共查询到10条相似文献，搜索用时 50 毫秒
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Md Afsar Ahmed Sumon;Edinson Dante Meregildo-Rodriguez;Po-Tsang Lee;Nguyen Dinh-Hung;Earl T. Larson;Patima Permpoonpattana;Hien Van Doan;Won-Kyo Jung;Nguyen Vu Linh; 《Journal of fish diseases》2024,47(12):e14019
This study provides a comprehensive summary of the findings regarding the application and diagnostic efficacy of droplet digital PCR (ddPCR) in detecting viral and bacterial pathogens in aquaculture. Utilizing a systematic search of four databases up to 6 November 2023, we identified studies where ddPCR was deployed for pathogen detection in aquaculture settings, adhering to Preferred Reporting Items for Systematic Reviews and Meta-analysis of Diagnostic Test Accuracy guidelines. From the collected data, 16 studies retrieved, seven were included in a meta-analysis, encompassing 1121 biological samples from various fish species. The detection limits reported ranged markedly from 0.07 to 34 copies/μL. A direct comparison of the diagnostic performance between ddPCR with quantitative PCR (qPCR) proved challenging due to limited data, thus only a pooled sensitivity analysis was feasible. The results showed a pooled sensitivity of 0.750 (95% confidence interval [CI]: 0.487–0.944) for ddPCR, compared to 0.461 (95% CI: 0.294–0.632) for qPCR, with no statistically significant difference in sensitivity between the two methods (p = .5884). Notably, significant heterogeneity was observed among the studies (I2 = 93%–97%, p < .01), with the year of publication significantly influencing this heterogeneity (p < .001), but not the country of origin (p = .49). No publication bias was detected, and the studies generally exhibited a low risk of bias according to QUADAS-C criteria. While ddPCR and qPCR showed comparable sensitivities in pathogen detection, ddPCR's capability to precisely quantify pathogens without the need for standard curves highlights its potential utility. This characteristic could significantly enhance the accuracy and reliability of pathogen detection in aquaculture. 相似文献
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Abstract Substantial alterations to the legal controls over fish movements have been necessary to implement the Single European Market which came into force on 1 January 1993. This seeks to liberalize trade in fish and fish products whilst avoiding ecological damage, the spread of disease and the commercial damage this may cause. The new regime governing fish movements into and within the European Union under Directive 91/67/EEC, as implemented in England and Wales, is described. Remaining national provisions based on ecological concerns (principally, section 30 of the Salmon and Freshwater Fisheries Act 1975) are examined. Derogations from the principle of freedom of trade within the European Union on environmental protection grounds are assessed, and the interaction between legal restrictions based on pathological and ecological grounds is discussed. 相似文献
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Dibo Liu;David L. Straus;Lars-Flemming Pedersen;Christopher Good;Carlo C. Lazado;Thomas Meinelt; 《Reviews in Aquaculture》2024,16(4):1621-1646
Peracetic acid (PAA) has a long history as an efficacious and eco-friendly disinfectant. It was first synthesised in 1902, and since then a wide range of applications has been developed in various industries. Aquaculture is a more recent industry wherein the potential of PAA is significant. As the global demand for sustainable development increases, there has likewise been growing interest in using PAA in aquaculture as an alternative to less environmentally friendly practices. PAA has no carcinogenic risk to humans (unlike formalin), has negligible harmful by-products (unlike chlorine-based disinfectants) and with appropriate precautions, the risks of causing severe human health damage is easier to control than ozone. Fish show strong physiological recovery and adaptation to PAA, whereas susceptible life stages of pathogens are highly vulnerable, enabling a safe and efficacious disinfection of the entire culture water and not the flow-restricted disinfection by such processes as ultraviolet radiation or ozone. The effective concentration of PAA against many fish pathogens is usually below 2 mg L−1, which is tolerable for most fish, and it has very low environmental risk due to rapid degradation. However, such degradation and the hydrodynamics in production-scale aquaculture systems complicate the practical use of PAA. In this review, we summarise key results of safe concentrations of PAA and its effectiveness specifically for fish farmers. We also outline major difficulties and possible solutions for practical uses of PAA. We intend to bring global attention to this compound and inspire future possibilities for its sustainable use as a water disinfectant in aquaculture. 相似文献
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A quantitative survey of Saprolegnia spp. in the water systems of Norwegian salmon hatcheries was performed. Water samples from 14 salmon hatcheries distributed along the Norwegian coastline were collected during final incubation in the hatcheries. Samples of inlet and effluent water were analyzed to estimate Saprolegnia propagule numbers. Saprolegnia spores were found in all samples at variable abundance. Number of spores retrieved varied from 50 to 3200 L?1 in inlet water and from 30 to >5000 L?1 in effluent water. A significant elevation of spore levels in effluent water compared to inlet water was detected. The estimated spore levels were related to recorded managerial and environmental parameters, and the number of spores in inlet water and temperature was the factor having most influence on the spore concentration in the incubation units (effluent water). Further, the relative impact of spore concentration on hatching rates was investigated by correlation analysis. From this was found that even high spore counts did not impact significantly on hatching success. 相似文献
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Dmrt基因家族是一个与性别决定相关的基因家族。迄今，已在鱼类、爬行类、鸟类、哺乳类等高等动物中检测到了Dmrt基因的存在。为了进一步探讨该家族在系统进化中的保守性，本研究采用简并PCR技术，扩增了罗氏沼虾(Macrobrachium rosenbergii)Dmrt基因的DM结构域。经序列分析，获得了Dmrt基因家族的3个成员，其编码序列分别与人DMRT2、DMRT3、DMRT14基因DM结构域编码序列的相似性分别为93％、80％和95％，根据罗氏沼虾的拉丁名分别命名为MrDmrt2、MrDmrt3、MrDmrt4。与其他动物相关的Dmrt基因进行聚类分析，结果表明，不同进化地位动物的Dmrt基因DM域编码序列存在高度的同源性，显示Dmrt基因在系统进化上高度保守，序列上的相似性可能暗示着它们在功能上的保守性。 相似文献
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