翻白叶树田间接种VA菌根菌效果

在田间自然条件下(非灭菌条件下)应用VA菌根菌对翻白叶树幼苗进行人工接种,接种苗木3个月后,接种苗生长速度明显大于对照苗,3个月时高于对照40.1%,至6个月时接种苗高生长高于对照苗的59.1%,9个月时高于对照53.7%;对苗木根系进行镜检,发现接种苗与对照苗均有VA菌根菌感染,接种苗感染率为100%,对照苗感染率为10%,接种苗的VA菌根菌感染程度远大于对照苗。试验证明,在田间自然条件下对翻白叶树幼苗接种VA菌根菌,能显著促进其高生长。     

联合固氮菌对桉树青枯病菌的抑制作用研究

采用纸碟法和液体比浊法研究了联合固氮菌对桉树青枯病菌茄拉尔氏菌(Ralstonia solanacearum (E.F.Smith) Yabuuchi)的抑制作用.结果表明两株联合固氮菌1和固氮菌2及其培养物上清液对青枯菌Ra均有不同程度的抑制作用,上清液抑制效果好于菌悬液抑制效果,混合菌株的抑制作用高于单一菌株的抑制作用.温室接种结果表明,接种固氮菌1和同时接种固氮菌1及青枯菌Ra的处理比不接种对照(CK)桉树苗高增加33.19%和31.09%;单接种固氮菌1的桉树苗地上干质量比对照(CK)增加123.23%,同时接种固氮菌1及青枯菌Ra的桉树苗比对照(CK)地上干质量增加93.22%;接种固氮菌1和同时接种固氮菌1及青枯菌Ra的处理比对照(CK)桉树苗地下干质量分别增加101.52%和71.81%.接种固氮菌可以降低桉树苗木死亡率38.24%.桉树幼苗温室接种试验证明接种固氮菌的桉树苗木生长旺盛,茎干较对照粗壮,长势整齐,固氮菌可以提高桉树苗木对青枯病的抗性.     

接种内生菌根菌对尖叶杜英高生长的影响

在不同栽培模式下应用内生菌根菌接种尖叶杜英幼苗试验证明,接种苗木高生长均明显高于对照。其中,营养袋苗接种高生长速度比对照高26.85%;盆苗接种比对照高8.14%;地栽苗接种苗比对照高12.88%。     

相思根瘤菌耐酸性研究及耐酸菌株的筛选

对２２株相有瘤菌耐酸性的比较结果得出：大多数的相思根瘤菌不耐酸。在ｐＨ５．０条件下大多数根瘤菌生长不好，只有ＩＳ００４、ＩＳ００２、ＣＲ００２和ＬＲ００７菌株生长较好，在培养基ｐＨ３．８６条件下和在ｐＨ４．９土壤悬液中，ＩＳ００４和ＩＳ００２菌株的生长及存活率较其它菌株高，通过在酸性条件下生长与结瘤的试验，初步筛选出耐酸菌株ＩＳ００２。     

喜树育苗和接种菌根菌试验

用苏格兰球囊菌 [Glomuscaledonium (Nicol&Gerd .)Trappe&Gerd .]和地表球囊 [Glomusversiforme (Karsten)Berch]菌种接种喜树幼苗。试验结果表明 :喜树幼苗根均可与两种菌种菌形成菌根 ,接种前者的苗木菌根感染率为 5 3% ,接种后者的苗木菌根感染率为 4 0 % ,非接种苗木 (对照 )的感染率 6 .7%。接种菌根菌后的苗木总生物量、根茎比及根系长度和地径有所提高 ,反映了 2个菌种均促进了喜树幼苗的生长 ,但对幼苗高生长的促进作用不大。     

根瘤菌、菌根菌对厚荚相思苗期生长的影响

对厚荚相思苗期进行接种根瘤菌、菌根菌、根瘤菌＋菌根菌的比较试验,结果表明：接种菌根菌的促生效果显著。幼苗高生长、地上部分干重、地下部分干重、根瘤个数、根瘤重量平均数比对照分别提高了53．36％。73．30％,89．69％,224．01％和186．96％。接种各菌种对幼苗的促生效果排序为：菌根菌＋根瘤菌〉根瘤菌〉菌根菌〉对照。苗木根部菌根菌感染强度排序为：菌根菌〉菌根菌＋根瘤菌〉对照。     

接种内生菌根菌对尖叶杜英高生长的影响

在不同栽培模式下(营养袋苗、盆苗、地栽苗)应用内生菌根菌对尖叶杜英幼苗进行人工接种,接种苗木高生长均明显高于未接种苗.其中营养袋苗接种苗生长速度明显大于对照苗,比对照快了26.85%;盆苗接种苗高生长量高于对照苗8.14%;地栽苗接种苗高生长量高于对照12.88%.试验证明,在幼苗期对尖叶杜英接种内生茵根茵,能显著促进其高生长.     

木麻黄弗兰克氏菌接种技术与接种效果的研究

本文对木麻黄接种弗兰克氏菌的时间、方法、接种剂、接种量、菌株混合接种施肥与接种效果进行了研究。结果得出：最达接种时间为纪苗期；接种方法为切根接种法：海藻酸钙胶囊干、湿菌剂、草炭、蛭石菌与液体接种剂这五处接种剂之间的接种效果没有显著差异，接种量为高接种量较好，弗兰克氏菌与芽孢杆菌混合接种的效果和弗兰克氏菌单接种的效果好；施营养元素Ｐ、Ｍｇ、Ｃａ、Ｃｏ和在红壤中加石灰能促进木麻黄的生长与结瘤；接种弗寺     

红菇和正红菇菌种接种三个乡土树种的苗期效果

利用正红菇和鳞盖红菇2个菌种，对木荷、红锥和黎蒴3个乡土树种的苗木进行接种试验，定期观测苗木的苗高、地径及最终生物质量。结果表明两个菌种对3个乡土树种的苗期生长均有明显的促生效果。接种270d后，3个树种接种处理苗期的苗高、地径、地下干质量和地上千质量等生长指标均显著优于未接种对照苗的各生长指标。方差分析结果表明，红锥接种苗的苗期各生长指标极显著优于未接种对照苗；黎蒴和木荷接种苗苗期的各生长指标与未接种对照苗有显著差异。菌根感染强度及依赖性分析结果表明，红锥的感染强度为4级，属强依赖性；黎蒴与木荷的感染强度为一般，属弱依赖性。红锥树种可与两个红菇菌形成较佳的共生体，其中以鳞盖红菇的接种效果为最佳。     

小叶锦鸡儿播种苗结瘤特性的研究

对小叶锦鸡儿播种苗结瘤特性定期观测结果表明,小叶锦鸡儿播种苗的根瘤形状各异,多为棒状,主要分布在侧根上。1年生播种苗根瘤多集中分布在地面以下10~20 cm的根系范围内,平均结瘤率为85%。随着生长时间的延长,主根上的根瘤数量逐渐减少,而侧根上的根瘤数量则不断增加,在8月中旬开始出现结瘤高峰。随着生长年限的增加,小叶锦鸡儿的结瘤能力不断提高,但由于栽植密度的增加,结瘤数量则不断减少。土壤养分和水分条件对小叶锦鸡儿1年生播种苗单株结瘤量有一定的影响。施用N、P、K复合肥后,1年生播种苗单株结瘤数量比对照减少了25%,控水处理与对照相比结瘤数量减少了45.6%。施用根瘤菌接种剂使小叶锦鸡儿1年生播种苗的单株结瘤数量比对照提高了39.2%,播种苗株高、地上部分干重和总干重分别比对照提高了12%、18.4%和13.1%。小叶锦鸡儿播种苗的结瘤量与多项生长指标呈显著的相关性。     

粗枝木麻黄接种弗兰克氏菌的研究

试验结果表明：粗枝木麻黄接种弗兰克氏菌对提高其生长量效果显著。接种不同弗兰克氏菌菌株的粗枝木麻黄苗木的生物量。根瘤重量和数量及叶片Ｎ含量均表现出明显的差异，其中以８８０５２、８７０６６和８７２８７菌株接种效果最好。在接菌与施肥的试验中，接菌不施肥处理的苗木叶片Ｎ含量最高。     

Effect of Frankia inoculation on the growth of Alnus sieboldiana on unsterilized soil

The effect of inoculation with Frankia, a N-fixing actinomycete, on the growth of Alnus sieboldiana seedlings was studied on unsterilized soil from a nursery and an alder stand (forest of Alnus firma). The seedlings of A. sieboldiana were inoculated with Frankia before or after a 2-month culture on sterilized vermiculite, during which they nodulated, and transplanted to unsterilized soil from the nursery and the alder stand. The control seedlings were also cultured on sterilized vermiculite for about 2 months and transplanted to unsterilized soil without Frankia inoculation. The seedling growth, nodulation and N-fixing activity were measured 3, 10 and 16 weeks after the transplantation. Growth and nodule biomass of the seedlings inoculated with Frankia and those grown on the alder soil were better than those without inoculation with Frankia and grown on the nursery soil, respectively. The seedlings inoculated before spontaneous nodulation grew better than those inoculated at the transplantation. Nitrogen-fixing activity measured by acetylene reduction assay at 16 weeks after the transplantation was higher in the seedlings grown on the soil from the nursery than on the soil from the alder stand.     

桉树接种固氮菌效果的研究

试验表明接种固氮菌能显著提高桉树苗木的生长及苗木各器官的生物量和Ｎ含量,接种苗木高生长比对照（不接种）增加２９．８１％、叶片数增加３７．３１％、叶片干重增加３５．０１％、茎干重增加５５．５９％、根干重增加７８．９３％、总生物量干重增加５５．８９％。不同菌株的增产效果存在差异,接种催娩克氏菌的效果较接种阴沟肠杆菌的好。接种＋施Ｎ８０ｍｇ／株处理的效果优于接种＋施Ｎ３０ｍｇ／株处理的效果。接种与施Ｎ肥的分析结果表明接种催娩克氏菌的效果等于施Ｎ８０ｍｇ／株的效果。     

Peat carrier increases inoculation success with Frankia on red alder (Alnus rubra Bong.) in fumigated nursery beds

Inoculation trials were set up in fumigated nursery beds for red alder (Alnus rubra Bong.) bare-root seedling production. Frankia inoculum was applied either as nodule homogenate or as pure culture (strain ArI5). The plots were laid out in 4 blocks of 8 treatments consisting of: control, nodule suspension, and three levels each of cell suspension and cells applied with a peat carrier. Numbers, height, and percentage nodulation on the seedlings were determined at mid-season. Numbers, size, dry weights, and degree of nodulation were determined at lifting. The peat inoculum treatment produced larger seedlings than the other treatments, both at mid-season and at lifting. The other treatments had little effect on growth. Size differences paralleled differences in degree of nodulation. Differences in percentage of seedlings nodulated were most pronounced at midseason, indicating that inoculation confered primarily an early-season advantage.     

木麻黄根瘤内生菌纯培养接种效果试验*

用Ｆｒａｎｋｉａ纯培养接种木麻黄苗木试验，获得了明显效果，苗高、地径、根瘤数、根瘤鲜重及生物量均比对照高。在４个供试菌株中，接种Ｂｒ的效果最好，苗高、地径分别为对照的３．５倍和２．７倍，其次是Ｐ１。接种效果随着苗龄增加越来越明显。苗木的高度、地径、生物量与根瘤数量、根瘤重呈极显著的直线正相关。     

几种豆科树种苗木结瘤及对其生长的影响研究

以实验室条件下分离培养得到的山毛豆、台湾相思、银合欢、圣诞树和刺槐根瘤菌分别接种山合欢、台湾相思、滇合欢、香须树等树种幼苗，结果表明，被接种苗木都形成了有效根瘤，其苗高、单株重量、生长状况明显高于对照，但同一树种对不同菌株的响应不同．     

西澳粘滑菇在尾叶桉上的菌根合成*

利用经特殊培养的西澳粘滑菇菌种，人工接种在尾叶桉幼苗根部，３５ｄ后有６０％的幼苗根部都长出１～２个子实体，经鉴定，该子实体为原接种菌。菌根感染率检查结果表明，有８０％幼苗形成菌根。生长８个月的幼苗其平均树高比未接种苗增加５２．２％，地径增加２９．４％，地上及地下部分生物量（干重）分别比对照增加２６８％及２１４．３％。西澳粘滑菇不仅在华南地区桉树上可以完成菌根合成，而且对幼树生长的增产效果十分明显，是华南地区桉树的优良菌根菌。     

杜鹃花属植物内生真菌对毛棉杜鹃幼苗生长的影响

对来自粤北3种野生杜鹃花属植物根样中的聚多曲霉（Aspergillus sydowii）（菌株号：YJ1,YS2）、杂色曲霉A. versicolor（菌株号：GD1）3个内生真菌菌株及其混合菌剂进行瓶内接种试验,比较其对毛棉杜鹃（Rhododendron moulmainense）幼苗的菌根侵染率、苗高和生物量的影响。结果表明,3个菌株及其混合菌剂均可与毛棉杜鹃幼苗根系形成菌根共生体,但不同菌剂在不同培养基上对毛棉杜鹃幼苗根系的侵染力、苗高增长和生物量增重效果存在显著差异（P<0.05）。多数菌剂（YS2、M3、M1、M2）在MMN培养基上对幼苗根系侵染力显著高于WPM培养基和混合基质培养基（P<0.05）,而多数接菌苗（YJ1、GD1、M2、M3）在WPM培养基上生长显著优于MMN培养基（P<0.05）,YS2、GD1和混合菌剂M2（YS2+GD1）对毛棉杜鹃幼苗的增高和增重具有明显的促生作用。     

Pt菌剂对高阿丁枫苗木生长的影响

进行高阿丁枫接种Pt菌剂的育苗效果试验。结果表明:Pt菌剂对高阿丁枫苗木的生长有明显的促进作用。经Pt菌剂处理后高阿丁枫苗木的苗高、地径、侧根数、主根长、地上部分鲜重、地下部分鲜重、全重及一级侧根长分别较不经Pt菌剂处理的对照提高了23.1%、8.7%、23.1%、21.0%、25%、33.3%、26.7%、49.2%。且苗木的径高比不失衡。     

Inoculation of Acacia holosericea with ectomycorrhizal fungi in a semiarid site in Senegal: growth response and influences on the mycorrhizal soil infectivity after 2 years plantation

The main objectives of this study were to determine the influence of several strains of ectomycorrhizal fungi on the growth of Acacia holosericea in a dry tropical environment and their short-term effect on indicators of ecosystem soil conditions such as biological soil properties (microbial biomass, mycorrhizal soil infectivity). Three fungal strains have been tested: Pisolithus albus IR100, P. albus COI024 and Scleroderma dictyosporum IR109. Ectomycorrhizal inoculation has significantly enhanced the growth (shoot and root biomass) of A. holosericea seedlings after 4 months culture in glasshouse conditions in a disinfected soil. In field conditions, the transplanting shock was less important for the ectomycorrhized trees. After 2 years, the inoculated trees had a better growth than that recorded in the control treatment. Ectomycorrhizal inoculation has significantly stimulated height, leave and wood biomass, root biomass of A. holosericea trees. Moreover, this fungal inoculation has significantly modified the leaf nutrient contents for P, N and phenols. Microbial biomass and mycorrhizal soil infectivity were also larger in the inoculated plots.Ectomycorrhizal inoculation could be of great relevance to improve the reafforestation process of degraded areas with legumes fast growing trees. This biological practice could also ameliorate soil characteristics such as microbial biomass or mycorrhizal soil infectivity.