatty acid composition of hepatopancreas was principally 16:0, 18:In-7 + 9 and 20: ln-9+ 11. These animals had been fed marine fish (sprat and herring) for 2 mo. They were kept in a flowthrough system with gravity-fed fresh water from a nearby lake with a high concentration of calcium and pH above 8. The major fatty acids in the tail muscle of P. leniusculus were 16:0. 18:ln-7+9, 18:2n–6 and 20:5n–3, and in the hepatopancreas 16:0, 18:1n–7 + 9 and 18:2n–6. These animals had been fed mainly plant material (green peas, maize and alder leaves) in a different laboratory for a period of about 2 yr and kept in a flow-through system with ambient water of pH around 7. In A. astacus the total fatty acid content was nearly 13 times higher in the hepatopancreas than in the tail muscle, however, three fatty acids 18:0, 20:4n–6 and 20:Sn-3 were, respectively, 289, 403, and 320% more concentrated in the fatty acids of tail muscle than in the hepatopancreas. Total fatty acids of the hepatopancreas of male P. Ieniusculus was 42 times higher than in the tail muscle and was three times greater than in the hepatopancreas of A. astacus .     

Determination of amino acid and fatty acid composition of goldband goatfish [Upeneus moluccensis (Bleeker, 1855)] fishing from the Gulf of Antalya (Turkey)

In this study, we aimed to determine the basic food components, fatty acids and amino acids, and variations in these components with months in goldband goatfish (Upeneus moluccensis) that fishing from Gulf of Antalya. As a result of the analyzes, the crude fat values were determined between 1.43 and 3.78%, and the crude protein values were determined between 20.79 and 22.16%. The most abundant fatty acids were determined: palmitic acid (C16:0), stearic acid (C18:0), palmitoleic acid (C16:1c9), oleic acid (C18:1c9), linoleic acid (C18:2n-6), eicosatrienoic acid (C20:3n-3), arachidonic acid (C20:4n-6), eicosapentaenoic acid (C20:5n-3), docosapentaenoic acid (C22:5n-6), and docosahexaenoic acid (C22:6n-3). The most abundant amino acids were determined lysine and leucine, aspartic acid, glutamic acid, alanine, and glycine. The differentiations of essential nutrient components, fatty acids, and amino acids were found generally significant (P < 0.05).

     

The uptake and esterification of radiolabelled fatty acids by enterocytes isolated from rainbow trout (Oncorhynchus mykiss)

To study the intestinal fatty acid absorption in fish in vitro, enterocytes were isolated from the intestine of rainbow trout and incubated with an equimolar mixture of seven fatty acids [16:0, 18:1n-9, 18:2n-6, 18:3n-3, 20:4n-6, 20:5n-3 and 22:6n-3] in which the component carrying a radioactive label was varied. The fatty acid mixture was presented in the form of micelles formed by sonication with sodium taurocholate. Control studies showed that the presence of sodium taurocholate in the incubation medium caused an immediate 23% increase in the mortality of the cells but the remaining cells were viable. The effect of the bile salt on cellular permeability was evident at longer exposure periods. The proportions of 14C-labelled 20:5n-3 and 22:6n-3 present as monomeric fatty acids in the micellar solutions were higher than those of 16:0, 18:1n-9, 18:2n-6, 18:3n-3 and 20:4n-6. The rates of uptake of 14C-labelled 20:4n-6, 20:5n-3 and 22:6n-3 by enterocytes were significantly lower than those of the other fatty acid substrates over the first minute of incubation but no significant differences in uptake rate between fatty acids were obvious over a 15 min incubation period. Notable differences were observed between substrates in their distribution pattern in enterocyte lipid classes. Although most of the radioactivity from all radiolabelled substrates was recovered in triacylglycerols, the amounts of 14C- labelled 16:0, 20:4n-6, 20:5n-3 and 22:6n-3 recovered in the polar lipid fraction were higher than those of 14C-labelled C18 substrates, particularly after 15 min. Conversely, the initial esterification rates of 18:1n-9, 18:2n-6 and 18:3n-3 into triacylglycerols were significantly higher than those of 20:4n-6, 20:5n-3 and 22:6n-3. It is concluded that isolated enterocytes can be used for the study of the mechanism of intestinal fatty acid absorption in fish.     

不同品系球等鞭金藻在生长及脂肪酸组成上的种内差异

球等鞭金藻作为一种优良的单细胞生物饵料具有选育价值。目前我国水产养殖上使用的大多数球等鞭金藻品系都来源于同一株藻株Isochrysis galbana OA-3011,经过多年的地理隔离形成了不同的品系,为了揭示地理隔离对球等鞭金藻表型性状的影响,从而为球等鞭金藻的选育工作提供依据,我们对5个品系的球等鞭金藻(I.galbana FACHB-861,OA3011-QD,OA3011-LK,OA3011-AY和OA3011-LZ)的种属关系、生长和脂肪酸组成进行了比较分析。实验在相同条件下对5个品系球等鞭金藻培养6 d,提取基因组DNA并PCR扩增测定18S rDNA基因序列,每天定时记录各品系的OD_(660),绘制生长曲线,在进入稳定期后收获并进行生物量的测定及脂肪酸的气相色谱分析。18S rDNA基因序列的进化分析表明,OA3011-LK、OA3011-AY和OA3011-LZ具有一致的序列,而OA3011-QD因1个碱基的差异与其他OA3011出现了分离,FACHB-861与OA3011品系亲缘关系较远;对生长和脂肪酸进行对比分析后,结果显示,5个品系的球等鞭金藻在不同的时间达到稳定期,其中FACHB-861表现出了与其他品系明显不同的生长模式。FACHB-861和OA-3011品系的生物量具有显著差异,OA3011-LK、OA3011-AY和OA3011-LZ之间也有显著差异(P0.05),但特异生长率各品系间无显著差异(P0.05)。FACHB-861和OA3011-LZ的总脂肪酸(TFA)含量显著低于OA3011-LK、OA3011-QD和OA3011-AY(P0.05)。FACHB-861的脂肪酸各成分与OA-3011各品系明显不同。然而,在OA-3011各品系间脂肪酸组成也有明显差异,这表明球等鞭金藻存在广泛和频繁的数量性状表型差异。总之,地理隔离造成的这些差异为球等鞭金藻的选育提供了可行性。根据生长速度或DHA、EPA、多不饱和脂肪酸含量等筛选标准初步分析显示,可对OA3011-LK,OA3011-QD进行进一步的选育。