闽东渔场不同生态类群的鱼类资源生产量

以历次海洋科学调查在闽东渔场获得的初级生产力为基础,通过2000~2001年对该渔场的鱼类资源结构、浮游植物有机碳含量、生态效率、主要经济鱼类营养级及其有机碳含量等模型参数的调查和检测,采用营养动态模型和Cushing模型估算鱼类资源的生态容量(潜在生产量),进而应用Steele模型分别估算中上层鱼类、底层鱼类的资源生产量,最后从鱼类资源生产量中分离出近底层鱼类的资源生产量。采用Gadima模式估算鱼类及其各生态类群资源的最大可持续开发量,并讨论它们的开发利用程度。估算结果:闽东渔场鱼类资源生产量为63.08×104t,最大可持续开发量为33.82×104t。其中中上层鱼类资源生产量为38.68×104t,最大可持续开发量为20.34×104t,底层和近底层鱼类资源生产量分别为13.22×104t和11.17×104t,最大可持续开发量合计为15.50×104t。底层和近底层鱼类实际年渔获量自1993年以来已连续11年超过其最大可持续开发量,呈过度捕捞。中上层鱼类实际年渔获量也于1998年以来连续6年超过其最大可持续开发量。因此必须加强保护和管理,采取有力措施削减捕捞力量和渔获量,实行捕捞力量和渔获量“负增长”,待到渔获量下降到最大可持续开发量水平时,才恢复实施“零增长”制度。     

闽南-台湾浅滩渔场鱼类及其不同生态类群的资源生产量

以闽南-台湾浅滩渔场历次海洋科学调查获得的初级生产力为基础,在对该渔场的鱼类资源结构调查、浮游植物有机碳含量检测和生态效率的测算、52种主要经济鱼类的营养级及其有机碳含量的检测后,先采用营养动态模型和Cusing模型估算鱼类资源的生态容量(潜在生产量),然后应用Seetile模型分别估算中上层鱼类、底层鱼类的资源生产量,进而从鱼类资源生产量中分离出近底层鱼类的资源生产量,采用Gadima模式和MSY简单模式估算各生态类群的鱼类资源最大可持续开发量,并讨论它们的开发利用程度.估算结果:鱼类资源生态容量为98.62×104 t,最大持续可开发量为48.34×104 t.其中中上层鱼类资源生产量为56.90×104 t,最大持续可开发量为28.35×104 t,底层和近底层鱼类资源生产量分别为19.45×104 t和22.27×104 t,合计最大持续可开发量为21.27×104 t.底层和近底层鱼类实际年渔获量自1994年以来已连续9年超过其最大持续可开发量,呈过度捕捞.中上层鱼类实际年渔获量至今尚未超过其最大可持续开发量.     

闽中渔场不同生态类群的鱼类资源生产量

本文以海洋生态系统营养动力学为理论依据,依据台湾海峡历次海洋科学调查在闽中渔场所获得的初级生产力为基础,通过对该渔场的浮游植物有机碳含量和生态效率及61种主要鱼类的营养级,58种主要鱼类有机碳含量的调查、检测。采用营养动态模型和Cushing模型估算鱼类资源潜在生产量,同时采用Steele模式估算中上层鱼类和底层鱼类资源生产量,进而从鱼类生产量中分离出近底层鱼类资源的生产量;采用Cadima模式估算鱼类及其不同生态类群的最大可持续开发量。估算结果:营养动态模型和Cushing模型估算鱼类资源潜在生产量分别为38.52×104t和44.34×104t,平均41.43×104t;Steele模式估算中上层鱼类和底层鱼类资源生产量分别为25.31×104t和8.05×104t,近底层鱼类资源生产量为8.07×104t;Cadima模式估算鱼类最大可持续开发量为25.41×104t,中上层鱼类最大可持续开发量为13.96×104t,底层和近底层鱼类最大持续可开发量为9.51×104t;1994年以来鱼类实际年渔获量在27.00×104t~40.01×104t,已连续11年超过了鱼类资源最大可持续开发量。1994年以来投入的捕捞力量为3702艘~5967艘福建标准机动单拖渔船,也连续11年超过了Schaefer和Fox2种剩余产量模式估算的最大持续捕捞力量3678艘福建标准机动单拖渔船,呈现捕获量和捕捞力量“双超”局面。底层和近底层鱼类渔获量自1985以来连续19年超过其最大可持续开发量;中上层鱼类渔获量1998年以来连续6年超过其最大可持续开发量;鱼类的主要种群结构出现简单化、小型化和低龄化,生态学参数渐近体长L∞和渐近体重W∞趋小,个体生长曲率K加大,体重生长拐点tr提前,初届性成熟体长趋小,捕捞死亡系数提高和开发比率上升,资源明显衰退。必须加强对该渔场鱼类资源的管理,采取有力措施控制捕捞力量的投入和渔获量的产出,以防鱼类资源进一步恶化。     

闽东渔场鱼类资源生态容量和最大可持续开发量

以海洋生态系统营养动力学为理论依据,根据历次调查所获得的闽东渔场初级生产力资料,通过对该渔场浮游植物有机碳含量和生态效率的测算、52种主要鱼类营养级及其有机碳含量的检测,采用营养动态模型和Cushing模型估算鱼类资源生态容量(自然生产量),采用Cadima模式和MSY简单模式估算鱼类资源最大可持续开发量。估算结果:闽东渔场鱼类资源生态容量平均为6.308×10~5t,最大可持续开发量为3.268×10~5t。1994年以来闽东渔场实际年渔获量3.435～4.685×10~5t,平均4.267×10~5t,已连续9年超过鱼类资源最大可持续开发量,呈现过度捕捞态势。必须实行渔获量负增长制度才能使鱼类资源得到有效恢复。     

闽中渔场鱼类资源生产量和最大可持续开发量

以海洋生态系统营养动力学为理论依据,依据历次台湾海峡海洋科学调查在闽中渔场所获得的初级生产力为基础,通过2000~2001年对该渔场的浮游植物有机碳含量和生态效率及61种主要经济鱼类的营养级,58种主要鱼类有机碳含量的调查、检测,采用营养动态模型和Cushing模型估算鱼类资源生产量;进而采用Cadima模式和剩余产量模式估算鱼类资源最大可持续开发量。估算结果:营养动态模型估算鱼类资源潜在生产量为38·59×104t,Cushing模型估算为43·68×104t;Cadima模式估算最大可持续开发量为25·32×104t;Schaefer和Fox模式估算最大可持续开发量分别为23·96×104和25·28×104t,估算的最大持续捕捞力量换算为以福建单拖渔船艘为标准分别为3372和3983艘;1994年以来实际年渔获量在27·00×104~40·01×104t,实际捕捞力量在3897~5967艘标准单拖渔船,已连续11年超过了鱼类资源最大可持续开发量和鱼类资源可承载的最大持续捕捞力量。主要鱼类种群结构出现简单化、小型化和低龄化,生态学参数渐近体长L∞和渐近体重W∞趋小,个体生长速率K加大,体重生长拐点tr提前,初次性成熟年龄提早,捕捞死亡系数提高和开发比率上升,资源明显衰退。必须加强对该渔场鱼类资源的管理,采取有力措施控制捕捞力量的投入和渔获量的产出,以防鱼类资源进一步恶化。     

闽南—台湾浅滩渔场鲐鲹鱼类群聚资源生产量和允许总渔获量

以海洋生态系营养动力学为基础,采用Steele模式估算闽南—台湾浅滩渔场中上层鱼类资源年生产量为86.14×10~4t,进而以鲐鲹鱼类群聚资源在中上层鱼类资源的比例,估算鲐鲹鱼类群聚资源的年生产量为51.76×10~4t。Gulland和MSY简单模式估算鲐鲹鱼类群聚资源的最大持续产量分别为25.00×10~4t和25.88×10~4t。Schaefer和Fox剩余产量模式估算鲐鲹鱼类群聚资源的最大持续产量分别23.60×10~4t和23.41×10~4t,最大持续捕捞力量分别为1140.41×10~4kw·d和1266.66×10~4kw·d。折算为福建灯光围网渔船分别为424和471组,并从开发现状和种群生态学及捕捞死亡等参数分析鲐鲹鱼类群聚资源的开发前景。     

闽南、台湾浅滩渔场主要捕捞作业数量配置

根据评估的闽南、台湾浅滩渔场渔业资源生态容量121.33×104t和最大持续产量63.32×104t及闽南、台湾浅滩渔场不同类群渔业资源的开发利用现状,用单位捕捞力量渔获量和平均单船功率配置福建省在闽南、台湾浅滩渔场主要捕捞作业的功率和渔船数量,以达到渔业资源的合理利用和养护,促进闽南、台湾浅滩渔场的可持续发展。     

汕头～台湾浅滩生态系统渔业资源生产量及最大持续产量评估

本文以汕头～台湾浅滩海域的初级生产力为基础,以主要鱼类品种的营养级及其有机碳含量和生态效率,运用营养动态模型和Cushing模型估算海域生态系统渔业资源生产量,同时,运用Gulland模式和MSY简单模式估算渔业资源最大可持续开发量,结果表明该海域渔业资源生产量为77.4万吨,最大可持续开发量为45.6万吨,近十年来全渔场实际年捕捞量在55万吨以上,超过了渔业资源的最大持续产量,处于过度捕捞状态。     

台湾海峡及邻近海域渔业资源可持续开发量研究

利用1999～2001年课题组在台湾海峡及邻近海域采集的海洋渔业资源生物资料和在岸上采集的生产技术资料,通过分析渔业资源种类组成和结构、测算初级生产力,检测浮游植物含碳率,鱼类、虾蟹类、头足类等主要种的营养级及其含碳量率、测算生态效率;利用Cushing模型和营养动态模型估算该海区的潜在渔业资源量,利用Cadima经验公式和Gulland经验公式估算渔业资源最大持续开发量。结果表明,该海区的潜在渔业资源量为250.34×104t,最大可持续开发量为136.98×104t。2000年该海区的实际年捕捞量为217.2×104t,超过了渔业资源的剩余产量80.22×104t。因此,为了持续利用该海区的渔业资源,应减少投入捕捞力量和实行捕捞限额制度。     

汕头～台湾浅滩渔场中上层鱼类资源量 鲐黪鱼类群聚资源量及最大持续产量评估

本文以汕头～台湾浅滩海域生态系统渔业资源容纳量、初级生产力和资源生物含碳率及其转化为基础，根据鲐黪鱼类体内含碳率的测定结果，估算渔场中上层鱼类资源量和鲐黪鱼类群聚资源量，并运用Gulland模式和MSY简单模式估算渔业资源最大可持续开发量。结果显示：渔场中上层鱼类资源量为33．75万吨、最大持续产量16．875万吨：渔场鲐黪鱼类群聚资源量20．25万吨、最大持续产量10．2万吨。近十年来全渔场实际年捕捞量在5．5—10万吨之间，中上层鱼类资源接近充分利用。     

Association between the interannual variation in the oceanic environment and catch rates of bigeye tuna (Thunnus obesus) in the Atlantic Ocean

The environmental processes associated with variability in the catch rates of bigeye tuna in the Atlantic Ocean are largely unexplored. This study used generalized additive models (GAMs) fitted to Taiwanese longline fishery data from 1990 to 2009 and investigated the association between environmental variables and catch rates to identify the processes influencing bigeye tuna distribution in the Atlantic Ocean. The present findings reveal that the year (temporal factor), latitude and longitude (spatial factors), and major regular longline target species of albacore catches are significant for the standardization of bigeye tuna catch rates in the Atlantic Ocean. The standardized catch rates and distribution of bigeye tuna were found to be related to environmental and climatic variation. The model selection processes showed that the selected GAMs explained 70% of the cumulative deviance in the entire Atlantic Ocean. Regarding environmental factors, the depth of the 20 degree isotherm (D20) substantially contributed to the explained deviance; other important factors were sea surface temperature (SST) and sea surface height deviation (SSHD). The potential fishing grounds were observed with SSTs of 22–28°C, a D20 shallower than 150 m and negative SSHDs in the Atlantic Ocean. The higher predicted catch rates were increased in the positive northern tropical Atlantic and negative North Atlantic Oscillation events with a higher SST and shallow D20, suggesting that climatic oscillations affect the population abundance and distribution of bigeye tuna.     

Growth performance,digestive enzyme activity and immune response of Japanese sea bass,Lateolabrax japonicus fed with fructooligosaccharide

In this experiment, a feeding trial was performed to determine the effects of fructooligosaccharide (FOS) on growth performance, digestive enzyme activity and immune response of Japanese sea bass, Lateolabrax japonicus juveniles (initial weight 38.3 ± 0.5 g), and the fish were examined following feeding with six levels of FOS (0, 0.5, 1, 2, 4 and 6 g/kg) for 28 days. Significant enhancement of weight gain (WG) and specific growth rate (SGR) was found in fish fed 1 g/kg FOS incorporated diets (p < .05), while the feed conversion ratio (FCR) in the 1, 2 g/kg FOS groups reduced significantly compared with the control (p < .05). Besides, the crude lipid in the 4, 6 g/kg FOS groups increased significantly compared with the control (p < .05). On the other hand, the erepsin and lipase activities significantly elevated in intestine of fish fed 2 g/kg FOS (p < .05) and the lysozyme activity in serum of fish fed 2 g/kg FOS were significantly higher than that in the control (p < .05). Moreover, the alkaline phosphatase activities in serum of fish fed 0.5, 1, 2 g/kg FOS were significantly higher than in control (p < .05). Regression analysis showed that the relationships between dietary FOS levels and either SGR, FCR, erepsin or lysozyme activities were best expressed by regression equations, and the optimal inclusion levels are 1.37, 1.80, 3.06, 3.11, 1.93 and 1.80 g/kg for SGR, FCR, erepsin, lipase, lysozyme and total superoxide dismutase activities, respectively. Overall, this study revealed that FOS incorporated diets could beneficial for L. japonicus culture in terms of increasing the growth, digestion and immune activities. Under the present experimental condition, the optimal supplementary level of FOS in the diet of L. japonicus is 1–3 g/kg.     

Involvement of gonadal steroids in final oocyte maturation of white perch (Morone americana) and white bass (M. chrysops): in vivo and in vitro studies

Plasma estradiol-17 (E₂), testosterone (T), 17,20-dihydroxy-4-pregnen-3-one (DHP) and 17,20,21-tri-hydroxy-4-pregnen-3-one (20-S) levels were measured by radioimmunoassay (RIA) in white perch (Morone americana) and white bass (M. chrysops) that were induced to undergo final oocyte maturation (FOM) with human chorionic gonadotropin (hCG). Plasma DHP levels increased in females of both species in association with oocyte germinal vesicle migration (GVM) and germinal vesicle breakdown (GVBD) and decreased thereafter. Plasma 20-S levels also increased with oocyte GVM in white bass, but were several-fold lower than DHP levels. Circulating E₂ and T levels were greatest during GVM and GVBD in both species and decreased to low levels during oocyte hydration and ovulation. Follicles from white perch and white bass which received a priming injection of hCG in vivo, produced both DHP and 20-S in vitro after exposure to hCG and their oocytes underwent GVBD. Ovarian incubates from unprimed fish of either species produced only E₂ and T and their oocytes did not complete GVBD. Oocytes from unprimed bass, but not perch, matured when follicles were exposed to hCG in vitro. Both trilostane and cycloheximide blocked in vitro production of DHP and 20-S and oocyte GVBD by white perch follices. DHP and 20-S were equipotent inducers of FOM in the GVBD bioassay. None of several other structurally-related steroids tested were effective within a physiological range of concentrations. These results indicate a role for DHP and 20-S in the control of FOM in white perch and white bass.     

Cardiac,ventilatory and metabolic responses of two ecologically dissimilar species of fish to waterborne cyanide

Changes in heart rate, ventilatory activity and oxygen consumption were determined in trout (Salmo gairdneri) and brown bullhead catfish (Ictalurus nebulosus) during exposure to a steadily increasing concentration of waterborne cyanide selected to produce death in 8–9 hours for each species. The lethal cyanide concentration for the bullheads was an order of magnitude higher than for trout. Trout developed an immediate and gradually increasing bradycardia throughout the exposure period. Cyanide produced tachycardia in the bullhead followed by a gradual onset of bradycardia as the concentration of cyanide was raised. Pericardial injection of atropine (a muscarinic cholinergic antagonist) indicated that bradycardia in the trout was due initially to increased vagal tone but later due to the direct effect of cyanide on the heart. Hyperventilation in the trout persisted throughout the exposure period, although the rate and amplitude fluctuated and was variable between individual fish. During the last hour of exposure (highest cyanide concentration), ventilation was characterized by rapid, shallow breaths followed by a sudden respiratory arrest. The bullheads exhibited hyperventilation during the first 3 hours of exposure followed by a gradual, linear drop in ventilation rate and amplitude until death occurred. Cardiac and ventilatory responses in both species were attributed to stimulation of central and peripheral chemoreceptors by cyanide. Evidence is presented which suggests the initial response in the bullheads was due, at least in part, to gustatory stimulation by the cyanide. Oxygen consumption of the trout remained above pre-exposure levels for the majority of the test period. Oxygen consumption in the bullhead paralleled the changes in heart and ventilatory rates. Whole-body lactate levels of fingerlings of both species during cyanide exposure were measured to estimate the extent of anaerobiosis. Whole-body lactate levels were much greater in the bullheads than the trout, indicating a higher capacity for anaerobiosis, possibly due to a greater fuel supply. Overall, the trout responded to cyanide in a manner similar to that produced by environmental hypoxia whereas the bullheads experienced a gustatory stimulus which masked the hypoxia-like response.     

An overview of stress physiology of fish transport: changes in water quality as a function of transport duration

This study brings an integrated analysis about the relationship between water deterioration and its physiological consequences in live fish transport. The analysis was focused on the transport water and its deterioration, and physiological challenges imposed on the fish. Usual commercial handling procedures employed to mitigate fish stress during transport were discussed. Future topics of research for the establishment of safer fish transport protocols were proposed. Transport was classified into short (≤8 h) or long transport (>8 h). The main issue in short transports should be the prevention of water pH reduction, while in long transports it is the increase in ammonia. Plasma cortisol is the most employed marker for stress and is acutely elevated upon short episodes of transport, but remains elevated even in long‐transport events. Plasma glucose is perhaps a better marker for handling stress. Plasma lactate, pH, osmolality CO₂ and ions should be more often evaluated. Plasma Na⁺ and Cl^‐ are very useful markers of acidosis, due to their respective exchange for H⁺ and , for acid–base regulation. The establishment of species‐specific transport protocols should be preceded by such combined analyses of water and physiological parameters.     

Are hatchery‐reared abalone naïve of predators? Comparing the behaviours of wild and hatchery‐reared northern abalone,Haliotis kamtschatkana (Jonas, 1845)

Abalone populations have declined worldwide, generating interest in enhancement using hatchery‐reared individuals. In many cases, such restoration efforts have met with limited success due to high predator‐induced mortality rates. Furthermore, the mortality rates of outplanted hatchery abalone are often considerably higher than for wild individuals. This study uses northern abalone (Haliotis kamtschatkana) as a case study to determine whether hatchery‐reared abalone behave differently than their wild counterparts. In the field, outplanted hatchery‐reared abalone were significantly less responsive than wild abalone, in terms of number of abalone responding and intensity of response, to nearby movement and to physical contact with an inert probe. Also, when encountering a cue to which all abalone responded (a seastar predator), hatchery‐reared individuals remained subdued. Anti‐predator behavioural deficits in hatchery‐reared abalone were more pronounced in 4‐year‐old individuals than in 1‐year‐old individuals, suggesting an influence of either age or amount of time spent in the hatchery environment. These behavioural differences are expected to increase the vulnerability of hatchery‐reared abalone to predators, and are likely a major cause of their elevated predator‐induced mortality when outplanted.     

Effects of cadmium on the kinetics of calcium uptake in developing tilapia larvae, Oreochromis mossambicus

The toxic effects of Cd²⁺ on Ca²⁺ influx kinetics in developing tilapia (Oreochromis mossambicus) larvae were evaluated. Addition of 20 µg l^-1 of Cd²⁺ to the environment of 0 and 3 day-old larvae competitively inhibited the Ca²⁺ uptake within 4h resulting in a great increase in K_m values for Ca²⁺ influx (19.3 and 17.4 fold, respectively) as compared with their respective controls. Consequently, the actual Ca²⁺ influx of larvae in solutions of 0.2 mM Ca²⁺ are suppressed by 32–45%. Also, 3 day-old larvae were more sensitive to internally accumulated Cd²⁺ than 0 day-old larvae. Although the Ca²⁺ influx in 0 and 3 day-old larvae may be restored to the levels of their respective controls with 24h of being transferred to a 20 µg l^-1 Cd²⁺ solution, total body Ca²⁺ content was significantly reduced in 3 day-old larvae. Increased Ca²⁺ uptake efficiency ensures sufficient Ca²⁺ for normal growth. However, rapid increase in Ca²⁺ influx after hatching also leads to higher Cd²⁺ uptake. Exposure to Cd²⁺ will lead to a drop in body Ca²⁺ content resulting in retardation of larval growth. Therefore, we conclude that if Ca²⁺ uptake is interfered with at this critical stage of development, larvae will not be able to maintain normal levels of body Ca²⁺ and will show signs of Cd²⁺ poisoning.     

Migratory dynamics of stream-spawning longnose gar (Lepisosteus osseus)

Abstract– Literature evidence suggests that lake-dwelling longnose gar (Lepisosteus osseus) enter tributary streams to spawn, Until the present study, the dynamics of this breeding migration had never been investigated quantitatively. During the summers of 1991 and 1992, longnose gar were captured as they entered Weaubleau Creek, Missouri, a tributary of Harry S. Truman Reservoir. The in-stream spawning migration began in early April and ended in late May, and was positively correlated with stream flow and water level, and negatively correlated with water temperature. In-stream residence times ranged from 15 to 94 days, with males exhibiting longer residence times than females. Once in-stream, longnose gar travelled as far as 10 km upstream and occupied certain pools at greater relative frequencies. Although the reason for this preferential utilization is not completely understood, it may relate to pool depth and riffle proximity. Longnose gar disperse from the spawning stream great distances, with gar captured in Weaubleau Creek being recaptured up to 48 km away. This information should provide fisheries biologists the means to consider the reproductive ecology of this species in their conservation and management decisions.     

Nutritional regulation of hepatocyte fatty acid desaturation and polyunsaturated fatty acid composition in zebrafish (Danio rerio) and tilapia (Oreochromis niloticus)

The desaturation and elongation of [1-¹⁴C]18:3n-3 was investigated in hepatocytes of the tropical warm freshwater species, zebrafish (Danio rerio) and Nile tilapia (Oreochromis niloticus). The hepatocyte fatty acid desaturation/elongation pathway was assayed before and after the fish were fed two experimental diets, a control diet containing fish oil (FO) and a diet containing vegetable oil (VO; a blend of olive, linseed and high oleic acid sunflower oils) for 10 weeks. The VO diet was formulated to provide 1% each of 18:2n-6 and 18:3n-3, and so satisfy the possible EFA requirements of zebrafish and tilapia. At the end of the dietary trial, the lipid and fatty acid composition was determined in whole zebrafish, and liver, white muscle and brain of tilapia. Both zebrafish and tilapia expressed a hepatocyte fatty acid desaturation/elongation pattern consistent with them being freshwater and planktonivorous fish. The data also showed that hepatic fatty acid desaturation/elongation was nutritionally regulated with the activities being higher in fish fed the VO diet compared to fish fed the FO diet. In zebrafish, the main effect of the VO diet was increased fatty acid Δ6 desaturase activity resulting in the production of significantly more 18:4n-3 compared to fish fed the FO diet. In tilapia, all activities in the pathway were greater in fish fed the VO diet resulting in increased amounts of all fatty acids in the pathway, but primarily eicosapentaenoic acid (EPA; 20:5n-3) and docosahexaenoic acid (DHA; 22:6n-3). However, the fatty acid compositional data indicated that despite increased activity, desaturation of 18:3n-3 was insufficient to maintain tissue proportions of EPA and DHA in fish fed the VO diet at the same level as in fish fed the FO diet. Practically, these results indicate that manipulation of tilapia diets in commercial culture in response to the declining global fish oil market would have important consequences for fish fatty acid composition and the health of consumers. Scientifically, zebrafish and tilapia, both the subject of active genome mapping projects, could be useful models for studies of lipid and fatty acid metabolism at a molecular biological and genetic level. This revised version was published online in August 2006 with corrections to the Cover Date.     

Production of diatom–bacteria biofilm isolated from Seriola lalandi cultures for aquaculture application

Controlled incorporation of selected microalgae and bacteria in aquaculture systems can be beneficial because they can act as microbiological control. That is why the characteristics of biofilm generated naturally in Seriola lalandi culture cages were analysed, their potential benefit to the growth of larvae was studied, and their controlled use for improving the larval viability and as a vector to improve incorporation of previously studied probiotic bacteria was tested. According to biodiversity results, these biofilms are composed of a diatom–bacteria mix showing a decrease in biodiversity in laboratory culture conditions being dominated by Navicula phyllepta and bacteria of the family Rhodobacteraceae. This can be produced on mesh substrates incorporated in bioreactors with rapid growth rate and adhesiveness. Preliminary results from the addition of substrates with this specific biofilm in larvae culture systems showed that it is consumed by the larvae without negative effects, while positive effects on the viability of larvae in combination with probiotics were observed. Considering preliminary results, the addition of these specific substrates with diatom–bacteria biofilms could be a good improvement for aquaculture systems and together with the use of probiotics can contribute to maintaining a stable and controlled system improving the viability of the larval fish culture in its early stages.