Protein quality and digestibility of new high-lysine barley varieties in growing rats

Four new high-lysine barley mutants, the variety Lysimax, with the high-lysine genelys3a and the mutants mother variety Sultan were grown in a field trial in 1993 at Risø, Denmark. Mutants 609, 1242, 1385 and 1405 yielded in the range of 89 to 98 percent and cv Lysimax yielded 102 percent of cv Sultan (100 percent). One-thousand kernel weights for the mutants were in the range of 87 to 97 percent and cv Lysimax 83 percent of cv Sultan (100 percent). Protein contents of the mutants were slightly higher, in the range of 13.2 to 13.6 percent, than of cv Sultan (13.1 percent) and Lysimax which had a protein content of 12.6 percent. Fat content was higher in Lysimax and in the mutants except for mutant 1385 than in cv Sultan while dietary fibre contents of the barleys were similar. The levels of -glucans and starch were usually lower in Lysimax and in the mutants. The highest lysine levels: 4.6, 4.0 and 3.7 g/16 g N occurred in cv Lysimax and mutants 609 and 1405 compared to 3.3, 3.3 and 3.2 for cv Sultan and mutants 1242 and 1385, respectively. Mutants 609 and 1405 and cv Lysimax also had higher levels of threonine, histidine and valine. The increased lysine contents resulted in large, at most 20 percent, increases in biological value; 88.8, 81.7 and 78.3 percent for cv Lysimax and mutants 609 and 1405 compared to 74.2 percent for cv Sultan. True protein digestibilities and energy digestibilities were slightly lower in Lysimax than in Sultan, 5.3 and 4.3 percentage units, respectively. It is concluded that the development of high-lysine barley varieties is very beneficial for meeting the requirements of indispensable amino acids for humans and monogastric animals. In addition, nitrogen excretion into the environment is drastically reduced due to the higher biological values of the mutants.     

Effect of alkaline treatment (‘akanwu’) and supplementary value of corn or crayfish on the protein quality of breadfruit (Treculia Africana)

The effect of treatment and supplementary value of corn (C) or crayfish (CR) on the protein quality of breadfruit (Treculia Africana) flours were studied in eighteen young rats. The 7030 or 701515 (Protein basis) combinations of breadfruit flours and corn or crayfish or both provided 1.6g N/100 g diet for the 35 day study. The addition of akanwu to the cooking water reduced cooking time and crude protein and saved fuel. The addition of akanwu and replacement of CR with C was not beneficial as judged by the parameters tested except for the wt. gain and PER. On the other hand, when crayfish was the only source of supplementary protein (30%) to breadfruit cooked without akanwu there were increases in all parameters tested over those with added akanwu except for the N intake, wt. gain, and PER.These results appear to suggest that addition of akanwu to TA was detrimental to its protein utilization and that TA appears to be an economic source of N in areas where it is a staple. Based on the results of this study, one would suggest that the use of akanwu as a tenderizer should be seriously looked into before further use.     

Verbreitung des Wissens über die ‘innere’ Qualität von Gemüse und Obst in der Öffentlichkeit

Zusammenfassung Anhand praktischer Beispiele werden qualitätsrelevante Problemkreise vorgestellt und eingehend diskutiert, so Erzeugerberatung — Weg der Ware vom Erzeuger zum Verbraucher — Äußere Beschaffenheit — Innerer Wert — Präsentation der Verkaufsware — Erhaltung der Frische — Berücksichtigung ernährungsphysiologischer Kriterien. Es wird ein Publikationsmodell — bereits 1950 entwickelt — vorgeschlagen, das weite Kreise der Züchter, der Erzeuger, der Fabrikanten, des Handels und der interessierten Verbraucherschaft sowohl ansprechen als auch das Verständnis der einzelnen Gruppen untereinander wecken könnte.Denn letztlich sollte es doch im Bermühen des Erzeugers, des Transporteurs, des Handels, des Verarbeiters von Obst und Gemüse in erster Linie darum gehen, das Wohlergehen, die Gesunderhaltung des Verbrauchers im Auge zu haben. Das wäre jedenfalls die Vorstellung und Erwartung der ernährungsphysiologisch und ernährungs-hygienisch ausgerichteten Qualitätsforscher.

---

Public extension about the inner Quality of Vegetables and Fruit

As usually practized in vegetable and fruit cultivation problems relevant to the inner quality are presented and discussed, e.g. Information of consumers, Shipping products from producer to consumer, External Quality — inner Quality — Offering of products — Conserving of freshness — Regarding the nutritional value.In order to evaluate these problems a close co-operation of breeders, producers, manufacturers, dealers and the interested consumers seem to be necessary. This is possible through a model and system of information, similar to the so-called AZA which were first published in 1950.

---

---

Referat, gehalten auf der Sitzung der neukonstituierten Kommission Öffentlichkeitsarbeit der DGQ am 1.10.1976 in Geisenheim/Rhein.     

Microbiological evaluation of tofu and tempeh during processing and storage

Tofu samples consisting of Fresh Tofu, Herb Tofu, Tofu Paste, Fried Tofu, Tofu Burger, Smoked Tofu and different types of herbs used for making Herb Tofu were analysed for their microbiological load. Tempeh samples consisted of cook water, inoculated beans and fresh tempeh. Fresh Tofu and Herb Tofu had high bacterial counts (10⁵ cfu/g). Tofu Burger and Smoked Tofu had counts around 10⁴ cfu/g. Tofu Paste and Fried Tofu had low counts (<10² cfu/g). Cutting board, knife and frequent hand contact with product were the major sources of contamination for tofu. Herbs used for making Herb Tofu were highly contaminated (>10⁵ cfu/g). Cook water and cooked beans for tempeh production had lower counts (10⁴ cfu/g). Initial count of fresh tempeh was high (about 10⁸ cfu/g). Most products had counts of >10⁶ cfu/g after 7 days of cold storage. The microbiological quality of the product may improve if producers avoid unnecessary contamination during processing.     

Biological and chemical evaluation of chick pea seed proteins as affected by germination,extraction and α-amylase treatment

The effects of germination, extraction (double extraction with 70% ethanol and water at isoelectric point) and -amylase treatments of chick pea seed flours on crude protein, total carbohydrate, protein efficiency ratio (PER), biological value (BV), true digestibility (TD), net protein utilization (NPU), essential amino acid composition, in-vitro protein digestibility (IVPD) and actual amino acid indices (essential amino acid index or amino acid score) were evaluated. Crude protein content was increased (8–149%), while total carbohydrate was decreased (11–62%) by germination, extraction and -amylase treatments. Alpha-amylase treatment was more efficient in reducing total carbohydrate and increasing the protein content than that of extraction treatment. The protein quality of chick pea flours as measured by PER, BV, TD, NPU, IVPD and corrected amino acid indices (actual amino acid indices×IVPD) was significantly improved by these treatments. The protein quality of germinated--amylase treatment was comparble with casein, while germinated--amylase treaded seeds appeared nutritionally superior to casein. The results indicate that the germinated--amylase and germinated--amylase-extracted treatments could be used successfully as a source of concentrated high quality protein for baby food production. The corrected amino acid indices gave better prediction of PER, BV, TD and NPU (r=93 to 97) than actual amino acid indices (r=45 to 71). PER was highly correlated with corrected amino acid score (r=0.93). The PER could be predicted from the following simple regression equation: PER=–1.827+0.0561×corrected amino acid score.     

Production,nutritional profiles and storability of ‘aadun’ and ‘kokoro’: Two corn products

Aadun and kokoro were produced from maize grains by processes involving roasting, kneading, spicing, and frying. Aadun with a moisture content (MC) of 12.5%, pH 5.3 had the following composition: crude protein, 7.49%; fat, 28.5%; total carbohydrate, 60.0%; ash, 3.0% and energy value, 3,600 kcal/kg whilst kokoro with a MC of 16% and pH 5.1 had, respectively: 6.42%; 22.9%; 66.6%; 2.84% and 3,550 kcal/kg. Whilst aadun and kokoro stored under ambient conditions (28±2°C; 75–80% RH) became mouldy within 7 days, samples treated with 1% butyl paraben did not develop off-colour and mouldiness.     

Influence of grain quality,heat, and processing time on the reduction of aflatoxin B1 levels in ‘tuwo’ and ‘ogi’: Two cereal-based products

During the production of tuwo from laboratory-contaminated corn (AFB₁:150 mcg/kg) and sorghum (AFB₁:87.5 mcg/kg) grains, reductions in the aflatoxin-B₁ levels of pastes boiled for 30 min and 60 min were found to be 68.0% and 80.8%, respectively. In the preparation of ogi from contaminated corn and sorghum grains, reductions of about 72.5% and 71.4%, respectively, were obtained after fermentation at ambient conditions. Reconstitution of ogi paste into a porridge (akamu) considerably reduced the AFB level.     

Zusammenhänge zwischen den für die Blaufleckigkeit der Kartoffelknolle verantwortlichen Inhaltsstoffen

Zusammenfassung Mit der Faktorenanlyse nach der Hauptachsenmethode und der aufbauenden multiplen Regressionsanalyse, unter Einbeziehung von 56 Einflußgrößen, konnte gezeigt werden, daß die Blaufleckigkeit von Kartoffelknollen im wesentlichen vom Gehalt an Trockensubstanz, -Aminobuttersäure, Lysin, Isochlorogensäure und den Mineralstoffen Ca und K abhängt. Eine kausale Erklärung des gesamten Komplexes Blaufleckigkeit ist allerdings zum gegenwärtigen Zeitpunkt noch nicht möglich.

---

Relationships between black spot of potatoes and several constituents of the tuber. With a factorial analysis and a multiple regression analysis could be shown that from 56 investigated constituents of the potato tuber only the dry matter content, aminobutyric acid, lysine, isochlorogenic acid, and potassium and calcium content influence the appearance of black spots.A causal explanation of the black spot phenomenon at present is not possible. Further investigations are necessary.

---

Résumé Relations entre la tâche noire des pommes de terre et divers constituants du tubercule.L'analyse factorielle, l'analyse de la régression multiple ont pu démontrer que sur 56 constituants déterminés dans le tubercule de pomme de terre seuls l'acide aminobutyrique, la lysine, l'acide isochlorogénique, le taux de potassium et de calcium agissent sur la formation de la tâche noire.Une explication causale du phénomène tâche noire n'est pas encore possible pour l'instant. De nouvelles recherches sont nécessaires.

     

On determination of chitin content in mushrooms

Nitrogen compounds constitute the greatest proportion of the dry weight of mushrooms. These nitrogen compounds are partly protein and partly non-protein. A modified Heneberg-Stohman's method was used to separate non-protein from protein nitrogen, as well as to determine raw fiber content in mushrooms, since the methods used previously for this purpose are time-consuming and complicated. Elemental analysis of crude fiber was performed to confirm the uniformity of isolated products. The content of total crude fiber (fungin) and the proportion of chitin and cellulose in it were determined. The chitin content of 15 species of field grown edible mushrooms was in the range of 1.87–6.93% of total dry mass and 34.51–67.72% of isolated fungin.     

Nutrient and antinutrient composition of three varieties ofPiper species

The proximate composition of three varieties ofPiper guineense (Odusa — Ibibio/Efik) viz. Uyat Odusa (cultivated and peppery), Eting-keni Ikot (wild forest variety) and Eting-keni mben inyang (wild, riverine variety), were determined using available standard methods. Also determined were mineral, antinutrient and ascorbate levels. The cultivated pepperic variety had the highest content of crude protein and moisture (18.9% and 97% respectively) while the wild, riverine variety had the highest content of ether extract, carbohydrate and calories (7.79%, 63.38% and 398 cals respectively). The cultivated variety had appreciable amounts of phosphorus (1.12 mg/100 g), potassium (1.2 mg/100 g), sodium (0.24 mg/100 g), zinc (0.18 mg/100 g), and copper (0.18 mg/100 g) while the forest variety contained more of calcium (12.38 mg/100 g), magnesium (1.21 mg/100 g) and iron (0.85 mg/100 g). The wild riverine variety appeared to have the least mineral content but had the highest ascorbate level of 173.4 mg/100 g. Of four antinutrients assayed, the cultivated pepperic one had the least quantities while the forest variety was highest in hydrocyanic acid (85.8 mg/100 g) and glucosinolates (0.20 mg/100 g). The wild riverine variety had the highest level of total oxalate (165.0 mg/100 g). These quantities are however far below documented toxic levels.     

Evaluation of turmeric accessions for quality

One hundred and eighty four accessions ofCurcuma were analysed for curing percentage (dry recovery), essential oil, oleoresin and curcumin contents. Curing percentage varied from 13.5 to 32.4. The cultivar Konni had the maximum percentage of oleoresin (19.2). The volatile oil content was more inC. aromatica than inC. domestica. Curcumin content varied from 2.3% in cultivar Hahim to 10.9% in cultivar Edapalayam. However, curcumin content was comparatively lower in six exotic types as well as in 14 related species. The importance of quality aspects with respect to breeding is discussed.Contribution No. 493 of Central Plantation Crops Research Institute, Kasaragod-670 124, Kerala, India.     

Traditional production and chemical composition ofNdaleyi,a Nigerian fermented pearl millet food

Ndaleyi, a fermented, sun dried agglomerated powder produced from pearl millet or sorghum is one of the most popular foods consumed in Nigeria, mostly by the Kanuri people of Borno State. Its traditional production is described. The mean yields ofndaleyi (mainly starch),chir (millet gluten) and bran (overtail orbina) were 30.6, 22.5 and 21.5%, recpectively. A mean yield loss of 20% was observed. Chemical analysis revealed thatchir and bran have higher protein, fat and ash contents thanndaleyi. The titratable acidities (as percent lactic acid) ofndaleyi andchir were 0.9 and 0.3, while their pH values were 3.3 and 3.5, respectively.     

Development and Acceptability of a Ready-to-Eat Β-Carotene Rich,Maize Based Supplementary Product

Low cost supplementary products using maize were developed and made using extrusion. -carotene rich sources like curry leaf, carrot, red palm oil were used at different level to increase vitamin A precursor Levels and, therefore, vitamin A. Incorporation of curry leaf powder and carrot powder at 30 percent level and 30:70 blend of red palm oil and groundnut oil were found to be more acceptable than the products made with other levels. These products were tasted for acceptability by preschool children and were analysed for energy, protein, fat and -carotene contents. The control, fresh and stored supplementary products contained 1.707, 1.922, 1.919 MJ, 11.0, 11.6, 10.36 g protein, 10.2, 10.4, 9.64 g fat, 0, 7.37,6.72 mg -carotene per 100g, respectively. The loss of -carotene in processing and storage of curry leaf and carrot supplemented products was 13.69, 6.25 and 20.24, 8.06 percent, respectively.     

Amylolytic-, trypsin-inhibiting-, and urease-activity in three varieties of soybeans and in soybean plant

Summary A comparative study has been carried out on amylase, urease and trypsin-inhibiting activities in the soybean varieties Adams, Harosoy and Lincoln and in soybean plant material of Harosoy. No significant quantitative difference was found between these activities in the three varieties. Two pH optima, at 5.5 and at 6.4 were found for amylase activity from all three varieties of soybeans. Although the temperature of 60° was most favourable for Adams-, Harosoy-and Lincoln-amylase, the latter lost almost all its activity at 70° whereas that of Adams remained constant.No urease and trypsin-inhibiting activities and only slight amylase activity could be detected in the leaves, stems and empty pods of the soybean plant. Young seeds (three weeks after setting) were compared, on the basis of dry weight, to mature seeds and were found to contain 50% of the trypsin inhibiting activity, 34% of the amylase and only 3% of the urease of the latter.

---

Zusammenfassung Eine vergleichende Studie wurde durchgeführt über Amylase-, Urease- und Trypsin-hemmende Wirkung der Sojabohnen-Sorten Adams, Harosoy und Lincoln sowie in Sojabohnenpflanzen der Sorte Harosoy. Keine mengenmäßig-signifikanten Unterschiede in der Wirkung fanden sich bei den drei Sorten. Zwei pH-Optima ergaben sich für die Amylase-Aktivität der drei Sojabohnen-Sorten bei 5,5 und 6,4. Obwohl die Temperatur von 60° sehr günstig für die Amylase der Sorten Adams, Harosoy und Lincoln war, verlor die Amylase der letztgenannten Sorte fast ihre gesamte Wirksamkeit bei 70°, während die der Sorte Adams dabei konstant blieb.Keine Urease- und Trypsin-hemmende Wirkung und eine nur leichte Amylase-Aktivität konnte in den Blättern, Sproßachsen und in den leeren Hülsen der Sojabohnen-Pflanze nachgewiesen werden. Junge Samen (drei Wochen nach der Aussaat) wurden auf Trockengewichtsbasis mit reifen Samen verglichen. Es wurde bei den jungen Samen — im Vergleich zu den reifen — eine nur 50%ige Trypsin-hemmende Wirkung, eine 34%ige der Amylase und eine nur 3%ige der Urease ermittelt.

---

Resumen Se ha efectuado un estudio comparativo sobre las actividades de la amilasa, la ureasa y el inhibidor de la tripsina en las variedades de porotos de soya Adams, Harosoy y Lincoln, así como en algunas partes vegetativas de la variedad Harosoy. No se encontraron ningunas diferencias cuantitativas entre las actividades de estas variedades. Se encontraron dos valores optimales del pH, 5.5 y 6.4, para la amilasa de todas las tres variedades de porotos de soya. Aunque la temperatura más favorable para la amilasa de las variedades Adams, Harosoy y Lincoln fuera 60°, esta última perdió casi toda su actividad a 70°, mientras que la actividad de la variedad Adams se quedó sin cambiar.No se pudo demostrar la presencia de ureasa y actividad inhibidora de la tripsina en las hojas, los tallos y las cápsulas vacías de las plantas de soya, y se encontró solamante una ligera actividad de amilasa en estas partes vegetativas. Se compararon, en base seca, semillas nuevas (a las tres semanas de formarse) con semillas maduras. Se encontró que las primeras exhibieron 50% de la actividad inhibidora de la tripsina, 34% de la actividad de amilasa y solo 3% de la actividad de ureasa, en comparación con las actividades de las semillas maduras.

---

Resumé Une étude comparative a été faite sur les activités de l'amylase, l'uréase et l'activité inhibitrice de la trypsine, dans les variétés de fèves de soya: Adams, Harosoy et Lincoln ainsi que sur différents stades de l'appareil végatatif du soya Harosoy. Aucune différence quantitative, significative, ne fut trouvée entre les activités dans les trois variétés. Deux pH optima à 5.5 et à 6.4 furent trouvés pour l'activité de l'amylase de toutes les trois variétés de fèves de soya. Bien que la témperature de 60° fut la plus favorable pour l'amylase de Adams, Harosoy et Lincoln, l'amylase de ces 2 derniers perdit presque toute son activité à 70° tandis que l'amylase dAdams resta constante.Ni activité d'urease, ni activité inhibitrice de trypsine ainsi que très peu d'amylase ne furent observés dans les feuilles, tiges et gousses vides de la plante soya. De jeunes semences (trois semaines après la fécondation) furent comparées, sur la base du poids sec, à des semences adultes et furent trouvées contenir 50% de l'activité inhibitrice de la trypsine, 34% de l'amylase et rien que 3% de l'uréase par rapport aux graines adultes.

---

---

with 3 figs.     

Physical and biochemical properties of green banana flour

Banana flour prepared from two cooking banana varieties, namely Alukehel and Monthan, were evaluated for their physical and biochemical characteristics. The yields of flour averaged 31.3% for Alukehel and 25.5% for Monthan. The pH of the flour ranged from 5.4 to 5.7. The bulk density and particle size distribution were also measured. The average chemical composition (% dry matter) of the flours were as follows: crude protein, 3.2; crude fat, 1.3; ash, 3.7; neutral detergent fiber, 8.9; acid detergent fiber, 3.8; cellulose, 3.1; lignin, 1.0 and hemicellulose, 5.0. Carbohydrate composition indicated the flour to contain 2.8% soluble sugars, 70.0% starch and 12.0% non-starch polysaccharides. Potassium is the predominant mineral in banana flour. Fresh green banana is a good source of vitamin C, but almost 65% is lost during the preparation of flour. Oxalate content (1.1–1.6%) of banana flour is probably nutritionally insignificant. The overall results are suggestive of the potential of green bananas as a source of flour.     

Qualitative and quantitative changes in the seed and oil content ofBrassica juncea mutants at different times of harvesting

With increasing maturity, the moisture percentage, a, b, and total chlorophyll and crude fibre content decreased progressively, whereas oil and protein content increased. However, 1000-grain weight and pod yield increased up to stage II. Some significant correlations observed in green pod stage I were absent in the pale yellow stage II and golden yellow stage III of maturity. The positive significant correlations between 1000-grain weight and seed yield existed in both stages I and II, but there was no correlation between these characters at the golden yellow stage III. Thus mustard can be harvested at the pale yellow pod stage without any adverse effect on seed quality and yield.Presented at the 15th Annual Workshop cum Seminar on rapeseed and mustard of the All-India Coordinated Research Project on Rabi oilseed crops held at CSA University of Agriculture and Technology, Kanpur, India, 12–13 September 1979.     

Effect of traditional open sun-drying and solar cabinet drying on carotene content and vitamin A activity of green leafy vegetables

Dried green leafy vegetables constitute a major dietary source of provitamin A carotenoids for people living in semi-arid areas of Tanzania. In the present study, eight vegetable varieties commonly consumed in these areas were either traditionally sun-dried or solar-dried, and the amount of provitamin A carotenoids were determined by HPLC. In the fresh blanched leaves, the contents of -carotene, -carotene, 9-cis--carotene and 13-cis--carotene were in the range of 526–917, 12–39, 80–136 and 16–40 g/g dry matter/basis; dmb, respectively. The average amounts of all-trans--carotene in the leaves after blanching, solar drying and open sun-drying were 662, 502 and 353 g/g dmb, respectively, all significantly different from each other (p <0.001). The average amounts of all-trans--carotene were 21, 15 and 8 g/g dmb (p <0.05) and of 9-cis--carotene 101, 82 and 63 g/g dmb (p <0.05) in the blanched, solar-dried and open sun-dried samples, respectively. Estimation of the amount of retinol equivalents provided by the dried vegetables for preparation of an edible portion of 100 g relish showed that solar-dried vegetables contributed more (between 120–275%) than the recommended level of vitamin A, and on average 43% more retinol equivalents than relishes of open sun-dried vegetables.Thus, it is concluded that the amount of provitamin A-carotenes in traditionally treated vegetables were highly reduced by open sun-drying while solar drying resulted in significantly more retention of the carotenes. All-trans--carotene and 9-cis--carotene were similarly affected as all-trans--carotene by the two drying methods. Consumption of a 100 g portion of solar-dried vegetable relish could provide the recommended daily intake of vitamin A.     

Production of farnesene and incidence of scald in stored apples

Superficial scald is a physiological disorder of stored apples. A review is given of the symptoms of the disease, the many factors which influence its severity and of the evidence for a volatile causative agent.-Farnesene, an unusual terpene hydrocarbon present in apple wax, may be involved in scald. It is found to be produced in increasing quantities at the time of the climacteric.When farnesene was applied to apples before storage, the analysis indicated that rapid oxidation had occurred. The incidence of scald was unaffected.

---

Zusammenfassung Die Hautbräune ist eine physiologische Störung bei gelagerten Äpfeln. Eine Übersicht wird gegeben über die Symptome der Krankheit, über die vielen Faktoren, die ihren Schweregrad beeinflussen sowie über die offensichtliche Gegenwart eines dafür verantwortlichen, flüchtigen Agens.-Farnesen, ein üblicherweise im Apfelwachs nicht vorkommender Terpen-Kohlenwasserstoff, kann an der Entstehung der Hautbräune beteiligt sein. Es wurde gefunden, dass es in steigenden Mengen zur Zeit der Nachreife entsteht.Wurde Farnesen Äpflen vor der Lagerung appliziert, ergaben die Analysen, dass eine schnelle Oxidation eingetreten war. Die Entstehung der Hautbräune wurde nicht beeinflusst.

---

Resume Le scald superficiel (échaudure) consiste en un désordre physiologique apparaissant chez les pommes en conservation. On présente une étude des symptômes de la maladie, des facteurs qui peuvent influencer sa gravité, ainsi que d'un agent volatile responsable de son apparition.L'-farnésène, un hydrocarbure terpénique rare, présent dans la cire de la pomme, peut être impliqué dans le scald. On a trouvé que cet hydrocarbure est produit en quantités croissantes au moment du climactérique. Lorsque du farnésène est appliqué à des pommes avant leur conservation, l'analyse indique une oxydation rapide. L'apparition du scald n'est pas affectée dans ce cas.

     

Some chemical and biological properties of a protein concentrate from nigerseed (Guizotia abyssinica Cass.)

A protein concentrate has been prepared from nigerseed (Guizotia abyssinica Cass.) by a method involving extraction of the protein with NaCl-solution.The protein concentrate contains about 65% of protein (N×5.5) calculated from the dry weight, and the amino acid pattern is fairly well balanced. The chemical score is 82 calculated from the A/E values.The nutritional value of the product has been tested on young rats. These tests revealed the presence of antinutritional factors in the protein concentrate. The PER was only 1.5.It was also found that the rate of digestion by pancreatin was much slower for the protein concentrate than for casein, possibly due to enzyme resistent linkages.The protein concentrate seems to be devoid of hemagglutinin activity. Antibodies against the nug extracts could not be found.

---

Zusammenfassung Ein Proteinkonzentrat wurde aus Nug-Samen (Guizotia abyssinica Cass.) durch eine Methode gewonnen, die eine Extraktion des Proteins mit NaCl-Lösung einschließt.Das Proteinkonzentrat enthalt etwa 65% Protein (N x 5,5), berechnet auf das Trockengewicht. Das Aminosäuregleichgewicht ist relativ gut balanziert. Die chemical score nach den A/E Werten berechnet, beträgt 82.Der Nährwert des Produktes ist an jungen Ratten getestet worden. Diese Tests zeigten das Vorhandensein von Antinähr-Faktoren im Proteinkonzentrat. Die PER (protein efficiency ratio) betrug nur 1,5.Es wurde außerdem festgestellt, daß die Geschwindigkeit der enzymatischen Verdauung (mit Pankreatin) für das Proteinkonzentrat viel langsamer abläuft als für Casein. Dieses ist vielleicht bedingt durch Enzymresistente Bindungen.Das Proteinkonzentrat scheint von Hämagglutininaktivität frei zu sein. Es wurden keine Antikörper gegen Nug-Extrakte gefunden.

---

Résumé Une solution concentrée de protéine a été préparée de grains de nug (Guizotia abyssinica Cass.) par une méthode contenant l'extraction de la protéine avec la solution NaCl.La solution concentrée contient environ 65% de protéine (N X 5,5) calculée du poids sec, et le modèle des aminoacides est assez bien balancé. Le chemical score est 82 calculé des valeurs A/E. La valeur nutritive du produit a été éprouvé à de jeunes rats. Ces épreuves ont révélé la présence d'éléments antinutritives dans la solution concentrée de protéine. La PER (protein efficiency ratio) était seulement 1,5.On a aussi trouvé que la rapidité de digestion enzymatique (avec la pancréatine) pour la solution concentrée de protéine était beaucoup plus lente que pour le casein. Ceci est peut-être du à des liaisons qui résistent aux enzymes.Il semble que la solution concentrée de protéine manque d'activité hemagglutinine. On n'a pas pu trouver des anticorps dirigés contre les extraits de nug.

---

---

CNU Report No. 34.     

Inhibition of α-amylase purified fromOctopus vulgaris Lam. by albumin inhibitors from wheat flour

Octopus -amylase has been purified to homogeneity by single-step affinity chromatography on Sepharose-bound wheat albumin inhibitors. Two electrophoretically distinguishable isoamylases are obtained, both consisting of a single polypeptide chain with molecular weight 45,000. Octopus -amylase is more effectively inhibited by a monomeric than by a dimeric protein inhibitor from wheat. The inhibition is dependent on pH, temperature, and time of preincubation. Properties are compared with those of other animal -amylases.