Changes in the physiology and feed quality of cocksfoot (Dactylis glomerata L.) during regrowth

Abstract A glasshouse study was undertaken to determine the physiological and morphological changes in cocksfoot (Dactylis glomerata L.) during regrowth after defoliation. Individual plants were arranged in a mini‐sward in a randomized complete block design. Treatments involved harvesting each time one new leaf had expanded (one‐leaf stage), up to the six‐leaf stage, with the plants separated into leaf, stubble (tiller bases) and roots. Stubble and root water‐soluble carbohydrate (WSC), stubble and leaf dry matter (DM), tiller number per plant and leaf quality (crude protein (CP), estimated metabolizable energy (ME) and mineral content) were measured to develop optimal defoliation management of cocksfoot‐based pastures. WSC concentration in stubble and roots was highest at the five‐ and six‐leaf stages. Mean WSC concentration (g kg^?1 DM) was greater in stubble than roots (32·7 ± 5·9 vs. 9·4 ± 1·5 respectively). There was a strong positive linear relationship between plant WSC concentration and leaf DM, root DM and tillers per plant after defoliation (Adj R² = 0·72, 0·88 and 0·95 respectively). Root DM plant^?1 and tiller DM tiller^?1 decreased immediately following defoliation and remained low until the three‐leaf stage, then increased from the four‐leaf stage. Tillers per plant remained stable until the four‐leaf stage, after which they increased (from 9·9 ± 0·5 to 15·7 ± 1·0 tillers plant^?1). Estimated metabolizable energy concentration (MJ kg^?1 DM) was significantly lower at the six‐leaf stage (11·01 ± 0·06) than at any previous leaf regrowth stage, whereas CP concentration (g kg^?1 DM) decreased with regrowth to the six‐leaf stage. Both the levels of ME and CP concentrations were indicative of a high quality forage throughout regrowth (11·37 ± 0·04 and 279 ± 8·0 for ME and CP respectively). Results from this study give a basis for determining appropriate criteria for grazing cocksfoot‐based pastures. The optimal defoliation interval for cocksfoot appears to be between the four‐ and five‐leaf stages of regrowth. Delaying defoliation to the four‐leaf stage allows time for replenishment of WSC reserves, resumption of root growth and an increase in tillering, and is before herbage is lost and quality falls due to onset of leaf senescence.     

The impact of defoliation frequency and nitrogen fertilizer application in spring on summer survival of perennial ryegrass under grazing in subtropical Australia

Abstract This field study investigated the effect of timing of nitrogen (N) fertilizer application in spring on the survival of grazed perennial ryegrass (Lolium perenne cv. Dobson and Yatsyn) over summer in a subtropical environment. There were five N fertilizer treatments: no applied N, 46 kg N ha^?1 on 22 October or 22 November or 22 December, or on 22 October and again on 22 December. Water‐soluble carbohydrate (WSC) concentration of perennial ryegrass plants entering the summer was altered by varying defoliation frequency, with defoliation interval based on the number of leaves per tiller. Frequent defoliation was set at a regrowth level of one leaf per tiller and less frequent defoliation at a regrowth level of three leaves per tiller, over a total of two by three‐leaf per tiller regrowth periods. Application of N fertilizer was found to have no significant effect (P > 0·05) on survival of perennial ryegrass plants over summer. On the other hand, defoliation had a marked effect on perennial ryegrass persistence, with frequent defoliation decreasing ryegrass plant density (51 vs. 88 plants m^?2; P < 0·001) and increasing the density of tropical weed grasses (99 vs. 73 plants m^?2; P < 0·001) by autumn. Frequently defoliated plants had a lower stubble WSC content on a per plant basis than less frequently defoliated plants in spring (103 vs. 201 mg per plant; P < 0·001) and summer (59 vs. 101 mg per plant; P < 0·001). The lower WSC content was associated with a smaller root system in spring (1·50 vs. 2·14 g per plant; P < 0·001) and autumn (1·79 vs. 2·66 g per plant; P < 0·01), and this was reflected in 0·29 more plants being pulled from the soil by livestock between November 1996 and April 1997. Rhizoctonia fungus was associated with roots of pulled plants, but not with roots of seemingly healthy plants, indicating that this fungus may have a role in a weakened root system, which was more prone to sod pulling. Nitrogen applied in October and November resulted in a reduced WSC concentration, although the effect was restricted to 1 month after N application. The present study indicates that survival of perennial ryegrass plants over the summer in a subtropical region is prejudiced by frequent defoliation, which is associated with a lower WSC concentration and a shallower root system. Under grazing, sod pulling is a reflection of this weaker root system and contributes to plant mortality.     

Response of herbage regrowth and water-soluble carbohydrate concentration of ryegrass species to defoliation practices when grown in a Mediterranean environment

Three experiments were conducted to determine the association between leaf number per tiller at defoliation, water‐soluble carbohydrate (WSC) concentration and herbage mass of juvenile ryegrass plants when grown in a Mediterranean environment. Seedlings of ryegrass were grown in nursery pots arranged side‐by‐side and located outside in the open‐air to simulate a mini‐sward in Experiments 1 and 2, and a mixture of annual ryegrass and subterranean clover (Trifolium subterraneum L.) was grown in a small plot field study in Experiment 3. Swards were defoliated mechanically with the onset of defoliation commencing within 28 d of germination. Frequency of defoliation ranged from one to nine leaves per tiller, whilst defoliation height ranged from 30 mm of pseudostem height that removed all leaf laminae in Experiment 1, to 50 mm of pseudostem height with some leaf laminae remaining post‐defoliation in Experiments 2 and 3. A positive relationship between herbage mass of ryegrass, WSC concentration and leaf number per tiller at defoliation was demonstrated in all experiments. In Experiment 1, the herbage mass of leaf, pseudostem and roots of tillers defoliated at one leaf per tiller was reduced to 0·10, 0·09 and 0·06 of those tillers defoliated less frequently at six leaves per tiller. However, the reduction in herbage mass from frequent defoliation was less severe in Experiment 2 and coincided with a 0·20 reduction in WSC concentration of pseudostem compared with 0·80 measured during Experiment 1. In Experiment 3, the highest harvested herbage mass of ryegrass occurred when defoliation was nine leaves per tiller. Although the harvested herbage from this sward contained senescent herbage, the in vitro dry‐matter digestibility of the harvested herbage did not differ significantly compared with the remaining treatments that had been defoliated more frequently. Leaf numbers of newly germinated ryegrass tillers in a Mediterranean environment were positively associated with WSC concentration of pseudostem and herbage mass. A minimum period of two to three leaf appearances was required to restore WSC concentrations to levels measured prior to defoliation thereby avoiding a significant reduction in herbage mass. However, maximum herbage mass of a mixed sward containing ryegrass and subterranean clover was achieved when defoliation was delayed to nine leaves per tiller.     

Effect of defoliation management, based on leaf stage, on perennial ryegrass (Lolium perenne L.), prairie grass (Bromus willdenowii Kunth.) and cocksfoot (Dactylis glomerata L.) under dryland conditions. 1. Regrowth, tillering and water-soluble carbohydrate concentration

A field study was undertaken between April 2003 and May 2004 in southern Tasmania, Australia to quantify and compare changes in herbage productivity and water‐soluble carbohydrate (WSC) concentration of perennial ryegrass (Lolium perenne L.), prairie grass (Bromus willdenowii Kunth.) and cocksfoot (Dactylis glomerata L.) under a defoliation regime based on leaf regrowth stage. Defoliation interval was based on the time taken for two, three or four leaves per tiller to fully expand. Dry‐matter (DM) production and botanical composition were measured at every defoliation event; plant density, DM production per tiller, tiller numbers per plant and WSC concentration were measured bimonthly; and tiller initiation and death rates were monitored every 3 weeks. Species and defoliation interval had a significant effect (P < 0·05) on seasonal DM production. Prairie grass produced significantly more (P < 0·001) DM than cocksfoot and ryegrass (5·7 vs. 4·1 and 4·3 t DM ha^?1 respectively). Plants defoliated at the two‐leaf stage of regrowth produced significantly less DM than plants defoliated at the three‐ and four‐leaf stages, irrespective of species. Defoliation interval had no effect on plant persistence of any species during the first year of establishment, as measured by plant density and tiller number. However, more frequent defoliation was detrimental to the productivity of all species, most likely because of decreased WSC reserves. Results from this study confirmed that to maximize rates of regrowth, the recommended defoliation interval for prairie grass and cocksfoot is the four‐leaf stage, and for perennial ryegrass between the two and three‐leaf stages.     

Effect of grazing management on herbage protein concentration,milk production and nitrogen excretion of dairy cows in mid‐lactation

The objective of this experiment was to use diurnal and temporal changes in herbage composition to create two pasture diets with contrasting ratios of water‐soluble carbohydrate (WSC) and crude protein (CP) and compare milk production and nitrogen‐use efficiency (NUE) of dairy cows. A grazing experiment using thirty‐six mid‐lactation Friesian x Jersey cows was conducted in late spring in Canterbury, New Zealand. Cows were offered mixed perennial ryegrass and white clover pastures either in the morning after a short 19‐day regrowth interval (SR AM) or in the afternoon after a long 35‐day regrowth interval (LR PM). Pasture treatments resulted in lower pasture mass and greater herbage CP concentration (187 vs. 171 g kg^?1 DM) in the SR AM compared with the LR PM but did not affect WSC (169 g kg^?1 DM) or the ratio of WSC/CP (1·0 g g^?1). Cows had similar apparent DM (17·5 kg DM cow^?1 d^?1) and N (501 g N cow^?1 d^?1) intake for both treatments. Compared with SR AM cows, LR PM cows had lower milk (18·5 vs. 21·2 kg cow^?1 d^?1), milk protein (0·69 vs. 0·81 kg cow^?1 d^?1) and milk solids (1·72 and 1·89 kg cow^?1 d^?1) yield. Urinary N concentration was increased in SR AM, but estimated N excretion and NUE for milk were similar for both treatments. Further studies are required to determine the effect of feeding times on diurnal variation in urine volume and N concentration under grazing to predict urination events with highest leaching risk.     

Effect of defoliation management, based on leaf stage, on perennial ryegrass (Lolium perenne L.), prairie grass (Bromus willdenowii Kunth.) and cocksfoot (Dactylis glomerata L.) under dryland conditions. 2. Nutritive value

A field experiment was undertaken between April 2003 and May 2004 in southern Tasmania, Australia, to quantify and compare changes in the nutritive value of perennial ryegrass (Lolium perenne L.), prairie grass (Bromus willdenowii Kunth.) and cocksfoot (Dactylis glomerata L.) under a defoliation regime based on stage of leaf regrowth. Defoliation interval was based on the time taken for two, three or four leaves per tiller to fully expand. At every defoliation event, samples were collected and analysed for acid‐detergent fibre (ADF), neutral‐detergent fibre (NDF) and total nitrogen (N) concentrations and to estimate metabolizable energy (ME) and digestible dry matter (DDM) concentrations. Amounts of crude protein (CP) and metabolizable energy (MJ) per hectare values were subsequently calculated. There was a significantly lower (P < 0·001) NDF concentration for perennial ryegrass compared with prairie grass and cocksfoot, and a significantly lower (P < 0·001) ADF concentration for cocksfoot compared with prairie grass and perennial ryegrass, regardless of defoliation interval. The CP concentration of cocksfoot was significantly greater (P < 0·001) compared with the CP concentrations of prairie grass and perennial ryegrass. The estimated ME concentrations in cocksfoot were high enough to satisfy the requirements of a lactating dairy cow, with defoliation at or before the four‐leaf stage maintaining ME concentrations between 10·7 and 10·9 MJ kg^?1 DM, and minimizing reproductive plant development. The ME concentrations of prairie grass (10·2–10·4 MJ kg^?1 DM) were significantly lower (P < 0·001) than for cocksfoot (as above) and perennial ryegrass (11·4–11·6 MJ kg^?1 DM) but a higher DM production per hectare resulted in prairie grass providing the greatest amounts of ME ha^?1.     

Modelling the concentrations of nitrogen and water-soluble carbohydrates in grass herbage ingested by cattle under strip-grazing management

There is scope of increasing the nitrogen (N) efficiency of grazing cattle through manipulation of the energy and N concentrations in the herbage ingested. Because of asymmetric grazing by cattle between individual plant parts, it has not yet been established how this translates into the concentrations of N and water‐soluble carbohydrates (WSC) in the herbage ingested. A model is described with the objective of assessing the efficacy of individual tools in grassland management in manipulating the WSC and N concentrations of the herbage ingested by cattle under strip‐grazing management throughout the growing season. The model was calibrated and independently evaluated for early (April), mid‐ (June, regrowth phase) and late (September) parts of the growing season. There was a high correlation between predicted and observed WSC concentrations in the ingested herbage (R² = 0·78, P < 0·001). The correlation between predicted and observed neutral‐detergent fibre (NDF) concentrations in the ingested herbage was lower (R² = 0·49, P < 0·05) with a small absolute bias. Differences in the N concentration between laminae and sheaths, and between clean patches and fouled patches, were adequately simulated and it was concluded that the model could be used to assess the efficacy of grassland management tools for manipulating the WSC and N concentrations in the ingested herbage. Model application showed that reduced rates of application of N fertilizer and longer rotation lengths were effective tools for manipulating herbage quality in early and mid‐season. During the later part of the growing season, the large proportion of area affected by dung and urine reduced the effect of application rate of N fertilizer on herbage quality. In contrast, relative differences between high‐sugar and low‐sugar cultivars of perennial ryegrass were largest during this period. This suggests that high‐sugar cultivars may be an important tool in increasing N efficiency by cattle when risks of N losses to water bodies are largest. The model output showed that defoliation height affects the chemical composition of the ingested herbage of both the current and the subsequent grazing period.     

The effects of defoliating grass in winter or spring on herbage yields and ensilage characteristics

Under Irish conditions, the digestibility in May of grass managed for silage production is sometimes lower than expected. In each of two successive years, replicate field plots were established to examine the effects of three defoliation heights (uncut or cut to a stubble height of 10 or 5 cm) applied in winter and/or spring on herbage yields harvested in May and again in July, and on chemical composition and conservation characteristics associated with first‐cut silage. Swards that were not defoliated in December or March had a dry‐matter (DM) yield and in vitro DM digestibility (DMD) in mid‐May of 6597 kg ha^?1 and 736 g kg^?1, respectively, in Year 1, and corresponding values of 7338 kg ha^?1 and 771 g kg^?1 in Year 2. Defoliating swards to 5 cm in December reduced (P < 0·001) May DM yields compared to swards that were not defoliated in both December and March, while herbage DMD in May increased (P < 0·001) when defoliated in December or March. There were no clear effects of defoliation height or its timing on herbage ensilability or resultant conservation efficiency characteristics. The effects of defoliation on July yield were the reverse of those observed for May, while the total yield of the December and March defoliations plus the two silage harvests increased as defoliation height was lowered in Year 2 only. It is concluded that defoliation in winter and/or spring can increase herbage digestibility but will likely reduce DM yields in May.     

The effects of rate and timing of application of fertilizer nitrogen in late summer on herbage mass and chemical composition of perennial ryegrass swards over the winter period in Northern Ireland

A small‐plot experiment was carried out in Northern Ireland on a predominantly perennial ryegrass sward over the period July 1993 to March 1994 to investigate the effect of timing and rate of fertilizer nitrogen (N) application on herbage mass and its chemical composition over the winter period. Eighty treatment combinations, involving four N fertilizer application dates (28 July, 9 and 30 August and 20 September 1993), four rates of N fertilizer (0, 30, 60 and 90 kg N ha^?1) and five harvest dates (1 October, 1 November, 1 December 1993, 1 February and 1 March 1994), were replicated three times in a randomized block design experiment. N application increased herbage mass at each of the harvest dates, but in general there was a decrease in response to N with increasing rate of N and delay in time of application. Mean responses to N applications were 13·0, 11·5 and 9·5 kg DM kg^?1 N at 30, 60 and 90 kg N ha^?1 respectively. Delaying N application, which also reduced the length of the period of growth, reduced the mean response to N fertilizer from 14·3 to 7·4 kg DM kg^?1 N for N applied on 28 July and 20 September respectively. Increasing rate of N application increased the N concentration and reduced the dry‐matter (DM) content and water‐soluble carbohydrate (WSC) concentration of the herbage but had little effect on the acid‐detergent fibre (ADF) concentration. Delaying N application increased N concentration and reduced DM content of the herbage. The effect of date of N application on WSC concentration varied between harvests. A decrease in herbage mass occurred from November onwards which was associated with a decrease in the proportion of live leaf and stem material and an increase in the proportion of dead material in the sward. It is concluded that there is considerable potential to increase the herbage mass available for autumn/early winter grazing by applying up to 60 kg N ha^?1 in early September.     

Effects of continuous or rotational grazing of two perennial ryegrass varieties on the chemical composition of the herbage and the performance of finishing lambs

Plant breeding has developed perennial ryegrass varieties with increased concentrations of water‐soluble carbohydrates (WSCs) compared with conventional varieties. Water‐soluble carbohydrates are major metabolic and storage components in ryegrass. Therefore, if perennial ryegrass herbage is allowed to grow to greater heights it should contain higher water‐soluble carbohydrates concentrations, for example as under rotational grazing rather than continuous grazing by livestock. This study investigated this hypothesis and measured the performance of lambs grazed rotationally and continuously. Replicated plots of the variety AberDart (bred to express high WSC concentrations) or the variety Fennema were grazed by a core group of ten male Cheviot lambs for 10 weeks. Lambs were weighed and replicate forage samples were taken every 7 d. Concentrations of WSC in AberDart herbage were significantly (P < 0·05), but not substantially, higher than those in Fennema herbage. Rotational grazing did not increase the differential in WSC concentration between the AberDart and Fennema varieties. However, there was a tendency (P = 0·07) for lambs rotationally grazing the AberDart swards to have a higher final live weight than lambs grazing the Fennema swards. Overall, lamb performance was increased when either perennial ryegrass variety was rotationally rather than continuously grazed (P < 0·001).     

In vitro total and methane gas production as influenced by rate of nitrogen application, season of harvest and perennial ryegrass cultivar

The effects of rate of inorganic nitrogen (N) fertilization (0, 80 or 160 kg N ha^?1 per regrowth), season of harvest (regrowths 1, 2 and 3) and perennial ryegrass (Lolium perenne L.) cultivar [classified as having either a normal or elevated water soluble carbohydrate (WSC) concentration genotype] on in vitro gas production and digestibility were assessed. Increased N fertilizer application significantly decreased total gas production (TGP), methane (CH₄) production and organic matter digestibility (OMD). The results suggest that the decreases in TGP and CH₄ production were associated with a restriction in organic matter (OM) fermentation and an altered crude protein (CP) to structural carbohydrate ratio rather than a modification in the stoichiometry of fermentation. Season of harvest only significantly (P < 0·05) altered in vitro OMD and CH₄ production at 8 h, despite altering the chemical composition of the herbage. Cultivar effects on all measured in vitro parameters were not significant presumably because the elevated WSC concentration trait was not expressed strongly in the study.     

Nitrogen recovery and loss in a fertilized elephant grass pasture

Limited information is available regarding the recovery and loss of fertilizer nitrogen (N) applied to intensively managed tropical grass pastures. An experiment was carried out in Brazil to determine the fertilizer‐N recovery and ammonia volatilization loss in an elephant grass (Pennisetum purpureum, Schum.) pasture fertilized with 100 kg N ha^?1 as urea or ammonium sulphate, labelled with ¹⁵N, in late summer (LS) or in mid‐autumn (MA). Herbage mass was highest and litter mass was lowest in LS (P < 0·05). The N concentration of herbage was highest in autumn (P < 0·05) and the total N content in soil was lower in LS than in MA (P < 0·05), reflecting the high N uptake capacity of the grass. Proportionately higher ¹⁵N recovery in litter mass (P < 0·05) was observed in autumn (0·094) than in LS (0·0397) and the ¹⁵N recovery in herbage was 0·046 higher for ammonium sulphate‐fertilized pastures (P < 0·05; proportionately 0·243 for ammonium sulphate and 0·197 for urea). Around 0·60 of the fertilizer‐¹⁵N recovered was retained in soil and in non‐harvestable fractions of the plant. The NH₃ volatilization loss was higher in LS and most of the N loss occurred soon after fertilizer application. Urea and ammonium sulphate fertilizers were equally effective in sustaining herbage dry matter yield in the short term. However, the use of ammonium sulphate, rather than urea, would be preferable for LS applications when the objective is to reduce NH₃ volatilization losses.     

The relative impacts of wilting, chopping, compaction and air infiltration on the conservation characteristics of ensiled grass

The chemical composition of baled silage frequently differs from that of comparable conventional silage. The extents of wilting, chopping, compaction and air infiltration potentially contribute to these differences in conservation characteristics. An experiment was organized in a 3 (0, 24 or 48‐h wilting to influence herbage dry‐matter content) × 2 (unchopped or chopped) × 2 (with or without compaction) × 2 (with or without air infiltration) factorial arrangement of treatments, to elucidate the relative effects of these factors on the conservation characteristics of ensiled grass. Dry‐matter content of herbage and infiltration of air had a greater effect on silage conservation characteristics than chopping and compaction. The main interactions were between wilting and air infiltration, wilting and compaction, and compaction and air infiltration. Air infiltration stimulated a secondary fermentation in the unwilted herbage, reflected in a large increase (P < 0·001) in clostridial activity. As wilting progressed, air infiltration facilitated yeast respiration of water‐soluble carbohydrates (WSC) and resulted in an increase (P < 0·001) in in‐silo fresh‐weight losses. Compaction reduced (P < 0·05) silage pore space and, as a result, the extent to which air could penetrate the silage mass. Compaction of the wilted herbage restricted respiration and was reflected in increased (P < 0·05) concentrations of WSC and in a reduction (P < 0·001) in fresh‐weight loss. The rapid achievement and maintenance of adequately anaerobic conditions is the primary requirement for baled silage. This study showed that failure to achieve this will lead to progressively greater losses, especially with drier herbage.     

Priority for allocation of water-soluble carbohydrate reserves during regrowth of Lolium perenne

A glasshouse study was undertaken to determine the priority within the perennial ryegrass (Lolium perenne L.) plant for leaf and root growth and daughter tiller initiation after defoliation, in relation to various levels of water-soluble carbohydrate (WSC) reserves at defoliation. Individual plants were arranged in mini-swards, and underwent varying defoliation frequencies and ambient temperatures before defoliation, and harvest heights at defoliation, to obtain a gradient of WSC content at H₁, the date when all plants were defoliated. Defoliation interval consisted of defoliating either three times at the one new leaf tiller^–1 stage (1-leaf stage) of regrowth, or once only at the 3-leaf stage, up to H₁, while night temperature in the week prior to H₁ was altered from 15°C to either 8 or 20°C. At H₁, plants were defoliated to a stubble height of either 20 or 50 mm. Plants were subsequently destructively harvested at days 4, 6, 8, 12, 18 and 27. Leaf and root extension and tiller dynamics were also measured. On a regrowth timescale, tiller initiation was most sensitive, root regrowth moderately sensitive, and leaf regrowth relatively insensitive to a decrease in WSC. The time of daughter tiller initiation also coincided with replenishment of stubble WSC levels. In contrast to this sequence of regrowth events following defoliation, the quantitative effects on growth were different, with elongation and survival of roots most affected by reduced WSC levels. A 30-fold difference in stubble WSC at H₁ between high and low WSC plants (1·52 vs. 0·05 mg tiller^–1) produced only a 4-fold increase in leaf dry matter (DM) after 27 d (2·2 vs. 0·6 g plant^?1), while tiller number plant^?1 increased 6-fold (138 vs. 23% increase in tiller number from H₁). Root elongation rate was 59 times higher in the high than in the low WSC plants (1·18 vs. 0·02 mm d^?1). From a pasture management perspective, the study confirms that defoliation, coinciding with the 3-leaf stage of regrowth and around a stubble height of 50 mm, optimizes persistence and productivity of perennial ryegrass. By allowing more rapid replenishment of WSC reserves, this optimal defoliation strategy enables a greater proportion of WSC to be allocated to maintain a more active root system, and promotes tillering, compared with more frequent and close defoliation.     

The effect of initial spring grazing date and subsequent stocking rate on the grazing management, grass dry matter intake and milk production of dairy cows in summer

The objective of this study was to investigate the effects of an early (February; F) or delayed (April; A) primary spring grazing date and two stocking rates, high (H) and medium (M), on the grazing management, dry matter (DM) intake of grass herbage and milk production of spring‐calving dairy cows grazing a perennial ryegrass sward in the subsequent summer. Sixty‐four Holstein‐Friesian dairy cows (mean of 58 d in milk) were assigned to one of four grazing treatments (n = 16) which were imposed from 12 April to 3 July 2004. Cows on the early spring‐grazing treatment were grazed at 5·5 cows ha^?1 (treatment FH) and 4·5 cows ha^?1 (treatment FM) while cows on the late‐grazing treatment were grazed at 6·4 cows ha^?1 (treatment AH) and 5·5 cows ha^?1 (treatment AM). The organic matter digestibility and crude protein concentration of the grass herbage were higher on the early‐grazing treatment than on the late‐grazing treatment. The cows on the FM treatment had significantly (P < 0·001) higher milk (24·5 kg), solids‐corrected milk (22·5 kg), fat (P < 0·01, 918 g) and protein (831 g) yields than the other three treatments. Cows on the FM treatment had a higher (P < 0·001) DM intake of grass herbage by 2·3 kg DM per cow per day than cows on the AH treatment, which had a DM intake significantly lower than all other treatments (15·2 kg DM per cow per day). The results of the present study showed that grazing in early spring has a positive effect on herbage quality in subsequent grazing rotations. The study also concluded that early spring‐grazed swards stocked at a medium stocking rate (4·5 cows ha^?1; FM) resulted in the highest DM intake of grass herbage and milk production.     

Patterns of leaf and root regrowth, and allocation of water-soluble carbohydrate reserves following defoliation of plants of prairie grass (Bromus willdenowii Kunth.)

This study utilized leaf stage‐based defoliation intervals to describe the concentrations and contents of water‐soluble carbohydrate (WSC) and nitrogen (N) in stubble and root reserves and their effect on the regrowth of prairie grass (Bromus willdenowii Kunth.) plants. The priority sequence for allocation of WSC reserves during the regrowth period was also investigated. There were substantially higher concentrations of WSC and N in the stubble compared with the roots following defoliation, confirming the stubble as the primary site for energy storage, with roots playing a lesser role. However, high R² values for the relationships between WSC concentration in roots and regrowth variables suggested that plants of prairie grass were reliant on WSC reserves from the roots in addition to the stubble to meet the energy requirements of plants until adequate photosynthetic tissue had been produced. The sequence of priority for allocation of WSC reserves followed the order of leaf growth, root growth and tillering during the regrowth period. Although WSC reserves were identified as the primary contributor to plant regrowth following defoliation, there was also a strong relationship between stubble N concentration and regrowth variables.     

Interaction between water-soluble carbohydrate reserves and defoliation severity on the regrowth of perennial ryegrass (Lolium perenne L.)-dominant swards

A field-study was undertaken in Hamilton, New Zealand to determine if there was an interaction between water-soluble carbohydrate (WSC) reserve content and defoliation severity on the regrowth of perennial ryegrass-dominant swards during winter. Perennial ryegrass plants with either low or high WSC content were obtained by varying the defoliation frequency. At the third defoliation at the one-leaf stage and at the first defoliation at the three-leaf stage (harvest H₁), swards were mown with a rotary lawnmower to residual stubble heights of 20, 40 or 60 mm. All swards were then allowed to regrow to the three-leaf stage before again defoliating to their treatment residual stubble heights (H₂). Frequently defoliated plants contained proportionately between 0·37 and 0·48 less WSC in the stubble after defoliation, depending on the severity of defoliation. There was no interaction between WSC content and defoliation severity for herbage regrowth between harvests H₁ and H₂. Herbage regrowth was lower from swards containing low WSC plants compared with high WSC plants (2279 vs. 2007 kg DM ha⁻¹). Furthermore, swards defoliated to 20 or 40 mm had greater herbage regrowth compared with those defoliated to 60 mm (2266, 2249 and 1914 kg DM ha⁻¹ for swards defoliated to residual stubble heights of 20, 40 and 60 mm, respectively). Regrowth of perennial ryegrass was positively correlated with post-defoliation stubble WSC content within defoliation severity treatment, implying that WSC contributed to the defoliation frequency-derived difference in herbage yield. However, the effect of defoliation severity on herbage regrowth was not associated with post-defoliation stubble WSC content.     

The effects of treading by dairy cows on soil properties and herbage production for three white clover‐based grazing systems on a clay loam soil

White clover can reduce fertilizer‐N requirements, improve sward nutritive value and increase environmental sustainability of grazed grasslands. Results of previous experiments in glasshouse conditions and on mown plots have suggested that white clover may be more susceptible than perennial ryegrass to treading damage on wet soils. However, this phenomenon has not been investigated under actual grazing conditions. This experiment examined the effects of treading on clover content, herbage production and soil properties within three clover‐based grazing systems on a wet soil in Ireland for 1 year. Treading resulted in soil compaction, as evidenced by increased soil bulk density (P < 0·001) and reductions in the proportion of large (air‐filled) soil pores (P < 0·001). Treading reduced annual herbage production of both grass and white clover by similar amounts 0·59 and 0·45 t ha^?1 respectively (P < 0·001). Treading reduced the sward clover content in June (P < 0·01) but had no effect on annual clover content, clover stolon mass or clover content at the end of the experiment. Therefore, there was little evidence that white clover is more susceptible to treading damage than perennial ryegrass under grazing conditions on wet soils.     

The response of a perennial ryegrass (Lolium perenne L.) seed crop to nitrogen fertilizer application in the absence of moisture stress

Responses of perennial ryegrass (Lolium perenne L.) to nitrogen (N) fertilizer application rates and timings vary widely, because water is often limiting. Yield response to N fertilizer application during autumn, late‐winter and spring, and the associated efficiency of use of these inputs, was assessed under conditions of non‐limiting soil moisture during two, one‐year lysimeter studies in Canterbury, New Zealand. There were significant (P < 0·05) increases in seed and herbage yields with increasing N fertilizer application. Seed yields differed with year; greatest yields were 300 g m^?2 in 1996 and 450 g m^?2 in 1997. Seed head numbers (r²=0·77), seeds head^?1 (r²=0·92) and herbage yield (r²=0·92) were the major determinants of seed yield in both years. Irrigation required to maintain the soil between 70% and 90% of field capacity was directly related (r²=0·94 and 0·99 in 1996 and 1997 respectively) to increases in herbage yield. Seed yield, seed quality (thousand seed weight and percentage of seed > 1·85 mg), efficiency of water use, efficiency of N fertilizer use and apparent N fertilizer recovery were greatest when N fertilizer was applied at a rate of 50 kg N ha^?1, 50 or 100 kg N ha^?1 and 150 kg N ha^?1 in autumn, late‐winter and spring respectively; further increases in spring N fertilizer stimulated vegetative growth, but not seed yield. As a management strategy, applying N fertilizer to match the N requirements of the crop during the reproductive stage of growth will result in high yields of high quality seed while minimizing environmental impact.     

Herbage production,nutritive value and animal productivity within hardwood silvopasture,open and mixed pasture systems in Appalachia,United States

Demand for livestock food products is projected to increase dramatically through to 2050. Increased livestock production capacity on marginal lands will be critical to meeting this demand. A 5‐year research effort was undertaken to evaluate lamb and sward productivity within open and hardwood silvopasture (SP) systems in Appalachia, USA. Grazing began in mid to late April each year, with the grazing season averaging 141 d. Grazing system treatments during 2002 and 2003 grazing seasons were as follows: 100% open pasture (OP), 67% OP and 33% SP, and 67% OP and 33% SP with delayed SP grazing initiation (OSD). In 2004, a 100% SP (SP) system was added. Animals were rotationally stocked through either 6 (2002–2004) or 7 (2005–2006) paddocks. Open pasture produced greater (P < 0·001) grazing season herbage yield, while all systems generated similar animal performance. Based on summer solstice, herbage production in spring was greater (P < 0·001) than summer, except in 2003. Total non‐structural carbohydrate (TNC) content was greater (P < 0·05) in spring than in summer, except in 2004. Animal performance was superior in spring versus summer (P < 0·001). Animal plasma urea nitrogen (PUN) was lower (P < 0·05) for OP in 2003. When PUN was correlated with nutritive value indicators, the ratio of TNC to crude protein (CP) had the strongest correlation. The strong correlation indicates the need for synchronized ruminal energy and CP availability. Development of silvopasture from existing woodlots has potential to improve whole farm productivity on marginal lands.