蓝点马鲛(Scomberomorus niphonius)大网目流刺网参数优化研究

在对黄渤海区蓝点马鲛(Scomberomorus niphonius)流刺网进行10种网型主要参数分析和筛选的基础上,2013年4月10日—5月15日,使用鲁昌渔64068渔船在黄海中南部对蓝点马鲛大网目流刺网网片做了4种规格网目尺寸的优化对比实验。结果显示,120 mm 网目尺寸的网片渔获尾数和重量最大,单位捕捞努力量渔获量(Catch per unit effort, CPUE)为0.1696尾/片·日和373.78 g/片·日,渔获效果优于110、115、125 mm 的网目。2015年4月12日—5月27日,使用鲁昌渔64068和鲁莱州渔66287渔船分别在黄海中南部、烟威外海、莱州湾海域对蓝点马鲛大网目流刺网主要参数做了网型优化对比实验。结果显示,鲁昌渔64068渔船使用120 mm 网目尺寸网型捕获的蓝点马鲛平均叉长为764.18 mm,最大个体叉长为1000 mm,最小个体叉长为490 mm;平均重量为3623.27 g,最大重量为7500 g,最小重量为850 g;CPUE 分别为0.1379尾/片·日和517.65 g/片·日。鲁莱州渔66287渔船使用122 mm网目尺寸网型捕获的蓝点马鲛平均叉长为780.47 mm,最大个体叉长为910 mm,最小个体叉长为600 mm;平均重量为4065.17 g,最大重量为7400 g,最小重量为2000 g;CPUE分别为0.0778尾/片·日和283.35 g/片·日。120 mm 网目尺寸网型的渔获效果优于122 mm 网目尺寸的网型,春季捕捞大个体蓝点马鲛的流刺网网目尺寸以120 mm 左右适合当前的蓝点马鲛群体组成。120 mm 网目优化网型主要参数为：渔具主尺度33.00 m×18.54 m,网衣规格500目×175目,网目尺寸为120 mm,网线材料为直径0.48 mm 的尼龙单丝(PAM),横向缩结系数为0.55,静浮力配备为267.9 g/m,浮沉比为1.768∶1。120 mm 网目优化网型的另一特点为通过缩小上缘网网目尺寸,用上缘网替代上缘纲和浮子纲2根纲索,既保持了渔具主要结构和性能不变,又节约了渔具材料。设计方法为：上缘网的网目尺寸=主网衣网目尺寸×主网衣横向缩结系数。     

蓝点马鲛(Scomberomorus niphonius)大网目流刺网参数优化研究

在对黄渤海区蓝点马鲛(Scomberomorus niphonius)流刺网进行10种网型主要参数分析和筛选的基础上,2013年4月10日—5月15日,使用鲁昌渔64068渔船在黄海中南部对蓝点马鲛大网目流刺网网片做了4种规格网目尺寸的优化对比实验。结果显示,120 mm网目尺寸的网片渔获尾数和重量最大,单位捕捞努力量渔获量(Catch per unit effort,CPUE)为0.1696尾/片·日和373.78 g/片·日,渔获效果优于110、115、125 mm的网目。2015年4月12日—5月27日,使用鲁昌渔64068和鲁莱州渔66287渔船分别在黄海中南部、烟威外海、莱州湾海域对蓝点马鲛大网目流刺网主要参数做了网型优化对比实验。结果显示,鲁昌渔64068渔船使用120 mm网目尺寸网型捕获的蓝点马鲛平均叉长为764.18 mm,最大个体叉长为1000 mm,最小个体叉长为490 mm;平均重量为3623.27 g,最大重量为7500 g,最小重量为850 g;CPUE分别为0.1379尾/片·日和517.65 g/片·日。鲁莱州渔66287渔船使用122 mm网目尺寸网型捕获的蓝点马鲛平均叉长为780.47 mm,最大个体叉长为910 mm,最小个体叉长为600 mm;平均重量为4065.17 g,最大重量为7400 g,最小重量为2000 g;CPUE分别为0.0778尾/片·日和283.35 g/片·日。120 mm网目尺寸网型的渔获效果优于122 mm网目尺寸的网型,春季捕捞大个体蓝点马鲛的流刺网网目尺寸以120 mm左右适合当前的蓝点马鲛群体组成。120 mm网目优化网型主要参数为:渔具主尺度33.00 m×18.54 m,网衣规格500目×175目,网目尺寸为120 mm,网线材料为直径0.48 mm的尼龙单丝(PAM),横向缩结系数为0.55,静浮力配备为267.9 g/m,浮沉比为1.768∶1。120 mm网目优化网型的另一特点为通过缩小上缘网网目尺寸,用上缘网替代上缘纲和浮子纲2根纲索,既保持了渔具主要结构和性能不变,又节约了渔具材料。设计方法为:上缘网的网目尺寸=主网衣网目尺寸×主网衣横向缩结系数。     

南海区康氏马鲛流刺网最小网目尺寸的初步研究

为促进南海马鲛刺网渔业的可持续发展和渔业资源的合理利用,于2012年和2015年分别进行了马鲛流刺网渔获组成和选择性试验。运用相对重要性指数(IRI)评价刺网的渔获组成,并在SELECT模型(share each length catch total)的架构下,利用正态分布(Normal)和双峰分布(Bi-normal)概率方程计算刺网对康氏马鲛(Scomberomorus commersoni)的选择性;进一步结合康氏马鲛的最小可捕规格,探讨南海区马鲛流刺网的最小网目尺寸。结果表明,康氏马鲛是南海马鲛流刺网最重要的渔获种类,其2012年和2015年IRI值分别为9 472.00和14 363.02;调查试验共捕获康氏马鲛295尾,其中2012年康氏马鲛的众数叉长组为751~800 mm(占32.52%),2015年康氏马鲛的2个众数叉长组分别为501~550 mm和701~750 mm;正态分布模型估算的选择性众数相对叉长为6.961;双峰分布估算的2个众数相对叉长值分别为4.440和6.728;根据模型残差值的大小,认为双峰分布的拟合效果较好;将康氏马鲛可捕规格定为380 mm的前提下,建议南海马鲛流刺网的最小网目尺寸可暂定为120 mm。     

东黄海区银鲳流刺网网目尺寸选择性研究

通过对90 mm、100 mm、110 mm和120 mm四种网目尺寸的银鲳流刺网进行选择性试验,利用Nomal、Lognomal、Gamma和Bi-nomal模型,进行银鲳渔获数据拟合,采用极大似然法估算模型参数。结果表明:(1)银鲳流刺网具有较好的捕捞对象和尺寸选择性;(2)银鲳流刺网网目尺寸选择性最适表达曲线为Gamma曲线;(3)分别利用选择性曲线和体周两种方法,推算最适捕获银鲳叉长与网目尺寸的线性关系,获得关系式为a=0.252 8L;(4)参照目前银鲳开捕叉长的最新研究结果,东黄海区银鲳流刺网最小网目尺寸标准建议调整为100 mm。     

南海北部马鲛流刺网渔获组成初步分析

为了明确南海北部马鲛（Scomberomorus）流刺网的渔获组成、主要种类的生物学特征、幼鱼比例等情况,2012年4月2113～24日在海南岛东北部渔场进行了调查研究,并对主要渔获种类的刺挂部位作了初步探讨。结果表明,马鲛流刺网的主要渔获种类为康氏马鲛（S．commersoni）、白b鲔（S．guttatus）和乌鲳（Formioniger）,其相对重要性指数（IRI）分别为10969．80、5502．49和1937．34;优势体长（叉长）组分别为701～800mm、501～600mm和231～250mm。建议尽快制定主要渔获种类的最佳开捕长度,进行渔具选择性试验,制定并出台最小网目尺寸等相关管理措施,以促进马鲛刺网渔业的可持续发展。     

黄渤海主要作业类型渔具的渔业资源利用

为了解黄渤海主要捕捞渔具对渔业资源的利用状况,2009年对张网、拖网和刺网等渔具类型的主要渔具种类开展了渔获结构和捕捞能力调查。结果表明,黄渤海的渔业生产以秋汛为主,张网、拖网和陷阱类渔具网目尺寸小,渔获选择I生差,大量捕捞小黄鱼0带鱼、蓝点马鲛和银鲳等传统经济鱼类当年发生的补充群体,幼鱼比例高。相比而言,刺网的网目尺寸大,渔获选择性强,渔获质量好,幼鱼比例低。建议将黄渤海渔具准入的最小网目尺寸暂定为5．5cm,待今后渔业资源结构发生变化和渔获上岸监管体系完善后再作分类调整。     

黄海双船变水层拖网网囊的网目选择性研究

为研究黄海双船变水层拖网网囊的网目选择性,2015年10月在黄海南部进行了网囊网目为40、54、60mm和70mm的套网拖网试验,采用Logistic选择模型和体周估算法对网囊网目选择性进行研究。结果表明,黄海双船变水层拖网渔获主要为蓝点马鲛、银鲳、带鱼、小黄鱼和鳀鱼;随着网囊网目尺寸的增大,逃逸率明显增加;极大似然法估算的模型参数,用AIC值检验拟合良好;估算出以变水层拖网捕捞达到可捕标准的部分经济鱼类相应的网囊网目尺寸,分别为小黄鱼71.0 mm、银鲳103.6mm、带鱼92.3mm、蓝圆鲹63.8mm。在黄海目前的渔业资源现状下,为保护小黄鱼、带鱼和鲐鲹等中小型经济鱼类的渔业资源,建议将双船变水层拖网的最小网囊网目尺寸定为60mm。     

黄渤海蓝点马鲛繁殖群体渔业生物学特征研究

利用2016年4-5月、2017年4月在黄、渤海调查采集的350尾蓝点马鲛(Scomberomorus niphonius)繁殖群体的生物学数据,对该繁殖群体的生物学特征进行了分析研究。结果表明:该繁殖群体的叉长范围为406～1010 mm,体重范围为533～7245 g,年龄组成为1～10龄,其中1龄与2龄个体分别占总数的39.2%与33.7%。采用Von Bertalanffy生长方程表示其生长特性,生长参数分别为L_∞=1246.9mm,K=0.11;雌雄性比为1:1.49,3龄前雄性个体数占优势,3龄后雌性个体数占优势。蓝点马鲛的产卵期在4～6月,产卵盛期为4～5月;其绝对繁殖力范围为9.2～127.5万粒/尾,平均50.5万粒/尾;绝对繁殖力随着年龄、叉长的上升逐渐增大,7龄后出现衰退现象。蓝点马鲛的优势饵料生物为玉筋鱼(Ammodytes personatus)、细鳌虾(Leptochela gracilis)。与历史记录比较,蓝点马鲛低龄化、小型化现象更加突出,摄食结构改变,繁殖期提前,繁殖力水平有减小的趋势。     

渤海口虾蛄三重刺网渔获组成及其捕捞性能分析

为研究渤海口虾蛄(Oratosquilla oratoria)三重刺网的捕捞性能,以河北省昌黎县的口虾蛄三重刺网为基础,于2013年4、5、7月在河北省秦皇岛近海进行50 mm、55 mm、60 mm和65 mm 4种网目尺寸的三重刺网作业性能比较实验,并与60 mm网目单片刺网和底拖网进行渔获对比分析。结果表明:三重刺网具有捕获渔场中绝大多数渔业种类的能力,选择能力较差。春季的渔获种类19种,为渤海近岸底层种类;夏季的渔获种类19种,除了小黄鱼(Pseudosciaena polyactis)和带鱼(Trichiurus lepturus)等底层鱼类外,还有日本鲭(Scomber japonicus)和蓝点马鲛(Scomberomorus niphonius)等中上层鱼类。春季的渔获结构单一,口虾蛄占渔获量的90%以上,主要兼捕种类鲜明鼓虾(Alpheus distinguendus)占4.1%,渔获选择性较好;夏季的渔获中,口虾蛄比例在75%左右,兼捕对象主要为小黄鱼、蓝点马鲛等经济鱼类的幼鱼,对渔业资源造成一定程度的损害,但渔获选择性比底拖网好。单片刺网的选择性最好,但渔获量低,只有三重刺网渔获量的30%左右,无法满足生产要求。建议开展渤海口虾蛄的可捕产量和分区域捕捞管理研究,开发作业性能更优越的口虾蛄专业捕捞渔具。     

青岛斋堂岛海域春季双桩竖杆张网网囊网目的选择性

2014年春季,采用平行作业法,以2a=16 mm(a为目脚长度)的菱形目网囊为对照网(CN),选取30 mm菱形目网囊和20 mm、30 mm、40 mm的方形目网囊(分别以30D、20S、30S和40S表示)作为实验网囊,对青岛斋堂岛附近海域的双桩竖杆张网进行了不同网目形状的网囊和不同网目尺寸方形目网囊的选择性比较试验。以多元统计分析方法进行种间选择性分析,以Logistic曲线作为选择性模型、采用极大似然估算法进行种内选择性分析。结果显示,与对照网相比,所有实验网囊均能有效释放玉筋鱼(Ammodytes personatus)、舒氏海龙(Syngnathus schlegeli)等低值渔获以及经济鱼类的幼体。比较不同网目形状网囊的渔获数据发现,方形目网囊较菱形目网囊具有更好的选择性,其中30S网囊对玉筋鱼和舒氏海龙的释放率(以尾数计)分别比30D网囊高4.61%和16.80%,对六丝钝尾虾虎鱼(Amblychaeturichthys hexanema)和日本枪乌贼(Loigo japonica)的50%选择体长(胴长)L50分别比30D网囊大38.47%和61.29%。比较不同网目尺寸方形目网囊的渔获数据发现,当实验网为20S和30S时,六丝钝尾虾虎鱼的L50分别为6.58 cm和7.20 cm,选择范围(SR)分别为3.02 cm和0.87 cm;当实验网为20S、30S和40S时,日本枪乌贼的L50分别为2.88 cm、4.03 cm和10.49 cm,SR分别为0.74 cm、0.52 cm和2.67 cm;不同尺寸的方形目网囊对这2种渔获物的L50随着网目尺寸的增大而增大。根据实验海区各种渔获物的经济价值、低值鱼类和幼鱼的释放比例以及分拣渔获物的劳动力投入等综合分析认为,30 mm方形目网囊在保护渔业资源的同时对渔业生产不会造成显著影响,建议予以推广。     

Susceptibility by amberjack (Seriola dumerili), yellowtail (S. quinqueradiata) and Japanese flounder (Paralichthys olivaceus) to Neobenedenia girellae (Monogenea) infection and their acquired protection

This study investigated: 1) susceptibility differences to infection by Neobenedenia girellae (Capsalidae) between amberjack Seriola dumerili (Carangidae), yellowtail S. quinqueradiata and Japanese flounder Paralichthys olivaceus (Paralichthyidae); 2) growth and egg production of N. girellae on each fish species; 3) acquired protection of each fish species against this parasite. The number of N. girellae on S. dumerili was significantly higher than on S. quinqueradiata and P. olivaceus when these fishes were exposed to oncomiracidia in the same aquarium. Neobenedenia girellae growth on S. dumerili was fastest and, thus the number of eggs laid by parasites on S. dumerili was greater than on the other two species. Seriola dumerili and P. olivaceus, which were previously infected with N. girellae and treated by freshwater bath, acquired partial protection against re-infection by N. girellae. The relative re-infection of three S. dumerili individuals out of eleven individuals was markedly low compared with the initial infection, and the relative initial infection and re-infection on two P. olivaceus out of eleven individuals was markedly low. The results of this study could be useful to control N. girellae infections when cultivating S. dumerili, S. quinqueradiata and P. olivaceus.     

AFLP-based genetic linkage map of marine shrimp Penaeus (Fenneropenaeus) chinensis

This paper presents the genetic linkage map of the Chinese shrimp Penaeus (Fenneropenaeus) chinensis constructed with 472 AFLP markers. A hundred F₁ progeny from an intercross between a female from the new variety “Yellow Sea No. 1” and wild caught male used for the mapping study. Two separate maps were constructed for each parent. The female linkage map consisted of 197 marker loci forming 35 linkage groups and spanned a total length of 2191.1 cM, with an average marker space of 13.5 cM. The male map consisted of 194 marker loci mapped to 36 linkage groups and spanned a total length of 1737.3 cM, with an average marker spacing of 11.0 cM. The level of segregation distortion observed in this study was 12.2%. The estimated genome length of P. chinensis was 3150.3 cM for the female and 2549.3 cM for the male, respectively. The observed genome coverage was 69.6% for the female and 68.1% for the male map. The linkage maps constructed in this study provide basic information for further linkage studies on Chinese shrimp, and more importantly, the construction of the maps are part of the work of the genetic breeding programs which will be used for growth discovered in the QTL analysis of P. chinensis.     

太平洋鳕RAG1和IgM基因荧光定量PCR方法的建立及初步应用

为研究太平洋鳕发育早期特异免疫系统形成的机制,通过RAG1和IgM基因的转录水平衡量特异免疫系统的发育特点.根据GenBank中RAG1和IgM的序列信息,分别设计1对特异引物,从太平洋鳕头肾中扩增得到RAG1和IgM的基因片段.将所获基因片段分别插入到克隆载体pMD18-T中,从而构建太平洋鳕RAG1和IgM基因的质粒标准品.建立并优化太平洋鳕RAG1和IgM基因绝对荧光定量PCR方法.为进一步验证该方法的可靠性,分别利用绝对定量和相对定量检验目的基因在太平洋鳕早期发育过程不同组织内的表达差异.以优化后的绝对荧光定量PCR方法检测不同发育时期太平洋鳕RAG1和IgM的表达情况.结果显示,RAG1的回归方程为y=-3.266x+33.77,回归系数R2=0.996;IgM的回归方程为y=-3.119x +27.61,回归系数R2 =0.998.绝对定量和相对定量结果在基因转录趋势上显现出一致性,即RAG1基因在胸腺和头肾中表达,且在胸腺中的表达量显著高于头肾中的表达量,在肝脏和脾脏中无表达;IgM基因在胸腺、头肾、肝脏和脾脏中均有表达,其中脾脏中表达量最高,其次是头肾.RAG1基因在太平洋鳕发育早期的表达水平很低,到61日龄(days posthatching,dph)至95 dph表达量显著提高;IgM基因在早期表达水平同样很低,到33 dph至61 dph才有明显表达,在95 dph时表达量显著提高.研究表明,本实验方法可靠,特异性较强,可成功对目标基因转录水平进行检测.     

Composition in essential and non-essential elements of early stages of cephalopods and dietary effects on the elemental profiles of Octopus vulgaris paralarvae

During the present study, we aimed at providing a first look at the elemental composition of the early stages of cephalopods as an approach to their elemental requirements in culture. Essential and non-essential elemental profiles of the European cuttlefish Sepia officinalis, the European squid Loligo vulgaris and the common octopus Octopus vulgaris laboratory hatchlings and wild juveniles were analysed. In addition, for O. vulgaris we determined elemental profiles of mature ovary, eggs in different stages of development and followed possible effects of four dietary treatments during paralarval rearing, also analyzing elemental content of the live preys Artemia nauplii and Maja brachydactyla hatchling zoeae. Content was determined for essential (As, Ca, Cr, Co, Cu, Fe, K, Mg, Mn, Na, Ni, P, Rb, S, Sr, Zn) and non-essential (Ag, Al, Ba, Cd, Hg, Pb) elements. The content in non-essential elements found in hatchlings and juveniles of the three species analyzed here seems to be far lower in comparison with subadult and adult stages of coastal cephalopods. In the octopus eggs, the non-essential element concentrations remained globally low compared to hatchlings and juveniles indicating the absorption of these elements along the ontogenetic development. The elemental composition of the octopus ovary and of the eggs, hatchlings and juveniles of the three cephalopod species analyzed here showed a high content in S. As expected, the calcified internal shell of the cuttlefish, rich in Ca and Sr, originates the main difference between species. It is remarkable the richness in Cu of hatchling octopus, that may indicate a particular nutritional requirement for this element during the planktonic life. The reared octopus paralarvae feed on Artemia nauplii, a prey with relatively low Cu content, showed nearly half Cu content that the “natural” profile of octopus hatchlings or wild juveniles. This suggests a dietary effect and/or an indication of the poor physiological stage of the Artemia-fed paralarvae. At the present, the percentage of essential element absorption by food or seawater is unknown for cephalopods and should be determined in the future to understand their feeding requirements in culture.     

Recruitment and effects of Discocotyle sagittata (Monogenea) infection on farmed trout

Farmed rainbow trout Oncorhynchus mykiss and brown trout Salmo trutta were monitored over 3 years for infection with the blood-feeding gill fluke Discocotyle sagittata. Parasite transmission is seasonal: new infections take place during summer/autumn, and transmission is generally negligible during winter/spring. There are 2 sources of infection for naïve fish-of-the-year: limited invasion when fish are in the raceways by riverborne larvae originating external to the farm; and internally, within the farm, when 0+ fish are transferred to ponds previously occupied by older cohorts of infected fish. Thereafter, infection levels continue to increase in rainbow trout primarily through transmission within the farm. Prevalence rose to 100% in 1+ fish by the end of their second summer. In O. mykiss, mean abundance reached 194 worms/host for 1+ fish (up to 489 worms/host) and 160 worms/host for 2+ fish. By contrast, in S. trutta, parasite prevalence never exceeded 85% and, after the first year's invasions, infection levels decreased over time: in 1+ and 2+ brown trout, parasite mean abundance was < 4 (maximum 15) worms/host. We present evidence of the detrimental effects of D. sagittata on the host: high burdens are associated with pale gills, decreased body condition and host mortality. Parasite burdens become overdispersed during the warmer part of the year, as prevalence and mean abundance increase. However, the degree of parasite overdispersion decreases over winter; we cannot distinguish whether decreased aggregation is due to parasite losses from infected fish (including immune-mediated parasite mortality) or parasite-induced host mortality.     

吉富罗非鱼与奥利亚罗非鱼自繁与杂交 F1遗传特性与抗病力分析

以吉富罗非鱼与奥利亚罗非鱼为繁育亲本,采用完全双列杂交繁育4组F1,将初始规格基本一致的4组罗非鱼饲养100 d后,运用“加性-显性”遗传分析模型,分析了4组F1罗非鱼8个生长相关性状杂种优势、遗传效应以及与性状间的相关性.结果表明:(1)F1群体平均优势为0.129 4～0.368 4.除尾柄长超亲优势较大外,其他性状的群体超亲优势较小或表现出负向超亲优势.(2)8个性状的广义遗传率(HB)为0.714 2～0.995 3,表明加性效应和显性效应对性状的遗传变异影响极显著(P＜0.01).除尾柄长外,其他性状的狭义遗传率(HN)介于0.469 4～0.737 9,表明加性遗传方差在表型方差中所占比率较高.(3)体质量、全长、体长、体高、体宽、头长、尾柄长、尾柄高性状之间表型相关在0.776 6～0.999 7范围内,而遗传相关在0.994 1～1.000 0之间,表明这些性状间都存在极显著的正相关.取样结束后,采用3.95×106 CFU/mL的海豚链球菌菌液进行腹腔感染,吉富罗非鱼自繁组F1代12 h后出现死亡,而奥利亚罗非鱼自繁组F1192 h后才出现死亡.384 h后,吉富罗非鱼自繁组F1死亡率为40％,正反交组F1分别为20％和23.3％,奥利亚罗非鱼自繁组F1死亡率最低,为6.67％.研究结果表明,除尾柄长外,杂交F1的其他性状不具备超亲优势,然而杂交可以提高选育后代的抗病力.     

4种岩礁性鱼类视网膜感光细胞和最小分辨角的比较

运用组织学方法,对铠平鲉、花斑平鲉、大泷六线鱼和斑头六线鱼的性成熟个体视网膜光感受细胞和最小分辨角进行了比较研究。结果表明:(1)大泷六线鱼和斑头六线鱼视锥细胞的排列方式为4个双锥细胞围绕一个单锥细胞构成正方形或者菱形镶嵌结构,铠平鲉和花斑平鲉的双锥细胞排列成行,单锥细胞随机分布于4个双锥细胞之间;分析认为,大泷六线鱼和斑头六线鱼视锥排列方式更复杂,单锥细胞与双锥细胞的比值(SC/DC)更高,推测其具有更好的色觉。(2)铠平鲉和花斑平鲉视柱细胞与视锥细胞的比值(R/C)大于7∶1,而大泷六线鱼和斑头六线鱼的R/C小于3∶1;推测铠平鲉和花斑平鲉更适合在夜间活动,而大泷六线鱼和斑头六线鱼属白天活动的鱼类。(3)铠平鲉、花斑平鲉、大泷六线鱼和斑头六线鱼视锥细胞密度最高处分别位于视网膜的腹-颞(V-T)区、腹区(V)、腹-颞(V-T)区和背-颞区(D-T),与各自的生活习性和摄食行为相适应。(4)4种岩礁性鱼类的成熟个体中,铠平鲉的最小分辨角最大,其次为花斑平鲉和大泷六线鱼,斑头六线鱼的最小分辨角最小。分析认为,即使是同样栖息于礁区的鱼类,其视觉特征也存在差异,这或许可以为岩礁区选择性渔具的开发提供一些有益的参考。     

罗非鱼海豚链球菌病的病理学观察

采用光学显微镜和透射电子显微镜技术分别对患海豚链球菌病的罗非鱼的病理学变化进行了研究。分离的病原菌革兰氏染色呈阳性,透射电镜负染观察菌体球形或卵圆形,直径0.6~1.0μm,多数呈链状排列。组织学病变主要表现为全身多组织、器官水肿,出血、变性、坏死以及炎症反应,特别是肝、脾、肾和脑分别表现为肝炎,脾炎,间质性肾炎和脑膜炎。超微结构观察发现病鱼肝、脾、肾、脑、心肌和骨骼肌等器官的细胞超微结构都有较为严重的破坏,细胞核畸形,染色质浓缩或边集,粗面内质网囊泡化及脱粒,线粒体肿胀,嵴断裂或溶解消失。研究表明,海豚链球菌能造成罗非鱼全身性组织器官病变,致使器官功能障碍,正常生理代谢调节紊乱,最后导致死亡。     

半滑舌鳎StAR基因克隆及其在不同组织的时空表达分析

利用半滑舌鳎性腺转录组测序获得的StAR基因部分序列,设计RACE引物,克隆了半滑舌鳎StAR基因的cDNA序列,全长为1 294 bp,5'端UTR为132 bp,3'端UTR为310 bp,开放阅读框(ORF)为852 bp,共编码283个氨基酸。将半滑舌鳎StAR基因与其他物种StAR基因进行氨基酸同源性分析,结果显示,半滑舌鳎StAR与塞内加尔鳎、大口黑鲈、花鲈、金头鲷的同源性都达到了85%,与虹鳟、斜带石斑鱼及日本鳗鲡的同源性分别为81%、83%和76%。雌、雄鱼不同组织StAR基因的表达分析表明,StAR基因在雄鱼性腺中高表达,在雄鱼的肝脏、脑及心脏中表达量较低,而在雄鱼的其他组织中不表达;在雌鱼肠中不表达,在其他组织(卵巢、肝脏、脾脏、脑、垂体、肌肉、心脏、肾脏)中微量表达。荧光定量PCR分析不同组织与不同时期性腺表达谱表明,雄鱼性腺中StAR基因的表达量显著高于雌、雄鱼其他各组织(P0.05),提示StAR基因对雄鱼精巢发育起重要作用。雄鱼不同时期表达谱分析结果显示,StAR基因在66天前的精巢中不表达,在150天时表达量急剧增加,至2龄时表达量最高,3龄时表达量下降,说明该基因在精巢发育成熟过程中起重要作用。原位杂交结果显示,StAR基因主要在雄鱼精巢的精子细胞中表达,而在雌鱼的卵巢中不表达。研究表明,StAR基因在半滑舌鳎精巢发育中发挥作用,且可能在精子形成中发挥重要作用。     

The mariculture potential of the Indonesian reef-dwelling sponge Callyspongia (Euplacella) biru: Growth, survival and bioactive compounds

In the present study, the Indonesian reef-dwelling sponge Callyspongia (Euplacella) biru de Voogd [de Voogd, N.J., 2004. Callyspongia (Euplacella) biru spec. nov. (Porifera: Demospongiae: Haplosclerida) from Indonesia. Zool. Meded. 78, 477-483.] (Demospongiae, Callyspongiidae) was cultured for a period of 6 months at three sites in the Spermonde Archipelago, Indonesia. A total of 250 sponge cuttings of two size classes (30 and 40 mm in length) were threaded on either polyethylene or very fine nylon rope and attached to horizontal mooring systems. All sponge cuttings were photographed at regular time intervals. Survival rates were high (82%-100%) among all treatments used. Growth rates differed significantly among treatments, but did not differ significantly per location, farming method, explant size and explant position obtained from the parent sponge. The concentration of the bioactive compound amphitoxin in the cultured explants was not significantly lower than in the natural population, but the concentration did vary significantly between explants used in the different treatments, and also between the different fragments of the natural population. The high growth potential and high survival rate suggest that this sponge species is a promising candidate for further mariculture development.