Opaque2基因对糯玉米子粒品质的影响分析

玉米Opaque2(O2)基因突变显著影响子粒胚乳中蛋白体形成、淀粉含量、氨基酸组成等,其中,赖氨酸含量提高使营养品质大幅度提升。通过回交转育的方法构建17份糯玉米o2近等基因系,对子粒表型、种皮厚度、百粒重和粗淀粉含量等性状分析。结果表明,与对照相比,16个近等基因系子粒明显皱缩、胚乳变为粉质,6个近等基因系种皮厚度增加,11个近等基因系百粒重显著降低,12个近等基因系子粒粗淀粉含量显著降低。由此表明,o2基因的导入对糯玉米子粒表型、产量、淀粉含量等主要起负调控作用,影响程度与导入背景密切相关。     

寒地半干旱区鲜食玉米品种适应性和品质性状分析

以18个鲜食玉米品种为试验材料,针对寒地西部半干旱地区的气候条件,研究不同类型鲜食玉米子粒鲜食期的营养成分的积累动态及品质变化。结果表明,鲜食玉米灌浆期子粒的可溶性糖、粗淀粉、蛋白质、子粒含水率和维C含量在不同灌浆期、不同品种间差异极显著。随着子粒的发育,不同类型鲜食玉米品种的可溶性总糖、可溶性蛋白含量和子粒含水率呈下降趋势,粗淀粉含量呈上升趋势,维C含量呈先上升后下降的动态曲线变化。根据鲜食玉米营养成分与品质的关系,通过子粒含水率结合蛋白质含量和粗淀粉含量确定糯玉米的最佳采收期,子粒含水率结合可溶性糖含量和维C含量确定甜玉米的最佳采收期。     

玉米自交系齐319空间诱变系的生物学效应分析

以两份玉米子粒类型空间诱变系SP-M1、SP-M2以及野生型玉米自交系齐319为试验材料,对田间农艺性状、穗部性状、子粒的营养品质性状和胚乳内部显微结构进行分析。结果表明,与野生型相比,两个诱变系在株高、穗位高、粒长、百粒重等田间农艺性状和穗部性状上发生显著或极显著变异,且变异方向不同,SP-M1和SP-M2赖氨酸含量显著高于野生型,SP-M2淀粉含量显著高于野生型,蛋白质含量显著低于野生型。2个诱变系与齐319在胚乳边缘组织、胚乳内部组织、淀粉粒、基质蛋白等结构存在差异,淀粉粒的组成比例和排列顺序是影响胚乳超微结构差异的主要原因。     

不同鲜食玉米灌浆期子粒主要营养成分的积累动态

通过理化方法对5种不同类型的14个鲜食玉米品种在不同灌浆期子粒的营养成分进行分析,研究不同类型鲜食玉米子粒主要营养成分的积累动态,筛选能够指示不同类型鲜食玉米最佳采收期的客观理化指标。结果表明,鲜食玉米灌浆期子粒的可溶性总糖、蔗糖、还原糖、蛋白质、支链淀粉、直链淀粉和含水量在不同品种间、不同灌浆期间差异极显著。随着子粒的发育,不同类型鲜食玉米品种的可溶性总糖和蔗糖含量呈动态曲线变化,变化趋势都是先上升后下降;还原糖含量和含水量呈不断下降的趋势;直链淀粉和支链淀粉含量呈不断上升的趋势;蛋白质含量变化幅度很小,变化趋势不明显。根据鲜食玉米营养成分与品质的关系,提出子粒含水量结合可溶性糖含量和粗淀粉含量可分别作为甜玉米和糯玉米最佳采收期的指标。     

种植密度对甜玉米与鲜食糯玉米产量与品质性状的影响

采用田间试验的方法,研究低密度(3.5万株/hm2,LD)、中密度(5.5万株/hm2,MD)、高密度(6.5万株/hm2,HD)种植对甜玉米(520)、糯玉米(吉农糯112)商品品质、食用品质以及部分营养品质的影响。结果表明,种植密度对鲜食玉米商品品质的影响较大,高密度种植会显著降低有效穗长、出籽率以及百粒重等商品性状。甜玉米皮渣率以及糯玉米的种皮厚度受种植密度影响较大,高密度处理下甜玉米(520)的皮渣率比低密度处理增加29.2%,糯玉米(吉农糯112)种皮厚度下降24.78%。种植密度对脂肪、可溶性总糖以及赖氨酸含量的影响不显著,显著影响鲜食玉米子粒中蛋白的含量,高密度处理下甜玉米(520)与糯玉米(吉农糯112)蛋白含量比低密度处理分别降低近1个百分点。     

鲜食糯玉米品质差异及适宜采收指标的研究

以8个鲜食糯玉米品种为试验材料,研究授粉后17～29 d不同采收期百粒鲜重、子粒粗淀粉含量、含水率与鲜穗蒸煮品评分的变化及关系。结果表明：4项均值在不同品种间及不同采收期间差异极显著;最佳品质期在授粉后的时期及长短、最佳品质时的百粒鲜重占最大粒重的比例、子粒粗淀粉含量在品种间均存在差异;推迟采收有利于增加百粒鲜重;子粒含水率在59%～64%时可作为鲜穗最适采收期。     

玉米甜、糯性状育种的遗传学基础

甜玉米和糯玉米是一类淀粉合成途径不同、基因发生突变导致子粒中含有高可溶性糖或高支链淀粉的玉米,是可直接食用的鲜食玉米类型。介绍玉米甜、糯性状形成的生物化学基础及其遗传特征,详细阐述单果穗甜糯形成的遗传学基础与机制,利用基因编辑技术进行隐性性状高效遗传改良具有无需表型选择并克服连锁累赘的优势。     

两个玉米品种胚乳发育的比较

以农乐988和扬糯1号两个品种的玉米颖果为材料,利用树脂半薄切片、组织化学染色及生理测定等方法研究胚乳组织和细胞的发育过程。结果表明,两个玉米品种粒重及淀粉含量的变化呈S形生长曲线;可溶性糖变化呈单峰曲线,授粉后12 d含量最高;总蛋白含量在授粉后6 d较高。颖果粒重和总蛋白含量农乐988>扬糯1号;可溶性糖及淀粉含量扬糯1号>农乐988。授粉后0~2 d胚乳处在游离核期,授粉后3~5 d胚乳处在细胞化期,授粉6 d以后胚乳细胞开始分化,内胚乳细胞出现淀粉体和蛋白体。胚乳中淀粉的积累由颖果顶端向基部、由胚乳外层向中央推进。扬糯1号胚乳发育较提前,失活较晚;农乐988胚乳发育相对滞后,失活较早。玉米胚乳发育和颖果发育关系紧密,胚乳游离核期和细胞化期相当于颖果形成期,胚乳分化期相当于颖果乳熟期,胚乳成熟期相当于颖果蜡熟期与完熟期。     

收获后贮藏时间对鲜食玉米品质的影响

以12个糯玉米品种和6个甜玉米品种为试验材料，研究采收后室温下（28℃）放置0、8、16、24、32 h后对子粒中糖含量、淀粉含量、蛋白含量蔗糖代谢酶活性等的影响，探讨鲜食玉米采摘后贮藏时间对鲜食品质的影响。结果表明，鲜食玉米随着采收后时间的延长，子粒可溶性糖含量、蔗糖含量、果糖含量、还原糖含量、淀粉含量、可溶性蛋白含量以及蔗糖代谢酶活性均呈下降趋势，常温下糯玉米品质变化大于甜玉米。因此，为保证鲜食玉米的子粒品质，生产上糯玉米采收后8 h内进行冷冻贮藏。甜玉米中，先正达OVERLAND于采收后16 h内进行冷冻贮藏，京科甜183、和甜一号、和甜糯一号、先正达米哥、郑甜66于采收后8 h内进行冷冻贮藏。     

玉米子粒性状和子粒赖氨酸含量的相关及通径分析

以28份玉米自交系为材料,对9个子粒性状与子粒赖氨酸含量进行了相关和通径分析。结果表明,油分、胚重、胚比(胚重与胚乳重的比值)、胚乳硬度等级与赖氨酸含量呈显著或极显著正相关;淀粉含量、密度(粒重与体积之比)、胚乳重与赖氨酸含量呈显著或极显著负相关;百粒重和粗蛋白含量与赖氨酸含量相关不显著。各子粒性状对赖氨酸含量的直接影响力大小依次为胚比>胚重>胚乳重>胚乳硬度等级>密度>油分>淀粉含量>粗蛋白含量>百粒重。其中,胚比对子粒赖氨酸含量起关键作用。     

玉米opaque2突变体子粒表型及营养成分分析

通过回交转育构建o2突变型的近等基因系CAL58/o2,对其子粒进行扫描电镜和透射电镜观察,发现与CAL58相比,CAL58/o2中淀粉颗粒排列松散,形状更加规则,CAL58/o2子粒中蛋白体体积变小。分析子粒的营养成分,CAL58/o2赖氨酸含量极显著提高了55.88%,氨基酸总量显著减少9.79%,粗蛋白显著减少8.86%,粗脂肪显著增加30.96%,粗淀粉显著增加5.87%。同时,提取成熟子粒醇溶蛋白进行比较,CAL58/o2子粒的α、β-醇溶蛋白含量明显降低,γ-醇溶蛋白含量增加。     

Effects of the replacement of Glu-A1 by Glu-D1 locus on agronomic performance and bread-making quality of the hexaploid wheat cv. Courtot

The aim of this study was to evaluate the cumulative and interactive effects on wheat (Triticum aestivum L.) gluten strength and mixing properties of dough associated with the duplication of the Glu-D1 locus. A partially isohomoeoallelic line RR240, in which a segment of the wheat chromosome 1D containing the Glu-D1 locus encoding the Dx2 + Dy12 subunits and translocated to the long arm of the chromosome 1A through homoeologous recombination, was assessed. Agronomic traits and yield components were studied in the translocated line RR240 and compared with the control line cv. Courtot. Both lines were evaluated under field conditions in two experimental years. Technological effects resulting from the duplication of HMW glutenin subunits Dx2 and Dy12 were evaluated using the Alveograph test, the Mixograph test and the baking test. The RR240 line was shown to have a lower agronomic performance for 1000-kernel weight and grain yield. However the duplication of the Glu-D1 allele was associated with a significant effect on dough strength and mixing resistance, and on the Zeleny sedimentation volume. Baking parameters were not significantly modified between both lines although the score values of the CNERNA test were observed to be slightly higher in RR240 than in Courtot.     

The effect of a seaweed concentrate on the in vitro growth and acclimatization of potato plantlets

Summary The seaweed concentrate ‘Kelpak’ is used commercially in the greenhouse and field to improve plant quality. ‘Kelpak’ was added to the in vitro culture medium of potato cv. ‘BP1’ and also applied as a leaf/soil drench immediately after transplanting. The addition of 0.25% seaweed concentrate to the medium improved plantlet quality and led to better establishment in the greenhouse. No beneficial effect of seaweed concentrate in the tissue culture medium was observed if a second cutting was part of the micropropagation process. Additional application of 0.5% seaweed concentrate in the greenhouse to plantlet cuttings derived from tissue culture was not beneficial.     

Studies on jicama juice processing

Juice was extracted from jicama (Pachyrrizus erosus Urban) and clarified using a 10 000 daltons molecular weight cut-off membrane to improve its stability. Ultrafiltered juice was tested for general composition and Hunter color. Ultrafiltration (UF) retentate and UF permeate showed some changes, compared to fresh juice, in total and soluble solids, total sugars, and nitrogen, whereas ash and pH remained constant. Hunter color of juice samples exhibited some variation by UF. Results suggest that UF has potential to produce jicama juice with desirable and stable aroma and flavor.     

Estimating tuber dry matter concentration from accumulated thermal time and soil moisture

Summary The tuber dry matter concentration, [DM], of crops of potato (Solanum tuberosum L.) was related using regression analysis, to time, thermal time, incident radiation accumulated from plant emergence, and soil moisture deficit (SMD). Variation in [DM] was best accounted for by the regression model that was a function of thermal time above a base of 0 °C accumulated from plant emergence, and SMD. When validated against an independent data set, there was good agreement between observed and estimated [DM] with a linear relation accounting for 79.3% of the variance.     

Seed protein yield from someCrotalaria spp andin vitro nutritional quality of that fromC. juncea

Seed Protein Concentrates (SPC) of 6 species ofCrotalaria were extracted and the extractabilities of SPC, total N and protein N determined. SPC of high yielding species (C. juncea) was analysed for the contents of amino acids, ash, phosphorus, sugar, starch, fibre and calories, as well as forin vitro digestibility. Results indicate the promising potential ofC. juncea for SPC yield.     

Texture,processing and organoleptic properties of chickpea-fortified spaghetti with insights to the underlying mechanisms of traditional durum pasta quality

Nutritionally enhanced spaghetti was prepared from durum semolina fortified with 0–30% desi chickpea ‘besan’ flour. This study examined the dough rheology, processing ease and quality attributes of the fortified spaghetti including protein, starch, texture (firmness, resilience and stickiness), colour, cooking loss, and organoleptic acceptability. Chickpea-fortified spaghetti was acceptable to consumers, had reasonable pasta quality, including lower cooking loss and less stickiness than the control spaghetti and retained firmness better than durum after refrigeration. This study suggests that chickpea-fortified spaghetti may be suited to uses such as fresh pasta, in soups, canning, and microwave re-heating. In addition, this study has added to the understanding of the underlying mechanisms of pasta quality. The main findings were: (1) gluten content/composition appears to be more important than protein content for pasta firmness; (2) the protein–polysaccharide matrix appears to be more important than the starch composition for cooking loss; (3) increased protein and amylose contents were associated with decreased pasta stickiness; (4) cooking loss and stickiness were not necessarily as strongly related as commonly believed. Further research into these theories is necessary to fully understand the underlying mechanisms of pasta quality.     

籼稻杂种一代品质性状与其亲本关系的研究

以5个籼稻优质品种(七桂早25、新丝苗1号、新锦占1号、早选2、早优占30)和4个籼稻非优质品种(光明、金占、特青、三二矮,其中特青、三二矮为高产品种)为材料配制杂交组合进行品质性状的研究。结果表明,以硬胶稠度作亲本的组合,其杂种一代都为硬胶稠度。F1植株籽粒品质性状的表达较复杂,如亲本为高直链淀粉含量的不同组合,可以出现高、中、低含量的杂种一代,因此不能单独根据某一性状来确定亲本的选配。粒形和脂肪含量可以作为衡量米质等级的指标。选用优/优、优/非优组合,可以育出长粒形、高脂肪含量的杂种一代。选用优/优或优/非优组合,更可能获得既优质又高产的杂种一代。七桂早/早选2是优质的最佳组合。七桂早/新丝苗、七桂早/金占、七桂早/早优占是优质高产的最佳组合。     

Development of rice introgression lines with brown planthopper resistance and KDML105 grain quality characteristics through marker-assisted selection

‘Khao Dawk Mali 105’ (KDML105), a Thai aromatic rice cultivar, has been accepted in markets as a prime jasmine rice with premium prices. It has been extensively used as a parental line to develop new cultivars for rainfed lowland areas in Thailand because of its favorable quality and fragrance. However, this cultivar is highly susceptible to brown planthopper (BPH), a phloem sap-feeding insect pest of rice. The main objective of this study was to combine KDML105 essential grain quality traits with BPH resistance from the donor cultivar, ‘Rathu Heenati’. The linkage drag between Bph3 and Wx^a alleles was successfully broken by phenotypic and marker-assisted selections. All introgression lines (ILs) developed in this study showed a broad spectrum resistance against BPH populations in Thailand and had KDML105 grain quality standards. Finally this study was revealed that the ILs can be directly developed into BPH resistant varieties or can be used as genetic resources of BPH resistance to improve rice varieties with the Wx^b allele in rice breeding programs.