自主移动式太阳能水车增氧平台设计

针对目前增氧设备不能移动、能源利用单一、自动化及智能化程度低、不具备溶氧检测系统的问题,设计了一种自主移动式太阳能水车增氧平台。通过整体性能的研究分析,设计了增氧平台的整体结构,提出了以超声波测距传感器实时检测和水车左右电机差速控制的自主移动式增氧的实现方法;根据增氧平台工作时间、功率消耗以及阴雨天措施等要求,理论分析了系统太阳能电池板和蓄电池组的配置方法,设计了系统硬件并对各功能模块进行了选型;研究了增氧平台的软件系统以及远程监控端的上位机系统。通过离岸测距实验,验证了增氧平台能够按设定安全距离沿岸边平行移动,并在前方遇到障碍物时进行转向避障,初步实现了增氧平台的自主巡岸移动,扩大了水域的增氧范围。相比传统电力供电固定式增氧设备,增氧平台的增氧范围更大、更灵活,自动化和智能化程度更高。     

一种大水体太阳能自动增氧装置的研发与试验

研发一种大水体太阳能自动增氧装置，为大水体的缺氧、水体污染提供一种解决方法。太阳能自动增氧装置由太阳能光伏发电系统、检测与智能增氧系统、自动化驱动系统组成。光伏发电系统充分利用太阳能资源，解决了电能消耗问题；检测与智能增氧系统实现了增氧过程中氧溶解浓度检测和智能感应运行；自动化驱动系统通过智能感应信号和电子差速控制系统实现增氧机原地转向、转弯和直行3种运动模式的移动，增加了增氧面积。使用太阳能自动增氧装置增氧试验表明，80 min内1 m水深处溶氧量增加0.79 mg/L，2 m水深处溶氧量增加0.78 mg/L，3m水深处溶氧量增加0.77 mg/L，4 m水深处溶氧量增加0.78 mg/L；改善水质试验表明能有有效提高水体溶氧，降低氮磷含量；养殖试验表明，增加鲤产量35.3%、鲢鳙产量31.2%。     

射流式增氧机性能研究

为评价射流式增氧机性能,采用SC/T 6009-1999<增氧机增氧能力试验方法>标准,通过清水试验和养殖池塘试验,研究了射流式增氧机在清水中的增氧能力、动力效率,以及实际养殖池塘中上下水层溶解氧变化.结果表明:射流式增氧机对于下层水体具有良好的增氧效果,能使1.5 m水深处溶氧值提高31.0%;利用产生的水流搅拌水体...     

管道式微孔增氧装置的增氧性能研究

为了评价不同进气量和进气压力对管道式微孔增氧装置增氧性能的影响，参照SC/T 6051—2011《溶氧装置性能试验方法》中的标准测试方法，在3个进气压力条件下开展不同进气量的室内清水增氧试验。结果显示：在相同进气量条件下，降低进气压力可取得较好的增氧性能；在相同进气压力条件下，装置的氧质量转移系数和增氧能力随着进气量增大而增大，氧利用率和动力效率则呈下降趋势，但在进气压力为0.2 MPa时，其氧利用率和动力效率下降幅度较缓。综合考虑增氧能力和运行能耗等因素，装置的进气参数可设置为进气压力0.2 MPa、进气量0.064～0.081m³/h,在该条件下增氧能力达到29.79～34.36 g/h,氧利用率达到29.83%～32.32%,动力效率达到7.67～8.31 kg/(kW·h)。研究表明，进气量对装置的增氧性能有较大的影响，合理控制进气量对于提高增氧性能、降低增氧装置能耗具有重要意义。     

移动式叶轮增氧船的研制

本文介绍了一种新型增氧船的工作原理、结构、主要技术参数、使用及经济效益.该船在增氧技术上选用了动力效率最佳的叶轮式增氧机,通过生产实践表明,把叶轮式增氧机装到船上,移动增氧,技术上是可行的,能满足大面积、高密度水产养殖的需要,具有明显的经济效益和社会效益.     

水车式增氧机性能试验研究

研究了水车式增氧机在清水试验中的增氧能力、动力效率以及实际养殖池塘试验中上下水层溶解氧变化。结果表明,水车式增氧机对于水深为1 m以内的养殖水体具有良好的增氧和搅拌效果,开启100 min左右,可使距增氧机10 m、水深0.9 m处水体溶氧值从6.5 mg/L左右上升到8.7 mg/L左右,和上层水体溶氧值趋于一致;运转时可形成一股较大的定向水流,对鳗鱼等喜好水流的鱼类较为适合。但水车式增氧机对<1.5 m的底层水体增氧作用较弱。本研究为水车式增氧机池塘养殖的运用提供了有益的借鉴。     

典型增氧设备在养殖池塘中组合应用的研究

叶轮式、水车式、射流式和曝气式增氧机是目前我国池塘养殖使用的主要增氧设备;由于结构形式和工作原理的不同,4种形式的增氧机有不同的特点和功能。为提高养殖池塘增氧设备的增氧效果,通过增氧设备对养殖池塘水体不同深度增氧效果的试验和养殖池塘自然增氧的试验,分析了4种典型的增氧设备的增氧性能和特点,提出了叶轮式增氧机与耕水机、水车式增氧机与耕水机、水车式增氧机与射流式增氧机以及曝气增氧机与耕水机组合配置使用的混合增氧模式,可以优势互补,充分发挥各种形式增氧设备功能。通过组合使用,达到对养殖池塘水体最大限度的增氧效果的目的。     

工业化养鱼增氧装置的研制

增氧技术是工业化养鱼中的重要措施.本文介绍的装置主要是在中科院煤化所1982年的科研成果—变压吸附制氧机以及增氧塔、水泵和多孔喷射管等技术的基础上研制而成的.这一装置制出的富氧空气的氧纯度可达80%以上,利用强化溶解方法还可使增氧塔出日的水体溶氧达到20毫克/升以上.在养鱼试验中能保证鱼池水体溶氧在8毫克/升以上.     

锥式增氧装置

中国科学院海洋研究所发明了一种锥式增氧装置，在一密闭容器内向水体增氧，有较高的溶解度，没有气体逸出，并且不需要耗能。     

一种新型管式曝气装置的设计与试验

随着水产养殖业逐渐向高密度、集约化方向的发展,为实现氧气(O_2)高效溶解、二氧化碳(CO_2)调控、流态改善等目的,急需研制新型高效曝气装置,开发高效节能的曝气技术。基于气水两相溶解的双膜理论和文丘里原理,设计开发了一种管式曝气装置,经初步试验获得了影响增氧性能的关键技术参数,并以此为基础对曝气装置进行优化设计,研究新型节能增氧技术。试验结果表明,管式曝气装置中影响曝气效果的因素有水面高程(Hw)、缩径(D_1/D_2)、旋混结构、进气口大小、进气量等。水面高程、缩径、进气口越大,进气量越充足,曝气装置的增氧效果越高;而额外增加延长结构和旋混结构后,曝气装置水头损失增大、进气量下降,从而导致增氧效果显著下降。该装置利用额外水流动力实现增氧,是一种高效、节能、经济的新型曝气装置,可为水产养殖提供一种新的增氧方法。     

Protein and amino acid composition from the mantle of juvenile O ctopus vulgaris exposed to prolonged starvation

Protein and amino acid composition of the mantle of juvenile O ctopus vulgaris (Cuvier, 1797) during fasting for 27 days were determined. Average protein content of octopus mantle was of 711.19 ± 46.80 g kg^?1 DW, and it decreased with increasing fasting days. The non‐essential amino acids content was higher (486.18 ± 11.08 g kg^?1 protein) than essential amino acids (425.82 ± 9.15 g kg^?1 protein) at the start of the experiment (unstarved animals). The results suggest that the amino acid profile of the mantle where the most abundant amino acids are Arg, His, Lys, Gly, Leu and Pro could indicate a prolonged fasting condition (>20 days) or poor nutrition of O . vulgaris. This study supports the idea of using mantle for metabolic needs of starved O . vulgaris suggesting that the degradation pathway of amino acids to pyruvate and tricarboxylic acid cycle intermediates was favoured contrary to the degradation pathway of ketogenic amino acids. Special considerations should be taken concerning Thr, Ile, Ser, Ala, Asx (Asp, Asn), Glx (Glu, Gln) (because of their fast intake) and Lys and His (due to their stable contents) during a prolonged period of fasting.     

美人鱼发光杆菌对凡纳滨对虾非特异性 免疫功能及抗病力的影响

在基础饲料中分别添加0.1%和1%美人鱼发光杆菌灭活菌、0.1%美人鱼发光杆菌活菌配制成3种免疫实验饲料,以基础饲料为空白对照组饲料,每组设3个平行样。对个体质量为(4.83±0.36)g的凡纳滨对虾进行为期20 d的饲养实验,分别在0、5、10、15和20d进行取样,以血清中的酚氧化酶(PO)、酸性磷酸酶(ACP)、碱性磷酸酶(AKP)、超氧化物歧化酶(SOD)和溶菌酶(UL)活性为免疫指标,探讨了美人鱼发光杆菌作为免疫制剂对凡纳滨对虾非特异性免疫效应的影响;在投喂免疫饲料后的第22天,按0.004 2 kg/kg体重的剂量,直接投喂对虾白斑综合征病毒(WSSV)病料,并记录累积死亡率。结果表明,美人鱼发光杆菌免疫实验组对凡纳滨对虾血清中PO、ACP、AKP、UL和SOD活性影响明显高于对照组,并且在饲料中添加美人鱼发光杆菌后,明显提高了对虾抵御WSSV感染的能力。其中0.1%美人鱼发光杆菌活菌实验组的抗病毒感染能力最强,WSSV感染14d内累计死亡率为63.3%±5.8%;而对照组为96.7%±3.3%。研究表明,美人鱼发光杆菌添加在对虾饲料中能提高凡纳滨对虾非特异性免疫水平,增强抵抗疾病的能力,将其作为对虾免疫增强剂具有良好的应用前景。     

Effects of Spirulina platensis as a feed additive on growth and coloration of blue dolphin cichlids (Cyrtocara moorii Boulunger, 1902)

This study was carried out to investigate the effects of replacing fish meal with dietary Spirulina as a feed supplement on the growth performance and coloration of blue dolphin cichlids (Cyrtocara moorii). Five isonitrogenous (47% crude protein) and isocaloric (17.36 kJ/g digestible energy) diets were for formulated to replace FM with 0, 5, 10, 15 and 20% Spirulina (designated as Control, SP5, SP10, SP15 and SP20 respectively) and fed to the fish (initial body weight, 3.15 ± 0.01 g). Fish were randomly distributed into fifteen 120 L aquariums (26.5 ± 1.00°C), 15 fish per aquarium. The diets were tested in triplicate for 12 weeks. Experimental groups were fed twice daily (09:00 and 17:00) by hand to satiation. At the end of the feeding trial, significantly (p < 0.05) higher weight gain (WG), specific growth rate (SGR), protein efficiency ratio (PER) and lower feed conversion ratio (FCR) were observed in fish fed the SP10 diet when compared to the SP20 diet. There was no significant difference in these parameters between the other groups. The skin coloration of blue dolphin cichlid fed a diet containing Spirulina meal was enhanced. The best coloration was observed in the SP15 group. These impressions were objectively validated by chemical determinations of carotenoids extracted from fish skins and passed statistical tests of significance. The study findings show that Spirulina meal does not diminish growth rates except at very high levels.     

A Minchinia mercenariae‐like parasite infects cockles Cerastoderma edule in Galicia (NW Spain)

The cockle Cerastoderma edule fishery has traditionally been the most important shellfish species in terms of biomass in Galicia (NW Spain). In the course of a survey of the histopathological conditions affecting this species in the Ria of Arousa, a haplosporidan parasite that had not been observed in Galicia was detected in one of the most productive cockle beds of Galicia. Uni‐ and binucleate cells and multinucleate plasmodia were observed in the connective tissue mainly in the digestive area, gills and gonad. The parasite showed low prevalence, and it was not associated with abnormal cockle mortality. Molecular identification showed that this parasite was closely related to the haplosporidan Minchinia mercenariae that had been reported infecting hard clams Mercenaria mercenaria from the Atlantic coast of the United States. The molecular characterization of its SSU rDNA region allowed obtaining a fragment of 1,796 bp showing 98% homology with M. mercenariae parasite. Phylogenetic analysis supported this identification as this parasite was clustered in the same clade as M. mercenariae from the United States and other M. mercenariae‐like sequences from the UK, with bootstrap value of 99%. The occurrence of M. mercenariae‐like parasites infecting molluscs outside the United States is confirmed.     

Dietary thiamin and pyridoxine requirements of fingerling Indian major carp,Cirrhinus mrigala (Hamilton)

Two experiments were conducted to quantify the dietary thiamin (experiment I) and pyridoxine (experiment II) requirements of fingerling Cirrhinus mrigala for 16 weeks. In experiment I, dietary thiamin requirement was determined by feeding seven casein–gelatin‐based diets (400 g kg^?1 CP; 18.69 kJ g^?1 GE) with graded levels of thiamin (0, 0.5, 1, 2, 4, 8 and 16 mg kg^?1 diet) to triplicate groups of fish (6.15 ± 0.37 cm; 1.89 ± 0.12 g). Fish fed diet with 2 mg kg^?1 thiamin had highest specific growth rate (SGR), protein retention (PR), RNA/DNA ratio, haemoglobin (Hb), haematocrit (Hct), RBCs and best feed conversion ratio (FCR). However, highest liver thiamin concentration was recorded in fish fed 4 mg thiamin kg^?1 diet. Broken‐line analysis of SGR, PR and liver thiamin concentrations exhibited the thiamin requirement in the range of 1.79–3.34 mg kg^?1 diet (0.096–0.179 μg thiamin kJ^?1 gross energy). In experiment II, six casein–gelatin‐based diets (400 g kg^?1 CP; 18.69 kJ g^?1 GE) containing graded levels of pyridoxine (0, 2, 4, 6, 8 and 10 mg kg^?1 diet) were fed to triplicate groups of fish (6.35 ± 0.37 cm; 1.97 ± 0.12 g). Fish fed diet containing 6 mg kg^?1 pyridoxine showed best SGR, FCR, PR, RNA/DNA ratio, Hb, Hct and RBCs, whereas maximum liver pyridoxine concentration was recorded in fish fed 8 mg kg^?1 dietary pyridoxine. Broken‐line analysis of SGR, PR and liver pyridoxine concentrations reflected the pyridoxine requirement from 5.63 to 8.61 mg kg^?1 diet. Data generated during this study would be useful in formulating thiamin‐ and pyridoxine‐balanced feeds for the intensive culture of this fish.     

Sea bream culture in Egypt; status, constraints and potential

Marine fish farming in Egypt began in 1976 with the culture of gilthead sea bream, (Sparus aurata) as this fish was notably adaptable to brackish and marine pond conditions. Today, marine fish and shrimp farms amount to about 19,000 ha, out of which 42% is already in production while the rest, i.e., 58% is still under construction. In 1997, cultured gilthead sea bream production of 2,250 tons made up 3% of the 75,000 tons total aquaculture catch. In polyculture, usually with the grey mullet and sea bass, gilthead sea bream contributed 440 kg ha^–1 to the total yield of 1,700 kg ha^–1 (26%) over a period of 16 months. For the same period, the yield of monoculture ponds averaged 100 kg ha^–1, while in marine cages, yields ranged from 4–10 kg m³. In 1996–1997, fry of 0.25–1 g and fingerlings 1–10 g with a total of 3 million, were collected from the wild and 1 million fry were produced in the three marine hatcheries out of the four existing ones. The development of sea bream culture in Egypt is now severely inhibited by the shortage of seeds and adequate feeds. Exports of both sea bream and sea bass, during 1994–1996 averaged 1,300 tons per year.     

Fertilization by refrigerator stored sperm of the Indian major carp, Labeo calbasu (Hamilton, 1822)

This study reports on the spermatological properties, and on the development of a protocol for refrigerator storage (4°C) of Labeo calbasu (Hamilton, 1822) sperm for artificial breeding. Volume, motility, concentration and pH of the freshly collected sperm were 2.21 ± 0.53 (μL g^?1 of fish weight) (mean ± SD), 95 ± 1 (%), 1.93 ± 0.44 × 10⁹ (cells mL^?1) and 7.56 ± 0.17 respectively. Sperm activation was evaluated at different osmolalities of NaCl solution, and motility ceased completely when osmolality of the extender was ≥287 mOsmol kg^?1. Sperm retained motility for 24, 72 and 108 h, after refrigerator storage when sperm were undiluted, suspended in Alsever's solution and suspended in Alsever's solution containing 5% methanol respectively. Fertilization rate of fresh eggs with sperm stored at 4°C in Alsever's solution and Alsever's solution containing 5% methanol was 77% and 60% with a hatching rate of 60% and 43% respectively. The fertilization and hatching success of the stored sperm suggests potential to use refrigeration for transporting genetic material to hatcheries for artificial breeding of L. calbasu in Bangladesh.     

A novel approach to denitrification processes in a zero-discharge recirculating system for small-scale urban aquaculture

This paper presents an innovative process to solve the nitrate build-up problem in recirculating aquaculture systems (RAS). The novel aspects of the process lie in a denitrification bioreactor system that uses solid cotton wool as the primary carbon source and a unique degassing chamber. In the latter, the water is physically stripped of dissolved gaseous O₂ (by means of a Venturi vacuum tube), and the subsequent denitrification becomes more efficient due to elimination of the problems of oxygen inhibition of denitrification and aerobic consumption of cotton wool. The cotton wool medium also serves as a physical barrier that traps organic particles, which, in turn, act as an additional carbon source for denitrification. Operation in the proposed system gives an extremely low C/N ratio of 0.82 g of cotton wool/g of nitrate N, which contributes to a significant reduction of biofilter volume. The additional advantage of using solid cotton wool as the carbon source is that it does not release organic residuals into the liquid to be recycled. Operation of the system over a long period consistently produced effluents with low nitrate levels (below 10 mg N/l), and there was only a very small need to replace system water. The overall treatment scheme, also incorporating an aerobic nitrification biofilter and a granular filtration device, produced water of excellent quality, i.e., with near-zero levels of nitrite and ammonia, a sufficiently high pH for aquaculture, and low turbidity. The proposed system thus provides a solution for sustainable small-scale, urban aquaculture operation with a very high recovery of water (over 99%) and minimal waste disposal.     

Effects of metomidate anaesthesia or transfer to pur sea water on plasma parameters in ammonia-exposed Atlantic salmon (Salmo salar L) in sea water

Atlantic salmon (Salmo salar L) postsmolts weighing 150 ± 53 g were exposed to 14–15 mg l^–1 TA-N (total ammonia-N) in sea water in 1 m³ tanks for 24h. Blood samples were then taken A) immediately after the fish were netted from the exposure tanks and stunned by a blow to the head; B) 2–20 min after the fish were transferred to 15 l of an anaesthetic solution of metomidate in ammonia-free sea water; or C) 2–20 min after the fish were transferred to 15 l of ammonia-free sea water. Plasma TA-N level was 18% lower in the anaesthetised fish compared to in the fish sampled directly from the exposure tanks (p 0.05), and accordingly 16% lower in the fish transferred to pure sea water although this difference was not significant (p = 0.07). Plasma glucose level was higher in the fish transferred to pure sea water than in the fish receiving the two other treatments (p 0.05), but plasma urea, osmolality, Na^{+, Cl–, Ca2+ or Mg2+} levels did not vary significantly between the different treatments. Plasma TA-N level increased with time in the fish in the metomidate solution (p 0.02).