天牛消化纤维素的机制研究

天牛能否自身消化植物的纤维素,是长期来争论未决的问题,本课题用聚丙烯酰胺凝胶等电聚焦电泳方法着重研究了纤维素酶复合酶中C_X酶在虫体中的来源。对天牛科18种雌成虫、16种雄成虫、12种幼虫消化道C_X酶进行了电泳分离,并根据C_X酶同工酶在不同虫态与不同发育程度间的差异,探讨了天牛科C_X酶的来源,结果证明天牛中肠肠壁细胞能自身分泌C_X酶。     

卵巢线虫(Contortylenchus genitalicola)在中国松褐天牛上的分布与作用研究

[目的]日本学者Kosaka与Ogura发现松褐天牛成虫除携带松材线虫外,雌成虫卵巢内还携带有另一种线虫,他们将其命名为卵巢线虫,并认为这种线虫是松褐天牛成虫的寄生性线虫。我国对该种线虫的研究迄今未见报道。为了证明这种线虫在我国是否存在和分布,开展了本项研究。[方法]分期分批捕获刚羽化的松褐天牛雌雄成虫,采用解剖松褐天牛成虫松树木质部及感病的松褐天牛幼虫等方法,调查卵巢线虫的存在与分布。[结果]通过调查研究,发现我国的松褐天牛成虫体内有该卵巢线虫存在,分布于松褐天牛成虫、幼虫体内和松树木质部3个部位;在松褐天牛成虫体内的卵巢线虫通过松褐天牛雌成虫产卵而接种、进入寄主树木木质部中。同时,木质部的卵巢线虫也有一部分进入松褐天牛幼虫体内寄生,另一部分仍在木质部生活,当松褐天牛幼虫再次发育为成虫时,在木质部中的卵巢线虫和已被寄生的松褐天牛幼虫体内的卵巢线虫再次进入松褐天牛成虫体内,完成循环。卵巢线虫在松褐天牛雌、雄虫体内均有分布,携带率为44.4%,其中松褐天牛雌成虫携带率为43.8%,松褐天牛雄成虫携带率为45.0%,两者间无显著差异;每头松褐天牛成虫平均携带卵巢线虫574条,其中雌成虫平均携带816条,雄成虫平均携带308条,具显著差异。初步研究表明,卵巢线虫在松褐天牛成虫体内只能完成产卵到1~4龄的幼虫阶段,不能完成一个完整的世代;在松褐天牛幼虫体内寄生和在木质部生活的卵巢线虫能完成一个完整世代,但具体过程尚不清楚;调查中未观察到卵巢线虫对松褐天牛成虫有寄生致病或致死的现象,但对松褐天牛幼虫有寄生致死的能力;卵巢线虫常与松材线虫同时存在,而且侵入松树及离开松树的方式与松材线虫相同。[结论]我国松褐天牛体内也发现有卵巢线虫存在;目前尚不能证明该线虫对松褐天牛成虫具有寄生致死性,但对松褐天牛幼虫具有一定的寄生致死性;卵巢线虫的生活史与松材线虫相似,是否与松材线虫一样对松树具有危害性以及其病理作用还有待于进一步研究。     

韩国原麝分布区50年间的变迁

在南朝鲜于1999年的2月至12月间,根据有关文献,调查问卷和对原麝的种类,粪便,及其在有雪和无雪的季节里的野外踪迹的调查,作者概述了50年中原麝的数量和分布区的变化。结果表明:从50年代至1999间,原麝(Moschus moschiferus parvipes)的分布区呈明显缩减之势。由于在原麝分布区中大规模地进行公路及林道的建设,导致了其生境片断化和丢失。而且牟取利润的非法猎捕也是造成原麝的种群数量和分布区缩减的主要原因,在高海拔地区的林道也常被盗猎者所利用。为保护韩国原麝资源,急待严格禁止非法猎捕行为。原麝生活习性方面的研究,为合理的保护和管理原麝提供了重要的基础信息。图1参9。     

The distribution patterns and temporal dynamics of carabid beetles (Coleoptera:Carabidae) in the forests of Jiaohe,Jilin Province,China

Carabid beetles,predatory insects,are abundant in forests and sensitive to environmental changes.The dis-tribution patterns and diversity of carabid beetles in several natural forests were studied to provide a basis for evaluating the importance of a forest in the protection of carabid beetle diversity.Carabids were captured by pitfall traps during their seasonal activity from 2012 to 2013 in a poplar-birch forest,ash-walnut forest and broad-leaved Korean pine forest.A total of 5252 individuals,representing 21 species,were col-lected.Carabid abundance was highest in the broad-leaved Korean pine forest and lowest in the ash-walnut forest.Carabus billbergi Mannerheim and Pterostichus pertinax(Tschitscherine) were the dominant beetle species in each stand.Carabus canaliculatus Adams was dominant in the poplar-birch and ash-walnut forests,and Leistus niger Gebler was dominant in the ash-walnut forest.The carabids were affected differently by stand factors.C.billbergi and P.per-tinax was positively correlated with mean DBH.C.cana-liculatus and L.niger were not positively correlated with any stand factors.The broad-leaved Korean pine forest with greater age,large DBH and thick leaf litter fostered a high diversity of carabid species.The main yearly activity period for most carabids was during July.Different carabid species responded differently to seasonality,and the activity period of several species was relatively late (August) in the year.     

Boring activity on coniferous tree branches byXylotrechus villioni (Villard) larvae (Coleoptera: Cerambycidae)

In anAbies homolepis Sieb. et Zucc. forest at Matsumoto, Nagano Pref., cumulative damage byXylotrechus villioni (Villard) (Coleoptera: Cerambycidae) was observed on tree branches. Of the 14 major branches in one sample tree, six had cumulative damage by boring larvae, of which 3 had been bored by multiple larvae. No adult emergence holes were found on the whole tree, suggesting very low survivorship from boring larva to emerging adult. Galleries made by single larvae were often observed to be terminated by a depression apparently carved by a picid woodpecker, suggesting that they are important predators of the cerambycid. The manner of cicatrix formation with phloem tissue over the larval gallery in anAbies veitchii Lindl. branch at Mt. Fuji, Yamanashi Pref. is presented. Studies onXylotrechus villioni (Villard) (Coleoptera: Cerambycidae), a primary borer of coniferous trees in Japan (III). This work was presented at the 104th Annual Meeting of Japanese Forestry Society at Morioka (April, 1993) and 20th International Congress of Entomology at Firenze (August, 1996). This work is supported by a Grant-in-Aid for Scientific Research (No.02660163) from the Ministry of Education, Science, Sports, and Culture of Japan.     

<Emphasis Type="Italic">Bursaphelenchus mucronatus</Emphasis> (Nematoda: Aphelenchoididae) vectored by <Emphasis Type="Italic">Monochamus </Emphasis><Emphasis Type="Italic">urussovi</Emphasis> (Coleoptera: Cerambycidae) in Hokkaido,Japan

Bursaphelenchus mucronatus Mamiya et Enda has been recovered for the first time from adults of the cerambycid beetle, Monochamus urussovi (Fischer), in Hokkaido, Japan. The nematode was also recovered from the inner bark of Picea jezoensis (Siebold et Zuccarini) Carrière and Abies sachalinensis (Fr Schmidt) Masters infested with M. urussovi larvae. PCR–RFLP analysis indicated that B. mucronatus in Hokkaido is the European type.     

RESEARCH ON REGENERATION APPROACHES OF KOREAN PINE FORESTS

Broad-lcavedKoreanpincforcstisthcclimaticclimaxconinunitVofnortheastmoun-tainousregionsofChina.AsthemaintreesPecies,Koreanpinehasexccllentwood.AgreatattentionbasbccnpaidtorecoveringtheseforesttyPesafterharvesting.Additional1y,KoreanpineisoneofkeyregcncrationtreesPeCiesinthisregion.TherehasbeenlargeareaofKorcanpincplantations.Howevcr,thercaresomeproblemsncedingtobestUdicdfwtheramongwhichoneistoselectsuitabIeropenerationapproacl1esofKoreanpincforesls.WHATISTHEPROBLEM?ThcnatUralregen…     

Review of the Eilema group of lichen moths from South Korea,with description of one new species(Lepidoptera:Erebidae:Arctiinae:Lithosiini)

This review details the Korean species of the Eilema group in the subfamily Arctiinae of Erebidae: 19 species in five genera(Manulea, Collita, Katha, Dolgoma,and Wittia), a new species(Collita hwacheonensis Bayarsaikhan Bae, sp. nov.), and an unrecorded species [C.vetusta aegrota(Butler 1877)]. All species are diagnosed,and figures of the adults and genitalia and a key to species based on male genital structure of Eilema group in South Korea are included.     

New parasitoids of<Emphasis Type="Italic"> Saperda populnea</Emphasis> (L.) (Col. Cerambycidae) in Bulgaria

The parasitoid complex of Saperda populnea (L.) (Col., Cerambycidae) was studied during the period 2002–2003 at different sites in Bulgaria. Galls containing cerambycid larvae were collected on branches of aspen (Populus tremula L.), goat willow (Salix caprea L.) and hybrid poplars and were studied under laboratory conditions. As a result, four new species for the parasitoid complex of S. populnea in Bulgaria were recorded: Dolichomitus tuberculatus (Geoffroy), Xylophrurus lancifer (Gravenhorst), Gelis ornatulus (Thomson) (Hym., Ichneumonidae) and Dendrocerus serricornis (Boheman) (Hym., Megaspilidae). D. serricornis and G. ornatulus are new species for Bulgarian fauna. D. tuberculatus and X. lancifer were found as primary parasitoids, and G. ornatulus and D. serricornis as hyperparasitoids. The primary parasitoids developed solitarily in the host larvae. D. serricornis was reared gregariously from a puparium of Billaea irrorata (Meig.) (Dipt., Tachinidae). The host of G. ornatulus was not identified, but the hyperparasitid could be connected with Iphiaulax impostor (Scop.) (Hym., Braconidae). The mortality of S. populnea caused by the parasitoids was 2.0–6.3%.     

Distribution and Host Plants of Asemini in China and Potential Invasive Threat of Alien Species

Asemini is a Northern Hemisphere cerambycid tribe,and an important forest pest, mainly causing damage to coniferous plants.More important,as one kind of quarantine insects,Asemini can bring about many troubles in timber trade, therefore,the alien species should be paid more attentions and need to be quarantined in order to prevent them to be introduced to China.In China, there are 4 genus 26 species of Asemini.Host plants of Asemini include 15 genus 36 species of 7 families, and the main host plants are in Pinus,accounting for 30.56%of the total.A fauna analysis indicated that 15 species of Asemini distributed in the Palareartic Realm,accounting for 57.69%of the total. The species predominated in China,probably because gymnosperms were the main vegetable type in this region.This paper lists all the known species and genera of Asemini and their host plants in China,and discusses their distribution.The aim is to arouse the concerns of these insects and provide a reference for the relevant work.     

中国幽天牛族昆虫分布、寄主分析及国外种类潜在入侵威胁

幽天牛族是一类重要的林业害虫,我国已记载昆虫4属26种.幽天牛族昆虫的寄主多为针叶类裸子植物,已记载的寄主植物有7科15属36种,其中以松科松属的种类最多,占所有记录到种的寄主植物的30.56％,而且危害松属的幽天牛数量也最多.对我国目前分布的26种幽天牛族昆虫进行区系分析,发现幽天牛族昆虫以古北区成分为主,这可能与古北区针叶植物较多有一定关系.我国的地理环境及生态植被,均可以满足幽天牛族非中国种的分布及寄主需求,非常适宜幽天牛族非中国种的生存和繁衍,因此应该提高警惕,严防国外种类传入我国,以保护我国树木安全.     

Genetic Variation and Population Structure in Korean Endemic Species: V. Corylopsis coreana (Hamameliaceae)

The allozyme diversity of ten Korean populations of Corylopsis coreana Nakai, a shrub endemic to Korea, were examined. Although Korean populations are isolated, they maintain moderate levels of allozyme variation (average percent of loci polymorphic=22.4%, mean number of alleles per polymorphic locus=3.97, mean expected heterozygosity=0.109) compared to the means of 26 woody endemic species. A high level of heterozygote deficiency was observed in populations of C. coreana (mean F _IS=0.232). Significant heterogeneity in allele frequencies were detected between populations at all polymorphic loci (p<0.001), among-population genetic differentiation accounted for 12.3% of the total variation. Indirect estimates of the number of migrants per generation (Nm) (1.77, calculated from G _ST) indicate that gene flow is moderate among populations of C. coreana. The allozyme diversity and population structure in C. coreana may be attributed to relatively long-distance flight of pollinators in early spring, gravity seed dispersal, restricted distribution, and high density in the standing populations of the species.     

NITROGEN NUTRIENT MECHANISM IN SECONDARY SUCCESSION PROCESS OF THE MIXED BROAD-LEAVED/ KOREAN PINE (PINUS KORAIENSIS) FOREST

INTRODUCTlONThemixcdbroad-leaved/Pinuskor`IiensiSforestwhichisoneofthemainforestsofNortheastChina,isintlleCllangbaiMountail1s(JilinProvince)a11dtheXiaoxing'anMotu1-tains(inHeilonroIangProvince).Thisistl1empicalmixcddeciduous/co11iferforestvegcta-tionofthcarca.But,duetocuttingandanunbalancebctWeenharvesti11gandanntlalin-crement,themixedbroad-leaved/PinuSko-raiensIjforestsha1,ebeenreplacedwithsec-ondaryforests(includingthelargearPaofsecondaryforestleftfrol11thepast)sinceltsclearcutti…     

Nitrogen nutrient mechanism in secondary succession process of the mixed broad-leaved/Korean pine (Pinus koraiensis) forest

Chemical and biochemical analysis methods were used to monitor the variations of nitrogen nutrient among the dominance trees species in secondary succession process of the mixed broad -leaved/Korean pine forest on Changbai Mountains, Northeast China. Amounts of total nitrogen, ammonium and NRA in soils of virgin broad-leaved/Korean pine forest which is in climax were higher than those ofsecondary birch forests those are in succession stage. The amount of nitrate was in the other hand. In climax, dominance trees species are tolerant mesophytic trees such asPinus Koraiensis, Tilia amurensis, Acer mono and alsoFraxinus mandshurica, they are all ammonium + nitrate adapted species, but they show a preference for the ammonium rather than those of the pioneer trees species in secondary birch forest, such asPopulus davidiava andBetula platyphylla. Because they have more ammonium in their leaves and roots, especiallyPinus koraiensis. Populus davidvana andBetula platyphlla are intolerant trees, amounts of nitrate and total nitrogen is higher in their leaves and roots and also NRA in their leaves, so they preference for the nitrate rather than the others. In secondary birch forest, the regeneration trees species adapt their nitrogen nutrient to the variation of nitrogen nutrient situation in soil, finally they could survival well and the secondary birch forest would succession to climax. In climax, dominance trees species adapt their Nitrogen nutrient to the situation in soil and there are not strong competition in nitrogen nutrient among them, so they can coexist well and keep the climax as stable vegetation.     

长白山红松阔叶林及其次生林草本植物多样性季节动态研究

研究了原始红松阔叶林及其次生林草本植物物种组成及多样性动态,共记录了179种草本植物,其中红松阔叶林125种,次生林160种。红松阔叶林及其次生林草本植物的月季动态变化明显,物种数、Simpson多样性指数、Shannon-wiener指数及均匀度指数均表现为先增加再减小。不同月份次生林内草本物种数均明显高于红松阔叶林。红松阔叶林下草本植物垂直方向呈现明显的分层现象;在整个生长季内,次生林下草本植物高度均大于红松阔叶林。     

在韩国片断化林区内道路对小型啮齿类种群的影响

The road effect on small rodent population is investigated at 8 fragmented forest areas in the Baekdudaegan moun-tain range, South Korea in September 2001, We especially focused on the distribution and body condition of small rodents near the roads, Korean field mouse (Apodemus peninsulae) seems to be more sensitive to the existence of a road than striped field mouse (Apodemus agradus), Korean field mouse prefers interior forest area to around road. Striped field mouse is a habitat generalist and has wide distributional range around road, but Korean field mouse is forest-inhabiting species and their distribu-tion is limited in forest area. These results suggest the effect of road is different on each small rodent species and their habitat preferences.     

Artificial regeneration of Korean spruce in northeast China

Korean spruce (Picea koraiensis Nakai) has been included as a Chinese regeneration species since the 1960′s. In the 1970′s it became one of the four most important species in the northeast forest region of China. Korean spruce is a timber species with a wide range of adaptation, high survival rate, and few diseases or pests. Studies of 30-year trials show that the regeneration of korean spruce is successful and has a prospective future.     

Life History Process and Conservation of Korean Pine Populations in the Xiaoxing''''an Mountains of Northeast ChinaThis research was supported by the UNDP Project CPR/96/109

分析了中国东北小兴安岭林区红松种群的生活史过程及其影响因素,对红松生活史的４个阶段进行了分析,并讨论了其进化和保护对策．红松的传粉和受精主要受天气条件的影响．林冠下的光照是控制红松幼苗生长和存活的主要因素．红松种子生产的周期性波动会引起有关动物种群的相应变化．动物是阔叶红松林生态系统中不可缺少的组成成分,松鼠在红松种群的天然更新中起着非常重要的作用．了解森林生态系统中物种的协同进化关系对于我们保护森林生物多样性和生态系统的完整性有着重要的意义．在红松的保护中,我们不仅要保护树木本身,而且还应当保护与红松相关的动物、生境乃至整个生态系统．     

基于“近自然林业”的红松林主要树种重要值的研究

以“近自然林业”为指导思想,对凉水自然保护区不同类型红松林主要树种的重要值进行了研究。结果表明：红松在天然林内并不占据优势地位;红松人工纯林内红松的重要值都很大,都大于同林分内的阔叶树主要树种的重要值之和;林隙透光抚育林内红松的重要值为107．98％,而其他几个阔叶树种色木、白桦、椴树和杨树的重要值相加为121．69％,主要树种中针叶树种和阔叶树种的比值接近1∶1,这种重要值的比率使整个林分的针阔比处于一种平衡的状态。     

Study on community structure and self-sustaining mechanism of broad-leaved/Korean pine forests in northeast China

INTRODUCTloNbePthemp`vkoItw)isanli11pe1tclhabepeicinnoed1castO1ina.Thcspeic~rminn1bo\vithInanyhar~~,inbotScalledndbroadileated/KOrCantwthisisthecham~honin~toinla.bendboare~lycalledKrsPincbofOrdnd.ThebohavcboexPloitalforwtahalf~,withiISareabeing~boIhan5oto5~inthiswil,].TO-tw-oroldgrowthbonaatonlyiminfewedwr.beusePaSttimeduetOwithe~mCChanisl1isofthenabotwpePlanboonsofKrsptheunmaseeqclear~ofhsenahahenrstaredanopmcaedtyforndnd'l,.ndmamAnboontisetOPofnd3l.Hot`toodthehighqtaltyorboPli…