冷库中低温细菌的分离及鉴定

从来自冷库中的样品中共筛选分离出5株低温细菌。对分离的低温细菌进行了分类鉴定,主要进行了生理、生化特性分析,对其中的2株细菌作了16S rDNA测序并通过BLAST比对进行了鉴定。生化鉴定结果表明：所有分离到的低温细菌均为革兰氏阴性菌。经16S rDNA鉴定的3株低温细菌全部为假单孢菌属的菌株。     

信息:植物保护与土壤肥料

广州市花卉病虫害发生特点及检疫措施；花卉三大害虫的防治；广州城区行道树受桑寄生侵害的调查研究；石斛兰的主要虫害及其防治；黄单胞菌引致红掌疫病的症状和病原菌鉴定；火鹤花衰退病诊断与检疫；热带植物花卉红掌叶腐病病原菌的分离与初步鉴定；金叶假连翘叶斑病发病规律及防治技术研究；棕榈科观赏植物真菌病害鉴定；近些年几种优秀杀菌剂农药推荐；园林植物害虫“无公害”防治技术；应用木霉制剂防治几种药用植物病害的研究；城市土壤对园林植物生长的影响及其改善措施；纳米肥料问世；有机营养肥料研究进展；     

浙江省南湖林场试验点油茶无性系鉴定试验总结

浙江省油茶优树鉴定协作组在多年油茶选优工作的基础上,自1979年起对决选的优良单株在龙游林场、常山油茶所、南湖林场等地进行当代鉴定试验。经几年的观察测定,于1983年对初选的优良无性系在丽水、龙游、常山、安吉等县进行无性系区域性试验.现将南湖林场试验点的油茶当代鉴定试验整理如下:     

“幼林抚育作业机”通过鉴定

浙江省林科所研制的“幼林抚育作业机”由省林业厅主持于1983年12月13日至16日在浙江省杭州市通过鉴定。“幼林抚育作业机是采用永康拖拉机厂生产的多用底盘,可配套五种作业机具,包括旋耕式中耕机、挂结式挖穴机、牵引式喷雾、喷灌两用森保车、电动割灌机和电动高杆整枝机。     

西双版纳红掌细菌性叶疫病病原菌初步研究

经鉴定,云南西双版纳的红掌细菌性叶疫病病原菌为Burkholderia caryophylli、Burkholderiasolanacearum、Pseudomonas sp.、Xanthomonas sp.等4个种。这4种菌的混合侵染力极强,目前在栽培管理上还缺乏有效的防治措施。     

园林花木盛会发展商机无限--2003首届中国浙江园林花木信息交流会在杭州举行

本刊讯(本刊记者缪宏特约记者万如伟)11月15日,由浙江省工商联园林花木商会、杭州市总商会园林花木商会主办,杭州蓝天园林集团承办的“2003首届中国浙江园林花木信息交流会”在杭州市武林广场隆重开幕。国家林业局经济发展研究中心致电祝贺;浙江省工商联党组副书记常务副会长汤为平、杭州市人大副主任安志云和国家林业局有关部门的负责人及120余家参展企业参加了开幕式。杭州市统战部副部长、市工商联党组书记赵天行、浙江省科技勘测设计处处长恽稚荣在开幕式上致辞。他们表示此次交流会对提高浙江花木的知名度和美誉度、创建浙江花木品牌起…     

硬叶兜兰菌根真菌的形态学特征与鉴定

将采集获得的野生硬叶兜兰植株,采用新单菌丝团分离法,分离纯化后,获得6株菌根真菌。对6株内生菌的菌落形态、产孢结构和分生孢子形状、大小作了测量与观察及一些生物学特性初步鉴定,结果为5株菌根真菌分别属于瘤菌根菌属(Epulorhiza)、胶膜菌属(Tulasnella)和镰刀菌属(Fusariu),未鉴定1种。     

年产20000t新型防水剂生产基地建成投产

由林产工业规划设计院临安化工制剂厂研制开发的新型人造板防水剂,先后被列入国家攻关(重点)计划、浙江省重点技术创新计划、杭州市重大科研计划,并获得了发明专利权.产品分别通过了科研项目结题鉴定和投产鉴定,专家的鉴定结论为:该产品生产工艺先进、产品性能独特,是对人造板防水材料和防水技术的一大突破,属国内首创,达到国际先进水平.     

开化林场杉木良种繁殖新技术

浙江省开化林场科技人员密切结合生产,在杉木选优的基础上,开展了杉木无性系快速繁殖试验,经过反复探索,终于研究成功快速繁殖新技术,获得了良好的经济效益与社会效益。已由浙江省科委和林业厅联合邀请省内外专家,通过了鉴定。该技术是利用杉木优株的萌蘖条进行扦插繁殖,一年四季均可进行。一株杉木优株四年内就可繁殖出壮苗二十万株,且无性繁     

主要林木种子贮藏及休眠研究通过鉴定

经浙江省林业厅批准,由浙江省林科所主持的“主要林木种子贮藏及休眠研究”,已于1991年12月13日通过专家鉴定。浙江省测试技术研究所、杭州市植物园,桐乡县林科站协作参加此项研究,研究的树种有杉木、池杉、马尾松、深山含笑、红豆杉、玉兰及浙江楠等主要造林树种和珍稀树种。该课题应用当代测试技术和植物生理理论,研究了种子休眠生理、劣变种子的细胞超微结构、     

西双版纳盆栽红掌温室大棚栽培技术

通过实践总结出西双版纳盆栽红掌大棚栽培管理技术,为红掌生产企业及花农提供技术支撑。     

红掌无土栽培基质的筛选

以红掌‘火焰’为试材,"花多多"有机肥代替营养液,进行国产泥炭、进口泥炭、中药渣、珍珠岩等栽培基质筛选试验。采用随机区组试验设计,以进口泥炭为对照,分析比较8种基质配方对红掌生长生理的影响。结果表明,试验范围内的红掌栽培基质筛选,以进口泥炭∶中药渣∶珍珠岩为2∶1∶1时最佳。     

浅谈红掌的生产栽培技术

从红掌栽培要求高温高湿、光照适中和精细水肥管理特点出发，结合广州地区的气候特点，总结自身的栽培实际经验，重点总结了温湿度、光照、水肥控制等方面的数据及各因子的相关性，根据自身的经验阐述了在广州地区进行红掌种植的生产栽培流程过程和生产管理技术。     

Mutualistic symbiosis between Bursaphelenchus xylophilus and bacteria of the genus Pseudomonas

Interactions between the pine wood nematode (PWN), Bursaphelenchus xylophilus, and bacteria of the genus Pseudomonas were examined by cultivating axenic PWN and bacterial strains using callus of Pinus thunbergii. Ten (Pseudomonas fluorescens, Pseudomonas putida, Pseudomonas cepacia and Pseudomonas spp.) of the 29 bacterial strains tested, significantly increased the reproduction of PWN. The rest of the bacteria (19 strains of 10 species) inhibited the reproduction of PWN completely. The growth of 18 of the 29 bacterial strains tested, including the 10 strains promoting PWN reproduction, was significantly increased by the presence of PWN. It indicated a mutualistic symbiotic relationship between PWN and the 10 bacterial strains in the genus Pseudomonas. The bacterial mutualistic symbionts are organisms, which may have co‐evolved with PWN rather than being accidentally associated. The finding provides further evidence for our hypothesis that pine wilt disease is complex, induced by both PWN and associated phytotoxin‐producing bacteria.     

景宁3个野生灵芝菌株的评比试验

对采自景宁畲族自治县的BL09、BL11、BL12三个野生灵芝菌株与当前景宁畲族自治县主栽品种韩芝进行子实体、生物学效率比较,结果表明,4个菌株在母种培养基上,BL09、BL11、BL12之间生长速度差异不显著,而韩芝生长较慢,并与其它菌株存在极显著的差异;BL09和BL11菌丝生长浓密洁白,气生菌丝量大,菌丝边缘整齐,BL12和韩芝则相对较差;4个菌株在原种培养基上的生长速度,BL09和BL11之间差异不显著,BL09、BL11与BL12、韩芝之间存在极显著差异,BL12和韩芝之间差异不显著;菌盖大小为BL09＞ BL11＞ BL 12＞韩芝,产孢量大小为BL09＞ BL11＞韩芝＞BL12; BL09和BL11产量较高,它们与韩芝和BL12之间存在显著差异,BL12产量最低,评比结果表明,BL09无论在菌丝生长方面还是在子实体生长方面,均优于其它菌株,都表现出良好的长式,是较适宜于景宁生产推广的优良菌株.     

PGPB对红树植物木榄幼苗的接种效应

通过盆栽试验研究了植物促生菌(PGPB)对红树植物木榄幼苗的接种效应,供试菌为6种解磷菌(即B.amy,Vib,B.atr,Xan,B.Lic,P.M.)和5种固氮菌(即Au4,Phy,24S,JA4,cd),结果表明:(1)分别接种6种解磷菌株6个月后木榄幼苗的生长均得到了不同程度的促进,与对照相比,木榄苗高增加21.57%～9.54%,生物量增加27.49%～20.32%,叶片的氮含量增加33.33%～1.35%,叶片的磷含量增加24.42%～2.04%;经差异显著性检验,B.amy和Vib 2个菌种对木榄幼苗的促生效应比其它解磷菌株更明显;(2)分别接种5种固氮菌株6个月后木榄幼苗的生长均得到了促进,与对照相比,木榄幼苗的均高增加24.07%～10.22%,生物量增加32.36%～19.71%,叶片的氮含量增加23.39%～4.05%,叶片的磷含量增加32.79%～4.99%;差异显著性检验表明:Au4和Phy 2个菌种对木榄幼苗的促生效果比其它固氮菌更显著.     

西南桦苗木猝倒病生防菌的筛选及鉴定研究

采用组织分离法和稀释平板法对西南桦猝倒病发生区健康苗木组织、根际土壤及咖啡壳堆肥化基质进行菌种分离和分子鉴定。将分离获得的17株菌株与西南桦苗木猝倒病病原菌尖孢镰刀菌和腐皮镰刀菌进行平板对峙实验,筛选病原拮抗菌。结果表明,筛选出的真菌5#、7#及细菌BJ、JCl对西南桦苗木猝倒病原菌尖孢镰刀菌和腐皮镰刀菌具有较强抑制效果。其中真菌5#和7#的抑菌率分别为91．30％和90．12％;细菌BJ和JCl抑菌带较宽,对峙4天分别达0．4cm和0．5cm,抑菌率分别为80．4％和78．6％,且BJ抑菌效果更好;真菌与细菌比较,拈抗真菌5#和7#比拮抗细菌BJ和JCl抑制热区西南桦苗木猝倒病菌效果更好。通过对峙实验筛选出的5#、7#、BJ和JCl拮抗菌,对热区防治苗木猝倒病及生防制剂的开发应用具有参考价值。     

杨梅弗兰克氏菌生物学特性研究

用根瘤切片法从浙江省多个产地的荸荠种、东魁种和深红种杨梅根瘤中分离得到5个杨梅弗兰克氏菌株,对其进行形态培养、生理类型和营养源利用等生物学特性的研究.结果表明,菌株在QMOD和BAP培养基中生长良好;生理类型试验参试的五株菌株中有2株菌株为生理B型,2株为生理A型,1株为生理AB型;菌株在营养源试验中,表现出以丙酮酸钠为唯一碳源或分别以酪蛋白、蛋白胨为唯一氮源的BAP培养基中生长良好.     

Evaluation of endophytic bacterial strains as antagonists of take-all in wheat caused by <Emphasis Type="Italic">Gaeumannomyces graminis</Emphasis> var. <Emphasis Type="Italic">tritici</Emphasis> in greenhouse and field

Six endophytic bacterial strains from wheat roots, selected from extensive screening tests, were studied in greenhouse pot experiments against take-all disease of wheat, caused by Gaeumannomyces graminis var. tritici (Ggt). For comparison, the fungicide triadimefon was included. All six bacterial strains and triadimefon significantly reduced take-all in wheat plants between 39.4 and 57.3% compared to the inoculated control. Treatment with the bacterial strains also significantly increased growth parameters of roots (e.g., average length, fresh, and dry weight) in comparison with the inoculated control. One of the endophytic bacterial strains, designated EDR4, which proved to be most consistent in further greenhouse tests, was also examined in field trials against take-all in wheat. In the field experiment 2006/2007, height of wheat plants in Ggt inoculated plots was significantly reduced compared to the non-inoculated treatments. Yield parameters such as kernels per head and thousand kernel weight (TKW) in the inoculated control were lower compared to other treatments. In the inoculated plots, individual treatments with the strain EDR4 increased yield compared to the inoculated control. In the field trial 2007/2008 treatments with strain EDR4 as well as the fungicide triadimefon reduced take-all by 49.6 and 61.9%, respectively, compared to the inoculated control. At harvest, in the inoculated control plant height, seeds per head and TKW were significantly lower compared to the other treatments. Treatments with EDR4 eliminated the detrimental effects of take-all on grain yield to a similar degree as triadimefon application. The strain EDR4 distinctly inhibited growth of Ggt in vitro and was identified as Bacillus subtilis on the basis of its morphology and 16S rRNA sequence analysis as well as physiological and biochemical characteristics. These results indicate that the strain EDR4 has potential in directly controlling take-all disease of wheat.