Levels of β-carotene and effects of processing on selected fruits and vegetables of the arid zone of India

Some locally available fruits and vegetables of the arid zone of Rajasthanwere analyzed for their -carotene content; the selected fruits and vegetables were: Dansra (Rhus myserensis), Kachri (Cucumis collosus), fruit Kachri (Cucumis collosus), Kair (Capparisdecidua) and Sanghri (Prosopis cineriria). The -carotene contents of the samples were estimated in fresh and various processed states (blanched, dried and roasted). The results showed the heat lability of -carotene. Among the samples, kair was found to be the richest source of -carotene. Variety, too, had an impact on the content of this fat soluble vitamin.     

Biological characteristics of Ustilaginoidae virens and the screening in vitro of the causal pathogen of false smut of rice and fungicides

Green smut bails were collected from diseased rice panicles and surtace sterilized in sodium chloride for 5 minutes. The orange colored internal tissues were placed on PDA medium kept at 25℃ for 5 d to get pure cultures. PDA plates with the pathogen mycelial pieces in diameter of 5mm were incubated under 9 temperature grades ranged from 13 to 35℃.     

Effects of EGF on seed-setting and yield of rice

Intersubspecies hybrid rice have strong heterosis but low seed-setting rate, Researches have showed that most of unfilled and partiallyfilled grains occurred in late-flowering spikelets on the base of a panicle, which usually maintain quiescent state for a period after flowering.     

Effects of environment and variety on content and molecular weight of β-glucan in oats

Oat β-glucan has been shown to have cholesterol, insulin and glucose lowering effects, which are related to increased viscosity of the intestinal contents. Some important factors influencing the viscosity are molecular weight, structure and concentration. To study the effect of variety and environment on β-glucan content and molecular weight of β-glucan, four oat varieties were grown in 11 different environments (location × year combinations) in a field experiment. The β-glucan content varied between 2.3 and 3.2% and the average molecular weight of β-glucan between 1.73 and 2.02 × 10⁶ g mol⁻¹. There was a significant difference between varieties and environments for both β-glucan content and molecular weight of β-glucan. The effect of environment was much greater on molecular weight (71%) than on β-glucan content (42%), while the effect of variety was greater on β-glucan content (23%) than on molecular weight (4%). There was also a positive significant correlation between β-glucan content and molecular weight of β-glucan (p < 0.001, r = 0.46). These results show that β-glucan content is a better target for plant breeding than molecular weight. However, both factors are important for the physiological effects of β-glucan, and selection for high β-glucan content will probably also give higher molecular weight.     

Long-term frozen storage of wheat bread and dough – Effect of time,temperature and fibre on sensory quality,microstructure and state of water

The objective of this study was to determine effect of storage time, storage temperature and addition of fibre on sensory quality, state of water, microstructure and texture of bread and dough.     

Detection of Sensitivity and Resistance Variation of Magnaporthe grisea to Kitazin P,Carbendazim and Tricyclazole

One hundred and twenty-nine isolates of Magnaporthe grisea from Guangdong, Guangxi, Anhui and Jiangsu provinces of China were tested for resistance frequency to kitazin P and carbendazim, respectively by the distinctive concentration method. The resistance frequency of the isolates to kitazin P which had not been used in practice for about ten years was as high as 79.1%, and only one carbendazim-resistant isolate was detected in Gaoyao, Guangdong Province (with a frequency of 0.78%). Meanwhile, the minimum inhibition concentration (MIC) of hyphal melanization was adopted to detect the sensitivity of M. grisea to tricyclazole. There existed several different degrees of sensitivity to tricyclazole in the melanin biosynthesis of M. grisea, but no relationship was found between these MIC values completely inhibiting melanization in hyphae and the EC50 values of tricyclazole against rice blast tested in vivo. After the isolates were induced by chemical taming or UV irradiation in laboratory, kitazin P-resistant and carbendazim-resistant mutants were recovered by both the methods, but none of tricyclazole-resistant mutant was obtained.     

AMMI analysis of interaction of genotype and environment in chalkiness of indica rice

Chalkiness is an unpleasant trait for rice con-sumer. which is known to be controlled geneti-cally and affected by environment during grainmaturing. We used the model of Additive MainEffects and Multiplieative Interaction (AMMI)for interpreting the result of genotype by env-iornment interaction (G×E) in the chalkinessof indica rice varieties.     

Correlations between ultrastructure and photosynthesis in leaves of rice and barnyard-grass

The ultrastructure of the leaves of rice(Oryza sativa)and barnyardgrass(Echinochloa crus-galli)in their milking stage were studied withelectron microscopy.The content of chloro-     

Isolation of bacteria producing chitinase and inhibiting growth of Rhizoctonia solani

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Rheological and Thermal Properties of Potato Starch

<正>Particle size, rheological and thermal properties of potato starch from Yunnan province of China was investigated. The particle size ranges from 0.429-102.3 μm determined by laser light-scatter. The major flow type of 6 w/v% potato starch was shear-thinning fluid even the shear rate up to 800·s-1, and the gel formed by 6 w/v% potato starch fell to weak gel for its little difference between G′ and G′′, high dependence on frequency and low value of G′(Pa). The hardness and cohesiveness of potato starch gel were 31.3 g and 131.9 g·s, respectively. The thermal properties of potato starch were also determined by DSC at the starch∶ water = 3∶ 1. The To, Tp, and △H of potato starch were 62.23℃, 67.31℃, and 2.22 J·g-1.     

Effects of yield and resistances on rice by application of Si-fertilizer

According to the analysis of 654 soil samples collected from 45 counties in Zhejiang Provence, 73.7% of paddy field showed Si deficient. During 1991 and 1993, in Lishi, Longquang, Qingtian and Tonglu Counties, field experiments were conducted to test the effects of basal or additional Si-fertilizer on rice. Si-fertilizer was produced by Nanjing Inorganic Chemical Manufacture, containing 50% water solu     

Effects of 4PU-30 on leaf senescence and degration of protein and nucleic acid in rice

4PU—30[N—phenyl—'N—(2—chloro—4—pyridyl) urea] is a new type of plant growth regulator with cytokinin properties. It has been confirmed to delay rice leaf senescence effectively. In order to elucidate the physiological role of 4PU—30 in delaying senescence, the changes of protein, nucleic acid contents, and the related activities of degradative enzymes were studied. Shanyou 63, an indica hybrid rice was used for this experiment. In the in vitro experiment, two full—developed leaves from the top during heading stage were collected and cut into 5.0cm segments, They were floated on the surface of distilled water containing 0.1mg/14PU—30 and incubated in darkness at 30 C. The leaves floated on distilled water were used as control.It was observed that chlorophyll content in controlled leaves declined rapidly started from the second day and dropped by 93.4% on the 6th day while that in leaves treated with 4PU—30 declined by 41.4% only. During senescence, specific activities of hemoglobin—digesting     

The Development of β-Glucanase and Degradation of β-Glucan in Barley Grown in Scotland and Spain

The barley mutant TR49 and the cultivar from which it was derived, Troubadour, were grown in Scotland and Spain and harvested in 1991 and 1992. The development of β-glucanase together with the breakdown of β-glucan and the reduction in milling energy were followed during malting. While similar amounts of β-glucan were found in samples from both sites, TR49 showed less response to environmental conditions. Grain from Spain showed higher levels of β-glucanase activity, differences being apparent after steeping and 1–2 days of germination. The milling energy of grain was the same, but, during malting, samples from Spain showed a greater reduction in milling energy than those from Scotland. The most extreme environment in the study was the 1991 season at the Spanish site and was the driest and hottest. But even in the cooler, wetter season of 1992, Spanish grown material showed a greater production of β-glucanase than Scottish grown samples. These findings have important implications for plant breeding as well as the growing and trading of malting barley on a European scale.     

Development of Chinese near-isogenic lines of rice and their differentiating ability of pathogenic races of blast fun-gus

To use common differential varietiesin the world, a set of internationaldifferential varieties, including 6 in-dica and 2 japonica rice varieties,were set up in the midddle of 1960s;in 1976, a set of differential varietiescmposed of 9 japonica varieties withknown resistance gene were estab-lished; and later a set ofnear-isogenic lines (NILs), 4 lines     

Temporal and spatial variations of carbon isotope signature reveal substantial contribution of bracts and internode assimilates to grain filling of japonica rice

Carbon isotope composition(δ¹³C)of a plant organ is an inherent signature reflecting its physiological property,and thus is used as an integrative index in crop breeding.It is also a non-intrusive method for quantifying the relative contribution of different source organs to grain filling in cereals.Using the samples collected from two-year field and pot experiments with two nitrogen(N)fertilization treatments,we investigated the temporal and spatial variations of δ¹³C in source organs of leaf,sheath,internode,and bracts,and in sink organ grain.Constitutive nature of δ¹³C was uncovered,with an order of leaf(?27.84‰)13C of the leaf and sheath presented a diminishing trend from the top(flag leaf and its sheath)to the bottom(the last leaf in reverse order and its sheath).No obvious pattern was found for the internode.For temporal variations, δ¹³C of the leaf and sheath had a peak(the most negative)at 10 days after anthesis(DAA),whereas that of the bracts showed a marked increase at the time point of anthesis,implying a transformation from sink to source organ.By comparing the δ¹³C in its natural abundance in the water-soluble fractions of the sheath,internode,and bracts with the δ¹³C in mature grains,the relative contribution of these organs to grain filling was assessed.With reference to the leaf,the internode accounted for as high as 32.64%and 42.56%at 10 DAA and 20 DAA,respectively.Meanwhile,bracts presented a larger contribution than the internode,with superior bracts being higher than inferior bracts.In addition,N topdressing reduced the contribution of the internode and bracts.Our findings clearly proved the actual significance of non-foliar organs of the internode and bracts for rice yield formation,thus extending our basic knowledge of source and sink relations.     

Synthesis of (3S,3′S)- and meso-Stereoisomers of Alloxanthin and Determination of Absolute Configuration of Alloxanthin Isolated from Aquatic Animals

In order to determine the absolute configuration of naturally occurring alloxanthin, a HPLC analytical method for three stereoisomers 1a–c was established by using a chiral column. Two authentic samples, (3S,3′S)- and meso-stereoisomers 1b and 1c, were chemically synthesized according to the method previously developed for (3R,3′R)-alloxanthin (1a). Application of this method to various alloxanthin specimens of aquatic animals demonstrated that those isolated from shellfishes, tunicates, and crucian carp are identical with (3R,3′R)-stereoisomer 1a, and unexpectedly those from lake shrimp, catfish, biwa goby, and biwa trout are mixtures of three stereoisomers of 1a–c.