动植物蛋白源替代鱼粉研究进展

With the fast development of aquaculture, fish meal needs increased in recent years, however the quantity of fish catching decreases gradually. Fishmeal is a limited feed resource, and serious concem exists on the future availability of this feedstuff for incorporation in fish diets. Undoubtedly, fish meal is well recognized as the best dietary protein source for most marine carnivorous fishes which required high dietary protein levels compared to omnivorous or herbivorous fish. Fishmeal is known for their high content of essential amino acids and fatty acids, low carbohydrates, high digestibility, low levels of anti-nutritional factors (for fresh fish meal) and is a very good source of minerals and is highly palatable. Thus fish meal is in high demand as the protein source for many formulated diets. However, production of fish meal consumes approximately 35 % of the total global fish catch, and the increasing price and potentially unstable supply in the market could be limiting factors for marine fish culture. There have been strong efforts to define and develop cost-effective protein sources that can, at least partly, substitute for expensive high-quality fish meals in least-cost feed formulations. The search for fish meal substitutes and altemative dietary protein sources is an international research priority that could be of considerable economic advantages. Therefore it is urgent task to find animal and plant protein sources in place of fish meal. Among these, plant feedstuffs have received most attention in recent years, but due to their amino acid unbalances, .presence of anti-nutritional factors and low palatability, a high level of replacement of fish meal with plant feedstuffs in omnivorous fish is generally not well accepted. This paper reviews the research status for other protein sources replacing fish meal based on available information in the literature. Animal and plant protein sources nutrient values are evaluated from the aspect of digestibility, antinutrients, physiological status and suitable supplementation.     

鱼粉在水产饲料中的应用研究

As a main protein source in aquafeeds, fish meal has been extensively studied. Fish sources, freshness, processing temperature, lipid quality and microbiological index are five main aspects of the evaluation of fish meal quality. This paper reviewed the researches on fish meal including the evaluation of fish meal quality, the use of fishmeal and the environmental problems. Biogenic amine is the main potential toxin in decomposed fish meal including mainly histamine, cadaverine, putrescine and tyramine and most studies showed that they could affect the fish growth performance and health. The determination of protein digestibility of fish meal includes pepsin-digestion method, animal test, capillary electrophoresis, etc. The content of phosphorus in fish meal and its utilization can introduce pollution to water bodies and the use of alternative protein and improvement of utilization of fish meal can help to reduce the pollution from fish meal.     

鱼类白细胞介素1β基因的研究概况

Interleukin-1β(IL-1β) is one of the most pleiotropic cytokines and a central regulator of the immune and inflammatory responses. Interleukin-1β was initially discovered within mice and humans and over the last 10 years has been characterized within a wide variety of animals. The IL-1β plays a key role in the inflammatory process, enhancing cell-mediated immunity by inducing the growth and proliferation of lymphocytes, connective tissue cells, and by stimulating immune and inflammatory response effector cells.As an immunoregulatory cytokine, IL-1β has the potential to enhance the immune response induced by a vaccine and/or to modulate the immune response leading to different effector mechanisms. It is produced by many cell types, including monocytes, macrophages,T and B lymphocytes, fibroblasts, and endothelial and epithelial cells. Expression is induced by a diverse range of stimuli, including mitogens, cytokines, and microbial products. There have been considerable evidences provided by biological cross reaction that fish produce IL-1β during immune responses, and the bioactivity of IL-1 in fish has been known for over a decade. But only. since 1999,IL-1β gene has been cloned from the rainbow trout. And from then on, IL-1β gene has been cloned and expressed in many fish confirming that fish produces IL-1β gene during immune responses. In mammals it is produced as an inactive precursor that is processed by interleukin converting enzyme (ICE) to give a biologically active ‘mature‘ peptide. There is no signal peptide in IL-1β and its mechanism is unknown. This is the special part of the gene structure of the IL-1β. And through the program analyzing, we found this special structure in fish IL-1β gene. This paper reviews the functions and structure of gene IL-1β and the studies of the gene IL-1β in fish recently.     

鱼类粘膜免疫研究进展

Fish immunology has achieved great progress in recent years. While before 1990s, most researches focused on the fish systematic immunity, and the mucosal immunity of fish had not been given enough attention. Indeed, it has been shown that fish mucosal immunity plays an important role in disease defense. Fish mucosal immunity research has made some exciting progress in this decade. This review will focus on such progress: Constitution of mucosal-associated tissues and distribution of different immune cells, including T/B lymphocytes, granules, monocytes, macrophages, goblet cells, etc, in these sites have been well described with the development of some monoclonal antibody to these cells and associated techniques. Non-specific immune response mechanism of mucosal tissues reported these years, such as secretion of non-specific anti-bacteria and anti-fungi substances in mucus, the respiratory burst, enzyme activity of immune cells and so on, is believed important for fish disease defense. The specific immunity of mucosal tissues also attracts much interest and makes great achievement in antigen presenting, MHC genes, antibody producing and antibody secreting cells, comparison of serum and mucus immunoglobulin, relationships of immune response between different mucosal immune tissues. Whether mucosal immune system is independent of systematic immune system is another interesting question and causes great concern. In recent years, some evidences from phyletic evolution and ontogenesis show that mucosal immunity is prior to systematic immunity in evolution. Dynamics of antibody producing of mucosal tissues and serum in immersion or oral vaccines immunized fish also shows immune response can be elicited in mucosal tissues independent of systematic immune system. Some researchers also begin to pay attention to factors involved in mucosal immune regulations, for instance, neuromodulators and cytokines. The level of these factors changes in fish immune response process but the mechanisms of regulation still remain unknown. Prospect of the promising future of fish mucosal immunity has also been discussed in this review.     

半滑舌鳎染色体核型分析

The tonguefish Cynoglossus semilaevis (Giinther) is a rare fish species in Chinese offing, inhabiting in the warm water bottom. The metaphase chromosome preparation of the fries has been got from their fins by hot air drying methods, while the metaphase chromosomes of one year old young fish which has physiologically sex differentiation has teen got from their renal tissues by the method of PHA and colchicine injection. The karyotypes were examined. The result shows that there are 42 acrocentric chromosomes in diploid and their karyotype formula is 2n = 42t, and there existed heterotypic sex chromosome which belongs to ZW/ZZ type. The sex ratio in artificial bred stock is nearly 1 : 1.     

水产食品特定腐败菌与货架期的预测和延长

Fresh fish and lightly preserved fish products are welcome by the global market, however, they are also among the most perishable food products. The research on specific spoilage organisms (SSO) reveals the spoilage process of aquatic product. This paper reviews the current knowledge (past ten years) on SSO of fresh fish and lightly preserved fish products with particular emphasis on characteristics of SSO and how to apply this concept to determine, predict and extend the shelf life of aquatic product. During storage, the microflora changes owing to different abilities of the microorganisms to tolerate the preservation conditions. SSO is defined as special microorganisms which can increase rapidly during preservation and has the ability to produce off- odours and off- flavours associated with spoilage, and spoilage metabolites. Identification of an SSO relies on comparison of the sensory and chemical characteristics of spoiled product with those of isolates from the spoilage microflora. Generally, the SSO of fresh fish may be a single species or genus, but the ones of lightly preserved fish products will be more complex. One exciting area for use of SSO aims to obtain quantitative knowledge about probable behavior of SSO and their function during the progression of spoilage. Thus mathematical models on the growth of SSO are established to evaluate the quality lost degree of product, which provide a sound information for the rational development of devices to monitor loss of products shelf life. Models for the growth of Pseudomonas spp, S. putrefaciens, P. phosphoreum have been established, and validated for shelf life prediction of seafood successfully. Another application field of SSO intends to develop the techniques to prolong the shelf life of food products by inhibiting SSO targetedly. Targeted inhibition of spoilage bacteria during preservation reduces their growth and results in a significant extension of shelf life in despite of the activity of non - spoilage organisms has not been influenced. Such techniques have been applied in perversion field of fresh fish and lightly preserved fish.     

韶关市北江水系水利枢纽对鱼类繁殖的影响

The fish spawning ground had been investigated on the Beijiang River near Shaoguan city and the influence on fishes breeding from 7 dam had been estimated.Main result are as follows:the four dam building and designed will result in a big destruction.including disrupt the fish migration(Mengli Dam and ChangshanDam),the fish spawning ground disappear(below Zhangtan Dam).a huge destruction in Ankou fish spawning ground by Changan Dam and a serious of damage on grass lying -egg species will caused by Wantou Dam,especially for catifish Mystus suttatus.On the other hand Zhangtan Dam,Kongiiang Dam,and Menzhou Dam obstructed the fish exchange between upstream and lower stream,however a large water surface became before the dam supported a better condition for fish growth,it seems balance in positive and negative action.The conclusion is that those dam building or designed would be stop or delay in order to maintain fish population and aquatic ecological balance.     

1999年2月黄海声学调查鳀鱼种群群体结构评估

IntroductionEstimation of the size structure of a fish population is often one of the primary objectives of an acoustic/trawl survey (Gunderson 1993;Honkalehto and Williamson 1996 ) .The size structure contains much informa-tion enabling a quick estimate of the fishable size of a stock in a regulated fishery or the recruitment status ofa stock.It is also a common practice to use the number at length of a population together with the age/lengthkey to produce the age structure of the fish popul…     

基于模糊AHP的长寿湖生态系统健康评价

Ecosystem health assessment is a focus and hot spots field of ecological research, there is no unified evaluation indicators and evaluation methods. First, according to the area physical, human features of the Changshou lake and existed research, We established ecosystem health evaluation system which has included three secondary indexes ： the ecological characteristics indicators, the function indicators and the socio - economic indicators, and has 20 indicators. Second, we assess the Changshou lake ecosystem with the ecosystem health index which based on the fuzzy AHP. The conclusions are as follows： in 2006, the Changshou lake ecosystem health index was 0. 471, it is showed that the ecosystem is in sub health, the mainly reasons is its upper reaches of the Di- anjiang county, Liangping county long - term discharge of industrial sewage and pollution of fencing fish.     

鱼类几种新型免疫因子的研究进展

Cytokines are low molecular weight proteins that serve as chemical messengers within the innate and adaptive immune systems. To date, great progresses have been made in fish cytokine researches. A number of cytokine genes have been cloned and sequenced in fish. This review will focus on a number of novel immune-related cytokines including interleukin, interferon, interferon regulatory factors, Myxovirus resistance proteins, transforming growth factor, tumor necrosis factor, chemokines (CC and CXC chemokines), NK dell enhancement factor, MHCⅠ, MHCⅡ and some of their receptors, which have been identified in many fish species recently. Their genes and molecular structures are clarified. These cytokines are evolutionary well conserved. They share high identities at both the nucleotide and amino acid levels with the high vertebrate cytokines, and maintain characteristic structural motifs of those higher vertebrates. The function of some cytokine genes are analyzed in conventional manner by production of recombinant molecules. Several fish cytokines have been identified based on functional similarity to, or cross-reactivity with, mammalian cytokines. Moreover, molecular techniques, such as suppression subtractive hybridization, PCR and cDNA library screening, have recently enabled the identification of fish cytokine genes. Because of fish phylogenetic position and the fact that their immune systems have not been elaborated to the extent seen in mammals, progresses in this field will deepen our understanding of the molecular origins of cytokine genes and extend our knowledge on their mechanisms conferring disease resistance and the recombinant cytokines to control fish diseases.