叶用莴苣离体培养和植株再生

 以叶用莴苣( Lactuca sativa L. ) 4 个栽培品种的子叶和真叶为材料进行诱导芽分化及生根成苗试验, 从中选出较理想的品种和培养基配方, 建立了我国叶用莴苣栽培品种的快速、高频离体植株再生体系。外植体分化频率可达100 % , 平均每外植体分化不定芽5. 6 个。3 d 苗龄的子叶分化效果最佳。从子叶外植体培养至形成完整小植株仅需30～35 d。     

尾穗苋子叶节离体再生体系的建立

以尾穗苋为试验材料,取其无菌苗子叶节为外植体,对苗龄为3d、5d、7d的不同子叶节苗态、切割方式以及激素配比等分化再生因素进行优化筛选。结果表明:不同子叶节苗态及切割方式对不定茅分化影响不大,其中子叶刚平展开的子叶节,采用子叶全留的切割方式有利于不定茅的生长;培养基激素配比对不定茅的诱导影响显著,单独使用6-BA能诱导子叶节产生不定芽,但芽生长较慢且弱小,NAA和6-BA配合使用能促进不定芽的生长和伸长,生长健壮,其中1.0mg/L 6-BA+0.2mg/L NAA组合对不定芽的诱导与生长效果最佳。MS培养基中添加0.2mg/L IBA诱导生根的效果最好,生根率可达97.8%。     

薄皮甜瓜离体再生体系的优化

通过器官发生途径诱导形成不定芽,建立薄皮甜瓜‘IVF05’植株再生体系,探讨不同外植体及不同的激素组合对不定芽再生的影响。结果表明,子叶近胚轴端外植体的不定芽再生率为90.00%,子叶节的再生率为85.00%,子叶远胚轴端外植体的再生率为31.43%,下胚轴的再生率为0,子叶近胚轴端是‘IVF05’不定芽分化的理想的外植体。不定芽诱导中最适宜的培养基为MS+ABA 0.5 mg·L-1+6-BA 1.0 mg·L-1,在此培养基上产生的愈伤较少,能正常分化的不定芽较多,不定芽的生长较快。在MS+6-BA 0.05 mg·L-1的不定芽伸长培养基上,分化的不定芽能够伸长长大。在MS+IAA 0.2 mg·L-1的生根培养基上无根苗容易生根。从外植体培养到获得完整再生植株需50⁶0 d。     

朝天椒子叶离体培养与植株再生体系的建立

以贵州黎平朝天椒地方种为试材,取其无菌苗子叶作外植体,采用植物固体培养基组织培养法,研究了培养基激素配比、无菌苗生理年龄、子叶部位等对朝天椒子叶的离体接种、诱导愈伤、不定芽分化、芽茎伸长、诱导生根、驯化移栽、成苗等离体培养及植株再生阶段的影响。结果表明:朝天椒子叶适宜芽诱导分化的培养基为MS+6-BA 2mg/L+NAA 0.1mg/L,芽分化率达100%;适宜芽伸长的培养基为MS+KT 2mg/L,芽伸长率可达50%;适宜生根的培养基为MS无激素培养基,生根率可达44%。单个外植体平均分化含15~18个不定芽的芽丛(14~16个芽茎),出苗6~7株。从子叶外植体接种至再生苗出瓶需50~55d,再生苗移栽成活率达98%。该研究构建了贵州朝天椒的高效离体培养植株再生体系,为朝天椒的组织快繁和遗传转化奠定了重要的基础。     

薄皮甜瓜离体再生体系的优化

正通过器官发生途径诱导形成不定芽,建立薄皮甜瓜‘IVF05'植株再生体系,探讨不同外植体及不同的激素组合对不定芽再生的影响。结果表明,子叶近胚轴端外植体的不定芽再生率为90.00%,子叶节的再生率为85.00%,子叶远胚轴端外植体的再生率为31.43%,下胚轴的再生率为0,子叶近胚轴端是‘IVF05'不定芽分化的理想的外植体。不定芽诱     

辣椒三系子叶愈伤组织诱导及植株再生

以辣椒雄性不育系、保持系、恢复系子叶为外植体,研究了6-BA、IAA浓度组合及AgNO3对子叶离体再生培养的影响。结果表明:三系之间在愈伤诱导和不定芽分化上均表现一定的差异,不仅最适的愈伤诱导和不定芽分化培养基不同,且分化出不定芽的数量也表现出较大差异,R-1分化出的不定芽数目明显多于A-1、B-1;AgNO3对愈伤组织诱导没有明显的作用,但3mg/L的AgNO3可提高不定芽的分化率;6-BA/IAA比值为10时与比值为4时相比更有利于不定芽的分化;1/2MS+IAA 0.2mg/L+NAA 0.1mg/L是辣椒三系最优的生根培养基配方。     

苜蓿子叶节离体再生体系的建立

以苜蓿子叶节为外植体,以MS为基本培养基,研究了不同浓度激素对苗蓿离体再生体系中子叶节外植体分化、不定芽诱导、不定芽伸长及生根的影响,以期建立高效的苜蓿子叶节离体再生体系.结果表明:苜蓿子叶节再生最佳萌发培养基为MS;最佳诱导培养基为MS+1.0 mg/L 6-BA,诱导时间为7～10 d;不定芽伸长的最佳培养基为MS+0.5 mg/L 6-BA;最佳生根培养基为1/2MS+0.1 mg/L IBA,15 d即可生根.     

不同激素对“五彩珍珠”番茄再生体系的影响

以"五彩珍珠"番茄为试材,选取6d苗龄无菌苗的子叶和下胚轴为外植体,研究了不同激素浓度及配比对番茄愈伤组织的诱导、不定芽分化及生根的影响,以建立番茄再生体系。结果表明:诱导"五彩珍珠"番茄愈伤组织的最佳培养基为MS+0.4mg·L~(-1)IAA+2.0mg·L~(-1)6-BA,其子叶的诱导率为100%,下胚轴的诱导率为95%;不定芽分化的最佳培养基为MS+0.2mg·L~(-1) IAA+(1.0~3.0)mg·L~(-1) 6-BA;诱导生根的最佳培养基为1/2MS+0.10mg·L~(-1)NAA。子叶为最佳外植体,其不定芽的分化率及生长情况均优于下胚轴。     

新疆甜瓜“皇后”再生体系的建立

以厚皮甜瓜"皇后"为试材,研究了6-BA及其与NAA、GA3组合对植株离体再生的影响,以期建立以子叶为外植体的快速高效再生体系。结果表明:甜瓜子叶用MSB+1.0mg/L6-BA诱导分化培养基,不定芽诱导率可达86%以上;培养基中添加NAA不利于"皇后"子叶不定芽的产生;产生的不定芽丛或单芽转至MSB+0.1mg/L 6-BA+0.1mg/L GA3培养基,不定芽均可伸长,且生长状况良好;再生苗转至不添加任何激素的MS培养基生根,生根率达99%。     

桂花子叶切块培养再生苗的研究

以桂花品种大花金桂(Osmanthus fragrans‘Dahuajingui’)的子叶切块为外植体,研究了不同激素水平对桂花子叶切块愈伤组织诱导、芽分化和试管苗生根的影响。结果表明:诱导出的愈伤组织在分化培养基(1/2MS+0.5mg/L TDZ+0.1mg/L NAA)上继代培养9次后分化不定芽,不定芽分化率为22.86%,平均再生苗数为3.22,继代培养10次后,不定芽分化率高达36%。进行生根试验,确定桂花最佳生根培养基为1/2MS+0.5mg/L IBA+2.0mg/L NAA,生根率达到70%。     

Relationship between the intracellular calcium concentration changes and left ventricular hypertrophy and function in the spontaneously hypertensive rats

AIM:To elucidate the relationship between the intracellular calcium concentration changes and left ventricular hypertrophy and function in the spontaneously hypertensive rats (SHR).METHODS:Intracellular free calcium concentrations were measured by Fura 2 methodology and left ventricular function quantitated by cardiac catheterization in 20 SHR aged 10, 22, and 34 weeks and 20 age-matched Wistar-kyoto (WKY) rats.RESULTS:(1) The systolic blood pressure(SBP), intracellular calcium concentrations and left ventricular mass / body weight index (LVM/BW) were significantly higher in all three age groups of SHR than the corresponding groups of WKY; (2) Compared with age-matched WKY groups, the peak left ventricular pressure descending rate(-dp／dt_max) decreased while left ventricular relaxation time constant (τ)increased significantly in SHR aged 22 and 34 weeks. The peak left ventricular pressure ascending rate(dp／dt_max) and the left ventricular contractility index were significantly increased only in the 34 weeks SHR; (3) Intracellular calcium concentrations showed a positive correlation with LVM/BW,SBP,-dp／dt_max and τ(r=0.47-0.83,P<0.01)and a negative correlation with dp／dt_max and the left ventricular contractility index (r=-0.46,P<0.05 and r=-0.81, P<0.01).CONCLUSION:Intracellular calcium overload is one of the potential mechanisms in the induction of left ventricular hypertrophy as well as of systolic and diastolic dysfunction.     

Evaluation of epirubicin cardiotoxity by tissue doppler echocardiography

AIM: To evaluate cardiotoxity effects of epirubicin chemotherapy in lymphoma patients by using conventional and tissue doppler echocardiography.METHODS: Twenty lymphoma patients with epirubicin chemotherapy ［total dose of （450±75） mg/m2］ underwent conventional and tissue doppler echocardiography before every period, twenty healthy volunteers served as control.Conventional echocardiography parameters included left ventricular end-diastolic diameter(LVEDD), left ventricular end-systolic diameter (LVESD)，left ventricular ejection fraction (LVEF)，early and late wave of mitral valve flow (E/A)；Tissue doppler echocardiography parameters included systolic wave (S) and early, late wave of pulse tissue doppler in lateral wall of mitral annulus.RESULTS: Compared to baseline before first period, conventional echocardiography systolic parameters, LVEDD, LVESD, LVEF changed significantly at total dose of 450 mg/m2 (P<0.05)，conventional echocardiography diastolic parameter E/A changed significantly at total dose of 375 mg/m2 (P<0.05)；While tissue Doppler echocardiography systolic parameter S changed significantly at total of dose 300 mg/m2 (P<0.05)，tissue Doppler echocardiography diastolic parameter E/A changed significantly at total of dose 225 mg/m2 (P<0.05 ).Compared to healthy control, there were no significant change every parameters of baseline before first period in twenty lymphoma patients (P>0.05).CONCLUSION: Tissue doppler provides a new sensitive and early method to evaluate LV function in monitoring epirubicin cardiotoxity compared to conventional echocardiography, and diastolic parameter alters earlier than systolic parameter.     

Inhibitory effect of aerobic exercise on left ventricular remodeling and sympathetic neural remodeling in rats with heart failure after myocardial infarction

AIM: To investigate the effects of long-term aerobic exercise on the heart and sympathetic neural remodeling (structure and function remodeling) in heart failure rats induced by myocardial infarction. METHODS: Heart failure model after myocardial infarction was performed by ligating anterior descending coronary artery in the Wistar rats. Four weeks after operation, the rats were randomly divided into sham operation sedentary (S) group, heart failure sedentary (H) group and heart failure exercise (HE) group. The animals in HE group underwent 10-week treadmill running, while those in S group and H group were sustained in a resting state. The cardiac structure and function including left ventricular internal diameter at diastole (LVIDd), left ventricular internal diameter at systole (LVIDs), left ventricular anterior wall diameter at diastole (LVAWDd), left ventricular anterior wall diameter at systole (LVAWDs), left ventricular posterior wall diameter at diastole (LVPWDd) and left ventricular posterior wall diameter at systole (LVPWDs), and cardiac function parameters including fractional shortening (FS) and left ventricular ejection fraction (LVEF) were measured by echocardiography. The myocardium was collected for histopathological observation with Masson staining, and the collagen volume fraction (CVF) was determined. The concentrations of norepinephrine (NE) in the myocardium and plasma were measured by high-pressure liquid chromatography. The frequency domain analysis was applied for determining the heart rate variability (HRV) via subcutaneous recording electrode involving total power (TP), normalized low power (LFn), normalized high power (HFn) and LF/HF ratio. The mRNA expression of collagen type I (Col-I), collagen type III (Col-III), atrial natriuretic factor (ANF), α-myosin heavy chain (α-MHC), β-myosin heavy chain (β-MHC), sarcoplasmic endoplasmic reticulum Ca²⁺-ATPase (SERCA2a) was detected by real-time PCR. The protein levels of nerve growth factor (NGF) and its receptor (TrkA), and tyrosine hydroxylase (TH) were measured by Western blotting. RESULTS: (1) Compared with S group, body weight (BW), LVIDd, FS, LVEF, TP, HFn, the mRNA expression of α-MHC and SERCA2a, and the protein levels of NGF, TrkA and TH decreased (P<0.05). Left ventricular weight (LVW), left ventricular mass index (LVMI), LVAWDd, LVAWDs, LVPWDd, LVPWDs, CVF, plasma and myocardial NE content, LFn, LF/HF, and the mRNA expression of ANF, β-MHC, Col-I and Col-III increased (P<0.05) in H group. (2) Compared with H group, LVW, LVMI, LVIDd, FS, LVEF, TP, HFn, the mRNA expression of α-MHC and SERCA2a, and the protein levels of NGF, TrkA and TH were raised (P<0.05), while CVF, plasma and myocardial NE content, LFn, LF/HF, and the mRNA expression of ANF, β-MHC, Col-I and Col-III decreased (P<0.05) in HE group. CONCLUSION: Long-term aerobic exercise training leads to inhibition of heart and sympathetic neural remodeling and improvement of cardiac function and autonomic modulation in the rats after myocardial infarction.     

Change of heart function and its relationship with AMS during acute high-altitude exposure at 3 658 m in Chongqing soldiers

AIM: To observe the cardiac function during high-altitude exposure in Chongqing soldiers and to discuss its relationship with acute mountain sickness (AMS) by echocardiography. METHODS: The changes of heart function were evaluated during acute high-altitude exposure (3 658 m, 3 d) in 42 healthy young male soldiers by echocardiography. At the same time, the heart rate, blood pressure, blood oxygen saturation, and the incidence of AMS after high-altitude exposure were observed and recorded. RESULTS: Three days after arrival at 3 658 m, the left atrial end-systolic dimension (LADs), left ventricular end-diastolic dimension (LVDd) and right atrial end-systolic dimension were significantly decreased, but the right ventricular outflow tract diameter, pulmonary artery dimension, ejection fraction, cardiac output (CO), pulmonary arterial systolic pressure (PASP) and mean pulmonary arterial pressure were significantly increased compared with the baseline levels in all subjects. The mitral peak E velocity was significantly reduced (P<0.05). A total of 42 healthy young men were recruited and divided into AMS group with 15 subjects and non-AMS group with 27 subjects by Lake Louise scoring after high-altitude exposure. The cardiac function in the plain showed that aortic sinus diameter and LVDd in AMS group were significantly smaller, and PASP was significantly higher than those in non-AMS group. After high-altitude exposure, the LADs in AMS group was significantly smaller than that in non-AMS group (P<0.05). AMS scores and CO in the plain showed significant negative correlation (r=-0.3814, P<0.05). CONCLUSION: Upon acute high-altitude exposure, right ventricular functions of the young male soldiers are damaged with the compensation of the left ventricular functions. Using echocardiography to observe PASP and CO may be helpful for screening the susceptible people of AMS in the plain.     

Mitral annulus velocities and time intervals for evaluation of global left ventricular diastolic function in patients with coronary artery disease

AIM: To detect and compare the longitudinal mitral annulus diastolic velocity and time interval changes by pulsed Doppler tissue imaging (DTI) in patients with angina pectoris (AP) and myocardial infarction (MI), and to explore the value of mitral annulus diastolic velocities and time intervals for evaluation of global left ventricular diastolic dysfunction. METHODS: Fifty patients with established coronary artery disease were divided into AP group (16 cases) and MI group (34 cases). Sixteen age-matched healthy individuals served as the control group. The septum, lateral, anterior and inferior walls of the mitral annulus were displayed, and selected for DTI spectrum sampling. Peak early and late diastolic velocities and their ratio, time to the onset and peak of the early diastolic wave, and regional isovolumic relaxation time were measured, and the average values of the four mitral annular sites were calculated and presented as Em, Am, Em/Am, QEm, TEm and IVRTm, respectively. RESULTS: Compared with the control group, Em and Em/Am were significantly lower in both the AP and the MI groups (P<0.01). Em was even lower in the MI group than that in the AP group (P<0.01). QEm, TEm and IVRTm were significantly longer in the AP and the MI groups than those in control group (P<0.01 or P<0.05). IVRTm was even longer in the MI group than that in AP group (P<0.01). IVRTm had significantly negative correlation with Em (r=-0.64, P<0.01). CONCLUSION: Em, Em/Am, QEm, TEm and IVRTm as measured by pulsed DTI may be promising indexes for quantitative assessment of global left ventricular diastolic dysfunction in patients with coronary artery disease. Em and IVRTm may indicate the severity of ischemic myocardial damage.     

Effects of fluvastatin on the left ventricular function,MHC gene expression and collagen remodeling after myocardial infarction

AIM: To observe the effects of fluvastatin (FV) on the left ventricular (LV) function, MHC mRNA and collagen remodeling of non-infarcted area after acute myocardial infarction (AMI). METHODS: Six hours after ligating left coronary artery, survivors of AMI female SD rats were randomly assigned to: ①AMI control; ②FV; ③sham-operated groups. After 8 weeks of therapy, the LV function, hemodynamics, expression of non-infarcted myocardial MHC mRNA, collagen volume fraction (CVF) and the ratio of type Ⅰ/Ⅲ collagen of non-infarcted area were assessed. RESULTS: Compared with sham-operated group, E wave, E wave deceleration, E/A ratio, LV end diastolic pressure (LVEDP), β MHC mRNA, CVF and the ratio of type Ⅰ/Ⅲ collagen were all significantly increased in AMI group, while fractional shortening (FS), ejection fraction (EF), mean arterial pressure (MAP) and α MHC mRNA were all significantly decreased. In comparison with AMI group, E wave, E wave deceleration, E/A, LVEDP, β MHC mRNA, CVF and the ratio of type Ⅰ/Ⅲ collagen were all significantly decreased, while FS, EF, MAP and α MHC mRNA were all significantly increased in FV group. CONCLUSION: FV improves the LV function after AMI and has beneficial effects on reversing LV myocardial pathologic switching of MHC isoform and collagen remodeling.     

Xinshuaikang inhibits myocardial autophagy and MAPK/ERK1/2 signaling pathway in rats with chronic heart failure

AIM:To explore the effect of Xinshuaikang on myocardial autophagy in the rats with chronic heart failure and its relationship with the MAPK/ERK1/2 signaling pathway. METHODS:The rats were divided into sham group, model group (rat model of chronic heart failure was established by ligation of anterior descending branch of left coronary artery), low-, middle-, and high-dose Xinshuaikang treatment (TL, TM and TH) groups and captopril group (treated with captopril as positive control), with 12 in each group. Doppler echocardiography was used to evaluate the cardiac function. The morphological changes of the myocardium were observed by HE staining. TUNEL staining was used to detect cardiomyocyte apoptosis. The expression of microtubule-associated protein 1 light chain 3-Ⅱ (LC3-Ⅱ) in the myocardium was detected by immunofluorescence labeling. The protein levels of p-ERK, p-p38 MAPK, LC3-Ⅱ, beclin-1 and p62 in the myocardium were determined by Western blot. RESULTS:Compared with sham group, left ventricular end-diastolic dia-meter (LVEDD) and left ventricular end-systolic diameter (LVESD) in model group were increased, while left ventricular posterior wall thickness at end-diastole (LVPWTd), left ventricular posterior wall thickness at end-systole (LVPWTs), left ventricular ejection fraction (LVEF), cardiac output (CO), left ventricular diastolic pressure (LVDP), left ventricular systolic pressure (LVSP) and maximum rate of rise/decrease of left ventricular pressure (+dp/dt_max/-dp/dt_max) were decreased (P<0.05). The myocardial cells were deformed and necrotic, and the myocardial fibers were broken, with inflammatory cell infiltration. The apoptotic rate, the positive rate of LC3-Ⅱ, and the protein levels of p-ERK, p-p38 MAPK, LC3-Ⅱ/LC3-I and beclin-1 were increased, and the protein expression of p62 was decreased (P<0.05). Compared with model group, the levels of LVEDD and LVESD were decreased, LVPWTd, LVPWTs, LVEF, CO, LVSP, LVDP, +dp/dt_max and -dp/dt_max were increased in Xinshuaikang groups and captopril group (P<0.05). The morphological changes of myocardial cells were gradually returned to normal, and inflammatory cell infiltration, the apoptotic rate and the positive rate of LC3-Ⅱ were decreased. The protein levels of p-ERK, p-p38 MAPK, LC3-Ⅱ/LC3-I and beclin-1 were decreased, and the protein expression of p62 was increased (P<0.05). CONCLUSION:Xinshuaikang inhibits myocardial auto-phagy to play a role of cardiac protection in the rats with chronic heart failure, and its mechanism may be related to inhibition of MAPK/ERK1/2 signaling pathway.     

Evaluation of left ventricular diastolic dysfunction at the early stage of pressure overload-induced myocardial remodeling in rats

AIM: To investigate the evaluation method of left ventricular diastolic function and myocardial hypertrophy induced by pressure overload in rats.METHODS: Male Sprague-Dawley rats were subject to left ventricular pressure overload by transverse aortic constriction (TAC).Cardiac structure and diastolic function were evaluated by echocardiography, hemodynamic analysis and examination of hydroxyproline concentration in the myocardial tissues.RESULTS: Compared with the sham-operated controls, left ventricular wall dimension in diastole significantly increased in the rats 3 weeks after TAC .Left ventricular early diastolic posterior wall motion velocity (E') significantly decreased in the rats 3 weeks after TAC , and was much lower than that in the rats 6 weeks after TAC.Left ventricular mass to tibia length in TAC rats was much higher than that in sham-operated controls .The ratio of maximum rate of degression of left ventricular pressure (dp/dt_min) to left ventricular systolic pressure (dp/dt_min/LVSP) started to decrease in TAC rats in the 3rd week (48.9±5.9 vs 63.5±9.9) and significantly decreased in TAC rats in the 6th week as compared with sham-operated controls (35.4±4.0 vs 54.4±2.9, P<0.01).Sirius red-stained collagen in cardiac interstitium, especially around the blood vessels, was increased in TAC rats.Six weeks after TAC, a significant increase in the content of myocardial hydroxyproline was observed.CONCLUSION: The early diastolic posterior wall motion velocity (E') detected by tissue Doppler imaging is a sensitive indicator of diastolic dysfunction at the early stage of myocardial remodeling induced by pressure overload in rats.     

Correlation between orthostatic hypotension and left ventricular structure and function in elderly patients

AIM:To study the prevalence rate of orthostatic hypotension (OH) in elderly patients and to investigate the correlation between OH and left ventricular structure and function. METHODS:This study was conducted in 126 hospitalized patients aged ≥80 years in our department. The orthostatic blood pressure was measured in the supine position after resting for over 5 min and at 0 and 2 min after standing. The patients were divided into non-OH group (the patients without OH, n=81) and OH group (the patients with OH, n=45). Cardiac ultrasonography was conducted in all patients, and the parameters of the left ventricular structure and function were measured and calculated. The correlation between OH and left ventricular structure and function was also analyzed. RESULTS:The prevalence of OH was 35.7％ in elderly patients. The significant differences between non-OH group and OH group for left ventricular mass index (P<005), left ventricular end-diastolic diameter(P<0.05), left ventricular end-systolic diameter (P<0.05), ejection fraction (P<0.01) and fractional shortening (P<0.01) were observed. Correlation analysis revealed that OH was positively correlated with the parameters of left ventricular structure, and was negatively correlated with the parameters of left ventricular function. CONCLUSION: Elderly patients are at high risk of OH. OH patients have worse left ventricular structure and function as compared with non-OH patients.     

Effects of irbesartan and perindopril on pressure overload-induced cardiac hypertrophy in rats

AIM: To observe the effects of irbesartan and perindopril on pressure-overload cardiac hypertrophy in rats. METHODS: 40 male adult Sprague Dawley rats were divided into 5 groups. One was sham operation group, other four were aortic banding groups. One week after operation, all rats were gavaged with normal saline, perindopril, irbesartan or combination of perindopril and irbesartan. Morphometric determination, calcineurin (CaN) expression, CaN and sarcoplasmic reticulum (SR) Ca²⁺-ATPase activity were performed at the end of 6 weeks of drug intervention. RESULTS: Left ventricular mass index (LVMI), transverse diameter of myocardical cell (TDM), CaN activity were remarkably decreased after drug intervention and this decrease was most remarkable in the combination group. SR Ca²⁺-ATPase activity increased after drug intervention, especially in the combination group. CaN expression in myocardium were remarkably decreased after drug intervention. LVMI was positively correlated with TDM and CaN, negatively correlated with SR Ca²⁺-ATPase. CONCLUSION: Both irbesartan and perindopril decrease CaN activity, increase SR Ca²⁺-ATPase activity and combination of them has synergic effects on regressing of ventricular hypertrophy.